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The map label for this gene is truB

Identifier: 124026479

GI number: 124026479

Start: 1455466

End: 1456410

Strand: Direct

Name: truB

Synonym: NATL1_17741

Alternate gene names: 124026479

Gene position: 1455466-1456410 (Clockwise)

Preceding gene: 124026477

Following gene: 124026481

Centisome position: 78.05

GC content: 32.06

Gene sequence:

>945_bases
TTGGAAAAGCCCTTTGGATTTGTTGTCATTGATAAGCCTTCTGGGCTTACTTCTCACGATTGTGTAAATAGACTTAGAAA
AGTATTTGGTATCAGAAAAATTGGACATTCTGGAACTCTAGACCCAGCAGTTACTGGAGTACTTCCAATAGCAATAGGTG
ATGCGACAAGATTAATCTCTTATTTACAAGGTTCTAAAGCTTATACAGGAATCATCCAGTTAGGTGCCACTACAAATACT
GATGATATGCAAGGGGAAATTATAGAATCAAAAGCTTGGCCCTTAATTACCCAAAATGATATAAATTATCTACTAGAAAA
CTTTAGAGGTGAGATATTACAAAAGCCTCCTATTTTTTCTAGTGTTCACATTAAAGGTGAGAGAGCTTATAAGAAAGCAA
GAAAAGGAGAAAAATTTGATTTAATTCCTAAAAAAGTTACCATCAACAAATTAAATTTAATAAGCTGGTCTCAAAATAAA
GGTGAGTTATTAGTTGACGTCGACTGCTCAACAGGAACATACATTAGATCCTTGGCTAGAGACATTGGAGATAAAATTGG
ATGCGGTGGTTATTTAAAAAGTTTACGCCGTACAAAAGCATATAATTTCATCGAAAATCATTCAGTAAAACTTCCTGAGA
AATCAGACTTTTACCCAGAGGAAGATAAACCTAAAGTTCTTAATCCTAATATCTTTTTTAAACATCTTTCTTCTTTTGAA
TTAATTTCTGAAGAAGAAATTATTAGCTGGCGTTCAGGAAGAAAGATCAGTTTTCAGAATAATATTAAACGTTTAAAGGT
ATCTAAGAACAATGAAGTTGAAGATAGTTTTATTCATAATAACAATATATTAGTATTGAATAAAGAAAATAAAATCCTTG
GGATCGCTTGCTTAGATGAAAGTTTTGCAATTAAGCCTAAGGTTGTTTTCAATGCAATTGGTTAG

Upstream 100 bases:

>100_bases
TTCATATTATTTGGCTGACTGAACACACTGAGTTATAACTTTAAATGTTCAGGGTTACTTGAACAATATTTTCATGTGAG
CTCAACCTGCAATATCAATT

Downstream 100 bases:

>100_bases
ACTCGTAAAATGTAAATCTTACAATAATCTATAGCTACTAAATATATTCCTAATAGGCTTTTATTTGTACACCTGTATAA
TAATGTTTTAGAATTTTACG

Product: putative tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MEKPFGFVVIDKPSGLTSHDCVNRLRKVFGIRKIGHSGTLDPAVTGVLPIAIGDATRLISYLQGSKAYTGIIQLGATTNT
DDMQGEIIESKAWPLITQNDINYLLENFRGEILQKPPIFSSVHIKGERAYKKARKGEKFDLIPKKVTINKLNLISWSQNK
GELLVDVDCSTGTYIRSLARDIGDKIGCGGYLKSLRRTKAYNFIENHSVKLPEKSDFYPEEDKPKVLNPNIFFKHLSSFE
LISEEEIISWRSGRKISFQNNIKRLKVSKNNEVEDSFIHNNNILVLNKENKILGIACLDESFAIKPKVVFNAIG

Sequences:

