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The map label for this gene is murB

Identifier: 124021740

GI number: 124021740

Start: 28396

End: 29319

Strand: Reverse

Name: murB

Synonym: P9303_00251

Alternate gene names: 124021740

Gene position: 29319-28396 (Counterclockwise)

Preceding gene: 124021741

Following gene: 124021739

Centisome position: 1.09

GC content: 54.98

Gene sequence:

>924_bases
ATGCCCACCCTCCCTGGCCGCCTGACCATAGTGAAGGGGATCAAGCCCCAACCACTGGTTTCTCTTGCCAACTTCACGAG
CTGGCGCGTCGGAGGGCCTGCGGAATGGTTTGCATCGCCCAGCAGCGTGGAGGAACTACAGACACTGATTGCTTGGGCCT
ATGAACAGAAGATGCCCTGTCGAGTCATCGGAGCAGGATCAAACCTACTGATCAACGACGCTGGCTTGCCGGGCCTGAGC
ATTTGCATGCGCAAACTCCAAGGGAGCGACCTAGATCCAAAGACTGGCATTGTCGAAGCCTTGGCCGGTGAACCAATCCC
CAACCTTTCCAAACGCGCCGCAAAGGCTGGCCTGCATGGCCTGGAATGGGCCGTTGGGATTCCTGGAACAGTTGGTGGTG
CAACCGTCATGAATGCTGGAGCCCAGGGCGGCTGCACAGCCGACTGGCTGGAATCTGTACAAGTCATCGACCTCAATGGC
GAGGGCCCTTTTGAACTGAGCCGCCAAGAGCTTGACTACGCCTACCGAAAAAGTCTCTTGCAGGAAAAAACACTCGTCGT
GCTCTCAGCCAGATTCCGCCTAGACCCCGGCCATGATTACAAGGAGCTCAATCAAATCACACATAAAAACCTCACTCATC
GCACGACCACCCAGCCTTATCAATTGCCTAGTTGCGGAAGTGTCTTTCGCAACCCAGAACCCCTCAAAGCTGGTCGTCTA
ATCGAAGCGCTGGGTCTGAAAGGTCACCGCATTGGAGGAGCTGAAGTCTCCCCCATTCACGCAAACTTCATCGTCAATAT
TGGTGGAGCCACGGCTGCCGACATCAATCAAATGATCAACCTCATTCAGCAACGGGTGCAGATGGCCCATGGAGTGATGC
TTCATCCTGAAGTGAAACGACTTGGTTTCGAAGCGACCGCTTAA

Upstream 100 bases:

>100_bases
TGCAAAAACACAGCCTCAAAGGTGACCTAGTCCTCGCCATGGGTGCAGGCAATATCAACAATCTATGGAGACAACTGACA
CGTCTTGACAACGCAAAGAC

Downstream 100 bases:

>100_bases
TCTGCCCATTCCTCCAGCAACGCAATGGCAGGGTTCGGACTTCCCAATTTCGGCCAACTCACCGAAGCCTTCCGCAAGGC
CCAACAAATCCAACAAAACG

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPTLPGRLTIVKGIKPQPLVSLANFTSWRVGGPAEWFASPSSVEELQTLIAWAYEQKMPCRVIGAGSNLLINDAGLPGLS
ICMRKLQGSDLDPKTGIVEALAGEPIPNLSKRAAKAGLHGLEWAVGIPGTVGGATVMNAGAQGGCTADWLESVQVIDLNG
EGPFELSRQELDYAYRKSLLQEKTLVVLSARFRLDPGHDYKELNQITHKNLTHRTTTQPYQLPSCGSVFRNPEPLKAGRL
IEALGLKGHRIGGAEVSPIHANFIVNIGGATAADINQMINLIQQRVQMAHGVMLHPEVKRLGFEATA

Sequences:

>Translated_307_residues
MPTLPGRLTIVKGIKPQPLVSLANFTSWRVGGPAEWFASPSSVEELQTLIAWAYEQKMPCRVIGAGSNLLINDAGLPGLS
ICMRKLQGSDLDPKTGIVEALAGEPIPNLSKRAAKAGLHGLEWAVGIPGTVGGATVMNAGAQGGCTADWLESVQVIDLNG
EGPFELSRQELDYAYRKSLLQEKTLVVLSARFRLDPGHDYKELNQITHKNLTHRTTTQPYQLPSCGSVFRNPEPLKAGRL
IEALGLKGHRIGGAEVSPIHANFIVNIGGATAADINQMINLIQQRVQMAHGVMLHPEVKRLGFEATA
>Mature_306_residues
PTLPGRLTIVKGIKPQPLVSLANFTSWRVGGPAEWFASPSSVEELQTLIAWAYEQKMPCRVIGAGSNLLINDAGLPGLSI
CMRKLQGSDLDPKTGIVEALAGEPIPNLSKRAAKAGLHGLEWAVGIPGTVGGATVMNAGAQGGCTADWLESVQVIDLNGE
GPFELSRQELDYAYRKSLLQEKTLVVLSARFRLDPGHDYKELNQITHKNLTHRTTTQPYQLPSCGSVFRNPEPLKAGRLI
EALGLKGHRIGGAEVSPIHANFIVNIGGATAADINQMINLIQQRVQMAHGVMLHPEVKRLGFEATA

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=308, Percent_Identity=25.974025974026, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_PROM3 (A2C5M2)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016047.1
- ProteinModelPortal:   A2C5M2
- SMR:   A2C5M2
- STRING:   A2C5M2
- GeneID:   4777840
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_00251
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   LESSKIP
- ProtClustDB:   PRK13905
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 33018; Mature: 32886

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 176-176 ACT_SITE 227-227 ACT_SITE 297-297

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLPGRLTIVKGIKPQPLVSLANFTSWRVGGPAEWFASPSSVEELQTLIAWAYEQKMPC
CCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCE
RVIGAGSNLLINDAGLPGLSICMRKLQGSDLDPKTGIVEALAGEPIPNLSKRAAKAGLHG
EEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCC
LEWAVGIPGTVGGATVMNAGAQGGCTADWLESVQVIDLNGEGPFELSRQELDYAYRKSLL
CEEEECCCCCCCCHHHHCCCCCCCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
QEKTLVVLSARFRLDPGHDYKELNQITHKNLTHRTTTQPYQLPSCGSVFRNPEPLKAGRL
HHHHEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHH
IEALGLKGHRIGGAEVSPIHANFIVNIGGATAADINQMINLIQQRVQMAHGVMLHPEVKR
HHHHCCCCCCCCCCEECCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEECCHHHH
LGFEATA
CCCCCCC
>Mature Secondary Structure 
PTLPGRLTIVKGIKPQPLVSLANFTSWRVGGPAEWFASPSSVEELQTLIAWAYEQKMPC
CCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCE
RVIGAGSNLLINDAGLPGLSICMRKLQGSDLDPKTGIVEALAGEPIPNLSKRAAKAGLHG
EEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCC
LEWAVGIPGTVGGATVMNAGAQGGCTADWLESVQVIDLNGEGPFELSRQELDYAYRKSLL
CEEEECCCCCCCCHHHHCCCCCCCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
QEKTLVVLSARFRLDPGHDYKELNQITHKNLTHRTTTQPYQLPSCGSVFRNPEPLKAGRL
HHHHEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHH
IEALGLKGHRIGGAEVSPIHANFIVNIGGATAADINQMINLIQQRVQMAHGVMLHPEVKR
HHHHCCCCCCCCCCEECCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEECCHHHH
LGFEATA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA