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The map label for this gene is cobQ

Identifier: 124022545

GI number: 124022545

Start: 759066

End: 760586

Strand: Reverse

Name: cobQ

Synonym: P9303_08361

Alternate gene names: 124022545

Gene position: 760586-759066 (Counterclockwise)

Preceding gene: 124022546

Following gene: 124022544

Centisome position: 28.35

GC content: 53.12

Gene sequence:

>1521_bases
ATGAGCACCGATGGGATGTCGACCCCCCTGATGGTGTTAGGCACATCCAGTGGAGCTGGTAAATCATTAATGTCGGCCGC
TCTCTGCAGGCTGCTGCTTCGGCGAGGCGAAACTCCCATACCCTTCAAAGGGCAAAACATGAGCAACAACGCCTGGGTTG
ATGAGAGCGGCGGAGAGATGGCCTATTCACAAGCCCTGCAAGCTTGGGCAGCAGGACTCAAGCCCCAATGCGCCATGAAC
CCTGTGCTGCTCAAACCCCAGGGAGACAGCACCAGTGAAGTCATCCATCTGGGTCAAAGCGTTGGCACGGCGCGTGCCGA
GACCTACTACGAGCAATGGTTCCGTCCTGGATGGAGCGTCATCCGTGAGGCGCTAAAAGACCTTCAGTGCTCTTACACCC
ATGGCCGGCTTGTCTTGGAAGGTGCAGGCAGCCCAGTGGAAGTCAACCTGCAACGGCGAGACCTCACCAACCTGCGATTG
GCTCAGTTCCTGCGTGCCCGCTGTTTGCTCGTTGCAGATATCGAACGCGGTGGTGTTTTTGCCCAGATCATTGGAACCCT
GTCATTACTGAAGCCGGTAGAAAAGCAATTGATACGCGGGCTACTCATCAATCGTTTCCGTGGCCGACGCGAACTTTTTG
ATGAAGGGCGCGATTGGCTAGAAGCCGAAACCGGTATTCCCGTCATCGGTGTAATGCCATGGCTGGAAGAGCTCTTCCCG
CCCGAAGACTCCCTTGATCTACTGGAGAGAAGAGGCAAAAAAAATGATGCCGAGATCGAAATCGCTGTCTTGAAATTGCC
ATCCCTAAGTAATTTTTCCGATCTCGATCCATTAGAAGCAGAACCCACAGTTCAGCTGCGATGGGTGGAACCCGGCACAT
TCCTCGGGACACCAGATGCTGTCATCATTCCCGGCAGCAAGCAGACACTGCGCGATCTAAATTCGCTAAACCGCAGTGGT
TTAGGGAGTCAGTTGCAAACCTACGCCCAAAGCGGTGGGCATGTGTTTGGCATCTGTGGCGGCATGCAAATGCTTGGGCG
AACTCTGGCAGATCCACTAGGTCTGGAAGGCGTCACAACAACAGACCCATCAAGCAGCATGGCAGGACTCAATCTGCTGC
CTCTAGATACCGTTTTCAAACGCAGTAAGGCTCTAAAACAATGCCAAAGGATTACCCAGTGGCCAGACAATGCCAAGGTG
AAGGGGTTTGAATTACACCATGGCAATAGCCAACTCATTCAAGGGAGCCATGAGTCTGTCCTACCTATGGCTGACGATCC
CTCACTGGGTTGGGTCAGTAAAAATGAGAGATGCGGCCAAGTCGCGGGAACTTACCTACACGGAATTTTCGAGAATGGTC
GCTGGCGAAGGCTGTGGCTCAATCTGATACGGAAGCAAAAGGGCTTAGCAGACTTGCCGACCGATATACCCCACCATGAG
CAACAAAGAGAACAACTACTCAATCGTCTCACCGATGTTTTCGAAGAACATGTCAACATCAACCCACTGCTGGGAACATG
A

Upstream 100 bases:

>100_bases
TGGGCCGCCAGCTACTTGAACAGTCCCCAGGGTTACCAACACAACGAGCTGGGATTTTCGAAAGACTGCAACACAATCTT
CGTCGACTTTGGCCCAGACA

Downstream 100 bases:

>100_bases
CGATCTCTAAAATTGAGATTCAATGGCCCAATGGCACAAGCTCCCTTACAGAACCAGGGCTTGATTGGCTCCAGGTTGCT
CGAGACGCAGGGATCATCAT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSTDGMSTPLMVLGTSSGAGKSLMSAALCRLLLRRGETPIPFKGQNMSNNAWVDESGGEMAYSQALQAWAAGLKPQCAMN
PVLLKPQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSVIREALKDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRL
AQFLRARCLLVADIERGGVFAQIIGTLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFP
PEDSLDLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGTPDAVIIPGSKQTLRDLNSLNRSG
LGSQLQTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTTTDPSSSMAGLNLLPLDTVFKRSKALKQCQRITQWPDNAKV
KGFELHHGNSQLIQGSHESVLPMADDPSLGWVSKNERCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDIPHHE
QQREQLLNRLTDVFEEHVNINPLLGT

Sequences:

>Translated_506_residues
MSTDGMSTPLMVLGTSSGAGKSLMSAALCRLLLRRGETPIPFKGQNMSNNAWVDESGGEMAYSQALQAWAAGLKPQCAMN
PVLLKPQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSVIREALKDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRL
AQFLRARCLLVADIERGGVFAQIIGTLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFP
PEDSLDLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGTPDAVIIPGSKQTLRDLNSLNRSG
LGSQLQTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTTTDPSSSMAGLNLLPLDTVFKRSKALKQCQRITQWPDNAKV
KGFELHHGNSQLIQGSHESVLPMADDPSLGWVSKNERCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDIPHHE
QQREQLLNRLTDVFEEHVNINPLLGT
>Mature_505_residues
STDGMSTPLMVLGTSSGAGKSLMSAALCRLLLRRGETPIPFKGQNMSNNAWVDESGGEMAYSQALQAWAAGLKPQCAMNP
VLLKPQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSVIREALKDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRLA
QFLRARCLLVADIERGGVFAQIIGTLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFPP
EDSLDLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGTPDAVIIPGSKQTLRDLNSLNRSGL
GSQLQTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTTTDPSSSMAGLNLLPLDTVFKRSKALKQCQRITQWPDNAKVK
GFELHHGNSQLIQGSHESVLPMADDPSLGWVSKNERCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDIPHHEQ
QREQLLNRLTDVFEEHVNINPLLGT

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_PROM3 (A2C7X7)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016852.1
- STRING:   A2C7X7
- GeneID:   4776611
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_08361
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 55921; Mature: 55790

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 339-339 ACT_SITE 447-447

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDGMSTPLMVLGTSSGAGKSLMSAALCRLLLRRGETPIPFKGQNMSNNAWVDESGGEM
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHH
AYSQALQAWAAGLKPQCAMNPVLLKPQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSV
HHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHH
IREALKDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRLAQFLRARCLLVADIERGGVF
HHHHHHHHCCCCCCCEEEEECCCCCEEEEEEHHCHHHHHHHHHHHHHHEEEEECCCCCHH
AQIIGTLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHCC
PEDSLDLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGTPDA
CCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCE
VIIPGSKQTLRDLNSLNRSGLGSQLQTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTT
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCCC
TDPSSSMAGLNLLPLDTVFKRSKALKQCQRITQWPDNAKVKGFELHHGNSQLIQGSHESV
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHCCCCCCCC
LPMADDPSLGWVSKNERCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDIPHHE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
QQREQLLNRLTDVFEEHVNINPLLGT
HHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STDGMSTPLMVLGTSSGAGKSLMSAALCRLLLRRGETPIPFKGQNMSNNAWVDESGGEM
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHH
AYSQALQAWAAGLKPQCAMNPVLLKPQGDSTSEVIHLGQSVGTARAETYYEQWFRPGWSV
HHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHHH
IREALKDLQCSYTHGRLVLEGAGSPVEVNLQRRDLTNLRLAQFLRARCLLVADIERGGVF
HHHHHHHHCCCCCCCEEEEECCCCCEEEEEEHHCHHHHHHHHHHHHHHEEEEECCCCCHH
AQIIGTLSLLKPVEKQLIRGLLINRFRGRRELFDEGRDWLEAETGIPVIGVMPWLEELFP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCCCEEEHHHHHHHHCC
PEDSLDLLERRGKKNDAEIEIAVLKLPSLSNFSDLDPLEAEPTVQLRWVEPGTFLGTPDA
CCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCE
VIIPGSKQTLRDLNSLNRSGLGSQLQTYAQSGGHVFGICGGMQMLGRTLADPLGLEGVTT
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCCC
TDPSSSMAGLNLLPLDTVFKRSKALKQCQRITQWPDNAKVKGFELHHGNSQLIQGSHESV
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHCCCCCCCC
LPMADDPSLGWVSKNERCGQVAGTYLHGIFENGRWRRLWLNLIRKQKGLADLPTDIPHHE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
QQREQLLNRLTDVFEEHVNINPLLGT
HHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA