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The map label for this gene is tufA [H]

Identifier: 124026654

GI number: 124026654

Start: 1600858

End: 1602057

Strand: Reverse

Name: tufA [H]

Synonym: NATL1_19491

Alternate gene names: 124026654

Gene position: 1602057-1600858 (Counterclockwise)

Preceding gene: 124026655

Following gene: 124026653

Centisome position: 85.91

GC content: 41.83

Gene sequence:

>1200_bases
ATGGCTCGCGAGAAGTTTGAGAGGAACAAACCTCACGTCAACATAGGTACTATTGGCCACGTTGACCATGGCAAAACAAC
CCTTACAGCAGCAATTACAAAGGTTTTAGCAAAGAAAGGTCAAGCCGAAGCGCAAGATTACGCTGAAATTGATGGAGCTC
CAGAAGAGCGTGAACGCGGCATAACAATCAACACTGCTCACGTTGAATATGAAACTGATGGCAGGCATTACGCTCACGTT
GATTGCCCAGGTCACGCTGATTATGTAAAAAACATGATCACAGGTGCTGCTCAAATGGACGGTGCGATTCTTGTTGTAGC
AGCTACCGATGGAGCAATGGCACAAACAAAAGAACATATTCTTTTGGCGAAGCAGGTTGGTGTTCCAGCTTTGGTTGTTG
CACTAAACAAATGTGACATGGTCGATGATGAAGAAATGATAGAACTTGTAGAAATGGAGATTCGTGAACTTCTTACAAGT
TATGACTTCCCCGGCGATGACATCCCTGTCGTTCAAGTTTCGGGATTAAAAGCTATCGAAGGAGAAGCAGATTGGGAGAC
TAAAATAGATGACTTGATGACAGCTGTTGATGCATCAATTCCAGAACCAGAACGTGAAATTGATAAGCCTTTCTTAATGG
CTGTTGAAGACGTTTTTTCAATTACTGGACGTGGAACAGTTGCTACTGGAAGAATTGAAAGAGGAAAAGTAACAGTTGGA
GAGGAAGTTGAAATTGTAGGAATTAGAGATACAAGACTAACCACTGTTACCGGTGTCGAGATGTTTAGAAAACTTCTTGA
TGAAGGTATGGCTGGTGACAACGTTGGACTTCTTTTACGGGGTATTCAGAAGGAAGATATCGAGAGGGGAATGGTTCTTG
TTAAAAAAGGTTCCATCACACCTCATACTAAGTTTGAGGGTGAAGTTTATGTATTAAAAAAAGAAGAGGGTGGCCGTCAT
ACACCATTCTTTGCTGGTTATCGACCACAGTTTTATATTCGTACTACTGATGTAACAGGTCAAATTACTGCATTCACATC
TGATGATGGCAGCAATGTCGAAATGGTTATGCCTGGGGACAGAATAAAAATGACAGGAGAATTGATAGCTCCTGTTGCTA
TAGAACAAGGTATGCGATTCGCTATTCGCGAAGGTGGACGTACAATTGGAGCAGGAGTAGTTTCTAAAATTATTGAATAA

Upstream 100 bases:

>100_bases
GGAAGTTCCTCGTAATGTTGCTGAGGCAATTATCTCCAAGAATCAGGGCAATTCCTGATCTCTAAAAATTTACTAACCAC
CCCCCGATTCTTTAAAAAAA

Downstream 100 bases:

>100_bases
TGATTCAGAGGATGACTTGTAATTTATTCAGGTCATCCTCTAAATTAATTAAACAAATGAACTAATTAGTTCACCCTTTA
AAGAACCTAGAAAACTTAAT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITKVLAKKGQAEAQDYAEIDGAPEERERGITINTAHVEYETDGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAATDGAMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEMIELVEMEIRELLTS
YDFPGDDIPVVQVSGLKAIEGEADWETKIDDLMTAVDASIPEPEREIDKPFLMAVEDVFSITGRGTVATGRIERGKVTVG
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKKGSITPHTKFEGEVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGDRIKMTGELIAPVAIEQGMRFAIREGGRTIGAGVVSKIIE

Sequences:

>Translated_399_residues
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITKVLAKKGQAEAQDYAEIDGAPEERERGITINTAHVEYETDGRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAATDGAMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEMIELVEMEIRELLTS
YDFPGDDIPVVQVSGLKAIEGEADWETKIDDLMTAVDASIPEPEREIDKPFLMAVEDVFSITGRGTVATGRIERGKVTVG
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKKGSITPHTKFEGEVYVLKKEEGGRH
TPFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGDRIKMTGELIAPVAIEQGMRFAIREGGRTIGAGVVSKIIE
>Mature_398_residues
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITKVLAKKGQAEAQDYAEIDGAPEERERGITINTAHVEYETDGRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVAATDGAMAQTKEHILLAKQVGVPALVVALNKCDMVDDEEMIELVEMEIRELLTSY
DFPGDDIPVVQVSGLKAIEGEADWETKIDDLMTAVDASIPEPEREIDKPFLMAVEDVFSITGRGTVATGRIERGKVTVGE
EVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKKGSITPHTKFEGEVYVLKKEEGGRHT
PFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGDRIKMTGELIAPVAIEQGMRFAIREGGRTIGAGVVSKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=400, Percent_Identity=54.75, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=30.8390022675737, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=30.8390022675737, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503475, Length=458, Percent_Identity=30.5676855895196, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=30.2013422818792, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI46094014, Length=435, Percent_Identity=29.6551724137931, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=29.4117647058824, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI194097354, Length=436, Percent_Identity=27.7522935779816, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI194018520, Length=436, Percent_Identity=27.7522935779816, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI194018522, Length=435, Percent_Identity=27.5862068965517, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI157426893, Length=274, Percent_Identity=25.9124087591241, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=27.6119402985075, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI53729339, Length=237, Percent_Identity=32.4894514767932, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI53729337, Length=237, Percent_Identity=32.4894514767932, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790412, Length=398, Percent_Identity=72.1105527638191, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=72.1105527638191, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI2367247, Length=331, Percent_Identity=32.3262839879154, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=32.7800829875519, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789108, Length=331, Percent_Identity=30.5135951661631, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=406, Percent_Identity=49.2610837438424, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=45.1282051282051, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=447, Percent_Identity=30.6487695749441, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=447, Percent_Identity=30.6487695749441, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=30.184331797235, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=30.184331797235, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=28.3813747228381, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32566303, Length=432, Percent_Identity=29.3981481481481, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=271, Percent_Identity=30.2583025830258, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=42.8571428571429, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=269, Percent_Identity=27.1375464684015, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17565438, Length=431, Percent_Identity=23.8979118329466, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=177, Percent_Identity=35.5932203389831, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.2241813602015, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=31.685393258427, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=31.685393258427, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=456, Percent_Identity=25.8771929824561, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320377, Length=446, Percent_Identity=28.6995515695067, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320863, Length=275, Percent_Identity=26.1818181818182, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=30.5439330543933, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.0740740740741, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=401, Percent_Identity=57.6059850374065, Blast_Score=453, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=401, Percent_Identity=57.6059850374065, Blast_Score=453, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=46.2915601023018, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=30.1552106430155, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=30.1552106430155, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=30.1552106430155, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=30.1552106430155, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=30.1339285714286, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=30.1339285714286, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.03300330033, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137380, Length=451, Percent_Identity=28.3813747228381, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24647048, Length=267, Percent_Identity=27.7153558052434, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647046, Length=267, Percent_Identity=27.7153558052434, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=36.969696969697, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78706572, Length=257, Percent_Identity=27.6264591439689, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24582462, Length=156, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43582; Mature: 43451

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITKVLAKKGQAEAQDYAEIDGAPEERERG
CCCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHCC
ITINTAHVEYETDGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGAMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEEMIELVEMEIRELLTSYDFPGDDIPVVQVSGLKAIE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GEADWETKIDDLMTAVDASIPEPEREIDKPFLMAVEDVFSITGRGTVATGRIERGKVTVG
CCCCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEECCEECCEEEEC
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKKGSIT
CEEEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCHHHEEECCCHHHHCCCEEEEECCCCC
PHTKFEGEVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCEEEEEEECCCCCCEEEEECCC
RIKMTGELIAPVAIEQGMRFAIREGGRTIGAGVVSKIIE
CEEECCHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AREKFERNKPHVNIGTIGHVDHGKTTLTAAITKVLAKKGQAEAQDYAEIDGAPEERERG
CCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHCC
ITINTAHVEYETDGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGAMAQTKEHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHE
LLAKQVGVPALVVALNKCDMVDDEEMIELVEMEIRELLTSYDFPGDDIPVVQVSGLKAIE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEC
GEADWETKIDDLMTAVDASIPEPEREIDKPFLMAVEDVFSITGRGTVATGRIERGKVTVG
CCCCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCEEEECCEECCEEEEC
EEVEIVGIRDTRLTTVTGVEMFRKLLDEGMAGDNVGLLLRGIQKEDIERGMVLVKKGSIT
CEEEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCHHHEEECCCHHHHCCCEEEEECCCCC
PHTKFEGEVYVLKKEEGGRHTPFFAGYRPQFYIRTTDVTGQITAFTSDDGSNVEMVMPGD
CCCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCCEEEEEEECCCCCCEEEEECCC
RIKMTGELIAPVAIEQGMRFAIREGGRTIGAGVVSKIIE
CEEECCHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA