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The map label for this gene is truA [H]

Identifier: 124026684

GI number: 124026684

Start: 1633577

End: 1634407

Strand: Reverse

Name: truA [H]

Synonym: NATL1_19791

Alternate gene names: 124026684

Gene position: 1634407-1633577 (Counterclockwise)

Preceding gene: 124026685

Following gene: 124026683

Centisome position: 87.65

GC content: 36.58

Gene sequence:

>831_bases
TTGACTAATAGAATTGCTCTTGTCATTCAATACGACGGATTAGGTTTTAGAGGCTGGCAAAATCAAAAAGATTCAATAAC
TGTTCAAGGAACTTTAGAAGAAAAAATTGCAGAACTTGATCCAATCAGACCAGTCAAGGCAATTGCAGCAGGCAGAACTG
ATTCAGGAGTTCATGCTTCAGGACAAGTTGTTCATTTTGATTGCTCTGGCCCAATTCCTATTCATAAATGGGCATCAGCC
TTGAATGGCAGACTTCCAGCATCAATTAAAGTTCTTGAAGCAGTAAGTGTTCCAAGTGCTTGGCATGCTTGCTATTCGGC
AAAATATCGAAGGTATAGATATTCTATTTTTAATGGTTCCTATCCAAATCTTTTTCTTCAAAATATAAGTTGGCATAAAT
ATAGATTTAAGTTGGACATCAATTTAATGAAGCAGGCTTTGAATGATTTACTAGGTTTACATGATTTTGCAGCGTTTCAA
AAAGCAGGTAGTAATAGAAGTAATTCATTGACCACAGTGCAAGATGTTTCTATTTCTCGTGATGGAGATATAGTCACAGT
TGAAATTCAGGCAAGTGGCTTTTTGTACGGGATGGTCAGGCTTTTGATGGGTCAATTAGTTGCTGTTGGAGAAAAACGAC
TTTCTCTTGAAAAATTTAGATATAGATGGAGAAAGGGATTGCGTTCTGAGGTGAAAGAGGCTGCTCCTGCTCATGGACTG
TGTTTGATAAGAGTTGGTTATGAGGAAAAAATCTTTTCAAAAGAGAAAAGTTTTGACACTTTTCCAAGTTTTTATCTACC
AAAATTTGAGCCTACGAAACATACAAGCTAA

Upstream 100 bases:

>100_bases
CCCAAGACGCGGCGATAATTCTGAAATGGCCATTGTTGAACTTGTATAGGATATTAATTTTTCAAAAACAAATCAATATT
TTTTTACGTATTAGTTCAAT

Downstream 100 bases:

>100_bases
ATAAAAGATTAATAAATTAAAAATATATGAATTTTGTTAAATCAGTAATTTAAATTTCATCCGAACAAAAAGGCCAGTTT
TAAGCCATGAAAGTGTAGAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTNRIALVIQYDGLGFRGWQNQKDSITVQGTLEEKIAELDPIRPVKAIAAGRTDSGVHASGQVVHFDCSGPIPIHKWASA
LNGRLPASIKVLEAVSVPSAWHACYSAKYRRYRYSIFNGSYPNLFLQNISWHKYRFKLDINLMKQALNDLLGLHDFAAFQ
KAGSNRSNSLTTVQDVSISRDGDIVTVEIQASGFLYGMVRLLMGQLVAVGEKRLSLEKFRYRWRKGLRSEVKEAAPAHGL
CLIRVGYEEKIFSKEKSFDTFPSFYLPKFEPTKHTS

Sequences:

>Translated_276_residues
MTNRIALVIQYDGLGFRGWQNQKDSITVQGTLEEKIAELDPIRPVKAIAAGRTDSGVHASGQVVHFDCSGPIPIHKWASA
LNGRLPASIKVLEAVSVPSAWHACYSAKYRRYRYSIFNGSYPNLFLQNISWHKYRFKLDINLMKQALNDLLGLHDFAAFQ
KAGSNRSNSLTTVQDVSISRDGDIVTVEIQASGFLYGMVRLLMGQLVAVGEKRLSLEKFRYRWRKGLRSEVKEAAPAHGL
CLIRVGYEEKIFSKEKSFDTFPSFYLPKFEPTKHTS
>Mature_275_residues
TNRIALVIQYDGLGFRGWQNQKDSITVQGTLEEKIAELDPIRPVKAIAAGRTDSGVHASGQVVHFDCSGPIPIHKWASAL
NGRLPASIKVLEAVSVPSAWHACYSAKYRRYRYSIFNGSYPNLFLQNISWHKYRFKLDINLMKQALNDLLGLHDFAAFQK
AGSNRSNSLTTVQDVSISRDGDIVTVEIQASGFLYGMVRLLMGQLVAVGEKRLSLEKFRYRWRKGLRSEVKEAAPAHGLC
LIRVGYEEKIFSKEKSFDTFPSFYLPKFEPTKHTS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=34.7985347985348, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=34.412955465587, Blast_Score=139, Evalue=1e-34,
Organism=Drosophila melanogaster, GI116007752, Length=307, Percent_Identity=27.6872964169381, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31117; Mature: 30986

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRIALVIQYDGLGFRGWQNQKDSITVQGTLEEKIAELDPIRPVKAIAAGRTDSGVHAS
CCCEEEEEEEECCCCCCCCCCCCCCEEEEECHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
GQVVHFDCSGPIPIHKWASALNGRLPASIKVLEAVSVPSAWHACYSAKYRRYRYSIFNGS
CCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHEEEEEECCC
YPNLFLQNISWHKYRFKLDINLMKQALNDLLGLHDFAAFQKAGSNRSNSLTTVQDVSISR
CCCCEEECCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECC
DGDIVTVEIQASGFLYGMVRLLMGQLVAVGEKRLSLEKFRYRWRKGLRSEVKEAAPAHGL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
CLIRVGYEEKIFSKEKSFDTFPSFYLPKFEPTKHTS
EEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNRIALVIQYDGLGFRGWQNQKDSITVQGTLEEKIAELDPIRPVKAIAAGRTDSGVHAS
CCEEEEEEEECCCCCCCCCCCCCCEEEEECHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
GQVVHFDCSGPIPIHKWASALNGRLPASIKVLEAVSVPSAWHACYSAKYRRYRYSIFNGS
CCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHEEEEEECCC
YPNLFLQNISWHKYRFKLDINLMKQALNDLLGLHDFAAFQKAGSNRSNSLTTVQDVSISR
CCCCEEECCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECC
DGDIVTVEIQASGFLYGMVRLLMGQLVAVGEKRLSLEKFRYRWRKGLRSEVKEAAPAHGL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
CLIRVGYEEKIFSKEKSFDTFPSFYLPKFEPTKHTS
EEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA