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The map label for this gene is rpoD4 [H]

Identifier: 124026883

GI number: 124026883

Start: 1836223

End: 1837245

Strand: Direct

Name: rpoD4 [H]

Synonym: NATL1_21781

Alternate gene names: 124026883

Gene position: 1836223-1837245 (Clockwise)

Preceding gene: 124026882

Following gene: 124026884

Centisome position: 98.47

GC content: 40.76

Gene sequence:

>1023_bases
ATGGGGATCCCGCTGGAATCTGCCAAGGAAATTTCTGATGTTTCATTGGGAAAATCTAATTCGGCAAGCACAAGTCAAAA
ACTATCTGCATCAACTGTAAGTAGTCAAAAATCTCGAAGTTCTAGAAGACAAAGTAATCGTTTAGCTACTGATGCGATAG
GTTTCTATCTAACAAGTATTGGCAGAGTCCCACTTCTGACTCCTGCAGAAGAAATTGAGCTTGCTCATCATGTTCAACAA
ATGAAAGATTTGTTGAACCTTCCTCTTGAAGAACGCTCTACCCGTCAGAAGCACAAAATAAAAATGGGTAAACGGGCAAG
AGATCGCATGATGGCTGCAAATCTTAGGCTTGTTGTAAGTGTTGCAAAAAAATATCAAAACCAAGGTCTCGAATTACTCG
ATTTAGTTCAAGAAGGTGCCATTGGATTAGAAAGAGCTGTCGATAAATTTGATCCTGCAATGGGTTATAAATTCTCAACC
TATGCTTACTGGTGGATAAGACAAGGGATGACTCGTGCCATAGATAACAGTGCTAGAACTATTCGACTGCCAATACATAT
CAGCGAAAAACTTTCAAAAATGCGCCGTATCTCGCGTGAATTATCTCATCGATTTGGTAGGCAACCAAATCGACTCGAGT
TGGCAAACGCGATGGGAATTGAACCTCAAGATCTAGAAGATCTAGTATCTCAAAGCGCGCCATGTGCCTCTCTTGATGCT
CATGCAAGAGGCGAAGAAGATCGTAGTACTCTAGGAGAACTAATTCCAGACCCAAATTCTGATGAGCCGATGGAAGGGAT
GGATAGAAGTATTCAAAAAGAACACCTCGGAGGCTGGTTATCTCAATTAAATGAGAGAGAGCAAAAAATCATGAGACTTC
GTTTTGGCCTTGATGGAGAAGAACCACTTACTCTTGCTGAAATTGGAAGACAAATAAATGTTTCTAGAGAGCGTGTAAGA
CAACTTGAGGCTAAAGCAATTTTAAAATTGAGGGTGATGACTACTCATCAAAACGCTGCCTAA

Upstream 100 bases:

>100_bases
CCTAATTAGGCGAAAAATCATAAATTTTTCGTTTCAATTAGTCAACTTCTCCTTATAAGTGCTAACTTCTTAATAAATCT
AATTTCAAGAGGCCAGGGTG

Downstream 100 bases:

>100_bases
ACATTGCCCATTCAAATAGCTGTTTTTGTTATTGCTTTATGGATAATTATAATTTTATTGATTGCATTTTTATGTAAGAG
ATATTTTCCTAAAAAAGAGG

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MGIPLESAKEISDVSLGKSNSASTSQKLSASTVSSQKSRSSRRQSNRLATDAIGFYLTSIGRVPLLTPAEEIELAHHVQQ
MKDLLNLPLEERSTRQKHKIKMGKRARDRMMAANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFST
YAYWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRISRELSHRFGRQPNRLELANAMGIEPQDLEDLVSQSAPCASLDA
HARGEEDRSTLGELIPDPNSDEPMEGMDRSIQKEHLGGWLSQLNEREQKIMRLRFGLDGEEPLTLAEIGRQINVSRERVR
QLEAKAILKLRVMTTHQNAA

Sequences:

>Translated_340_residues
MGIPLESAKEISDVSLGKSNSASTSQKLSASTVSSQKSRSSRRQSNRLATDAIGFYLTSIGRVPLLTPAEEIELAHHVQQ
MKDLLNLPLEERSTRQKHKIKMGKRARDRMMAANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFST
YAYWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRISRELSHRFGRQPNRLELANAMGIEPQDLEDLVSQSAPCASLDA
HARGEEDRSTLGELIPDPNSDEPMEGMDRSIQKEHLGGWLSQLNEREQKIMRLRFGLDGEEPLTLAEIGRQINVSRERVR
QLEAKAILKLRVMTTHQNAA
>Mature_339_residues
GIPLESAKEISDVSLGKSNSASTSQKLSASTVSSQKSRSSRRQSNRLATDAIGFYLTSIGRVPLLTPAEEIELAHHVQQM
KDLLNLPLEERSTRQKHKIKMGKRARDRMMAANLRLVVSVAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTY
AYWWIRQGMTRAIDNSARTIRLPIHISEKLSKMRRISRELSHRFGRQPNRLELANAMGIEPQDLEDLVSQSAPCASLDAH
ARGEEDRSTLGELIPDPNSDEPMEGMDRSIQKEHLGGWLSQLNEREQKIMRLRFGLDGEEPLTLAEIGRQINVSRERVRQ
LEAKAILKLRVMTTHQNAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=229, Percent_Identity=45.4148471615721, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=37.6306620209059, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1789871, Length=258, Percent_Identity=28.2945736434109, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=27.2277227722772, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38344; Mature: 38213

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIPLESAKEISDVSLGKSNSASTSQKLSASTVSSQKSRSSRRQSNRLATDAIGFYLTSI
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRVPLLTPAEEIELAHHVQQMKDLLNLPLEERSTRQKHKIKMGKRARDRMMAANLRLVVS
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTYAYWWIRQGMTRAIDNSART
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHCCCCCE
IRLPIHISEKLSKMRRISRELSHRFGRQPNRLELANAMGIEPQDLEDLVSQSAPCASLDA
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCH
HARGEEDRSTLGELIPDPNSDEPMEGMDRSIQKEHLGGWLSQLNEREQKIMRLRFGLDGE
HCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EPLTLAEIGRQINVSRERVRQLEAKAILKLRVMTTHQNAA
CCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
GIPLESAKEISDVSLGKSNSASTSQKLSASTVSSQKSRSSRRQSNRLATDAIGFYLTSI
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRVPLLTPAEEIELAHHVQQMKDLLNLPLEERSTRQKHKIKMGKRARDRMMAANLRLVVS
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKKYQNQGLELLDLVQEGAIGLERAVDKFDPAMGYKFSTYAYWWIRQGMTRAIDNSART
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHCCCCCE
IRLPIHISEKLSKMRRISRELSHRFGRQPNRLELANAMGIEPQDLEDLVSQSAPCASLDA
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCH
HARGEEDRSTLGELIPDPNSDEPMEGMDRSIQKEHLGGWLSQLNEREQKIMRLRFGLDGE
HCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EPLTLAEIGRQINVSRERVRQLEAKAILKLRVMTTHQNAA
CCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA