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The map label for this gene is hemB [H]

Identifier: 124022098

GI number: 124022098

Start: 390435

End: 391436

Strand: Direct

Name: hemB [H]

Synonym: P9303_03881

Alternate gene names: 124022098

Gene position: 390435-391436 (Clockwise)

Preceding gene: 124022097

Following gene: 124022099

Centisome position: 14.55

GC content: 54.99

Gene sequence:

>1002_bases
ATGGATCTCACTTATCGCCCTCGTCGGCTGCGTCGTACTCCTGCCCTGCGTTCCATGGTGCGGGAGCACAGCCTCACTGC
TGCTGACTTTATCTATCCCTTGTTTGTCCATGAGGGAGCTGATGTGGAGCCGATCGGTGCCATGCCAGGAACCAATCGCT
GGAGCCTGGATCGCTTAATGGGAGAAGTTCAACGGGCCTGGGGTCTGGGCATTCGTTGTGTGGTGCTTTTCCCGAAAGTC
GCTGAGGGCCTCAAAACTGAAGATGGGGCGGAATGTTTCAGTGAGCATGGGTTGATTCCGCGGGCCATCACGCAGCTCAA
GCATGAGCTGCCGGAAATGACAGTGATGACCGATGTGGCATTGGATCCCTATTCCTGTGATGGTCATGACGGCATCGTGA
GCGCCGAGGGTGTGGTGCTGAACGACGAGACGATTGAACAACTCTGTCGTCAGGCTGTTGTTCAGGCTCGTGCTGGTGCT
GACCTGATTGGACCCAGCGACATGATGGATGGTCGCGTCGGTGCGATCCGTGAAGCTCTTGACGACGAGGGTTTTGAGCA
TGTGGGGATTATTAGCTATACAGCGAAATACTCTTCGGCGTACTACGGCCCGTTTCGCGAGGCGCTTGATTCAGCCCCGC
GCGTGGCTGGTGGCAAGCCAATTCCTAAAGACAAGAGCACGTATCAAATGGATCCAGCCAATGCTCGTGAGGCGATTACT
GAGGCTCAACTCGATGAGCAGGAGGGTGCAGATATTCTCATGGTGAAGCCTGGCTTGGCCTATCTCGACATCATTCATCG
TTTAAGCGAAGAATCGGAGTTACCCATCGCTGCTTACAACGTGAGTGGCGAGTATTCGATGGTGAAGGCTGCTGCTGAAA
GGGGTTGGCTTGATGAGCGGGCGGTGGTCTTAGAAACCTTGTTGAGTTTCAAGCGAGCTGGTGCCGATTTGATCCTCACC
TACCACGCTTGTGATGCAGCCGCTTGGTTACGGCAGGGATGA

Upstream 100 bases:

>100_bases
GGTTGCAGCAGTCCCGCAGTACTGACCCCCGTCAGGCGTGGCTTCAGATGGCTCCACTCTGAGGCGAGACTTACGCTCAC
GAGATTGTTGTGCGGTTTTG

Downstream 100 bases:

>100_bases
TTGGCTTCTTTCAAGTTCAGTGGGGCGCAACAGCGTTGAAATGAGCGCTGTAGTCATGAGCTCTGAGGTCTCGACTGCTT
CGCTTGCCTTTCAGGACACA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDLTYRPRRLRRTPALRSMVREHSLTAADFIYPLFVHEGADVEPIGAMPGTNRWSLDRLMGEVQRAWGLGIRCVVLFPKV
AEGLKTEDGAECFSEHGLIPRAITQLKHELPEMTVMTDVALDPYSCDGHDGIVSAEGVVLNDETIEQLCRQAVVQARAGA
DLIGPSDMMDGRVGAIREALDDEGFEHVGIISYTAKYSSAYYGPFREALDSAPRVAGGKPIPKDKSTYQMDPANAREAIT
EAQLDEQEGADILMVKPGLAYLDIIHRLSEESELPIAAYNVSGEYSMVKAAAERGWLDERAVVLETLLSFKRAGADLILT
YHACDAAAWLRQG

Sequences:

>Translated_333_residues
MDLTYRPRRLRRTPALRSMVREHSLTAADFIYPLFVHEGADVEPIGAMPGTNRWSLDRLMGEVQRAWGLGIRCVVLFPKV
AEGLKTEDGAECFSEHGLIPRAITQLKHELPEMTVMTDVALDPYSCDGHDGIVSAEGVVLNDETIEQLCRQAVVQARAGA
DLIGPSDMMDGRVGAIREALDDEGFEHVGIISYTAKYSSAYYGPFREALDSAPRVAGGKPIPKDKSTYQMDPANAREAIT
EAQLDEQEGADILMVKPGLAYLDIIHRLSEESELPIAAYNVSGEYSMVKAAAERGWLDERAVVLETLLSFKRAGADLILT
YHACDAAAWLRQG
>Mature_333_residues
MDLTYRPRRLRRTPALRSMVREHSLTAADFIYPLFVHEGADVEPIGAMPGTNRWSLDRLMGEVQRAWGLGIRCVVLFPKV
AEGLKTEDGAECFSEHGLIPRAITQLKHELPEMTVMTDVALDPYSCDGHDGIVSAEGVVLNDETIEQLCRQAVVQARAGA
DLIGPSDMMDGRVGAIREALDDEGFEHVGIISYTAKYSSAYYGPFREALDSAPRVAGGKPIPKDKSTYQMDPANAREAIT
EAQLDEQEGADILMVKPGLAYLDIIHRLSEESELPIAAYNVSGEYSMVKAAAERGWLDERAVVLETLLSFKRAGADLILT
YHACDAAAWLRQG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=312, Percent_Identity=39.1025641025641, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI87081728, Length=330, Percent_Identity=48.7878787878788, Blast_Score=278, Evalue=5e-76,
Organism=Saccharomyces cerevisiae, GI6321398, Length=321, Percent_Identity=36.7601246105919, Blast_Score=192, Evalue=6e-50,
Organism=Drosophila melanogaster, GI21358291, Length=298, Percent_Identity=38.5906040268456, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36594; Mature: 36594

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTYRPRRLRRTPALRSMVREHSLTAADFIYPLFVHEGADVEPIGAMPGTNRWSLDRLM
CCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
GEVQRAWGLGIRCVVLFPKVAEGLKTEDGAECFSEHGLIPRAITQLKHELPEMTVMTDVA
HHHHHHHCCCEEEEEEEHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEHHHCC
LDPYSCDGHDGIVSAEGVVLNDETIEQLCRQAVVQARAGADLIGPSDMMDGRVGAIREAL
CCCCCCCCCCCEEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHH
DDEGFEHVGIISYTAKYSSAYYGPFREALDSAPRVAGGKPIPKDKSTYQMDPANAREAIT
CCCCCCEEEEEEEEHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHH
EAQLDEQEGADILMVKPGLAYLDIIHRLSEESELPIAAYNVSGEYSMVKAAAERGWLDER
HHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHH
AVVLETLLSFKRAGADLILTYHACDAAAWLRQG
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC
>Mature Secondary Structure
MDLTYRPRRLRRTPALRSMVREHSLTAADFIYPLFVHEGADVEPIGAMPGTNRWSLDRLM
CCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
GEVQRAWGLGIRCVVLFPKVAEGLKTEDGAECFSEHGLIPRAITQLKHELPEMTVMTDVA
HHHHHHHCCCEEEEEEEHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEHHHCC
LDPYSCDGHDGIVSAEGVVLNDETIEQLCRQAVVQARAGADLIGPSDMMDGRVGAIREAL
CCCCCCCCCCCEEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHH
DDEGFEHVGIISYTAKYSSAYYGPFREALDSAPRVAGGKPIPKDKSTYQMDPANAREAIT
CCCCCCEEEEEEEEHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHH
EAQLDEQEGADILMVKPGLAYLDIIHRLSEESELPIAAYNVSGEYSMVKAAAERGWLDER
HHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHH
AVVLETLLSFKRAGADLILTYHACDAAAWLRQG
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626508 [H]