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The map label for this gene is panB

Identifier: 124026435

GI number: 124026435

Start: 1416833

End: 1417651

Strand: Direct

Name: panB

Synonym: NATL1_17301

Alternate gene names: 124026435

Gene position: 1416833-1417651 (Clockwise)

Preceding gene: 124026434

Following gene: 124026437

Centisome position: 75.98

GC content: 40.17

Gene sequence:

>819_bases
ATGCAAACCACAGATTTGGTTCGTTTTAAAAAGTTGGGCAAACAGATAACTGTATTAACTGCTTGGGATGCTATTTCATC
TTCAATAGTCGAGGCTGCAGGAGCTGATGTTGTTCTAGTGGGAGATTCACTTGGGATGGTTGTCTTGGGACACTCAACGA
CTCTTCCGGTAACACTTGATCAAATGCTTCACCACACAAAGGCTGTTTGTAGAGGTTTTTGTAGACCTCTTTCAAAACAA
CCCTTAGTAGTTTGTGATCTCCCTTTTTTAAGTTATCAATGTGGAGAAGACAAGGCTGTTGAAGCGGCTGGAACTCTCCT
GAAAAACTCCACTGCTTCAGCTGTGAAACTTGAAGGAGCAGAACCTGAGACTTTATTAGTCATTAAGAGACTTATTAGAA
TGGGGATTCCAGTAATGGGGCATCTTGGCCTAACTCCACAGTCAGTTCATCAACTTGGATATAAATCACAAGCTAGGGAT
AAAGATAGTCAAGAAAAAATTTTTAAAGATTCAAAAATGCTTCAAGAATCAGGATGTTTTGCAATCGTTTTAGAACATAT
TCCAGCAGAAATTGCCAGCCGATTAAAGAAACATATAGATATTCCCGTAATTGGCATTGGGGCTGGAAGTGATTGTGATG
GGCAGGTACGAGTTACCGCTGATATTCTTGGCCTTTCAATTGCGCAGCCTCCTTTTGCAAAACCTTTACTTGCAGGACGA
GAACTTTGCATAAATGCCCTAAAAGAATGGATTAATTCTACTGATCTCGATCAAGTGAATCCCACCACGAAAACATCTGA
ATCAAAATCTGATTGCTAA

Upstream 100 bases:

>100_bases
GGCTGCTACCTTCCGGTCCTGACCAGGTTTAAGCGTCACAGCCGCATGGGGCCGAGTCATAAACATTCTAGCAATTCGTA
AACACGTTATTAAGAATTAA

Downstream 100 bases:

>100_bases
TTTGAAACCCCTTAGGATCACTTAAAAAATATCTACCATTGTGTCTTAATAGAAATCCTTCATTTATAGAATTTAGCCAA
AATTTCTCAAGTGATTTTAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MQTTDLVRFKKLGKQITVLTAWDAISSSIVEAAGADVVLVGDSLGMVVLGHSTTLPVTLDQMLHHTKAVCRGFCRPLSKQ
PLVVCDLPFLSYQCGEDKAVEAAGTLLKNSTASAVKLEGAEPETLLVIKRLIRMGIPVMGHLGLTPQSVHQLGYKSQARD
KDSQEKIFKDSKMLQESGCFAIVLEHIPAEIASRLKKHIDIPVIGIGAGSDCDGQVRVTADILGLSIAQPPFAKPLLAGR
ELCINALKEWINSTDLDQVNPTTKTSESKSDC

Sequences:

>Translated_272_residues
MQTTDLVRFKKLGKQITVLTAWDAISSSIVEAAGADVVLVGDSLGMVVLGHSTTLPVTLDQMLHHTKAVCRGFCRPLSKQ
PLVVCDLPFLSYQCGEDKAVEAAGTLLKNSTASAVKLEGAEPETLLVIKRLIRMGIPVMGHLGLTPQSVHQLGYKSQARD
KDSQEKIFKDSKMLQESGCFAIVLEHIPAEIASRLKKHIDIPVIGIGAGSDCDGQVRVTADILGLSIAQPPFAKPLLAGR
ELCINALKEWINSTDLDQVNPTTKTSESKSDC
>Mature_272_residues
MQTTDLVRFKKLGKQITVLTAWDAISSSIVEAAGADVVLVGDSLGMVVLGHSTTLPVTLDQMLHHTKAVCRGFCRPLSKQ
PLVVCDLPFLSYQCGEDKAVEAAGTLLKNSTASAVKLEGAEPETLLVIKRLIRMGIPVMGHLGLTPQSVHQLGYKSQARD
KDSQEKIFKDSKMLQESGCFAIVLEHIPAEIASRLKKHIDIPVIGIGAGSDCDGQVRVTADILGLSIAQPPFAKPLLAGR
ELCINALKEWINSTDLDQVNPTTKTSESKSDC

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=235, Percent_Identity=40.8510638297872, Blast_Score=163, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319653, Length=269, Percent_Identity=34.2007434944238, Blast_Score=152, Evalue=8e-38,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PROM1 (A2C476)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015550.1
- ProteinModelPortal:   A2C476
- SMR:   A2C476
- STRING:   A2C476
- GeneID:   4779162
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_17301
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   PMAR167555:NATL1_17301-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29252; Mature: 29252

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: ACT_SITE 185-185 BINDING 86-86 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTDLVRFKKLGKQITVLTAWDAISSSIVEAAGADVVLVGDSLGMVVLGHSTTLPVTLD
CCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCEEHH
QMLHHTKAVCRGFCRPLSKQPLVVCDLPFLSYQCGEDKAVEAAGTLLKNSTASAVKLEGA
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCC
EPETLLVIKRLIRMGIPVMGHLGLTPQSVHQLGYKSQARDKDSQEKIFKDSKMLQESGCF
CCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCH
AIVLEHIPAEIASRLKKHIDIPVIGIGAGSDCDGQVRVTADILGLSIAQPPFAKPLLAGR
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEHHHHCCCCCCCCHHHHHH
ELCINALKEWINSTDLDQVNPTTKTSESKSDC
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQTTDLVRFKKLGKQITVLTAWDAISSSIVEAAGADVVLVGDSLGMVVLGHSTTLPVTLD
CCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCEEHH
QMLHHTKAVCRGFCRPLSKQPLVVCDLPFLSYQCGEDKAVEAAGTLLKNSTASAVKLEGA
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCC
EPETLLVIKRLIRMGIPVMGHLGLTPQSVHQLGYKSQARDKDSQEKIFKDSKMLQESGCF
CCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCH
AIVLEHIPAEIASRLKKHIDIPVIGIGAGSDCDGQVRVTADILGLSIAQPPFAKPLLAGR
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEHHHHCCCCCCCCHHHHHH
ELCINALKEWINSTDLDQVNPTTKTSESKSDC
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA