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The map label for this gene is sqdB [C]

Identifier: 124021766

GI number: 124021766

Start: 51843

End: 53039

Strand: Reverse

Name: sqdB [C]

Synonym: P9303_00511

Alternate gene names: 124021766

Gene position: 53039-51843 (Counterclockwise)

Preceding gene: 124021767

Following gene: 124021765

Centisome position: 1.98

GC content: 54.72

Gene sequence:

>1197_bases
TTGAAAGTTTTCGTTCTCGGCGGCGACGGCTTCTGCGGATGGCCTTGTGCAGTGAACCTCGCTGATAGGGGTCACGAAGT
GTTGATCGTCGACAATCTCAGCCGCCGCAAGATCGATATCGATCTTGAAGTCGAGTCACTGACACCAATCACCAGCATTG
GTGACCGCCTCAGGGCCTGGCGAGAAATCGGAGGCAAGCCAATTCGTTTTGAGCACCTAGACATCGCCCATCAGTACGAC
CGATTGGTGACAATGCTCAAAACTGAACGACCCGATGCGGTGGTTCACTTTGCCGAGCAGCGTGCAGCTCCCTACTCGAT
GAAGAGCAGCTCCACCAAGCGCTATACCGTCGATAACAACGTCAACGGCACTCACAATCTGCTCGCCGCCATCGTTGAAA
GCGGCCTCGACATCCACATCGTGCACCTGGGAACGATGGGGGTGTACGGCTACGGCTCGCACCGCGGAGCCACCATTCCT
GAGGGATACCTCAAAGTGGAAGTGCCCCAGCCCGATGGCAGCCGTTTCGAGGAAGAAATTCTTCACCCAGCCAGCCCTGG
AAGCGTCTATCACATGACCAAGACGCTCGATCAACTGCTTTTCCTCTACTACAACAAGAATGACCAAATCAGGATCACCG
ACCTTCACCAAGGCATCGTCTGGGGTACCAACACTGAAGCAACCGCTAGGGATCCACGCCTAACCAACCGCTTTGATTAC
GACGGTGACTACGGCACAGTTCTCAACCGCTTCCTGATGCAGGCAGCAATCGGTTATCCGCTCACTGTCCATGGAACAGG
AGGTCAAACCCGTGCCTTCATCCACATCTGCGATTCTGTGAAGTGCGTACAACTGGCTCTTGAAAACCCACCGGCCAAAG
GCGAACGAGTCAAGATTTTCAACCAAATGACCGAAAGCCACCAAGTGGGTGAACTTGCCAAGAAGGTAGCTGCCCTAACC
GGCGCCGAACTAAACCATCTTCCAAACCCACGCAACGAAGCAGTGGAGAATGATCTGATTGTGGATAACCGCTGCTTCAT
CGAACTTGGACTCAAACCAACCACCCTCGACGATGGCCTACTTGCTGAAGTAGTCGATGTGGCAAAACGCTGGGCAGACC
GCTGCGACCGCAGTCGTATCCCATGTGTATCGGCCTGGACTTCGACCCAGGCTGAGGCCATCAAGCACCCCTCCTAA

Upstream 100 bases:

>100_bases
CTTGTGACATGCCTAATCATCGTCAGGATCGCCATCAGCTGAAAACCTTGTAGTAGCTTGCCCTGACTGAGCGAATTGCT
CGTTCCTGCAGGAGTTTGAG

Downstream 100 bases:

>100_bases
ACAGCCTGCCCACACCACCCGCTGAACCAGTGAAGATCGCCTTTTTCACAGAGACCTTCCTCCCAAAAGTGGATGGAATC
GTGACACGACTCACCAAAAC

Product: sulfolipid (UDP-sulfoquinovose) biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Nucleoside-Diphosphate-Sugar Epimerase; Epimerase; UDP Sulfoquinovose Synthase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKVFVLGGDGFCGWPCAVNLADRGHEVLIVDNLSRRKIDIDLEVESLTPITSIGDRLRAWREIGGKPIRFEHLDIAHQYD
RLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNNVNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIFNQMTESHQVGELAKKVAALT
GAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGLLAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKHPS

Sequences:

>Translated_398_residues
MKVFVLGGDGFCGWPCAVNLADRGHEVLIVDNLSRRKIDIDLEVESLTPITSIGDRLRAWREIGGKPIRFEHLDIAHQYD
RLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNNVNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIFNQMTESHQVGELAKKVAALT
GAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGLLAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKHPS
>Mature_398_residues
MKVFVLGGDGFCGWPCAVNLADRGHEVLIVDNLSRRKIDIDLEVESLTPITSIGDRLRAWREIGGKPIRFEHLDIAHQYD
RLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNNVNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIFNQMTESHQVGELAKKVAALT
GAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGLLAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKHPS

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 44317; Mature: 44317

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFVLGGDGFCGWPCAVNLADRGHEVLIVDNLSRRKIDIDLEVESLTPITSIGDRLRAW
CEEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
REIGGKPIRFEHLDIAHQYDRLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNN
HHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHEEEHHHCCCCCCCCCCCCEEEEECCC
VNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCHHHHHHHHHHHCCCEEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCEEHHH
NQMTESHQVGELAKKVAALTGAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGL
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCCEEECCEEEEEECCCCCCCCCHH
LAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKHPS
HHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCC
>Mature Secondary Structure
MKVFVLGGDGFCGWPCAVNLADRGHEVLIVDNLSRRKIDIDLEVESLTPITSIGDRLRAW
CEEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
REIGGKPIRFEHLDIAHQYDRLVTMLKTERPDAVVHFAEQRAAPYSMKSSSTKRYTVDNN
HHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHEEEHHHCCCCCCCCCCCCEEEEECCC
VNGTHNLLAAIVESGLDIHIVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCHHHHHHHHHHHCCCEEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTEATARDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHICDSVKCVQLALENPPAKGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCCCEEHHH
NQMTESHQVGELAKKVAALTGAELNHLPNPRNEAVENDLIVDNRCFIELGLKPTTLDDGL
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHCCCEEECCEEEEEECCCCCCCCCHH
LAEVVDVAKRWADRCDRSRIPCVSAWTSTQAEAIKHPS
HHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA