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The map label for this gene is gidB

Identifier: 124022160

GI number: 124022160

Start: 452803

End: 453546

Strand: Reverse

Name: gidB

Synonym: P9303_04501

Alternate gene names: 124022160

Gene position: 453546-452803 (Counterclockwise)

Preceding gene: 124022163

Following gene: 124022157

Centisome position: 16.91

GC content: 58.06

Gene sequence:

>744_bases
ATGTCAAATCAGGCCTGCTTTAGCAACCCAGGCCCGGAGCTCTGGAATGCCCTTGGATGGCATCCCTCCAGTGAACAACT
GGAGCAAATGATCGCCCTACAAGCCCTCCTTCGCCAATGGAATGCCCGGGTCAACCTCACCCGCCTGGTCGAAGGTGGTG
ACTACTGGATTTTGCAGGTGTTCGACAGCCTCTGGCCTCTGCAGAGCGAATTGCAGAACGCCCAACAACCACGTCGTTGC
ATCGATATCGGCAGCGGTGGTGGCTTTCCCGGTCTGGTGCTAGCCATCGCACTACCAGGCGCAAGTATCACCTTGGTGGA
TTCGGTAGGCCGCAAGACAGCTGCCCTAAAGGCAATGGCGGCAAAGCTAGGGCTAACATCCCGCGTCACGGTACGTAGCG
AACGGGCAGAACTCACCGGTCAGGATCACTGCTGCCGCGGCTTGTTTGATCTCGCGATGGCTCGCGCAGTGAGCACAGCA
CCGGTTGTGGCCGAATACCTCGTCCCCCTTCTCAAGCCAAGCGGTGAAGCCCTGCTATTTCGTGGCCATTGGAGCCCCAA
CGATGCAAAGGATCTCGCTAAGGCTCTCAGATTTCTACAAGCCGATCTAATCAAGATGGAACGTCGCGAACTTCCTGACA
ATCGCGGGGTGCGTCACCAATTACGCCTGCGCGCAACGCTCCCCTGCCCAGCGACCTTTCCACGTCCAATCGGTGTACCT
GCCAAGAACCCGCTCGGGTCTTAA

Upstream 100 bases:

>100_bases
CGCAAGAGTCAAAACCAGAAGTTGATCGGCAACTCGCAGAAAATCATCTCCTTTCCCCTTGAACAATTCCATCCTAGAGA
GAACTATGTTCTGATTGCCC

Downstream 100 bases:

>100_bases
ACACTGGTCAGTCGTTGCTGAGGATGACCACCAAGACGGTTGCGCAACCTACGCAGCAAGCCTGGGATCTGATCACACCA
GGGGCTGTTCACCAACACCT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWILQVFDSLWPLQSELQNAQQPRRC
IDIGSGGGFPGLVLAIALPGASITLVDSVGRKTAALKAMAAKLGLTSRVTVRSERAELTGQDHCCRGLFDLAMARAVSTA
PVVAEYLVPLLKPSGEALLFRGHWSPNDAKDLAKALRFLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVP
AKNPLGS

Sequences:

>Translated_247_residues
MSNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWILQVFDSLWPLQSELQNAQQPRRC
IDIGSGGGFPGLVLAIALPGASITLVDSVGRKTAALKAMAAKLGLTSRVTVRSERAELTGQDHCCRGLFDLAMARAVSTA
PVVAEYLVPLLKPSGEALLFRGHWSPNDAKDLAKALRFLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVP
AKNPLGS
>Mature_246_residues
SNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWILQVFDSLWPLQSELQNAQQPRRCI
DIGSGGGFPGLVLAIALPGASITLVDSVGRKTAALKAMAAKLGLTSRVTVRSERAELTGQDHCCRGLFDLAMARAVSTAP
VVAEYLVPLLKPSGEALLFRGHWSPNDAKDLAKALRFLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVPA
KNPLGS

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=137, Percent_Identity=29.1970802919708, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_PROM3 (A2C6U2)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016467.1
- ProteinModelPortal:   A2C6U2
- STRING:   A2C6U2
- GeneID:   4777551
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_04501
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   AITHPNS
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27031; Mature: 26899

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWILQV
CCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHH
FDSLWPLQSELQNAQQPRRCIDIGSGGGFPGLVLAIALPGASITLVDSVGRKTAALKAMA
HHHHCCHHHHHHHHHCCHHHEEECCCCCHHHHHHHHCCCCCCEEEEHHHCHHHHHHHHHH
AKLGLTSRVTVRSERAELTGQDHCCRGLFDLAMARAVSTAPVVAEYLVPLLKPSGEALLF
HHHCCCCEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
RGHWSPNDAKDLAKALRFLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVP
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCC
AKNPLGS
CCCCCCC
>Mature Secondary Structure 
SNQACFSNPGPELWNALGWHPSSEQLEQMIALQALLRQWNARVNLTRLVEGGDYWILQV
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHH
FDSLWPLQSELQNAQQPRRCIDIGSGGGFPGLVLAIALPGASITLVDSVGRKTAALKAMA
HHHHCCHHHHHHHHHCCHHHEEECCCCCHHHHHHHHCCCCCCEEEEHHHCHHHHHHHHHH
AKLGLTSRVTVRSERAELTGQDHCCRGLFDLAMARAVSTAPVVAEYLVPLLKPSGEALLF
HHHCCCCEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
RGHWSPNDAKDLAKALRFLQADLIKMERRELPDNRGVRHQLRLRATLPCPATFPRPIGVP
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCC
AKNPLGS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA