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The map label for this gene is rne [H]

Identifier: 124026647

GI number: 124026647

Start: 1594832

End: 1596754

Strand: Direct

Name: rne [H]

Synonym: NATL1_19421

Alternate gene names: 124026647

Gene position: 1594832-1596754 (Clockwise)

Preceding gene: 124026643

Following gene: 124026648

Centisome position: 85.53

GC content: 33.28

Gene sequence:

>1923_bases
ATGCCCCAGCAAATTGTCATTGCTGAGCATTTGCGAATTGCCGCTCTGCTCACTGATGAGAGAATAGATGAATTAATCGT
CGCTCAAGGTAGCTACCAAATCGGAGATATTTTCTTAGGAACCGTTGAAAATGTTCTTCCTGGGATAGACGCTGCTTTTG
TCAATATTGGTGAAAGTGAAAAAAATGGCTTTATTCATGTAAATGATTTAGGTCCGCTTAGATTAAAAAAAGCAACTGCA
GGAATAACAGAGTTGCTCGAACCAAGGCAAAAGGTTTTGGTTCAGGTAATGAAAGAACCCACCGGGACAAAAGGGCCTAG
ATTAACTGGAAACATAGCTCTTCCTGGTAGATATCTAGTACTCCAACCTTATGGACAAGGGGTAAATATTTCGAGAAGAA
TAAGTACTGAAAGTGAAAGAAATCGACTTAGAGCATTAGGAGTATTAGTTAAGCCTCCAAGTACTGGACTTTTAATAAGA
ACAGAAGCGGAAGATATTTGTGAAGAATTTTTAATTGATGATCTTGAAAATCTTCTTAAACAATGGGAGCTTATTCAACA
AGCATCAGAGAGTTGCTCTCCGCCAATTCTTTTAAATAGAGATGAAGACTTTATTCATAGAATTCTTCGAGATCATACAG
GTCAAAATCTTACTGAGATTGTTGTAGATAATTCTGAAGCAATTGGTCGAGTTAAAAACTTCCTTGGCAAGGATAGTAAT
GAATTAACAATAGAATTACATAACGATTCGGAAAATATTTTGGAGAAATACAAAGTAATATCTTCAATTAATGAAGCATT
AAAACCTAGAGTTGATCTACCTTCGGGTGGCTATATAATAATAGAGCCAACAGAAGCTTTGACAGTTATTGATGTCAACT
CAGGCTCATTTACACGATCTGCGAATTCCAGAGAAACAGTATTATGGACTAATTGTGAAGCCGCTATTGAGATAGCAAGA
CAATTAAAATTAAGGAATATTGGTGGGGTTATTATTATTGATTTTATTGATATGGATACAAAAAGAGATCAACTTCAATT
ATTAGAACATTTTACATCTGCTATTAATGGAGACTCGGCGCGGCCACAAATAGCTTCACTTACAGAACTTGGACTTGTTG
AGCTCACTAGAAAAAGACAAGGTCAAAATATATATGAATTATTTGGAAAGACTTCTCCTAATTCTCAAGGGCAAGGTTAT
CTTCCAAGCATTACTATTCAAGACATAAATCCAACAACCCCATCTGAAGCTGGCGTAATCAATGCAACTTTAATATCAGG
CGAAGATATTCAATCTTTACAAGAAACTAATAACAAAAAAAAGCGTATAAATAAAACAAGAGATATAGAAGCAAACTTAA
GCAATGAAGAAAATAAATCATCTACAGACAACTCAAAAGCTATCTCCACGGATACAATTACTGAAGATATTCAAAAAGAG
AGTAATAATAAAAGGAAAGAAACAACGATAATAAATATCAATATGAATCAGAATGAAGAGATTGTATATAGTTTGATGGG
ATTAGATCCTATTTTACTTTTAGAGAAACCTCCACTATCTGAAAACTATAAGGTTAATATAATCAGACCTGGGAAAAAGG
AAGCTAGAGAAGAAAAAAATAACATACCTGAGGATAATCAACAAAAAATAGTTGATGATTCTATTAGCAAACATCAAAAT
AATAACAAGGATATTATTCGTCTTAAAAACAAAAGTAATATTGAACAAAAATCAACCAATTCTGACGTAAAAGAGAGTAT
TGAAGAAGAAAATATAAATGTAGCTTTGGATCAAGAAACAAATGAATTAATAAATATTAATCATAATTCAATAAGCGAAA
AGAATGAATTACCTTCCACCGATTCACAAGAAGTTAATGAGGATCCAAGACGAAAAAGAAGAAGGTCTTCAGCCTCTTCT
TAA

Upstream 100 bases:

>100_bases
AATCTCTTTAAGTTTGTTAGACGTTAAAAATTTCCAACAGTTCTTTATGCAGTTTTTCTGCTAATCAATTTTTCATAGCT
TAAAAAGCTCAACATTGTAT

Downstream 100 bases:

>100_bases
ATATGGAAGATATAAAATCTCTTATCGCTGGAGTTGATGAAGTGGGAAAAGGTTGTTTATTTGGCCCTGTTTTCGCGGCA
GCAGTTATTTTAAGCAAAGA

Product: ribonuclease E/G

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MPQQIVIAEHLRIAALLTDERIDELIVAQGSYQIGDIFLGTVENVLPGIDAAFVNIGESEKNGFIHVNDLGPLRLKKATA
GITELLEPRQKVLVQVMKEPTGTKGPRLTGNIALPGRYLVLQPYGQGVNISRRISTESERNRLRALGVLVKPPSTGLLIR
TEAEDICEEFLIDDLENLLKQWELIQQASESCSPPILLNRDEDFIHRILRDHTGQNLTEIVVDNSEAIGRVKNFLGKDSN
ELTIELHNDSENILEKYKVISSINEALKPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRETVLWTNCEAAIEIAR
QLKLRNIGGVIIIDFIDMDTKRDQLQLLEHFTSAINGDSARPQIASLTELGLVELTRKRQGQNIYELFGKTSPNSQGQGY
LPSITIQDINPTTPSEAGVINATLISGEDIQSLQETNNKKKRINKTRDIEANLSNEENKSSTDNSKAISTDTITEDIQKE
SNNKRKETTIININMNQNEEIVYSLMGLDPILLLEKPPLSENYKVNIIRPGKKEAREEKNNIPEDNQQKIVDDSISKHQN
NNKDIIRLKNKSNIEQKSTNSDVKESIEEENINVALDQETNELININHNSISEKNELPSTDSQEVNEDPRRKRRRSSASS

Sequences:

>Translated_640_residues
MPQQIVIAEHLRIAALLTDERIDELIVAQGSYQIGDIFLGTVENVLPGIDAAFVNIGESEKNGFIHVNDLGPLRLKKATA
GITELLEPRQKVLVQVMKEPTGTKGPRLTGNIALPGRYLVLQPYGQGVNISRRISTESERNRLRALGVLVKPPSTGLLIR
TEAEDICEEFLIDDLENLLKQWELIQQASESCSPPILLNRDEDFIHRILRDHTGQNLTEIVVDNSEAIGRVKNFLGKDSN
ELTIELHNDSENILEKYKVISSINEALKPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRETVLWTNCEAAIEIAR
QLKLRNIGGVIIIDFIDMDTKRDQLQLLEHFTSAINGDSARPQIASLTELGLVELTRKRQGQNIYELFGKTSPNSQGQGY
LPSITIQDINPTTPSEAGVINATLISGEDIQSLQETNNKKKRINKTRDIEANLSNEENKSSTDNSKAISTDTITEDIQKE
SNNKRKETTIININMNQNEEIVYSLMGLDPILLLEKPPLSENYKVNIIRPGKKEAREEKNNIPEDNQQKIVDDSISKHQN
NNKDIIRLKNKSNIEQKSTNSDVKESIEEENINVALDQETNELININHNSISEKNELPSTDSQEVNEDPRRKRRRSSASS
>Mature_639_residues
PQQIVIAEHLRIAALLTDERIDELIVAQGSYQIGDIFLGTVENVLPGIDAAFVNIGESEKNGFIHVNDLGPLRLKKATAG
ITELLEPRQKVLVQVMKEPTGTKGPRLTGNIALPGRYLVLQPYGQGVNISRRISTESERNRLRALGVLVKPPSTGLLIRT
EAEDICEEFLIDDLENLLKQWELIQQASESCSPPILLNRDEDFIHRILRDHTGQNLTEIVVDNSEAIGRVKNFLGKDSNE
LTIELHNDSENILEKYKVISSINEALKPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRETVLWTNCEAAIEIARQ
LKLRNIGGVIIIDFIDMDTKRDQLQLLEHFTSAINGDSARPQIASLTELGLVELTRKRQGQNIYELFGKTSPNSQGQGYL
PSITIQDINPTTPSEAGVINATLISGEDIQSLQETNNKKKRINKTRDIEANLSNEENKSSTDNSKAISTDTITEDIQKES
NNKRKETTIININMNQNEEIVYSLMGLDPILLLEKPPLSENYKVNIIRPGKKEAREEKNNIPEDNQQKIVDDSISKHQNN
NKDIIRLKNKSNIEQKSTNSDVKESIEEENINVALDQETNELININHNSISEKNELPSTDSQEVNEDPRRKRRRSSASS

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=402, Percent_Identity=36.5671641791045, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082235, Length=383, Percent_Identity=36.5535248041775, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 71628; Mature: 71496

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQIVIAEHLRIAALLTDERIDELIVAQGSYQIGDIFLGTVENVLPGIDAAFVNIGESE
CCCCEEHHHHHEEHHHHHHHHHHHHEEECCCCEEHHHHHHHHHHHHCCCCEEEEECCCCC
KNGFIHVNDLGPLRLKKATAGITELLEPRQKVLVQVMKEPTGTKGPRLTGNIALPGRYLV
CCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPYGQGVNISRRISTESERNRLRALGVLVKPPSTGLLIRTEAEDICEEFLIDDLENLLK
EECCCCCCCEEEECCCHHHHHHHHHEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHH
QWELIQQASESCSPPILLNRDEDFIHRILRDHTGQNLTEIVVDNSEAIGRVKNFLGKDSN
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCC
ELTIELHNDSENILEKYKVISSINEALKPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRS
EEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEECC
ANSRETVLWTNCEAAIEIARQLKLRNIGGVIIIDFIDMDTKRDQLQLLEHFTSAINGDSA
CCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
RPQIASLTELGLVELTRKRQGQNIYELFGKTSPNSQGQGYLPSITIQDINPTTPSEAGVI
CCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEE
NATLISGEDIQSLQETNNKKKRINKTRDIEANLSNEENKSSTDNSKAISTDTITEDIQKE
EEEEECCHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHH
SNNKRKETTIININMNQNEEIVYSLMGLDPILLLEKPPLSENYKVNIIRPGKKEAREEKN
CCCCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHC
NIPEDNQQKIVDDSISKHQNNNKDIIRLKNKSNIEQKSTNSDVKESIEEENINVALDQET
CCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHCCCHHHHHHHHHCCCEEEEECCC
NELININHNSISEKNELPSTDSQEVNEDPRRKRRRSSASS
CCEEECCCCCCCCCCCCCCCCCHHCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
PQQIVIAEHLRIAALLTDERIDELIVAQGSYQIGDIFLGTVENVLPGIDAAFVNIGESE
CCCEEHHHHHEEHHHHHHHHHHHHEEECCCCEEHHHHHHHHHHHHCCCCEEEEECCCCC
KNGFIHVNDLGPLRLKKATAGITELLEPRQKVLVQVMKEPTGTKGPRLTGNIALPGRYLV
CCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPYGQGVNISRRISTESERNRLRALGVLVKPPSTGLLIRTEAEDICEEFLIDDLENLLK
EECCCCCCCEEEECCCHHHHHHHHHEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHH
QWELIQQASESCSPPILLNRDEDFIHRILRDHTGQNLTEIVVDNSEAIGRVKNFLGKDSN
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCC
ELTIELHNDSENILEKYKVISSINEALKPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRS
EEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEECC
ANSRETVLWTNCEAAIEIARQLKLRNIGGVIIIDFIDMDTKRDQLQLLEHFTSAINGDSA
CCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
RPQIASLTELGLVELTRKRQGQNIYELFGKTSPNSQGQGYLPSITIQDINPTTPSEAGVI
CCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEE
NATLISGEDIQSLQETNNKKKRINKTRDIEANLSNEENKSSTDNSKAISTDTITEDIQKE
EEEEECCHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHH
SNNKRKETTIININMNQNEEIVYSLMGLDPILLLEKPPLSENYKVNIIRPGKKEAREEKN
CCCCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHC
NIPEDNQQKIVDDSISKHQNNNKDIIRLKNKSNIEQKSTNSDVKESIEEENINVALDQET
CCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHCCCHHHHHHHHHCCCEEEEECCC
NELININHNSISEKNELPSTDSQEVNEDPRRKRRRSSASS
CCEEECCCCCCCCCCCCCCCCCHHCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]