Click here to switch to the map view.

The map label for this gene is rluE [H]

Identifier: 124022563

GI number: 124022563

Start: 773374

End: 773985

Strand: Reverse

Name: rluE [H]

Synonym: P9303_08541

Alternate gene names: 124022563

Gene position: 773985-773374 (Counterclockwise)

Preceding gene: 124022565

Following gene: 124022557

Centisome position: 28.85

GC content: 55.72

Gene sequence:

>612_bases
TTGAACAAGCCCTACGGCGTCCTAAGCCAGTTCACCCCAGAACCAGGCAGCCAGTGGAGGTGCTTAGCAGACTTGATCCC
CATACCCAGGGTGTATGCGGCAGGCCGCCTAGATGCTGACAGTGAAGGCCTATTGGTTCTCACTGCCCATGGGCGCCTGC
AGCATCGACTCACCAATCCCCATTTGAGCCACTGGCGGGTTTATTGGGTGCAAGTAGAAGGTTCTCCTAGTGAAAGTCAA
CTACAAAAACTTGCCTCAGGAGTGATCATTCAGGGAAAGAGAACCCGTCCTGCCCTAGTGAGCTGGCTGAAGGGAAAAGC
TGCTCCGATCGTGAGAGAGCGTGACCCACCCATCCGTGTGCGGCAAAGCATCCCCACTAGCTGGCTTGAATTGAAGTTAA
AAGAAGGGAGAAACAGACAGGTTCGACGGATGACAGCAGCAGTGGGGCTGCCAACCCTTCGCTTGATCCGTCATCGCATC
GAGCTTATGGATGGTGAGCCACCCCTCAGCATTGAAGGGCTGGATCCTGGCCAATGGAGGCAGGTCTCATCAAGAGAGAC
CAACCTCCTGCTGTCCCTCCTCAATCGTCCGGCAGCTGACCCACAAGCGTGA

Upstream 100 bases:

>100_bases
AGCGCTGACGATCCCTACAACGATCTATCTATGCTGACACTGCTCGCGTCTACTGACTGATCAGCTGACCACCCAAGCAG
ACGACTTCACGACCCTTCTG

Downstream 100 bases:

>100_bases
CGGCGCGAAATTCAATGTCTTCAACCACCTGCCAGGGTTGATCAGAACGATCGGCATAGCTCACCTTCTCAAAGCCCAAA
GCACGAAAATCCTCTAGGAA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MNKPYGVLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLTAHGRLQHRLTNPHLSHWRVYWVQVEGSPSESQ
LQKLASGVIIQGKRTRPALVSWLKGKAAPIVRERDPPIRVRQSIPTSWLELKLKEGRNRQVRRMTAAVGLPTLRLIRHRI
ELMDGEPPLSIEGLDPGQWRQVSSRETNLLLSLLNRPAADPQA

Sequences:

>Translated_203_residues
MNKPYGVLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLTAHGRLQHRLTNPHLSHWRVYWVQVEGSPSESQ
LQKLASGVIIQGKRTRPALVSWLKGKAAPIVRERDPPIRVRQSIPTSWLELKLKEGRNRQVRRMTAAVGLPTLRLIRHRI
ELMDGEPPLSIEGLDPGQWRQVSSRETNLLLSLLNRPAADPQA
>Mature_203_residues
MNKPYGVLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLTAHGRLQHRLTNPHLSHWRVYWVQVEGSPSESQ
LQKLASGVIIQGKRTRPALVSWLKGKAAPIVRERDPPIRVRQSIPTSWLELKLKEGRNRQVRRMTAAVGLPTLRLIRHRI
ELMDGEPPLSIEGLDPGQWRQVSSRETNLLLSLLNRPAADPQA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=190, Percent_Identity=51.0526315789474, Blast_Score=166, Evalue=9e-43,
Organism=Escherichia coli, GI1790453, Length=200, Percent_Identity=29, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787524, Length=165, Percent_Identity=33.9393939393939, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788510, Length=190, Percent_Identity=31.0526315789474, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 22868; Mature: 22868

Theoretical pI: Translated: 11.23; Mature: 11.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPYGVLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLTAHGRLQHRLTNP
CCCCCCCHHHCCCCCCCCCCHHHHHCCCCCHHCCCCCCCCCCCEEEEEECCCEEHHCCCC
HLSHWRVYWVQVEGSPSESQLQKLASGVIIQGKRTRPALVSWLKGKAAPIVRERDPPIRV
CCCCEEEEEEEEECCCCHHHHHHHHCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCHH
RQSIPTSWLELKLKEGRNRQVRRMTAAVGLPTLRLIRHRIELMDGEPPLSIEGLDPGQWR
HHCCCCHHEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHH
QVSSRETNLLLSLLNRPAADPQA
CCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNKPYGVLSQFTPEPGSQWRCLADLIPIPRVYAAGRLDADSEGLLVLTAHGRLQHRLTNP
CCCCCCCHHHCCCCCCCCCCHHHHHCCCCCHHCCCCCCCCCCCEEEEEECCCEEHHCCCC
HLSHWRVYWVQVEGSPSESQLQKLASGVIIQGKRTRPALVSWLKGKAAPIVRERDPPIRV
CCCCEEEEEEEEECCCCHHHHHHHHCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCHH
RQSIPTSWLELKLKEGRNRQVRRMTAAVGLPTLRLIRHRIELMDGEPPLSIEGLDPGQWR
HHCCCCHHEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHH
QVSSRETNLLLSLLNRPAADPQA
CCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]