>Translated_314_residues
MEKPFGFVVIDKPSGLTSHDCVNRLRKVFGIRKIGHSGTLDPAVTGVLPIAIGDATRLISYLQGSKAYTGIIQLGATTNT
DDMQGEIIESKAWPLITQNDINYLLENFRGEILQKPPIFSSVHIKGERAYKKARKGEKFDLIPKKVTINKLNLISWSQNK
GELLVDVDCSTGTYIRSLARDIGDKIGCGGYLKSLRRTKAYNFIENHSVKLPEKSDFYPEEDKPKVLNPNIFFKHLSSFE
LISEEEIISWRSGRKISFQNNIKRLKVSKNNEVEDSFIHNNNILVLNKENKILGIACLDESFAIKPKVVFNAIG
>Mature_314_residues
MEKPFGFVVIDKPSGLTSHDCVNRLRKVFGIRKIGHSGTLDPAVTGVLPIAIGDATRLISYLQGSKAYTGIIQLGATTNT
DDMQGEIIESKAWPLITQNDINYLLENFRGEILQKPPIFSSVHIKGERAYKKARKGEKFDLIPKKVTINKLNLISWSQNK
GELLVDVDCSTGTYIRSLARDIGDKIGCGGYLKSLRRTKAYNFIENHSVKLPEKSDFYPEEDKPKVLNPNIFFKHLSSFE
LISEEEIISWRSGRKISFQNNIKRLKVSKNNEVEDSFIHNNNILVLNKENKILGIACLDESFAIKPKVVFNAIG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=227, Percent_Identity=36.5638766519824, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI215599015, Length=208, Percent_Identity=31.7307692307692, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI4503337, Length=208, Percent_Identity=31.7307692307692, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI2367200, Length=207, Percent_Identity=34.7826086956522, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17553978, Length=205, Percent_Identity=30.2439024390244, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=202, Percent_Identity=32.1782178217822, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=138, Percent_Identity=36.231884057971, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=33.1632653061224, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=33.1632653061224, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=33.1632653061224, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=33.1632653061224, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=33.1632653061224, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=33.1632653061224, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PROM1 (A2C4C0)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015594.1
- ProteinModelPortal:   A2C4C0
- SMR:   A2C4C0
- STRING:   A2C4C0
- GeneID:   4781243
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_17741
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   TDDMQGE
- ProtClustDB:   CLSK2516626
- BioCyc:   PMAR167555:NATL1_17741-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 35311; Mature: 35311

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: ACT_SITE 41-41

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPFGFVVIDKPSGLTSHDCVNRLRKVFGIRKIGHSGTLDPAVTGVLPIAIGDATRLIS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCEEEEEECCHHHHHH
YLQGSKAYTGIIQLGATTNTDDMQGEIIESKAWPLITQNDINYLLENFRGEILQKPPIFS
HHHCCCCEEEEEEECCCCCCCCCCCEEECCCCCCEEECCHHHHHHHHHHHHHCCCCCCCE
SVHIKGERAYKKARKGEKFDLIPKKVTINKLNLISWSQNKGELLVDVDCSTGTYIRSLAR
EEEECCHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHH
DIGDKIGCGGYLKSLRRTKAYNFIENHSVKLPEKSDFYPEEDKPKVLNPNIFFKHLSSFE
HHHCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCEECCCHHHHHCCCHH
LISEEEIISWRSGRKISFQNNIKRLKVSKNNEVEDSFIHNNNILVLNKENKILGIACLDE
HCCHHHHHHHCCCCEEECCCCCEEEEECCCCCCCHHEEECCCEEEEECCCCEEEEEEECC
SFAIKPKVVFNAIG
CCCCCCEEEEEECC
>Mature Secondary Structure
MEKPFGFVVIDKPSGLTSHDCVNRLRKVFGIRKIGHSGTLDPAVTGVLPIAIGDATRLIS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCEEEEEECCHHHHHH
YLQGSKAYTGIIQLGATTNTDDMQGEIIESKAWPLITQNDINYLLENFRGEILQKPPIFS
HHHCCCCEEEEEEECCCCCCCCCCCEEECCCCCCEEECCHHHHHHHHHHHHHCCCCCCCE
SVHIKGERAYKKARKGEKFDLIPKKVTINKLNLISWSQNKGELLVDVDCSTGTYIRSLAR
EEEECCHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHH
DIGDKIGCGGYLKSLRRTKAYNFIENHSVKLPEKSDFYPEEDKPKVLNPNIFFKHLSSFE
HHHCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCEECCCHHHHHCCCHH
LISEEEIISWRSGRKISFQNNIKRLKVSKNNEVEDSFIHNNNILVLNKENKILGIACLDE
HCCHHHHHHHCCCCEEECCCCCEEEEECCCCCCCHHEEECCCEEEEECCCCEEEEEEECC
SFAIKPKVVFNAIG
CCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA