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The map label for this gene is pnp [H]

Identifier: 124022312

GI number: 124022312

Start: 568343

End: 570508

Strand: Reverse

Name: pnp [H]

Synonym: P9303_06021

Alternate gene names: 124022312

Gene position: 570508-568343 (Counterclockwise)

Preceding gene: 124022313

Following gene: 124022310

Centisome position: 21.27

GC content: 56.14

Gene sequence:

>2166_bases
GTGCAAGGTCAGACACAGTCGATCTCCTTTGATGGTCGGGAGATTCGGCTGACCACAGGGCGATATGCCCCCCAGGCCGG
TGGTTCCGTGATGATGGAATGCGGCGACACCTCCGTCCTGGTAACCGCCACCAGATCGACAGGCCGCGAAGGCATCGATT
TTCTTCCCCTGATTTGCGACTACGAGGAGCGGCTCTATGCAGCTGGCCGCATTCCTGGCAGTTTCATGCGACGTGAAGGA
CGCCCGCCAGAGAGAGCCACGCTGATCGCTCGTCTAATCGACCGCCCCATGAGGCCCTTATTCCCTAGCTGGATGAGGGA
CGACCTTCAGATCGTGGCAACATGCCTCTCTCTCGATGAACGGGTTCCAGCAGATGTGCTGGCCGTTACGGGAGCTTCCA
TGGCCACCTTGCTAGCAGGCATCCCCTTCCAGGGGCCAATGGCTGCAGTAAGGGTAGGGCTTCTCGGTGATGATTTCGTT
CTGAATCCCAGCTATCGAGAAATCGAACGTGGCGATCTCGATCTCGTGGTAGCTGGCACCCCAGATGGGGTTGTCATGGT
TGAAGCCGGTGCAAACCAGCTGCCAGAAGGGGACGTCATCGAAGCCATCGACTTTGGCTATGAAGCCGTCTGCGAATTAA
TCAAGGCACAGCAATCCATCCTCAAGGATGCAGGGATTAAGCAGGTACAGCCGGAACCCCCCACTCAAGACACCAAGCTT
TCGACTTATCTGGAAAAAAATTGCAGCAAGAGCATCGGGGAAGTGCTGAAGCAATTTGAGCAAACCAAAGCTGAACGCGA
TAGCAAGTTGGATGCCATCAAAGCGAAAACAGCTGAAGCCATTGACAGCCTCAAAGAAGACGATGCCGTTCGCAAGTCAG
TGAATGCCAACAGCAAAGTCTTGAGCAACAACTTCAAGGCGCTCACCAAAAAATTGATGCGTGAGCAGATCATCAAACAA
GGCAAACGCGTCGATGGTCGCAAGCTTGATGAGGTCCGCACCATTACTTCCGCCGCAGGTGTGCTGCCAAAACGCGTGCA
CGGATCCGGACTCTTCCAACGCGGACTCACCCAGGTGCTGTCCACCGCCACCCTGGGCACACCCAGCGATGCCCAGGAGA
TGGATGACCTCAACCCTGGACCTGAGAAGACCTATCTACACCACTACAACTTCCCCCCCTATTCCGTTGGCGAGACACGT
CCAATGCGATCGCCCGGGCGCCGTGAGGTAGGCCATGGTTCCCTCGCTGAACGCGCCATCATTCCAGTACTGCCTCCCAA
GGACACCTTCCCTTACGTGCTCAGGGTGGTGAGCGAAGTCCTCAGCTCCAATGGGTCCACCTCGATGGGCTCGGTCTGCG
GCAGCACCCTGGCCCTCATGGATGCAGGTGTTCCACTGAAGGCTCCAGTCAGCGGAGCCGCCATGGGCTTGATCAAGGAA
GATGCTGAGATTCGCATCCTCACTGACATCCAGGGAATCGAGGACTTCCTCGGTGATATGGACTTCAAAGTGGCTGGAAC
AAAAGATGGCATCACAGCCCTGCAGATGGACATGAAGATCACCGGCCTTCCTGTAAAAACCATTGCAGAAGCTGTCAATC
AGGCCCGCCCTGCCAGGATCCATATCCTCGAGAAGATGCTGGAAGCCATTGACGCTCCCAGGACTAGCCTCTCTCCCCAT
GCCCCGCGCCTGCTGAGCTTCAGAATCGATCCAGAACTGATCGGCACAGTGATTGGACCGGGTGGTCGCACAATCAAGGG
CATCACAGAACGCACGAACACCAAGATCGACATTGAAGACGGTGGCATCGTCACGATTGCTTCTCATGACGGGGCTGCTG
CAGAGGCAGCCCAACGCATCATCGAAGGACTCACCCGCAAAGTAAACGAGGGGGAAGTGTTCAGCGGAACCATCACAAGG
ATCATTCCGATCGGGGCTTTCGTAGAGATCCTGCCCGGCAAAGAGGGCATGATCCACATCTCCCAGCTGTCAGAGGCTCG
CGTGGAGAAGGTCGACGACGTCGTGAAAGTAGGTGATGAGGTGACAGTACGAATACGAGAGATTGACAATCGTGGCCGTA
TCAACCTCACACTCAGAGGCGTCCCTCAGAACGGCGAAGAGACTCAATCAGAGCCAGCTCCAACTCCTGTAGCCCCTCTC
AACTGA

Upstream 100 bases:

>100_bases
TGCTTAGCAACTGAACCAACAGGAATCAATTCCAAAATTCCACGAAAGTTGACTCGGCAAAGTGCCAGTATGGTGCTTGA
CAAAAAAAACATAAGAGAAC

Downstream 100 bases:

>100_bases
GGGGGTACAGGTCAATTCAAACCTGAAAGCCTGGGTCGATCTCTTCCATAGCCCGCGCCGCTGCTTCACAAAGCTGGGCG
TGGGCGAGGCCATGGCTGGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MQGQTQSISFDGREIRLTTGRYAPQAGGSVMMECGDTSVLVTATRSTGREGIDFLPLICDYEERLYAAGRIPGSFMRREG
RPPERATLIARLIDRPMRPLFPSWMRDDLQIVATCLSLDERVPADVLAVTGASMATLLAGIPFQGPMAAVRVGLLGDDFV
LNPSYREIERGDLDLVVAGTPDGVVMVEAGANQLPEGDVIEAIDFGYEAVCELIKAQQSILKDAGIKQVQPEPPTQDTKL
STYLEKNCSKSIGEVLKQFEQTKAERDSKLDAIKAKTAEAIDSLKEDDAVRKSVNANSKVLSNNFKALTKKLMREQIIKQ
GKRVDGRKLDEVRTITSAAGVLPKRVHGSGLFQRGLTQVLSTATLGTPSDAQEMDDLNPGPEKTYLHHYNFPPYSVGETR
PMRSPGRREVGHGSLAERAIIPVLPPKDTFPYVLRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPLKAPVSGAAMGLIKE
DAEIRILTDIQGIEDFLGDMDFKVAGTKDGITALQMDMKITGLPVKTIAEAVNQARPARIHILEKMLEAIDAPRTSLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEAAQRIIEGLTRKVNEGEVFSGTITR
IIPIGAFVEILPGKEGMIHISQLSEARVEKVDDVVKVGDEVTVRIREIDNRGRINLTLRGVPQNGEETQSEPAPTPVAPL
N

Sequences:

>Translated_721_residues
MQGQTQSISFDGREIRLTTGRYAPQAGGSVMMECGDTSVLVTATRSTGREGIDFLPLICDYEERLYAAGRIPGSFMRREG
RPPERATLIARLIDRPMRPLFPSWMRDDLQIVATCLSLDERVPADVLAVTGASMATLLAGIPFQGPMAAVRVGLLGDDFV
LNPSYREIERGDLDLVVAGTPDGVVMVEAGANQLPEGDVIEAIDFGYEAVCELIKAQQSILKDAGIKQVQPEPPTQDTKL
STYLEKNCSKSIGEVLKQFEQTKAERDSKLDAIKAKTAEAIDSLKEDDAVRKSVNANSKVLSNNFKALTKKLMREQIIKQ
GKRVDGRKLDEVRTITSAAGVLPKRVHGSGLFQRGLTQVLSTATLGTPSDAQEMDDLNPGPEKTYLHHYNFPPYSVGETR
PMRSPGRREVGHGSLAERAIIPVLPPKDTFPYVLRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPLKAPVSGAAMGLIKE
DAEIRILTDIQGIEDFLGDMDFKVAGTKDGITALQMDMKITGLPVKTIAEAVNQARPARIHILEKMLEAIDAPRTSLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEAAQRIIEGLTRKVNEGEVFSGTITR
IIPIGAFVEILPGKEGMIHISQLSEARVEKVDDVVKVGDEVTVRIREIDNRGRINLTLRGVPQNGEETQSEPAPTPVAPL
N
>Mature_721_residues
MQGQTQSISFDGREIRLTTGRYAPQAGGSVMMECGDTSVLVTATRSTGREGIDFLPLICDYEERLYAAGRIPGSFMRREG
RPPERATLIARLIDRPMRPLFPSWMRDDLQIVATCLSLDERVPADVLAVTGASMATLLAGIPFQGPMAAVRVGLLGDDFV
LNPSYREIERGDLDLVVAGTPDGVVMVEAGANQLPEGDVIEAIDFGYEAVCELIKAQQSILKDAGIKQVQPEPPTQDTKL
STYLEKNCSKSIGEVLKQFEQTKAERDSKLDAIKAKTAEAIDSLKEDDAVRKSVNANSKVLSNNFKALTKKLMREQIIKQ
GKRVDGRKLDEVRTITSAAGVLPKRVHGSGLFQRGLTQVLSTATLGTPSDAQEMDDLNPGPEKTYLHHYNFPPYSVGETR
PMRSPGRREVGHGSLAERAIIPVLPPKDTFPYVLRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPLKAPVSGAAMGLIKE
DAEIRILTDIQGIEDFLGDMDFKVAGTKDGITALQMDMKITGLPVKTIAEAVNQARPARIHILEKMLEAIDAPRTSLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEAAQRIIEGLTRKVNEGEVFSGTITR
IIPIGAFVEILPGKEGMIHISQLSEARVEKVDDVVKVGDEVTVRIREIDNRGRINLTLRGVPQNGEETQSEPAPTPVAPL
N

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=737, Percent_Identity=36.3636363636364, Blast_Score=431, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=699, Percent_Identity=47.4964234620887, Blast_Score=619, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI115534063, Length=705, Percent_Identity=34.468085106383, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI281362905, Length=697, Percent_Identity=36.7288378766141, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24651641, Length=697, Percent_Identity=36.7288378766141, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24651643, Length=697, Percent_Identity=36.7288378766141, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI161079377, Length=641, Percent_Identity=36.6614664586583, Blast_Score=397, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77899; Mature: 77899

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGQTQSISFDGREIRLTTGRYAPQAGGSVMMECGDTSVLVTATRSTGREGIDFLPLICD
CCCCCCEEECCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCHHHHEEC
YEERLYAAGRIPGSFMRREGRPPERATLIARLIDRPMRPLFPSWMRDDLQIVATCLSLDE
HHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
RVPADVLAVTGASMATLLAGIPFQGPMAAVRVGLLGDDFVLNPSYREIERGDLDLVVAGT
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEECCCCEEECCCHHHHCCCCEEEEEECC
PDGVVMVEAGANQLPEGDVIEAIDFGYEAVCELIKAQQSILKDAGIKQVQPEPPTQDTKL
CCCEEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
STYLEKNCSKSIGEVLKQFEQTKAERDSKLDAIKAKTAEAIDSLKEDDAVRKSVNANSKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSNNFKALTKKLMREQIIKQGKRVDGRKLDEVRTITSAAGVLPKRVHGSGLFQRGLTQVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHH
STATLGTPSDAQEMDDLNPGPEKTYLHHYNFPPYSVGETRPMRSPGRREVGHGSLAERAI
HHHCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCE
IPVLPPKDTFPYVLRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPLKAPVSGAAMGLIKE
EECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEC
DAEIRILTDIQGIEDFLGDMDFKVAGTKDGITALQMDMKITGLPVKTIAEAVNQARPARI
CCCEEEEECHHHHHHHHCCCCEEEECCCCCCEEEEECCEEECCCHHHHHHHHHHCCCHHH
HILEKMLEAIDAPRTSLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEAAQRIIEGLTRKVNEGEVFSGTITRIIPIGAFVEILPGKEGMIHI
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHEECCCCCCEEEH
SQLSEARVEKVDDVVKVGDEVTVRIREIDNRGRINLTLRGVPQNGEETQSEPAPTPVAPL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
N
C
>Mature Secondary Structure
MQGQTQSISFDGREIRLTTGRYAPQAGGSVMMECGDTSVLVTATRSTGREGIDFLPLICD
CCCCCCEEECCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCHHHHEEC
YEERLYAAGRIPGSFMRREGRPPERATLIARLIDRPMRPLFPSWMRDDLQIVATCLSLDE
HHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
RVPADVLAVTGASMATLLAGIPFQGPMAAVRVGLLGDDFVLNPSYREIERGDLDLVVAGT
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEECCCCEEECCCHHHHCCCCEEEEEECC
PDGVVMVEAGANQLPEGDVIEAIDFGYEAVCELIKAQQSILKDAGIKQVQPEPPTQDTKL
CCCEEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
STYLEKNCSKSIGEVLKQFEQTKAERDSKLDAIKAKTAEAIDSLKEDDAVRKSVNANSKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSNNFKALTKKLMREQIIKQGKRVDGRKLDEVRTITSAAGVLPKRVHGSGLFQRGLTQVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHH
STATLGTPSDAQEMDDLNPGPEKTYLHHYNFPPYSVGETRPMRSPGRREVGHGSLAERAI
HHHCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCE
IPVLPPKDTFPYVLRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPLKAPVSGAAMGLIKE
EECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEC
DAEIRILTDIQGIEDFLGDMDFKVAGTKDGITALQMDMKITGLPVKTIAEAVNQARPARI
CCCEEEEECHHHHHHHHCCCCEEEECCCCCCEEEEECCEEECCCHHHHHHHHHHCCCHHH
HILEKMLEAIDAPRTSLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEAAQRIIEGLTRKVNEGEVFSGTITRIIPIGAFVEILPGKEGMIHI
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHEECCCCCCEEEH
SQLSEARVEKVDDVVKVGDEVTVRIREIDNRGRINLTLRGVPQNGEETQSEPAPTPVAPL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]