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The map label for this gene is hemC

Identifier: 124022436

GI number: 124022436

Start: 672155

End: 673108

Strand: Reverse

Name: hemC

Synonym: P9303_07271

Alternate gene names: 124022436

Gene position: 673108-672155 (Counterclockwise)

Preceding gene: 124022438

Following gene: 124022430

Centisome position: 25.09

GC content: 56.5

Gene sequence:

>954_bases
ATGGTCCTCACCCAACTGCGCATCGCCTCACGCCGAAGCCAGCTGGCCATGGTGCAAACCAATTGGGTGCAAGCGGAACT
GGAGCAAGCACACCCTGGCCTTTCCATCTCGGTAGAAGCGATGGCAACCCAGGGAGACAAAATCCTCGATGTCGCCCTTG
CCAAGATCGGCGACAAAGGCCTGTTCACGAAAGAGCTTGAAGCTCAGATGCTGATCGGCCGTGCTGATATCGCTGTGCAT
TCCCTCAAAGATCTACCCACCAATCTTCCCGAAGGGCTCATGCTTGGATGCGTGACTGAGCGAGAAGACCCAGCCGACGC
TCTGGTGGTGAATCAAAAGAATGCCGAACATCAACTCGACACCCTGCCTGAAGGGGCAATCGTTGGCACAAGCTCGCTAC
GGCGCCTCGCCCAATTGCGGCATCACTACCCCCATCTGGTGTTTAAGGATGTGCGCGGCAATGTGATCACCCGCCTTGAA
AAACTGGATGCCGGCAACTATGACTGCCTGATCCTTGCAGCAGCAGGTCTGACAAGGCTTGGCTTTGGAGATCGCATTCA
TCAGTTGATTCCCAGCGAGATCTCCCTTCATGCCGTTGGCCAGGGAGCCCTAGGCATTGAATGCGTGGAGGGTCACCCCG
AAGTCCTCGAGGTCATCAAGGCACTGGAGCACAAACCAACAGCGCAGCGCTGCCTTGCTGAACGGGCCTTACTGAGAGAA
CTAGAAGGGGGCTGTCAGGTCCCCATCGGTGTCAATAGCCGCATCGAAGCCAATGAATTACTGCTCACGGGCATGGTTGC
CAGCTTGGATGGCAAACGCTTGATACGTGATCAGCAGCGCGGCCCAATTGATCGCTGCGAAGCCATCGGCAAGGAACTCG
CTGAAACCCTGAAATCTCAAGGCGCCGGAGAGATCCTTGCGGAAATCTTCGCTGCTGTCAGGCCAGAAGCCTGA

Upstream 100 bases:

>100_bases
CCGAAAAACCTCCCTTCCCTCAGAGCATTAACGGATTATCAGCCCATCGCGAAGATCGTGGGGTCGACTGCTTAATCTGG
ACTGCTGGAGTTCTGAACAC

Downstream 100 bases:

>100_bases
CTCCTCTCAATCTCTATTGAGCTAGCTAGCTAGATCAATCAAGCACTCGTCAGTCTGTTGGCTGCGCTGACAAGGATCAG
CGCAGGATTTCCACAGTTGT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MVLTQLRIASRRSQLAMVQTNWVQAELEQAHPGLSISVEAMATQGDKILDVALAKIGDKGLFTKELEAQMLIGRADIAVH
SLKDLPTNLPEGLMLGCVTEREDPADALVVNQKNAEHQLDTLPEGAIVGTSSLRRLAQLRHHYPHLVFKDVRGNVITRLE
KLDAGNYDCLILAAAGLTRLGFGDRIHQLIPSEISLHAVGQGALGIECVEGHPEVLEVIKALEHKPTAQRCLAERALLRE
LEGGCQVPIGVNSRIEANELLLTGMVASLDGKRLIRDQQRGPIDRCEAIGKELAETLKSQGAGEILAEIFAAVRPEA

Sequences:

>Translated_317_residues
MVLTQLRIASRRSQLAMVQTNWVQAELEQAHPGLSISVEAMATQGDKILDVALAKIGDKGLFTKELEAQMLIGRADIAVH
SLKDLPTNLPEGLMLGCVTEREDPADALVVNQKNAEHQLDTLPEGAIVGTSSLRRLAQLRHHYPHLVFKDVRGNVITRLE
KLDAGNYDCLILAAAGLTRLGFGDRIHQLIPSEISLHAVGQGALGIECVEGHPEVLEVIKALEHKPTAQRCLAERALLRE
LEGGCQVPIGVNSRIEANELLLTGMVASLDGKRLIRDQQRGPIDRCEAIGKELAETLKSQGAGEILAEIFAAVRPEA
>Mature_317_residues
MVLTQLRIASRRSQLAMVQTNWVQAELEQAHPGLSISVEAMATQGDKILDVALAKIGDKGLFTKELEAQMLIGRADIAVH
SLKDLPTNLPEGLMLGCVTEREDPADALVVNQKNAEHQLDTLPEGAIVGTSSLRRLAQLRHHYPHLVFKDVRGNVITRLE
KLDAGNYDCLILAAAGLTRLGFGDRIHQLIPSEISLHAVGQGALGIECVEGHPEVLEVIKALEHKPTAQRCLAERALLRE
LEGGCQVPIGVNSRIEANELLLTGMVASLDGKRLIRDQQRGPIDRCEAIGKELAETLKSQGAGEILAEIFAAVRPEA

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=331, Percent_Identity=44.7129909365559, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI66933009, Length=331, Percent_Identity=44.7129909365559, Blast_Score=268, Evalue=5e-72,
Organism=Escherichia coli, GI48994974, Length=315, Percent_Identity=51.1111111111111, Blast_Score=293, Evalue=8e-81,
Organism=Saccharomyces cerevisiae, GI6319996, Length=316, Percent_Identity=45.253164556962, Blast_Score=248, Evalue=7e-67,
Organism=Drosophila melanogaster, GI20130425, Length=284, Percent_Identity=45.4225352112676, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_PROM3 (A2C7L8)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016743.1
- ProteinModelPortal:   A2C7L8
- SMR:   A2C7L8
- STRING:   A2C7L8
- GeneID:   4777151
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_07271
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34379; Mature: 34379

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLTQLRIASRRSQLAMVQTNWVQAELEQAHPGLSISVEAMATQGDKILDVALAKIGDKG
CCEEHHHHHHHHHHEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCC
LFTKELEAQMLIGRADIAVHSLKDLPTNLPEGLMLGCVTEREDPADALVVNQKNAEHQLD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHH
TLPEGAIVGTSSLRRLAQLRHHYPHLVFKDVRGNVITRLEKLDAGNYDCLILAAAGLTRL
CCCCCCEECHHHHHHHHHHHHHCCCCEEHHHCCHHHHHHHHHCCCCCCEEEEEECCHHHC
GFGDRIHQLIPSEISLHAVGQGALGIECVEGHPEVLEVIKALEHKPTAQRCLAERALLRE
CCHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEGGCQVPIGVNSRIEANELLLTGMVASLDGKRLIRDQQRGPIDRCEAIGKELAETLKSQ
HCCCCEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
GAGEILAEIFAAVRPEA
CHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVLTQLRIASRRSQLAMVQTNWVQAELEQAHPGLSISVEAMATQGDKILDVALAKIGDKG
CCEEHHHHHHHHHHEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCC
LFTKELEAQMLIGRADIAVHSLKDLPTNLPEGLMLGCVTEREDPADALVVNQKNAEHQLD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHH
TLPEGAIVGTSSLRRLAQLRHHYPHLVFKDVRGNVITRLEKLDAGNYDCLILAAAGLTRL
CCCCCCEECHHHHHHHHHHHHHCCCCEEHHHCCHHHHHHHHHCCCCCCEEEEEECCHHHC
GFGDRIHQLIPSEISLHAVGQGALGIECVEGHPEVLEVIKALEHKPTAQRCLAERALLRE
CCHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEGGCQVPIGVNSRIEANELLLTGMVASLDGKRLIRDQQRGPIDRCEAIGKELAETLKSQ
HCCCCEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
GAGEILAEIFAAVRPEA
CHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA