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The map label for this gene is rpoD2 [H]

Identifier: 124021877

GI number: 124021877

Start: 181490

End: 182542

Strand: Direct

Name: rpoD2 [H]

Synonym: P9303_01641

Alternate gene names: 124021877

Gene position: 181490-182542 (Clockwise)

Preceding gene: 124021876

Following gene: 124021881

Centisome position: 6.77

GC content: 54.8

Gene sequence:

>1053_bases
TTGGACTGCTCGGGAGTAAGCTTTACAAAACGCAATAGAGTTGTCGTGGTCGACTCCGCAGTCTCCAAAGCCCTTGTTAA
ATCAGCGGTTGTGCCTGCTCGTCAGTTGCCCGCAGATGTCGACCTGGTGCGTTCATACCTGCGCGATATCGGTCGAGTGC
CGCTACTGAGCCATGAGCAGGAGATCACGCTGGGTCGTCAGGTGCAGGAGTTGATGTCTTTAGAGCAGCTTGAGTCTGAA
CTGGAAGGTAAAACAGGCGCGCCAGCGAGTCGTAAAGAACTAGCGAAGGCAGCTGGATTGAGTGAGTTGCAGCTCAAGAA
GAAGTTGCAGATCGGACGACGTGCGAAGGAGCGGATGGTGTCGGCGAACCTGCGCTTAGTGGTGAGTGTTGCCAAGAAGT
ACACCAAAAGGAATATGGAGCTTCTTGATTTGATCCAAGAGGGAACGATCGGCTTGGTGAGGGGAGTGGAGAAGTTCGAC
CCAACCCGTGGCTACAAGTTTTCGACCTATGCGTATTGGTGGATTCGCCAGGGGATCACGCGTGCGATTGCGGAGAAGAG
CCGGACGATCCGTCTGCCGATCCACATCACAGAGATGCTGAACAAGCTCAAGAAAGGCCAGCGAGAGTTAAGTCAGGAGA
TGGGGCGCACGCCAACAGTGAGCGAACTTGCAGAGTTTGTGGAGTTGCCCGAGGAGGAGGTGAAGGATCTGATGTGCCGT
GCACGTCAGCCGATGAGTTTGGAGATGAAGGTGGGAGATGGGGATGAAACGGAGTTGCTTGAGTTGCTTGCCGGGGAAGA
GGAGTTACCGAGTGAGAAGGTGGAAGTGGATTGCATGAAAGGCGATTTACGTACCTTGCTGGAAAAGTTGCCCGAGTTGC
AGGGTCGTGTGCTGCGGATGCGTTATGGAATCGACGGAGGCGAGCCGATGAACCTCACCGGGATTGGTCGCATCCTCGAC
ATCAGTCGTGACCGTGTTCGCAATCTGGAGCGCCATGGACTCAATGGTCTGCGCCAGTTGAGTGAAACGGTTGAGGCCTA
TGCGGCTTGCTGA

Upstream 100 bases:

>100_bases
ATTTACGGACAGCGGCTTGGCTGCTTGAGCAAACAAATGGCATAGCGTTCTAGGTATTTATGCCTAAACCGCCTGGGGAG
GGGGTTTCCCGAAGGGCTGT

Downstream 100 bases:

>100_bases
ATGATTCTGAGCAGCAGCGGATTGACTTCTTCAGGCGCTTCGTCGTGTGGGCAATGCCCTGCACCATTGACCACTGATAG
GGAGCGGATACAAGGCAGCA

Product: type II alternative RNA polymerase sigma factor, sigma-70 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDCSGVSFTKRNRVVVVDSAVSKALVKSAVVPARQLPADVDLVRSYLRDIGRVPLLSHEQEITLGRQVQELMSLEQLESE
LEGKTGAPASRKELAKAAGLSELQLKKKLQIGRRAKERMVSANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFD
PTRGYKFSTYAYWWIRQGITRAIAEKSRTIRLPIHITEMLNKLKKGQRELSQEMGRTPTVSELAEFVELPEEEVKDLMCR
ARQPMSLEMKVGDGDETELLELLAGEEELPSEKVEVDCMKGDLRTLLEKLPELQGRVLRMRYGIDGGEPMNLTGIGRILD
ISRDRVRNLERHGLNGLRQLSETVEAYAAC

Sequences:

>Translated_350_residues
MDCSGVSFTKRNRVVVVDSAVSKALVKSAVVPARQLPADVDLVRSYLRDIGRVPLLSHEQEITLGRQVQELMSLEQLESE
LEGKTGAPASRKELAKAAGLSELQLKKKLQIGRRAKERMVSANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFD
PTRGYKFSTYAYWWIRQGITRAIAEKSRTIRLPIHITEMLNKLKKGQRELSQEMGRTPTVSELAEFVELPEEEVKDLMCR
ARQPMSLEMKVGDGDETELLELLAGEEELPSEKVEVDCMKGDLRTLLEKLPELQGRVLRMRYGIDGGEPMNLTGIGRILD
ISRDRVRNLERHGLNGLRQLSETVEAYAAC
>Mature_350_residues
MDCSGVSFTKRNRVVVVDSAVSKALVKSAVVPARQLPADVDLVRSYLRDIGRVPLLSHEQEITLGRQVQELMSLEQLESE
LEGKTGAPASRKELAKAAGLSELQLKKKLQIGRRAKERMVSANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFD
PTRGYKFSTYAYWWIRQGITRAIAEKSRTIRLPIHITEMLNKLKKGQRELSQEMGRTPTVSELAEFVELPEEEVKDLMCR
ARQPMSLEMKVGDGDETELLELLAGEEELPSEKVEVDCMKGDLRTLLEKLPELQGRVLRMRYGIDGGEPMNLTGIGRILD
ISRDRVRNLERHGLNGLRQLSETVEAYAAC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is a component of the biological clock pathway that affects the circadian expression of a subset of ge

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=258, Percent_Identity=43.4108527131783, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1789098, Length=305, Percent_Identity=37.0491803278689, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=24.3801652892562, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=27.4509803921569, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 39426; Mature: 39426

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCSGVSFTKRNRVVVVDSAVSKALVKSAVVPARQLPADVDLVRSYLRDIGRVPLLSHEQ
CCCCCCCEECCCCEEEEEHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCCCCCH
EITLGRQVQELMSLEQLESELEGKTGAPASRKELAKAAGLSELQLKKKLQIGRRAKERMV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFDPTRGYKFSTYAYWWIRQGIT
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RAIAEKSRTIRLPIHITEMLNKLKKGQRELSQEMGRTPTVSELAEFVELPEEEVKDLMCR
HHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
ARQPMSLEMKVGDGDETELLELLAGEEELPSEKVEVDCMKGDLRTLLEKLPELQGRVLRM
CCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
RYGIDGGEPMNLTGIGRILDISRDRVRNLERHGLNGLRQLSETVEAYAAC
HCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDCSGVSFTKRNRVVVVDSAVSKALVKSAVVPARQLPADVDLVRSYLRDIGRVPLLSHEQ
CCCCCCCEECCCCEEEEEHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCCCCCCH
EITLGRQVQELMSLEQLESELEGKTGAPASRKELAKAAGLSELQLKKKLQIGRRAKERMV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFDPTRGYKFSTYAYWWIRQGIT
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RAIAEKSRTIRLPIHITEMLNKLKKGQRELSQEMGRTPTVSELAEFVELPEEEVKDLMCR
HHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
ARQPMSLEMKVGDGDETELLELLAGEEELPSEKVEVDCMKGDLRTLLEKLPELQGRVLRM
CCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
RYGIDGGEPMNLTGIGRILDISRDRVRNLERHGLNGLRQLSETVEAYAAC
HCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8665856; 1368828 [H]