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The map label for this gene is gmd [H]

Identifier: 124026801

GI number: 124026801

Start: 1740047

End: 1741099

Strand: Direct

Name: gmd [H]

Synonym: NATL1_20961

Alternate gene names: 124026801

Gene position: 1740047-1741099 (Clockwise)

Preceding gene: 124026797

Following gene: 124026802

Centisome position: 93.31

GC content: 35.42

Gene sequence:

>1053_bases
GTGAGTCAAAAGAAAATTGCTTTAATCACTGGCATAACTGGTCAAGATGGAAGCTACCTAGCGGAACTTCTACTTGATAA
AGGATATAAAGTTCATGGACTCGTTAGGAGATCAAGTCAGAAAAATACCAATCTTTTAGATAACATTTTAAATAGTCAGC
ACAATAAAAATCTTGAACTTCACTATGGTGATTTAACACAGAGCACCAATATCCTTAGAATAATTGAAAATATACAACCA
GATGAGATCTACAATTTAGGAGCGCAAAGCCATGTCCAAGTAAGTTTCGAGACGCCAGAATATACTGCCCAAACTGATGC
ATTAGGTCCTCTAAGGATACTTGAAGCTATTAGGATTCTGCAACTCACCAAAAAAACAAAAATTTATCAAGCCAGTACAT
CAGAATTGTATGGACTGGTACAAGAAACACCTCAAAACGAGAGAACACCCTTTTACCCAAGAAGTCCATATGGAGTCGCG
AAACTTTATGCATATTGGATAACAATCAATTATCGAGAATCGTATGGGATTTTCGCTTGTAACGGAATTTTATTTAATCA
TGAATCTCCTAGACGTGGAGAAAATTTTGTAACAAGAAAAATCACCAAAGGATTGTGCGAAATCAACAGGGGTTCAACTG
ACTGCCTATATCTAGGGAATATAGATTCTTTGAGGGACTGGGGGCATGCAAAAGATTATGTCGAAATGCAGTGGATGATG
CTTCAACAAGAAAAACCTGAAGATTATGTGATTTCAACAGGTAAACAAACTTCTGTTAGGAGATTTGTTGAATTATGTGC
AGAACATTTAAATTGGGGCGGAATTATTTGGGAAGGCAAGGGGATAGATGAAATAGGGAAGAGAAAAGATAATAAACAAG
TAATTATCAGGATTGATCCAAATTTATTTCGACCCGCAGAAGTGAATAGCCTATTGGGAGACTCAGAAAAAGCCTACAAA
AAACTTGGTTGGAAACCTAAATATAATATCGAGAGTCTGATAATTGAAATGATCGAAGAAGATAAAATAGACAAGAAATA
TAATTATAATTAA

Upstream 100 bases:

>100_bases
TTAGGATAAAAATAATATAATAAATAAATTATTTTTGCACAACTCTTTATATCAAAGTGTACTCAATAGAGTAGTATTAC
CGAAACGTTATTTTATTAGC

Downstream 100 bases:

>100_bases
ATTCTAAAATTCATTAGAAATAATCATAAATGCTTACGTTTAATGATATAAAAAGTTCAGAGCTTATAATACACACAAGA
AAAGTTCTTCTTAATAACGA

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSQKKIALITGITGQDGSYLAELLLDKGYKVHGLVRRSSQKNTNLLDNILNSQHNKNLELHYGDLTQSTNILRIIENIQP
DEIYNLGAQSHVQVSFETPEYTAQTDALGPLRILEAIRILQLTKKTKIYQASTSELYGLVQETPQNERTPFYPRSPYGVA
KLYAYWITINYRESYGIFACNGILFNHESPRRGENFVTRKITKGLCEINRGSTDCLYLGNIDSLRDWGHAKDYVEMQWMM
LQQEKPEDYVISTGKQTSVRRFVELCAEHLNWGGIIWEGKGIDEIGKRKDNKQVIIRIDPNLFRPAEVNSLLGDSEKAYK
KLGWKPKYNIESLIIEMIEEDKIDKKYNYN

Sequences:

>Translated_350_residues
MSQKKIALITGITGQDGSYLAELLLDKGYKVHGLVRRSSQKNTNLLDNILNSQHNKNLELHYGDLTQSTNILRIIENIQP
DEIYNLGAQSHVQVSFETPEYTAQTDALGPLRILEAIRILQLTKKTKIYQASTSELYGLVQETPQNERTPFYPRSPYGVA
KLYAYWITINYRESYGIFACNGILFNHESPRRGENFVTRKITKGLCEINRGSTDCLYLGNIDSLRDWGHAKDYVEMQWMM
LQQEKPEDYVISTGKQTSVRRFVELCAEHLNWGGIIWEGKGIDEIGKRKDNKQVIIRIDPNLFRPAEVNSLLGDSEKAYK
KLGWKPKYNIESLIIEMIEEDKIDKKYNYN
>Mature_349_residues
SQKKIALITGITGQDGSYLAELLLDKGYKVHGLVRRSSQKNTNLLDNILNSQHNKNLELHYGDLTQSTNILRIIENIQPD
EIYNLGAQSHVQVSFETPEYTAQTDALGPLRILEAIRILQLTKKTKIYQASTSELYGLVQETPQNERTPFYPRSPYGVAK
LYAYWITINYRESYGIFACNGILFNHESPRRGENFVTRKITKGLCEINRGSTDCLYLGNIDSLRDWGHAKDYVEMQWMML
QQEKPEDYVISTGKQTSVRRFVELCAEHLNWGGIIWEGKGIDEIGKRKDNKQVIIRIDPNLFRPAEVNSLLGDSEKAYKK
LGWKPKYNIESLIIEMIEEDKIDKKYNYN

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=341, Percent_Identity=57.1847507331378, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1788366, Length=349, Percent_Identity=63.323782234957, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=24.1477272727273, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994969, Length=336, Percent_Identity=24.4047619047619, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=350, Percent_Identity=56, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17539424, Length=350, Percent_Identity=56, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17539422, Length=350, Percent_Identity=56, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI133901786, Length=350, Percent_Identity=56, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI133901790, Length=350, Percent_Identity=56, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17507723, Length=343, Percent_Identity=57.1428571428571, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=56.5597667638484, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 40336; Mature: 40205

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKKIALITGITGQDGSYLAELLLDKGYKVHGLVRRSSQKNTNLLDNILNSQHNKNLEL
CCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEE
HYGDLTQSTNILRIIENIQPDEIYNLGAQSHVQVSFETPEYTAQTDALGPLRILEAIRIL
EECCCCCCCHHHHHHHCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
QLTKKTKIYQASTSELYGLVQETPQNERTPFYPRSPYGVAKLYAYWITINYRESYGIFAC
HHHHHHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEEEEEECCCCEEEE
NGILFNHESPRRGENFVTRKITKGLCEINRGSTDCLYLGNIDSLRDWGHAKDYVEMQWMM
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHH
LQQEKPEDYVISTGKQTSVRRFVELCAEHLNWGGIIWEGKGIDEIGKRKDNKQVIIRIDP
HHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCEEEEEECC
NLFRPAEVNSLLGDSEKAYKKLGWKPKYNIESLIIEMIEEDKIDKKYNYN
CCCCCHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SQKKIALITGITGQDGSYLAELLLDKGYKVHGLVRRSSQKNTNLLDNILNSQHNKNLEL
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEE
HYGDLTQSTNILRIIENIQPDEIYNLGAQSHVQVSFETPEYTAQTDALGPLRILEAIRIL
EECCCCCCCHHHHHHHCCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
QLTKKTKIYQASTSELYGLVQETPQNERTPFYPRSPYGVAKLYAYWITINYRESYGIFAC
HHHHHHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEEEEEECCCCEEEE
NGILFNHESPRRGENFVTRKITKGLCEINRGSTDCLYLGNIDSLRDWGHAKDYVEMQWMM
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHH
LQQEKPEDYVISTGKQTSVRRFVELCAEHLNWGGIIWEGKGIDEIGKRKDNKQVIIRIDP
HHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCEEEEEECC
NLFRPAEVNSLLGDSEKAYKKLGWKPKYNIESLIIEMIEEDKIDKKYNYN
CCCCCHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]