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The map label for this gene is hemL

Identifier: 124022405

GI number: 124022405

Start: 648562

End: 649848

Strand: Reverse

Name: hemL

Synonym: P9303_06961

Alternate gene names: 124022405

Gene position: 649848-648562 (Counterclockwise)

Preceding gene: 124022407

Following gene: 124022400

Centisome position: 24.22

GC content: 57.81

Gene sequence:

>1287_bases
TTGAACACCAGCCGCTCCCAGGCGATTTTCAGCGCCGCCCAGCGCTTAATGCCTGGTGGCGTTAGTTCTCCAGTGCGAGC
ATTCCGCTCCGTAGGCGGTCAACCGATCGTGTTTGACCGCGTAAAGGGTGCCTACGCCTGGGACGTCGATGGCAATCGCT
TCATCGACTACATCGGCAGCTGGGGGCCCGCAATCTGCGGCCATGCCCACCCAGAAGTCATCGCTGCTCTACAAGATGCG
CTTGAAAAGGGCACAAGCTTTGGTGCCCCATGTGAGCTAGAGAATCAACTGGCTGAAATGGTGATCGACGCCGTGCCCAG
CGTCGAGATGGTCCGTTTTGTGAATAGCGGCACTGAGGCCTGCATGTCTGTACTGCGCCTCATGCGCGCATTCACTGGCC
GCGACAAGCTCATCAAATTCGAAGGCTGCTATCACGGCCACGCCGACATGTTTTTGGTGAAAGCTGGATCCGGGGTGGCA
ACTCTCGGTCTACCCGACTCACCAGGGGTGCCCCGCAGCACCACCAGTAACACCCTCACTGCGCCCTACAACGATCTCGA
GGCAGTCAAAGAGCTGTTTGCCGAGAATCCTGACGCAATCAGCGGGGTGATCCTTGAGCCGGTCGTAGGAAATGCCGGAT
TCATTACTCCAGAGCCTGGTTTCCTGGAAGGTTTACGAGAACTCACCCGTGAACACGGAGCCTTGCTTGTGTTTGATGAA
GTCATGAGCGGGTTCCGCATCAGCTACGGAGGTGCTCAGGCCCGCTTTGGCGTCACGCCTGATCTAACCACCATGGGGAA
AGTGATCGGTGGTGGGCTCCCCGTTGGTGCTTATGGCGGCAGAGCCGAGATCATGGAGATGGTGGCCCCCGCAGGCCCCA
TGTACCAAGCCGGCACGCTGAGTGGCAACCCGCTGGCCATGACTGCTGGCATCAAGACCCTTGAGCTACTCAAGCAAGAA
GGCACCTATGAGCGCCTAGAAAGCACAACGGAGCGCTTAATTAACGGCATTCTCGAAGCAGCCAAAGCAGCAGAGGTTCC
CATTACGGGCAACAGCATCGGTGCGATGTTCGGCTTCTTCCTTTGCGAAGGCCCAGTACGGAATTTCGAAGATGCCAAAG
CAACCGATGCCGAACGCTTCGGCAAGCTGCATCGGGCCATGCTCGAAAGGGGCATCTACCTAGCCCCAAGTGCCTTCGAA
GCAGGCTTCACGTCCCTTGCCCATTCCGAAGCAGACATCGAAACCACCCTGAAGGCATTCCGCGAGAGTTTTGCAGCAGT
GGCGTAA

Upstream 100 bases:

>100_bases
GATGGCCTGACACAGTTCATCCGCTTCTGACCGTTTTGGGTCAAGATGGCGAACGGTTCATATCAGCCACTTGGCTTCGG
TCCCTGTGACAGCTTCAGCC

Downstream 100 bases:

>100_bases
ACAGACAACGAGAATAGAGAGCTGTTCTTACAGCTCTCCTGCGCAAATACAAACGAGCCGAAGCCAAGGTGCTACATCTA
GAACTAAAAGTTGAGAAGCG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTSRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGSWGPAICGHAHPEVIAALQDA
LEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEACMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTSNTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYERLESTTERLINGILEAAKAAEVPITGNSIGAMFGFFLCEGPVRNFEDAKATDAERFGKLHRAMLERGIYLAPSAFE
AGFTSLAHSEADIETTLKAFRESFAAVA

Sequences:

>Translated_428_residues
MNTSRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGSWGPAICGHAHPEVIAALQDA
LEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEACMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTSNTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYERLESTTERLINGILEAAKAAEVPITGNSIGAMFGFFLCEGPVRNFEDAKATDAERFGKLHRAMLERGIYLAPSAFE
AGFTSLAHSEADIETTLKAFRESFAAVA
>Mature_428_residues
MNTSRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGSWGPAICGHAHPEVIAALQDA
LEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEACMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVA
TLGLPDSPGVPRSTTSNTLTAPYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTLSGNPLAMTAGIKTLELLKQE
GTYERLESTTERLINGILEAAKAAEVPITGNSIGAMFGFFLCEGPVRNFEDAKATDAERFGKLHRAMLERGIYLAPSAFE
AGFTSLAHSEADIETTLKAFRESFAAVA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=315, Percent_Identity=29.8412698412698, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI13994255, Length=431, Percent_Identity=23.4338747099768, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI37574042, Length=335, Percent_Identity=26.865671641791, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI24119277, Length=370, Percent_Identity=27.2972972972973, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI226442705, Length=334, Percent_Identity=26.0479041916168, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI284507298, Length=160, Percent_Identity=33.75, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI226442709, Length=303, Percent_Identity=26.7326732673267, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=55.0588235294118, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1789016, Length=362, Percent_Identity=34.5303867403315, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788044, Length=312, Percent_Identity=34.2948717948718, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789759, Length=328, Percent_Identity=30.1829268292683, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787560, Length=323, Percent_Identity=30.9597523219814, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI145693181, Length=320, Percent_Identity=29.6875, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786991, Length=369, Percent_Identity=24.6612466124661, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=301, Percent_Identity=28.2392026578073, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=374, Percent_Identity=26.7379679144385, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI25144274, Length=148, Percent_Identity=33.7837837837838, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323470, Length=310, Percent_Identity=28.0645161290323, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324432, Length=363, Percent_Identity=23.9669421487603, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21357415, Length=300, Percent_Identity=26.3333333333333, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_PROM3 (A2C7I7)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016712.1
- ProteinModelPortal:   A2C7I7
- SMR:   A2C7I7
- STRING:   A2C7I7
- GeneID:   4776362
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_06961
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45474; Mature: 45474

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHCC
WGPAICGHAHPEVIAALQDALEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEA
CCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
CMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPRSTTSNTLT
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCEE
APYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
CCCHHHHHHHHHHCCCCCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHH
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTL
HHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
SGNPLAMTAGIKTLELLKQEGTYERLESTTERLINGILEAAKAAEVPITGNSIGAMFGFF
CCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LCEGPVRNFEDAKATDAERFGKLHRAMLERGIYLAPSAFEAGFTSLAHSEADIETTLKAF
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHH
RESFAAVA
HHHHHHCC
>Mature Secondary Structure
MNTSRSQAIFSAAQRLMPGGVSSPVRAFRSVGGQPIVFDRVKGAYAWDVDGNRFIDYIGS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHCC
WGPAICGHAHPEVIAALQDALEKGTSFGAPCELENQLAEMVIDAVPSVEMVRFVNSGTEA
CCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
CMSVLRLMRAFTGRDKLIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPRSTTSNTLT
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCEE
APYNDLEAVKELFAENPDAISGVILEPVVGNAGFITPEPGFLEGLRELTREHGALLVFDE
CCCHHHHHHHHHHCCCCCHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHH
VMSGFRISYGGAQARFGVTPDLTTMGKVIGGGLPVGAYGGRAEIMEMVAPAGPMYQAGTL
HHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
SGNPLAMTAGIKTLELLKQEGTYERLESTTERLINGILEAAKAAEVPITGNSIGAMFGFF
CCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LCEGPVRNFEDAKATDAERFGKLHRAMLERGIYLAPSAFEAGFTSLAHSEADIETTLKAF
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHH
RESFAAVA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA