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The map label for this gene is proS

Identifier: 124022453

GI number: 124022453

Start: 684878

End: 686677

Strand: Reverse

Name: proS

Synonym: P9303_07441

Alternate gene names: 124022453

Gene position: 686677-684878 (Counterclockwise)

Preceding gene: 124022455

Following gene: 124022452

Centisome position: 25.6

GC content: 54.94

Gene sequence:

>1800_bases
ATGCGCGTCTCCCGCCTGATGCTGGTGACGTTGCGCGACGTACCAGCAGACGCGGAAATCGCCTCCCATCAATTGCTGCT
CAGAGGGGGATACATCCGTCGCGTGACTTCAGGTATTTATGCCTACCTGCCCCTGATGTGGCGGGTCCTGCGCAAAATCA
CAGCGATTGTGCAGGAGGAAATGGATGCCACTGGTGCTCTGGAGACCTTGTTACCTCAATTGCAGCCTGCAGAGCTCTGG
CGGCGCAGTGGCCGATGGCAGGGGTACACGGCCGGCGAAGGCCTGATGTTCCACCTGGAAGACCGCCAAGGACGAGAACT
CGGCCTCGGCCCCACCCATGAAGAGGTGATCACAAGTCTTGCCGGAGACCTGCTGCGCTCATATCGACAGCTTCCTGTAA
CGCTCTATCAGATCCAAAGCAAATTCCGCGATGAGATCCGCCCACGTTTTGGCCTGATGCGCGGCCGGGAATTCATCATG
AAGGACGCCTATTCCTTCCATGCCTGCGAAGCAGACCTCGCCATGACCTACACAGCCATGGATGAGGCCTATCAACGAAT
CTTCTCCCGTTGCGGACTCGAAACCGTCGCTGTGGAAGCCGACAGCGGAGCCATCGGCGGAGCCTCGTCTCAAGAATTTA
TGGTCACAGCGGATGCTGGCGAAGACCTGATCCTGATCAGTGGTGATGGCCTCTACGCCGCCAACCAAGAAAAGGCGGTC
TCTCATCCACCAAAGGCCATCCCACTGCTTGCTTCAAAAGCTGCCTTGCTTGATACACCCGAACAGGGAACGATCGAGAC
ACTGTGTACCGCCCAGGGGCTTGTTCCGTCTCAAGTGGTGAAGGTGCTGGTCATGCTGGCCCGCATCGAAGATGGTGAAC
TTCAACCAGTCCTTGTAAGCATCCGAGGAGACCAGCAACTCAATGAGGTAAAGCTGATCAACGCCCTAAGCAGGGAACTA
AACAAGGGGGTGCTTGATGTGGCCCCCATCAGCGCCGATCAAATCAGTGCGCAAAAGCTTGAAGCCTGGCCCTTTGGGGC
GATGGGGCCTGACCTAGACGATGCCCTGTTAAGCGAAGCCACAAGCTGGACAAAACATTTCCTACGCCTAGCTGACCCGA
CAGCTACTGAGCTGAACTGTTTTGTCTGCGGTGCCAATCAAAACAATCAGCATCGAATTGGCATGACCTGGTCAAAGCTC
GGGGAAGTGCCCAAAAGCGTCGATCTACGCAAATCGCAGGCTGGCGATCGCTGTATCCATGACTCCAGCCAGATTCTGGA
GGAGAGAAGAGGCATTGAGGTAGGACACATTTTCCAATTAGGTCGCAAGTACTCAGAAGCACTAGAAGCCTGTTTCACCA
ATGACAAGGGCACTCAGGAACCTTTCTGGATGGGGTGTTACGGCATCGGGATTTCCAGACTTGCCCAGGCCGCTGTGGAA
CAACACCATGACGAAGCTGGCATGAATTGGCCTCTGGCTATAGCGCCATTTGAGGTCATCATCGTGGTAGCCAACATCCA
GGATGAGGTTCAGCAAAAGCTGGCCGAACAGCTTTACAGCGAACTACAAGCAGCTGATATCGAAGTGCTACTTGATGACA
GGAATGAACGCGCCGGCGTGAAATTCAAAGATGCTGACCTGATCGGGATCCCTTGGCGGATCGTGGTCGGCAGAGATGCC
GCCAAGGGGCAGGTGGAACTGATTCAAAGATCCAGCCGCAAGGTTGAAATACTTTCTGCAACACAGGCATTTACCGCACT
GGTTCAAGAGATTGCAGCGAACCAAAACACAAGGGTCTGA

Upstream 100 bases:

>100_bases
ACTCATCTCGCACAGGTCGATTCGGCGCCTTAAGACAGTTTCACGATCGGCTTTTTTGGACCGCGGATGGTAGTTTCCCC
CATCCACTGAGGTCCGATTC

Downstream 100 bases:

>100_bases
CCAGCCATTTAAAATTCCTGGAAATGGAATGAACCGGATGACTACTGCCTTGCATCACATGGCCAAGCAGTTGATGCGGG
CAGGATTGGCAATCTGCCTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MRVSRLMLVTLRDVPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITAIVQEEMDATGALETLLPQLQPAELW
RRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSLAGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIM
KDAYSFHACEADLAMTYTAMDEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGLYAANQEKAV
SHPPKAIPLLASKAALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARIEDGELQPVLVSIRGDQQLNEVKLINALSREL
NKGVLDVAPISADQISAQKLEAWPFGAMGPDLDDALLSEATSWTKHFLRLADPTATELNCFVCGANQNNQHRIGMTWSKL
GEVPKSVDLRKSQAGDRCIHDSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDKGTQEPFWMGCYGIGISRLAQAAVE
QHHDEAGMNWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEVLLDDRNERAGVKFKDADLIGIPWRIVVGRDA
AKGQVELIQRSSRKVEILSATQAFTALVQEIAANQNTRV

Sequences:

>Translated_599_residues
MRVSRLMLVTLRDVPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITAIVQEEMDATGALETLLPQLQPAELW
RRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSLAGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIM
KDAYSFHACEADLAMTYTAMDEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGLYAANQEKAV
SHPPKAIPLLASKAALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARIEDGELQPVLVSIRGDQQLNEVKLINALSREL
NKGVLDVAPISADQISAQKLEAWPFGAMGPDLDDALLSEATSWTKHFLRLADPTATELNCFVCGANQNNQHRIGMTWSKL
GEVPKSVDLRKSQAGDRCIHDSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDKGTQEPFWMGCYGIGISRLAQAAVE
QHHDEAGMNWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEVLLDDRNERAGVKFKDADLIGIPWRIVVGRDA
AKGQVELIQRSSRKVEILSATQAFTALVQEIAANQNTRV
>Mature_599_residues
MRVSRLMLVTLRDVPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITAIVQEEMDATGALETLLPQLQPAELW
RRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSLAGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIM
KDAYSFHACEADLAMTYTAMDEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGLYAANQEKAV
SHPPKAIPLLASKAALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARIEDGELQPVLVSIRGDQQLNEVKLINALSREL
NKGVLDVAPISADQISAQKLEAWPFGAMGPDLDDALLSEATSWTKHFLRLADPTATELNCFVCGANQNNQHRIGMTWSKL
GEVPKSVDLRKSQAGDRCIHDSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDKGTQEPFWMGCYGIGISRLAQAAVE
QHHDEAGMNWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEVLLDDRNERAGVKFKDADLIGIPWRIVVGRDA
AKGQVELIQRSSRKVEILSATQAFTALVQEIAANQNTRV

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=231, Percent_Identity=43.2900432900433, Blast_Score=189, Evalue=5e-48,
Organism=Escherichia coli, GI1786392, Length=594, Percent_Identity=44.2760942760943, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI115532348, Length=207, Percent_Identity=39.1304347826087, Blast_Score=148, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320931, Length=206, Percent_Identity=38.8349514563107, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656200, Length=228, Percent_Identity=38.1578947368421, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PROM3 (A2C7N5)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016760.1
- ProteinModelPortal:   A2C7N5
- SMR:   A2C7N5
- STRING:   A2C7N5
- GeneID:   4777558
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_07441
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 66278; Mature: 66278

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSRLMLVTLRDVPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITAIVQEE
CCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDATGALETLLPQLQPAELWRRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSL
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHHHHH
AGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIMKDAYSFHACEADLAMTYTAM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHH
DEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGLYAANQEKAV
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCEEECCCCCCC
SHPPKAIPLLASKAALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARIEDGELQPVLVS
CCCCCCCHHHHCCHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IRGDQQLNEVKLINALSRELNKGVLDVAPISADQISAQKLEAWPFGAMGPDLDDALLSEA
ECCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
TSWTKHFLRLADPTATELNCFVCGANQNNQHRIGMTWSKLGEVPKSVDLRKSQAGDRCIH
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCHHHHCCCCCCCCCHHHCCCCHHHC
DSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDKGTQEPFWMGCYGIGISRLAQAAVE
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHH
QHHDEAGMNWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEVLLDDRNERAGV
HHHHHCCCCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
KFKDADLIGIPWRIVVGRDAAKGQVELIQRSSRKVEILSATQAFTALVQEIAANQNTRV
EEECCCEECCCEEEEECCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRVSRLMLVTLRDVPADAEIASHQLLLRGGYIRRVTSGIYAYLPLMWRVLRKITAIVQEE
CCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDATGALETLLPQLQPAELWRRSGRWQGYTAGEGLMFHLEDRQGRELGLGPTHEEVITSL
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHHHHH
AGDLLRSYRQLPVTLYQIQSKFRDEIRPRFGLMRGREFIMKDAYSFHACEADLAMTYTAM
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHH
DEAYQRIFSRCGLETVAVEADSGAIGGASSQEFMVTADAGEDLILISGDGLYAANQEKAV
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCEEECCCCCCC
SHPPKAIPLLASKAALLDTPEQGTIETLCTAQGLVPSQVVKVLVMLARIEDGELQPVLVS
CCCCCCCHHHHCCHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IRGDQQLNEVKLINALSRELNKGVLDVAPISADQISAQKLEAWPFGAMGPDLDDALLSEA
ECCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
TSWTKHFLRLADPTATELNCFVCGANQNNQHRIGMTWSKLGEVPKSVDLRKSQAGDRCIH
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCHHHHCCCCCCCCCHHHCCCCHHHC
DSSQILEERRGIEVGHIFQLGRKYSEALEACFTNDKGTQEPFWMGCYGIGISRLAQAAVE
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHH
QHHDEAGMNWPLAIAPFEVIIVVANIQDEVQQKLAEQLYSELQAADIEVLLDDRNERAGV
HHHHHCCCCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
KFKDADLIGIPWRIVVGRDAAKGQVELIQRSSRKVEILSATQAFTALVQEIAANQNTRV
EEECCCEECCCEEEEECCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA