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The map label for this gene is sqdB [C]

Identifier: 124026840

GI number: 124026840

Start: 1791502

End: 1792695

Strand: Direct

Name: sqdB [C]

Synonym: NATL1_21351

Alternate gene names: 124026840

Gene position: 1791502-1792695 (Clockwise)

Preceding gene: 124026839

Following gene: 124026841

Centisome position: 96.07

GC content: 39.11

Gene sequence:

>1194_bases
GTGAAAGTTTTCGTTCTTGGTGGTGACGGCTTCTGCGGATGGCCTTGTGCAGTAAATCTTGCTGACAAAGGTCATGATGT
ATTCATCGTGGATAATCTGAGTCGTCGAAAAATCGATATAGACCTTGAAGTTGAATCCTTAACTCCAATTACAAGTATTG
GAGAAAGGATTAAAGCTTGGTCTGAGATAGGCGGAAAACCTATTCAATTTATTCATTTAGACCTTGCCTCTGAATATCAA
AAGCTTTTAGATCTGTTAATCGAGGAAAAGCCGGATTCAATAATTCATTTTGCTGAACAACGTGCTGCTCCATATTCCAT
GAAAAGCAGCGCAACCAAGAGATATACAGTTGATAACAATGTCAATGGTACTCATAATCTCCTTGCCGCAATTGTTGAAT
CTCAATTAGATATTCACATTGTTCATCTTGGGACAATGGGAGTTTATGGCTATGGATCTCATAGAGGCGCGACCATTCCT
GAAGGTTACTTAAAAGTGGAAGTACCCCAACCAGATGGCAGTCGTTTTGAGGAAGAAATCCTTCATCCAGCAAGTCCTGG
CAGCGTTTATCACATGACAAAAACGCTTGATCAATTGCTCTTTCTTTACTACAACAAAAATGACCAGATCAGAATCACTG
ATCTTCATCAAGGAATTGTATGGGGAACGAATACTGATGTCACAAGTCGTGACCCAAGATTGACTAATCGATTTGACTAT
GACGGTGATTATGGAACAGTGTTAAATCGATTTTTAATGCAGGCGGCCATTGGATACCCATTAACTGTTCATGGTACTGG
TGGACAAACAAGAGCTTTTATACATATTCAAGATTCAGTAAAATGTGTTCAATTGGCACTCGAGAACCCTCCTGAGAAAG
GTGAAAGGGTCAAAATTTTCAACCAAATGACGGAAAGTCACCAAGTTGGGGAATTAGCTAAAAAAGTAGCCTCTCTCACT
GGAGCAAAAATTAATTATCTGCCAAACCCAAGAAACGAAGCGGTTGAAAATGATTTGATAGTTGATAATAGATGTTTTAT
TGAGCTTGGATTAGATCCGACAACTCTCGATAATGGACTTTTAGAAGAAGTGGTTAATGTCGCGAAAAAATTCTCCAATC
GCTGTGATTTAAAACGAATACCTTGTGTATCTGCATGGACATCAACCCAAGCAAAAGCAATCCATAAATCCTAA

Upstream 100 bases:

>100_bases
TCCTTGTAATTGGGCTTATCGCATATTCTTTAACAATCAAGTGAAACCCTTGTAGTAGCTTGAATAATATGAGTGTTTGC
TCTGTCTTTTGGAGTTTAGA

Downstream 100 bases:

>100_bases
ACCTAACTAGTACAAAATCTTGAAAAGACACGCTCAGTGAAAATAGCTTTCTTTACAGAAACTTTCCTACCCAAAGTTGA
TGGAATAGTCACTCGTTTAA

Product: sulfolipid (UDP-sulfoquinovose) biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Nucleoside-Diphosphate-Sugar Epimerase; Sulfolipid Biosynthesis Protein SqdB; DTDP-Glucose 4 6-Dehydratase; DTDP-Glucose 4-6-Dehydratase; Epimerase; NAD Dependent Epimerase/Dehydratase Family Protein; Nucleotide Sugar Epimerase/Dehydratase; UDP-Glucose 4-Epimerase; UDP Sulfoquinovose Synthase

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKVFVLGGDGFCGWPCAVNLADKGHDVFIVDNLSRRKIDIDLEVESLTPITSIGERIKAWSEIGGKPIQFIHLDLASEYQ
KLLDLLIEEKPDSIIHFAEQRAAPYSMKSSATKRYTVDNNVNGTHNLLAAIVESQLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTDVTSRDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIQDSVKCVQLALENPPEKGERVKIFNQMTESHQVGELAKKVASLT
GAKINYLPNPRNEAVENDLIVDNRCFIELGLDPTTLDNGLLEEVVNVAKKFSNRCDLKRIPCVSAWTSTQAKAIHKS

Sequences:

>Translated_397_residues
MKVFVLGGDGFCGWPCAVNLADKGHDVFIVDNLSRRKIDIDLEVESLTPITSIGERIKAWSEIGGKPIQFIHLDLASEYQ
KLLDLLIEEKPDSIIHFAEQRAAPYSMKSSATKRYTVDNNVNGTHNLLAAIVESQLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTDVTSRDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIQDSVKCVQLALENPPEKGERVKIFNQMTESHQVGELAKKVASLT
GAKINYLPNPRNEAVENDLIVDNRCFIELGLDPTTLDNGLLEEVVNVAKKFSNRCDLKRIPCVSAWTSTQAKAIHKS
>Mature_397_residues
MKVFVLGGDGFCGWPCAVNLADKGHDVFIVDNLSRRKIDIDLEVESLTPITSIGERIKAWSEIGGKPIQFIHLDLASEYQ
KLLDLLIEEKPDSIIHFAEQRAAPYSMKSSATKRYTVDNNVNGTHNLLAAIVESQLDIHIVHLGTMGVYGYGSHRGATIP
EGYLKVEVPQPDGSRFEEEILHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTDVTSRDPRLTNRFDY
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIQDSVKCVQLALENPPEKGERVKIFNQMTESHQVGELAKKVASLT
GAKINYLPNPRNEAVENDLIVDNRCFIELGLDPTTLDNGLLEEVVNVAKKFSNRCDLKRIPCVSAWTSTQAKAIHKS

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 44257; Mature: 44257

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFVLGGDGFCGWPCAVNLADKGHDVFIVDNLSRRKIDIDLEVESLTPITSIGERIKAW
CEEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
SEIGGKPIQFIHLDLASEYQKLLDLLIEEKPDSIIHFAEQRAAPYSMKSSATKRYTVDNN
HHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHCCCCEEEEECCC
VNGTHNLLAAIVESQLDIHIVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCHHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTDVTSRDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIQDSVKCVQLALENPPEKGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEHHHHCCCCCCCCEEHHH
NQMTESHQVGELAKKVASLTGAKINYLPNPRNEAVENDLIVDNRCFIELGLDPTTLDNGL
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHCCCEEECCEEEEEECCCCCCCCHHH
LEEVVNVAKKFSNRCDLKRIPCVSAWTSTQAKAIHKS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MKVFVLGGDGFCGWPCAVNLADKGHDVFIVDNLSRRKIDIDLEVESLTPITSIGERIKAW
CEEEEEECCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHH
SEIGGKPIQFIHLDLASEYQKLLDLLIEEKPDSIIHFAEQRAAPYSMKSSATKRYTVDNN
HHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHCCCCEEEEECCC
VNGTHNLLAAIVESQLDIHIVHLGTMGVYGYGSHRGATIPEGYLKVEVPQPDGSRFEEEI
CCHHHHHHHHHHHCCCCEEEEEEECEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LHPASPGSVYHMTKTLDQLLFLYYNKNDQIRITDLHQGIVWGTNTDVTSRDPRLTNRFDY
CCCCCCCCEEHHHHHHHHHEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCCCCCCC
DGDYGTVLNRFLMQAAIGYPLTVHGTGGQTRAFIHIQDSVKCVQLALENPPEKGERVKIF
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEHHHHCCCCCCCCEEHHH
NQMTESHQVGELAKKVASLTGAKINYLPNPRNEAVENDLIVDNRCFIELGLDPTTLDNGL
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHCCCEEECCEEEEEECCCCCCCCHHH
LEEVVNVAKKFSNRCDLKRIPCVSAWTSTQAKAIHKS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA