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The map label for this gene is xthA [H]

Identifier: 124022407

GI number: 124022407

Start: 651527

End: 652366

Strand: Reverse

Name: xthA [H]

Synonym: P9303_06981

Alternate gene names: 124022407

Gene position: 652366-651527 (Counterclockwise)

Preceding gene: 124022409

Following gene: 124022405

Centisome position: 24.32

GC content: 52.5

Gene sequence:

>840_bases
GTGCAGATCGCTACCTGGAACGTCAACTCAATTCGCACCCGCCTCGATCAGGTGCAAGCTTGGCTACAGGATGCCCAACC
CGATCTGCTTTGCCTCCAGGAGACCAAAGTGGATGATCCGCTGTTCCCTCACGAGGTCTTTGAAGCTCAGGGCTATCAAG
TGCACTTTCACGGACAGAAGGCCTATAACGGCGTTGCAATCGTTAGCCGTCAACCTCTCGAAGATGTGCGCCGGGGCTTC
ACTGGCGAGCTCCCAGAGGATGCCGAAGCCTTGCAACTGGGAGAGCAAAAGCGCGTCATCAGCGCGCTCGTTAACAACAT
TCGTATCGTCAATGTCTACGTTCCCAACGGTTCCGCTCTGAAATCAGAGAAATATCCCTACAAACTCGAATGGCTGAGCT
GCCTAAACCGCTATTTGAGTGCACAAGCGAAACGGGATGAACCCCTCTGCCTCGTTGGGGATTTCAATATCGCCTTGGAA
GCAAGAGACATTCATCATCCCGAACGACTAACGGGAGGGATTATGGCTAGCGAGCTTGAAAGAGAGGCTCTTCTAAAGGT
TTTAGGAGACCGACTCCACGACGTGTTCCGCGTGTTCGAACCAGATGCCAACCATTGGAGCTGGTGGGACTATCGAAGTG
GTGCCTGGGACCGTGATCAGGGTTGGCGTATTGATCACATCTACCTATGCGATGAGCTTCTCAGTCAGGCCAGAAGCTGT
GTGATTCACAAACATCTAAGGGGCCATGAAAAGCCTAGTGATCATGCCCCGGTAACCGTAGATCTCAACTGGCCTCCAAT
TGATGACGATGAGGAGATGTCGGAACTGTTTGGCAATTGA

Upstream 100 bases:

>100_bases
GCAAAAGGGAATAAGACCTAAGGGGGCTCGGTTGATCAATGACGCATCCCCAAGTTCAGTAAGGCTAATGCTAATTGTCA
TGGTCAGATTTCCATGACCT

Downstream 100 bases:

>100_bases
AGGACATGGCTATTTAAGGCAATAGTCGCAGTCAAGGATTCACCTCCGGAGCAGTCAGAATGCCTTGGCTTCTGAAGGCA
TCTCAAGACCTTCTGAAGTC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MQIATWNVNSIRTRLDQVQAWLQDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQKAYNGVAIVSRQPLEDVRRGF
TGELPEDAEALQLGEQKRVISALVNNIRIVNVYVPNGSALKSEKYPYKLEWLSCLNRYLSAQAKRDEPLCLVGDFNIALE
ARDIHHPERLTGGIMASELEREALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
VIHKHLRGHEKPSDHAPVTVDLNWPPIDDDEEMSELFGN

Sequences:

>Translated_279_residues
MQIATWNVNSIRTRLDQVQAWLQDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQKAYNGVAIVSRQPLEDVRRGF
TGELPEDAEALQLGEQKRVISALVNNIRIVNVYVPNGSALKSEKYPYKLEWLSCLNRYLSAQAKRDEPLCLVGDFNIALE
ARDIHHPERLTGGIMASELEREALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
VIHKHLRGHEKPSDHAPVTVDLNWPPIDDDEEMSELFGN
>Mature_279_residues
MQIATWNVNSIRTRLDQVQAWLQDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQKAYNGVAIVSRQPLEDVRRGF
TGELPEDAEALQLGEQKRVISALVNNIRIVNVYVPNGSALKSEKYPYKLEWLSCLNRYLSAQAKRDEPLCLVGDFNIALE
ARDIHHPERLTGGIMASELEREALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
VIHKHLRGHEKPSDHAPVTVDLNWPPIDDDEEMSELFGN

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=29.2592592592593, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=29.2592592592593, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=29.2592592592593, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=37.037037037037, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71989536, Length=270, Percent_Identity=25.5555555555556, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI221330655, Length=268, Percent_Identity=28.7313432835821, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17136678, Length=268, Percent_Identity=28.7313432835821, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32184; Mature: 32184

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIATWNVNSIRTRLDQVQAWLQDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEECCCC
AYNGVAIVSRQPLEDVRRGFTGELPEDAEALQLGEQKRVISALVNNIRIVNVYVPNGSAL
CCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHCCEEEEEEEECCCCCC
KSEKYPYKLEWLSCLNRYLSAQAKRDEPLCLVGDFNIALEARDIHHPERLTGGIMASELE
CCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHH
REALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
VIHKHLRGHEKPSDHAPVTVDLNWPPIDDDEEMSELFGN
HHHHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQIATWNVNSIRTRLDQVQAWLQDAQPDLLCLQETKVDDPLFPHEVFEAQGYQVHFHGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEECCCC
AYNGVAIVSRQPLEDVRRGFTGELPEDAEALQLGEQKRVISALVNNIRIVNVYVPNGSAL
CCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHHHCCEEEEEEEECCCCCC
KSEKYPYKLEWLSCLNRYLSAQAKRDEPLCLVGDFNIALEARDIHHPERLTGGIMASELE
CCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHH
REALLKVLGDRLHDVFRVFEPDANHWSWWDYRSGAWDRDQGWRIDHIYLCDELLSQARSC
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
VIHKHLRGHEKPSDHAPVTVDLNWPPIDDDEEMSELFGN
HHHHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]