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The map label for this gene is ndhB

Identifier: 124022339

GI number: 124022339

Start: 592154

End: 593725

Strand: Reverse

Name: ndhB

Synonym: P9303_06291

Alternate gene names: 124022339

Gene position: 593725-592154 (Counterclockwise)

Preceding gene: 124022345

Following gene: 124022338

Centisome position: 22.13

GC content: 54.96

Gene sequence:

>1572_bases
ATGCCCGAAATGGGTGTATTTCTTCTCGCCACCCAGGCCATGGCAGCTCCAGGGGAGCTGCTCAACCTTGCACTGAATGC
TGGTGCGATTGCCCCTGAAGGGGCTGTTCTCGTCGCAATGCTGGCGACCCTACTTGTGGACTTGGCCGGTGAGCAGGCTG
CAGCCCGCTGGGTCCCCCCCATTTGTTATGCCGGCCTTGGCACAGCACTCGTGCTCCTCGCCCAGCAATGGAATGCCCCT
CTGGAACCCTCGTTCCTTGGAGCTTTTCTGGCTGACAACCTGGCCATTGCCTTTCGGGCAGTGGTGGCGCTCTCTACCTT
GCTGTCACTACTGATCAGCTGGCGCTACGCGGAACAGAGCGGCACGCCAATTGGTGAATATGCCGCCATCCTTCTGGCCG
CAACCCTCGGGGCAATGCTCCTTTGCGGCTCAACTGATCTTGTGAGCGTGTTCGTCTCCCTCGAGACCCTCTCCGTGGCG
AGCTACCTACTGGCTGGCTACATGAAACGAGATGCCCGTAGTTCCGAAGCAGCACTCAAATATCTGCTGGTGGGCTCCGC
CGCGGCAGCAGTATTTCTCTACGGCGCCTCTCTTCTCTACGGGCTCAGCGGCACTACCAGCTTGCAGGCTATTGGCATTG
CTTTACTCACCAGCCCTACTCCACTGGCCGCACTATCACTGGTCTTCGTACTTGCCACGGTGGCCTTCAAAATTGCTGCG
GTGCCTTTCCACCAATGGACCCCGGATGTCTATGAAGGGTCCCCAACTCCAGTGGTCGCATTCCTCTCAGTGGGGTCTAA
AGCTGCTGGCTTTGCTCTTGCACTGAGGTTGTTGGTTGGTTGCTTCGGTGCCTTCGACAACCAATGGAAATTGCTTTTCA
CCGTGCTGGCTGTGTTGAGCATGACCCTGGGCAACGTTGTCGCGCTGGCGCAGACATCAATGAAACGAATGCTGGCTTAC
AGCTCCATCGGCCAGGCAGGATTCGTGATGATTGGCCTGGTGTGCGGAACCGAAGACGGCTTCGCTGCAATGGTTCTCTA
TATGGCTGCCTATCTATTCATGAACCTGGGAGCCTTTGCCTGCATCATCCTCTTCTCGATCCGCACGGGAAGTGATCGAA
TTTCTGACTATGCAGGTCTCTATCAGAAGGATCCGTTGATAACGCTGGGACTCAGCCTCTGCCTTCTTTCCTTGGGTGGC
ATACCTCCGATGCTCGGATTTTTTGGCAAGATCTATCTTTTCTTTGCTGGCTGGGCCGACCATCAATACCTCCTCGTCGT
TGTTGGCCTAGTGACCTCCGTAGTGTCGATTTATTACTACATCTCTGTTATCAAAATGATGGTGGTCAAAGAACCAAAAG
AAGCTTCCGATGTCGTGAAGTCCTATCCATCGATCCAGTGGTCAACGATTGGAATGCCTCCACTTCGAATAGCACTAGTG
GGTTGCGTTGTTGTAACAGCCGTTGGGGGGATCCTTTCAAATCCTCTCTTTCAATGGGCGAATAATGCCGTAGCCGGCAC
GCCGCTTCTCCAGGAAGCCATAGCTCTAGGCAGCCAACGATCTATTGGCTAA

Upstream 100 bases:

>100_bases
ATCGCACCGGCAAGCAAGCTGCATGATGCGATGTTCTCAATAAGGTATAGCAAGGCAGCACAGCTACAGTCACGCAAATT
GGTCTGATCGTGCACGCGTC

Downstream 100 bases:

>100_bases
TCCAACGGCAAGCAGCACACCCGTCGCCGTGCTGAGCGATGTCAGCAAGCTCTACGGACAAGGAGCTGGAACAGTAAAAG
CTCTAGATCATCTCAATCTG

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPEMGVFLLATQAMAAPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPPICYAGLGTALVLLAQQWNAP
LEPSFLGAFLADNLAIAFRAVVALSTLLSLLISWRYAEQSGTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVA
SYLLAGYMKRDARSSEAALKYLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGIALLTSPTPLAALSLVFVLATVAFKIAA
VPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLSMTLGNVVALAQTSMKRMLAY
SSIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFACIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGG
IPPMLGFFGKIYLFFAGWADHQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIQWSTIGMPPLRIALV
GCVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG

Sequences:

>Translated_523_residues
MPEMGVFLLATQAMAAPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPPICYAGLGTALVLLAQQWNAP
LEPSFLGAFLADNLAIAFRAVVALSTLLSLLISWRYAEQSGTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVA
SYLLAGYMKRDARSSEAALKYLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGIALLTSPTPLAALSLVFVLATVAFKIAA
VPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLSMTLGNVVALAQTSMKRMLAY
SSIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFACIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGG
IPPMLGFFGKIYLFFAGWADHQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIQWSTIGMPPLRIALV
GCVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG
>Mature_522_residues
PEMGVFLLATQAMAAPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPPICYAGLGTALVLLAQQWNAPL
EPSFLGAFLADNLAIAFRAVVALSTLLSLLISWRYAEQSGTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVAS
YLLAGYMKRDARSSEAALKYLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGIALLTSPTPLAALSLVFVLATVAFKIAAV
PFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLSMTLGNVVALAQTSMKRMLAYS
SIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFACIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGGI
PPMLGFFGKIYLFFAGWADHQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIQWSTIGMPPLRIALVG
CVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=318, Percent_Identity=25.1572327044025, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI145693160, Length=372, Percent_Identity=34.4086021505376, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788829, Length=354, Percent_Identity=28.2485875706215, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788831, Length=324, Percent_Identity=25.6172839506173, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788613, Length=357, Percent_Identity=25.7703081232493, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788614, Length=385, Percent_Identity=23.8961038961039, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NU2C_PROM3 (A2C7C1)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016646.1
- STRING:   A2C7C1
- GeneID:   4776314
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_06291
- eggNOG:   COG1007
- HOGENOM:   HBG747830
- OMA:   FAGWADH
- ProtClustDB:   PRK02504
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.6.5.3

Molecular weight: Translated: 54842; Mature: 54710

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1409c1a4)-; HASH(0x137b68f8)-; HASH(0x14a99194)-; HASH(0x150005a8)-; HASH(0x14a98ed0)-; HASH(0x14a98ef4)-; HASH(0x15001c2c)-; HASH(0x14a99158)-; HASH(0x15001d70)-; HASH(0x13f94164)-; HASH(0x15001db8)-; HASH(0x14a966bc)-; HASH(0x130ef30c)-; HASH(0x14a9902c)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEMGVFLLATQAMAAPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPP
CCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCH
ICYAGLGTALVLLAQQWNAPLEPSFLGAFLADNLAIAFRAVVALSTLLSLLISWRYAEQS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVASYLLAGYMKRDARSSEAALK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGIALLTSPTPLAALSLVFVLATVAFKIAA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHH
VPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLS
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MTLGNVVALAQTSMKRMLAYSSIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
CIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGGIPPMLGFFGKIYLFFAGWAD
HHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIQWSTIGMPPLRIALV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCHHHHHHH
GCVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG
HHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCH
>Mature Secondary Structure 
PEMGVFLLATQAMAAPGELLNLALNAGAIAPEGAVLVAMLATLLVDLAGEQAAARWVPP
CCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCH
ICYAGLGTALVLLAQQWNAPLEPSFLGAFLADNLAIAFRAVVALSTLLSLLISWRYAEQS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTPIGEYAAILLAATLGAMLLCGSTDLVSVFVSLETLSVASYLLAGYMKRDARSSEAALK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YLLVGSAAAAVFLYGASLLYGLSGTTSLQAIGIALLTSPTPLAALSLVFVLATVAFKIAA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHH
VPFHQWTPDVYEGSPTPVVAFLSVGSKAAGFALALRLLVGCFGAFDNQWKLLFTVLAVLS
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MTLGNVVALAQTSMKRMLAYSSIGQAGFVMIGLVCGTEDGFAAMVLYMAAYLFMNLGAFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
CIILFSIRTGSDRISDYAGLYQKDPLITLGLSLCLLSLGGIPPMLGFFGKIYLFFAGWAD
HHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
HQYLLVVVGLVTSVVSIYYYISVIKMMVVKEPKEASDVVKSYPSIQWSTIGMPPLRIALV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCHHHHHHH
GCVVVTAVGGILSNPLFQWANNAVAGTPLLQEAIALGSQRSIG
HHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA