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The map label for this gene is murD [H]

Identifier: 124026491

GI number: 124026491

Start: 1465369

End: 1466778

Strand: Direct

Name: murD [H]

Synonym: NATL1_17861

Alternate gene names: 124026491

Gene position: 1465369-1466778 (Clockwise)

Preceding gene: 124026489

Following gene: 124026492

Centisome position: 78.58

GC content: 30.0

Gene sequence:

>1410_bases
TTGTCAAAACAAAAACAATCAAATCAAATATATATTGTACTGGGACTAGGCTGCTCAGGAGTAAGTGCAGCAAAACTACT
TAAATCAGAAGGAAAGAACGTTTTAGTTTTAGAGAATAATTCAAATGAAAATCTATTAAATATATCTAATGAATTAAAAT
CAGAAGGAATCAAAGTTATTTTACTTGACGAACCACTTCATATTAATAATTTCACGCCTTGGATAGAAAGTGTTTGTTCT
ATTACTGTAAGTCCAGGGATAGATTGGGAGCATATAGCTCTAAAAGAATTAAGAGGTAAGAATATCAATGTTCAGGGAGA
AGTAGAATTAGCTTGGGAAAGATTAACTCATATCCCTTCTATTGGTATAACTGGAACTAATGGGAAAACCACCGTCACTA
ACATGCTTAATCATATTCTTAAATTAAATAATTTGAATACTGATATGGGAGGAAATATTGGAAAAGCATTATCCAAAATT
GCATTAGAAAATATGAAAAATAATAACCAAGAGTTAAACTGGCTAGTACTAGAATTAAGTAGTTTCCAAATTGAAGGTTC
ACCAAAAGTAGCACCCACCATAGGTATATGGACAACATTTACACCTGATCATTTAGAAAGACATAATGATATAGAAAGTT
ATTTCAAGATAAAGCGTAGCTTACTAGAGAAATCATCCATTCGAATTTATAATTCTGATGATCAATTTTTATCAAGTAAA
AGGAAAGAATTACCTAAAGGTATATGGGTTGGAACAAATCAACAATCCTTCTACTCTGAATATCAGAAATTTTGGATCGA
TCAAAAAGGTTATATATTTGAAGATAAAAAACAACTATTTCATAGTTCTATACTTAAAATACCAGGTAAACATAATTTGC
AAAATCTCTTGCTTGTAATAGCTGCGGCAAGAGAAATAGGGCTTGATGATTCATCAATAGCTAAATCTATAAAATCATTT
AGATCAATCCCCCATAGATTGGAGTATTTAGGAAAGATAAATAATTTAAGCTTTTACAATGACAGCAAAGCTACTAATTT
CGACTCTTCAATAACTGGTTTAAAATCTGTTCCTCATCCAATAATTTTACTAGCTGGTGGTATACAAAAGAAAGGAGATT
TTATGCCTTGGGTGAAACAAATAAAGCAATCTACTAATGGGATAGTTTTATTTGGACTGAGTGCAAATAACTTGAAGGAA
GAATTATTAATGTCTTCTTATACAGGAGAAATAATTATCAAAAAAAATCTAGAAGAAGCAACAATAGCATCTATTAATAT
AGCAAGGGAAACAAATTCAAGAAGCATATTATTATCTCCAGCTTGTGCAAGTTTTGATCAATATAAAAACTATGAAGAGA
GAGGGGACCATTTCAAAAATCTTGTTAAAAAGTATAAATTGATAAAATGA

Upstream 100 bases:

>100_bases
TTATATTAGAGAGTAGTTAAAATAGCATGCAATCAAATTCACTCTAAGGTAACAGCTACCTAAATACGATAAAGCTTTTA
GCATTAAAACCTAAATTAAA

Downstream 100 bases:

>100_bases
TCTATTTTTCAAAACGGATTAGACTTGGTCGGAGATCCACCCTTAGTTTATATACTATGCGTGATAGTTTAGATAAAGAT
ATGCTATGGATAAGTTGAAC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSKQKQSNQIYIVLGLGCSGVSAAKLLKSEGKNVLVLENNSNENLLNISNELKSEGIKVILLDEPLHINNFTPWIESVCS
ITVSPGIDWEHIALKELRGKNINVQGEVELAWERLTHIPSIGITGTNGKTTVTNMLNHILKLNNLNTDMGGNIGKALSKI
ALENMKNNNQELNWLVLELSSFQIEGSPKVAPTIGIWTTFTPDHLERHNDIESYFKIKRSLLEKSSIRIYNSDDQFLSSK
RKELPKGIWVGTNQQSFYSEYQKFWIDQKGYIFEDKKQLFHSSILKIPGKHNLQNLLLVIAAAREIGLDDSSIAKSIKSF
RSIPHRLEYLGKINNLSFYNDSKATNFDSSITGLKSVPHPIILLAGGIQKKGDFMPWVKQIKQSTNGIVLFGLSANNLKE
ELLMSSYTGEIIIKKNLEEATIASINIARETNSRSILLSPACASFDQYKNYEERGDHFKNLVKKYKLIK

Sequences:

>Translated_469_residues
MSKQKQSNQIYIVLGLGCSGVSAAKLLKSEGKNVLVLENNSNENLLNISNELKSEGIKVILLDEPLHINNFTPWIESVCS
ITVSPGIDWEHIALKELRGKNINVQGEVELAWERLTHIPSIGITGTNGKTTVTNMLNHILKLNNLNTDMGGNIGKALSKI
ALENMKNNNQELNWLVLELSSFQIEGSPKVAPTIGIWTTFTPDHLERHNDIESYFKIKRSLLEKSSIRIYNSDDQFLSSK
RKELPKGIWVGTNQQSFYSEYQKFWIDQKGYIFEDKKQLFHSSILKIPGKHNLQNLLLVIAAAREIGLDDSSIAKSIKSF
RSIPHRLEYLGKINNLSFYNDSKATNFDSSITGLKSVPHPIILLAGGIQKKGDFMPWVKQIKQSTNGIVLFGLSANNLKE
ELLMSSYTGEIIIKKNLEEATIASINIARETNSRSILLSPACASFDQYKNYEERGDHFKNLVKKYKLIK
>Mature_468_residues
SKQKQSNQIYIVLGLGCSGVSAAKLLKSEGKNVLVLENNSNENLLNISNELKSEGIKVILLDEPLHINNFTPWIESVCSI
TVSPGIDWEHIALKELRGKNINVQGEVELAWERLTHIPSIGITGTNGKTTVTNMLNHILKLNNLNTDMGGNIGKALSKIA
LENMKNNNQELNWLVLELSSFQIEGSPKVAPTIGIWTTFTPDHLERHNDIESYFKIKRSLLEKSSIRIYNSDDQFLSSKR
KELPKGIWVGTNQQSFYSEYQKFWIDQKGYIFEDKKQLFHSSILKIPGKHNLQNLLLVIAAAREIGLDDSSIAKSIKSFR
SIPHRLEYLGKINNLSFYNDSKATNFDSSITGLKSVPHPIILLAGGIQKKGDFMPWVKQIKQSTNGIVLFGLSANNLKEE
LLMSSYTGEIIIKKNLEEATIASINIARETNSRSILLSPACASFDQYKNYEERGDHFKNLVKKYKLIK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=458, Percent_Identity=28.3842794759825, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 52787; Mature: 52656

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQKQSNQIYIVLGLGCSGVSAAKLLKSEGKNVLVLENNSNENLLNISNELKSEGIKVI
CCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCEEECHHHHHHCCCEEE
LLDEPLHINNFTPWIESVCSITVSPGIDWEHIALKELRGKNINVQGEVELAWERLTHIPS
EECCCCCCCCCCHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCC
IGITGTNGKTTVTNMLNHILKLNNLNTDMGGNIGKALSKIALENMKNNNQELNWLVLELS
CCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEE
SFQIEGSPKVAPTIGIWTTFTPDHLERHNDIESYFKIKRSLLEKSSIRIYNSDDQFLSSK
EEEECCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
RKELPKGIWVGTNQQSFYSEYQKFWIDQKGYIFEDKKQLFHSSILKIPGKHNLQNLLLVI
HHHCCCCEEECCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHH
AAAREIGLDDSSIAKSIKSFRSIPHRLEYLGKINNLSFYNDSKATNFDSSITGLKSVPHP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHCCCCC
IILLAGGIQKKGDFMPWVKQIKQSTNGIVLFGLSANNLKEELLMSSYTGEIIIKKNLEEA
EEEEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCH
TIASINIARETNSRSILLSPACASFDQYKNYEERGDHFKNLVKKYKLIK
HEEEEEEEECCCCCEEEECCCHHCHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQKQSNQIYIVLGLGCSGVSAAKLLKSEGKNVLVLENNSNENLLNISNELKSEGIKVI
CCCCCCCEEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCEEECHHHHHHCCCEEE
LLDEPLHINNFTPWIESVCSITVSPGIDWEHIALKELRGKNINVQGEVELAWERLTHIPS
EECCCCCCCCCCHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCC
IGITGTNGKTTVTNMLNHILKLNNLNTDMGGNIGKALSKIALENMKNNNQELNWLVLELS
CCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEE
SFQIEGSPKVAPTIGIWTTFTPDHLERHNDIESYFKIKRSLLEKSSIRIYNSDDQFLSSK
EEEECCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
RKELPKGIWVGTNQQSFYSEYQKFWIDQKGYIFEDKKQLFHSSILKIPGKHNLQNLLLVI
HHHCCCCEEECCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHH
AAAREIGLDDSSIAKSIKSFRSIPHRLEYLGKINNLSFYNDSKATNFDSSITGLKSVPHP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHCCCCC
IILLAGGIQKKGDFMPWVKQIKQSTNGIVLFGLSANNLKEELLMSSYTGEIIIKKNLEEA
EEEEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCH
TIASINIARETNSRSILLSPACASFDQYKNYEERGDHFKNLVKKYKLIK
HEEEEEEEECCCCCEEEECCCHHCHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA