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The map label for this gene is purA

Identifier: 124022451

GI number: 124022451

Start: 683003

End: 684322

Strand: Reverse

Name: purA

Synonym: P9303_07421

Alternate gene names: 124022451

Gene position: 684322-683003 (Counterclockwise)

Preceding gene: 124022452

Following gene: 124022450

Centisome position: 25.51

GC content: 54.02

Gene sequence:

>1320_bases
GTGTCCTTGGCCAATGTTGTCGTCATCGGTGCGCAGTGGGGTGACGAAGGAAAAGGCAAGATCACCGACCTCCTTAGCCG
TTCAGCTGATGTTGTCGTCCGCTATCAAGGTGGCGTCAATGCCGGCCACACAATCGTTGTGGATGACAAGGTGCTCAAAC
TGCACCTCATTCCCTCAGGAATCCTTTACCCAGAAACAATCTGCCTAATCGGCTCTGGCACAGTCATCGATCCCAAAGTG
ATGCTCAAGGAGCTAGACATGCTGCTTGAGAACAGCATTGATATCTCAGGCCTTCAACTGGCGTCGACTGCCCACGTAAC
GATGCCCTATCACCGCCTCTTGGATGAGGCGATGGAGCAACAACGTGGCGATCAACGTATCGGCACTACTGGACGGGGCA
TCGGTCCCACCTATGCCGATAAATCCCAACGCAACGGCATTCGCGTACTTGATCTTTTAGATAGCCAACGACTACGAGAG
CGACTGCGTGGCCCTCTGCAAGAAAAGAATCGTCTACTCGAACAAATTTATGGTGTGGCACCACTTGACTCAGAGCAGGT
CATCGAGGAATACCTCGGCTATGGCCAAAGGCTTGCTCCACACGTTGTCGACTGTATTCAAACCATTCATCAAGCTGCCC
GCAGCCGCAAAAACATTCTTTTTGAAGGAGCTCAAGGGACCCTTTTAGACCTTGATCACGGCACCTATCCCTACGTCACC
TCCTCCAATCCAATCTCAGGCGGTGCATGCATCGGAGCTGGAGTCGGACCCACGCTGATCGATCGAGTGATCGGTGTTGC
CAAGGCTTACACCACCCGTGTAGGGGAAGGTCCTTTCCCAACAGAACTGGAAGGAAGCATCAATGAACAGCTCTGTGACC
GGGGAGGTGAATTCGGCACCACCACAGGCCGACGCAGACGCTGCGGCTGGTTCGATGGGGTCATTGGTCGTTACGCAGTA
GCAGTGAATGGCCTGGATTGCCTGGCTATTACCAAACTCGATGTGCTTGATGAGCTCGACGAAATCCGAGTGTGCGTCGC
CTACGAACTCAATGGTGAACGAATTGAACACTTCCCTAGCAGCGCAGAAGACTTTGCCCGCTGTCATCCAATCTTCGAGA
CCCTGCCAGGCTGGCAGTGCACCACTGCAGACTGCCGCCGTCTTGAAGACCTACCAACCACAGCGATGGACTATCTGCGC
TTCCTCGCCGACCTCATGGAAGTGCCGATTGCCATCGTCTCCCTAGGTGCCAACCGTGATCAAACAATCGTTGTAGAAGA
TCCGATCCACGGTCCCAAAAGGGCTCTCCTAAGCGCCTAA

Upstream 100 bases:

>100_bases
TAAGAGGCAGCTAAATCAACTGCTAGGCAATCGCTGTTTGACTTAAGGCAGGGATATCTGGGAAGATCCCCGTTCTGAAT
AATGGCGGCTTCGGGCCGCA

Downstream 100 bases:

>100_bases
GCCCTTTCTCAAAACAGATGGTGGACGGGGATCGATACTTCTGATGTTTCCCATGCATCCCTCCATGGCTATCCCCAGGT
TCAGTGATCGTCCTCTCGAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSLANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYPETICLIGSGTVIDPKV
MLKELDMLLENSIDISGLQLASTAHVTMPYHRLLDEAMEQQRGDQRIGTTGRGIGPTYADKSQRNGIRVLDLLDSQRLRE
RLRGPLQEKNRLLEQIYGVAPLDSEQVIEEYLGYGQRLAPHVVDCIQTIHQAARSRKNILFEGAQGTLLDLDHGTYPYVT
SSNPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSINEQLCDRGGEFGTTTGRRRRCGWFDGVIGRYAV
AVNGLDCLAITKLDVLDELDEIRVCVAYELNGERIEHFPSSAEDFARCHPIFETLPGWQCTTADCRRLEDLPTTAMDYLR
FLADLMEVPIAIVSLGANRDQTIVVEDPIHGPKRALLSA

Sequences:

>Translated_439_residues
MSLANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYPETICLIGSGTVIDPKV
MLKELDMLLENSIDISGLQLASTAHVTMPYHRLLDEAMEQQRGDQRIGTTGRGIGPTYADKSQRNGIRVLDLLDSQRLRE
RLRGPLQEKNRLLEQIYGVAPLDSEQVIEEYLGYGQRLAPHVVDCIQTIHQAARSRKNILFEGAQGTLLDLDHGTYPYVT
SSNPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSINEQLCDRGGEFGTTTGRRRRCGWFDGVIGRYAV
AVNGLDCLAITKLDVLDELDEIRVCVAYELNGERIEHFPSSAEDFARCHPIFETLPGWQCTTADCRRLEDLPTTAMDYLR
FLADLMEVPIAIVSLGANRDQTIVVEDPIHGPKRALLSA
>Mature_438_residues
SLANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYPETICLIGSGTVIDPKVM
LKELDMLLENSIDISGLQLASTAHVTMPYHRLLDEAMEQQRGDQRIGTTGRGIGPTYADKSQRNGIRVLDLLDSQRLRER
LRGPLQEKNRLLEQIYGVAPLDSEQVIEEYLGYGQRLAPHVVDCIQTIHQAARSRKNILFEGAQGTLLDLDHGTYPYVTS
SNPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSINEQLCDRGGEFGTTTGRRRRCGWFDGVIGRYAVA
VNGLDCLAITKLDVLDELDEIRVCVAYELNGERIEHFPSSAEDFARCHPIFETLPGWQCTTADCRRLEDLPTTAMDYLRF
LADLMEVPIAIVSLGANRDQTIVVEDPIHGPKRALLSA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=42.9577464788732, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI22748717, Length=424, Percent_Identity=44.1037735849057, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI40316944, Length=395, Percent_Identity=42.2784810126582, Blast_Score=303, Evalue=2e-82,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=47.0725995316159, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=43.1235431235431, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=43.1235431235431, Blast_Score=330, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=43.1870669745958, Blast_Score=340, Evalue=2e-94,
Organism=Drosophila melanogaster, GI21356033, Length=429, Percent_Identity=43.1235431235431, Blast_Score=348, Evalue=3e-96,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_PROM3 (A2C7N3)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016758.1
- ProteinModelPortal:   A2C7N3
- SMR:   A2C7N3
- STRING:   A2C7N3
- GeneID:   4776446
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_07421
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48029; Mature: 47897

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 15-15 ACT_SITE 43-43 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSG
CCCCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCC
ILYPETICLIGSGTVIDPKVMLKELDMLLENSIDISGLQLASTAHVTMPYHRLLDEAMEQ
CCCCCEEEEECCCCEECHHHHHHHHHHHHHCCCCCCCEEEECEEEEECHHHHHHHHHHHH
QRGDQRIGTTGRGIGPTYADKSQRNGIRVLDLLDSQRLRERLRGPLQEKNRLLEQIYGVA
HCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
PLDSEQVIEEYLGYGQRLAPHVVDCIQTIHQAARSRKNILFEGAQGTLLDLDHGTYPYVT
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCEEE
SSNPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSINEQLCDRGGEFGT
CCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCC
TTGRRRRCGWFDGVIGRYAVAVNGLDCLAITKLDVLDELDEIRVCVAYELNGERIEHFPS
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCEEEEEEECCCHHHHHCCC
SAEDFARCHPIFETLPGWQCTTADCRRLEDLPTTAMDYLRFLADLMEVPIAIVSLGANRD
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
QTIVVEDPIHGPKRALLSA
CEEEEECCCCCHHHHHCCC
>Mature Secondary Structure 
SLANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSG
CCCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCC
ILYPETICLIGSGTVIDPKVMLKELDMLLENSIDISGLQLASTAHVTMPYHRLLDEAMEQ
CCCCCEEEEECCCCEECHHHHHHHHHHHHHCCCCCCCEEEECEEEEECHHHHHHHHHHHH
QRGDQRIGTTGRGIGPTYADKSQRNGIRVLDLLDSQRLRERLRGPLQEKNRLLEQIYGVA
HCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
PLDSEQVIEEYLGYGQRLAPHVVDCIQTIHQAARSRKNILFEGAQGTLLDLDHGTYPYVT
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCEEE
SSNPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELEGSINEQLCDRGGEFGT
CCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCC
TTGRRRRCGWFDGVIGRYAVAVNGLDCLAITKLDVLDELDEIRVCVAYELNGERIEHFPS
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCEEEEEEECCCHHHHHCCC
SAEDFARCHPIFETLPGWQCTTADCRRLEDLPTTAMDYLRFLADLMEVPIAIVSLGANRD
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
QTIVVEDPIHGPKRALLSA
CEEEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA