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The map label for this gene is pyrG

Identifier: 124026868

GI number: 124026868

Start: 1820216

End: 1821883

Strand: Direct

Name: pyrG

Synonym: NATL1_21631

Alternate gene names: 124026868

Gene position: 1820216-1821883 (Clockwise)

Preceding gene: 124026867

Following gene: 124026869

Centisome position: 97.61

GC content: 39.15

Gene sequence:

>1668_bases
ATGGCAAAATTTGTTTTCGTCACTGGTGGTGTAGTTTCAAGCATTGGCAAAGGAATTGTTGCCGCAAGTCTTGGCAGACT
ACTTAAATCAAAAGGGTACAGTGTTTCGATTTTGAAGCTTGACCCATATTTAAATGTTGATCCTGGGACGATGAGCCCAT
TTCAACATGGAGAAGTTTTTGTAACTGAAGACGGGGCTGAAACTGATTTGGACCTTGGGCATTATGAGCGCTTTACAGAC
ACTGCAATGTCAAGACTAAACAGTGTCACGACAGGTTCCATTTATCAAGCTGTTATTAATAAAGAAAGAAGAGGTGACTA
TGACGGAAGGACGGTTCAAGTAATCCCCCACATCACTCGTGAAATTCGCGAAAGAATTAAACGTGTAGCAAACAATAGTG
GTGCAGATGTAGTGATATCAGAAATTGGAGGAACAGTTGGAGATATTGAATCTCTACCTTTTCTTGAAGCAATAAGAGAA
TTTAAAGGAGATGTAAAAAGAAACGATGTTGTTTATGTTCACGTCACATTATTGCCTTACATAGGTACATCTGGAGAAAT
TAAAACCAAGCCAACACAGCACTCAGTAAAAGAATTGCGTTCTATAGGTATTCAGCCAGATATTTTAGTTTGTCGCAGTG
ATAGGCCTATTAATGATGAATTAAAAAATAAAATAGGGGGTTTCTGTGGAGTTAACTCTGAAGCGGTCATAGCATCCTTA
GATGCTGACAGTATTTACTCAGTACCATTAGCGCTCAAAGATGAGGGACTTTGCAAGGAAGTATTAGATTGCTTGGATCT
AAATGATCATGAAAGTGATTTAAAAGACTGGGAACGATTAGTCCATAAATTGCGCAATCCAGGTCCTTCTGTCAAAGTTG
CATTAGTTGGCAAATATGTACAATTAAATGATGCCTACTTATCAGTAGTCGAAGCATTACGTCACGCATGTATCTCGCAT
GATGCGTCTTTAGATCTTCACTGGATCAATGCGGAAAACATAGAATCCGAGGGAGCTGAAAAACTACTGCAGGGAATGGA
CGCAATTGTCGTTCCAGGAGGTTTTGGTAACCGTGGCGTCAATGGGAAAATAGCTGCTATTAGATGGGCAAGGGAGCAAA
GAGTACCTTTTTTAGGGCTTTGCTTAGGTATGCAATGTGCTGTCATTGAATGGGCTCGCAATATCGCTGGTTTAGAAGAT
GCATCTAGTGCCGAACTAAATCCGAATTCAAAACACCCAGTAATTCACTTACTTCCAGAACAACAAGACGTCGTTGATCT
AGGAGGAACAATGAGATTAGGCGTATATCCTTGTAGACTTCAAGCAAACACAACAGGACAAAGTTTATACAACGAAGAAG
TTGTTTACGAAAGGCATAGACATCGTTATGAATTCAATAATTCATACAGAACTCTACTAATGGAATCTGGGTATGTAATT
AGTGGTACTTCACCTGATGGTCGATTAGTAGAGTTAATAGAATTAAAAAATCACCCATTTTTTATTGCATGTCAATATCA
TCCAGAATTCCTCTCTAGACCAGGAAAACCACACCCTTTATTTGGTGGTTTGATTCAAGCCGCACAAATACGTGTACCAT
CCTCACCGAGTGAGGCATTTAATCCTCAATCAAAAATTATTGAAAAAAAATCACTAGAACAGCAATAG

Upstream 100 bases:

>100_bases
ACTAGAAATTAGCGGTAAAAACATTATCCTAAAGAGAAAATATGTAAATTTCTTGGCGTTTACCTAGATCAAGGTAGCTT
AAAGAATGATTGAAAAGTAA

Downstream 100 bases:

>100_bases
ATGGGATCTTCTCTACCAGTAGTTGAATGTTTTCATTCTCTACAAGGAGAAGGAGAACATGCAGGTAGAAGCGCTTACTT
CATCAGATTGGCTAGTTGTA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MAKFVFVTGGVVSSIGKGIVAASLGRLLKSKGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTD
TAMSRLNSVTTGSIYQAVINKERRGDYDGRTVQVIPHITREIRERIKRVANNSGADVVISEIGGTVGDIESLPFLEAIRE
FKGDVKRNDVVYVHVTLLPYIGTSGEIKTKPTQHSVKELRSIGIQPDILVCRSDRPINDELKNKIGGFCGVNSEAVIASL
DADSIYSVPLALKDEGLCKEVLDCLDLNDHESDLKDWERLVHKLRNPGPSVKVALVGKYVQLNDAYLSVVEALRHACISH
DASLDLHWINAENIESEGAEKLLQGMDAIVVPGGFGNRGVNGKIAAIRWAREQRVPFLGLCLGMQCAVIEWARNIAGLED
ASSAELNPNSKHPVIHLLPEQQDVVDLGGTMRLGVYPCRLQANTTGQSLYNEEVVYERHRHRYEFNNSYRTLLMESGYVI
SGTSPDGRLVELIELKNHPFFIACQYHPEFLSRPGKPHPLFGGLIQAAQIRVPSSPSEAFNPQSKIIEKKSLEQQ

Sequences:

>Translated_555_residues
MAKFVFVTGGVVSSIGKGIVAASLGRLLKSKGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTD
TAMSRLNSVTTGSIYQAVINKERRGDYDGRTVQVIPHITREIRERIKRVANNSGADVVISEIGGTVGDIESLPFLEAIRE
FKGDVKRNDVVYVHVTLLPYIGTSGEIKTKPTQHSVKELRSIGIQPDILVCRSDRPINDELKNKIGGFCGVNSEAVIASL
DADSIYSVPLALKDEGLCKEVLDCLDLNDHESDLKDWERLVHKLRNPGPSVKVALVGKYVQLNDAYLSVVEALRHACISH
DASLDLHWINAENIESEGAEKLLQGMDAIVVPGGFGNRGVNGKIAAIRWAREQRVPFLGLCLGMQCAVIEWARNIAGLED
ASSAELNPNSKHPVIHLLPEQQDVVDLGGTMRLGVYPCRLQANTTGQSLYNEEVVYERHRHRYEFNNSYRTLLMESGYVI
SGTSPDGRLVELIELKNHPFFIACQYHPEFLSRPGKPHPLFGGLIQAAQIRVPSSPSEAFNPQSKIIEKKSLEQQ
>Mature_554_residues
AKFVFVTGGVVSSIGKGIVAASLGRLLKSKGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDT
AMSRLNSVTTGSIYQAVINKERRGDYDGRTVQVIPHITREIRERIKRVANNSGADVVISEIGGTVGDIESLPFLEAIREF
KGDVKRNDVVYVHVTLLPYIGTSGEIKTKPTQHSVKELRSIGIQPDILVCRSDRPINDELKNKIGGFCGVNSEAVIASLD
ADSIYSVPLALKDEGLCKEVLDCLDLNDHESDLKDWERLVHKLRNPGPSVKVALVGKYVQLNDAYLSVVEALRHACISHD
ASLDLHWINAENIESEGAEKLLQGMDAIVVPGGFGNRGVNGKIAAIRWAREQRVPFLGLCLGMQCAVIEWARNIAGLEDA
SSAELNPNSKHPVIHLLPEQQDVVDLGGTMRLGVYPCRLQANTTGQSLYNEEVVYERHRHRYEFNNSYRTLLMESGYVIS
GTSPDGRLVELIELKNHPFFIACQYHPEFLSRPGKPHPLFGGLIQAAQIRVPSSPSEAFNPQSKIIEKKSLEQQ

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=552, Percent_Identity=46.3768115942029, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI28559085, Length=548, Percent_Identity=45.8029197080292, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI28559083, Length=548, Percent_Identity=45.8029197080292, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI221316689, Length=548, Percent_Identity=45.8029197080292, Blast_Score=483, Evalue=1e-136,
Organism=Escherichia coli, GI1789142, Length=535, Percent_Identity=54.392523364486, Blast_Score=586, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI25148299, Length=623, Percent_Identity=38.5232744783307, Blast_Score=426, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322563, Length=562, Percent_Identity=44.4839857651246, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=43.3691756272401, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24664469, Length=554, Percent_Identity=48.014440433213, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI21357815, Length=499, Percent_Identity=47.0941883767535, Blast_Score=426, Evalue=1e-119,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_PROM1 (A2C5F9)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015983.1
- ProteinModelPortal:   A2C5F9
- SMR:   A2C5F9
- STRING:   A2C5F9
- GeneID:   4779795
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_21631
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   PMAR167555:NATL1_21631-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 61258; Mature: 61126

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 507-507 ACT_SITE 509-509

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFVFVTGGVVSSIGKGIVAASLGRLLKSKGYSVSILKLDPYLNVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTDTAMSRLNSVTTGSIYQAVINKERRGDYDGRTVQVIPHITR
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHH
EIRERIKRVANNSGADVVISEIGGTVGDIESLPFLEAIREFKGDVKRNDVVYVHVTLLPY
HHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEC
IGTSGEIKTKPTQHSVKELRSIGIQPDILVCRSDRPINDELKNKIGGFCGVNSEAVIASL
CCCCCCEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEC
DADSIYSVPLALKDEGLCKEVLDCLDLNDHESDLKDWERLVHKLRNPGPSVKVALVGKYV
CCCCEEECCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
QLNDAYLSVVEALRHACISHDASLDLHWINAENIESEGAEKLLQGMDAIVVPGGFGNRGV
EECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCC
NGKIAAIRWAREQRVPFLGLCLGMQCAVIEWARNIAGLEDASSAELNPNSKHPVIHLLPE
CCEEEEEEEHHHHCCCEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCC
QQDVVDLGGTMRLGVYPCRLQANTTGQSLYNEEVVYERHRHRYEFNNSYRTLLMESGYVI
CCCHHHCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEE
SGTSPDGRLVELIELKNHPFFIACQYHPEFLSRPGKPHPLFGGLIQAAQIRVPSSPSEAF
ECCCCCCCEEEEEEECCCCEEEEEEECHHHHCCCCCCCCHHHHHHHHHEEECCCCCHHHC
NPQSKIIEKKSLEQQ
CCHHHHHHHHHCCCC
>Mature Secondary Structure 
AKFVFVTGGVVSSIGKGIVAASLGRLLKSKGYSVSILKLDPYLNVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTDTAMSRLNSVTTGSIYQAVINKERRGDYDGRTVQVIPHITR
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHH
EIRERIKRVANNSGADVVISEIGGTVGDIESLPFLEAIREFKGDVKRNDVVYVHVTLLPY
HHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEC
IGTSGEIKTKPTQHSVKELRSIGIQPDILVCRSDRPINDELKNKIGGFCGVNSEAVIASL
CCCCCCEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEC
DADSIYSVPLALKDEGLCKEVLDCLDLNDHESDLKDWERLVHKLRNPGPSVKVALVGKYV
CCCCEEECCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
QLNDAYLSVVEALRHACISHDASLDLHWINAENIESEGAEKLLQGMDAIVVPGGFGNRGV
EECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCC
NGKIAAIRWAREQRVPFLGLCLGMQCAVIEWARNIAGLEDASSAELNPNSKHPVIHLLPE
CCEEEEEEEHHHHCCCEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCC
QQDVVDLGGTMRLGVYPCRLQANTTGQSLYNEEVVYERHRHRYEFNNSYRTLLMESGYVI
CCCHHHCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEE
SGTSPDGRLVELIELKNHPFFIACQYHPEFLSRPGKPHPLFGGLIQAAQIRVPSSPSEAF
ECCCCCCCEEEEEEECCCCEEEEEEECHHHHCCCCCCCCHHHHHHHHHEEECCCCCHHHC
NPQSKIIEKKSLEQQ
CCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA