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The map label for this gene is folC [H]

Identifier: 124026427

GI number: 124026427

Start: 1407357

End: 1408595

Strand: Direct

Name: folC [H]

Synonym: NATL1_17221

Alternate gene names: 124026427

Gene position: 1407357-1408595 (Clockwise)

Preceding gene: 124026426

Following gene: 124026430

Centisome position: 75.47

GC content: 36.48

Gene sequence:

>1239_bases
ATGTCTGATGAAACTTTCTTAGCACAATCGAATTTCATTTCTGAAAGCAAGAGCAAAGAATATAAAGATATGAATCTGGG
CATAGATCGTATGTCATTGGCTATTAATGCTATGGGAGATCCCTGCAAAAAAACACCTGCTATTCACATAGCAGGAACAA
ATGGGAAAGGCTCTATTGGCGCTTTTATCAATAGTGTTCTTAGCTTAGTAAATATCAAAACTGGAGTTACCACTTCTCCC
CATTTAGTTGATTGGGTCGAGAGGATATGTATCAACAAGACTCCAATATCAAAAGAAGAATTTCAATCATTAAGCCTGTC
TCTTTCTCCAATTGCAAAAAAATATAGCTTGACTCCTTTTGAATGTGTTATTGCAATAGCACTTAAATATTTTACTTTAA
GGGAAGTTGAACTTCTGATCCTTGAAGTAGGGCTTGGAGGTAGACTTGATGCAACAACCGCGCATCAATATAGACCTATT
ATTGCATTTGGAGCTATTGGGCTTGATCATTGTGAATATTTAGGCAATAGTCTAGAAAAAGTAGCTATTGAGAAAGCAGC
TGTAATCACTACAAAGAGCACTGTCATTACAGCCACACAAAATAATATTGTAAAAAGAGTTTTAGAAGAGACTGCCAAGA
GGAAACAAGCAGTCATTCACTGGGTAGATCCAATTCCATTAGACTGGGAACTCGGCTTATCAGGAGTAATACAGAAAGAA
AATGCAGCTGTAGCTAAAGGGGTTATTGAATCTTTAAAAAATATAGGCTGGAATATTTCTGAAGGACAACTTCGAGAAGG
CTTATCTCTTGCTAAATGGCCTGGAAGACTTCAAACAACAAAATGGGAAGGGATGCCCATAGTTGTTGATGGTGCACATA
ATCCTCATGCGGCTAATCAATTATCAATTGAAAGGGACGCATGGACTAATCAAGAAAGTGGAATTATTTGGATACTAGGG
ATTCAAAAAAGAAAAGACATGAAAGGCATTTTGTATAAACTAGTTAGAGAGAAAGATTTAGCTTGGATAGTTCCTGTCCC
TGGCCAACAAAGCTGGTCAAAAGATCAAATTTTAAGTTTTTGCCCTGAATATAAACACCAAATCAAAGAAGCTTTTAGTG
TAGAGGAAGTTTTGTCAACACTTAAAAAACAACATAGATGGCCATCTCCACCACCAATCATTTCAGGATCGCTATATTTA
ATAGGTGACCTTTTTCAAAGGAAAATCTTGACAAGTTAA

Upstream 100 bases:

>100_bases
AACTACTTTTAGTATCTGCTGGTGTAAAAGTATTAAGGATAGTCCCTCCTCTGACTATTACAAAAAAAGAAATCAACGAG
CTTTTATCGAGACTAGACAA

Downstream 100 bases:

>100_bases
ATTGTTTAAAAATATAGCAATTAAATCAATTGACTTTTAGATTCCACCCTTTGAGTTTACCTGGATTGAGAATCCCTTTT
GGATCAAAGTTACTCTTGGC

Product: putative bifunctional dihydrofolate/folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSDETFLAQSNFISESKSKEYKDMNLGIDRMSLAINAMGDPCKKTPAIHIAGTNGKGSIGAFINSVLSLVNIKTGVTTSP
HLVDWVERICINKTPISKEEFQSLSLSLSPIAKKYSLTPFECVIAIALKYFTLREVELLILEVGLGGRLDATTAHQYRPI
IAFGAIGLDHCEYLGNSLEKVAIEKAAVITTKSTVITATQNNIVKRVLEETAKRKQAVIHWVDPIPLDWELGLSGVIQKE
NAAVAKGVIESLKNIGWNISEGQLREGLSLAKWPGRLQTTKWEGMPIVVDGAHNPHAANQLSIERDAWTNQESGIIWILG
IQKRKDMKGILYKLVREKDLAWIVPVPGQQSWSKDQILSFCPEYKHQIKEAFSVEEVLSTLKKQHRWPSPPPIISGSLYL
IGDLFQRKILTS

Sequences:

>Translated_412_residues
MSDETFLAQSNFISESKSKEYKDMNLGIDRMSLAINAMGDPCKKTPAIHIAGTNGKGSIGAFINSVLSLVNIKTGVTTSP
HLVDWVERICINKTPISKEEFQSLSLSLSPIAKKYSLTPFECVIAIALKYFTLREVELLILEVGLGGRLDATTAHQYRPI
IAFGAIGLDHCEYLGNSLEKVAIEKAAVITTKSTVITATQNNIVKRVLEETAKRKQAVIHWVDPIPLDWELGLSGVIQKE
NAAVAKGVIESLKNIGWNISEGQLREGLSLAKWPGRLQTTKWEGMPIVVDGAHNPHAANQLSIERDAWTNQESGIIWILG
IQKRKDMKGILYKLVREKDLAWIVPVPGQQSWSKDQILSFCPEYKHQIKEAFSVEEVLSTLKKQHRWPSPPPIISGSLYL
IGDLFQRKILTS
>Mature_411_residues
SDETFLAQSNFISESKSKEYKDMNLGIDRMSLAINAMGDPCKKTPAIHIAGTNGKGSIGAFINSVLSLVNIKTGVTTSPH
LVDWVERICINKTPISKEEFQSLSLSLSPIAKKYSLTPFECVIAIALKYFTLREVELLILEVGLGGRLDATTAHQYRPII
AFGAIGLDHCEYLGNSLEKVAIEKAAVITTKSTVITATQNNIVKRVLEETAKRKQAVIHWVDPIPLDWELGLSGVIQKEN
AAVAKGVIESLKNIGWNISEGQLREGLSLAKWPGRLQTTKWEGMPIVVDGAHNPHAANQLSIERDAWTNQESGIIWILGI
QKRKDMKGILYKLVREKDLAWIVPVPGQQSWSKDQILSFCPEYKHQIKEAFSVEEVLSTLKKQHRWPSPPPIISGSLYLI
GDLFQRKILTS

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=469, Percent_Identity=25.5863539445629, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI66932984, Length=336, Percent_Identity=27.3809523809524, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1788654, Length=371, Percent_Identity=27.7628032345013, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17553150, Length=316, Percent_Identity=25.9493670886076, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17553148, Length=316, Percent_Identity=25.9493670886076, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71984923, Length=316, Percent_Identity=25.9493670886076, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323760, Length=423, Percent_Identity=31.2056737588652, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6324815, Length=278, Percent_Identity=28.4172661870504, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322718, Length=420, Percent_Identity=23.5714285714286, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641571, Length=269, Percent_Identity=29.7397769516729, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24581568, Length=182, Percent_Identity=32.4175824175824, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45744; Mature: 45612

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDETFLAQSNFISESKSKEYKDMNLGIDRMSLAINAMGDPCKKTPAIHIAGTNGKGSIG
CCCCCEEHHHHHHHHHHCCHHHHHHCCHHHHHEEEECCCCCCCCCCEEEEEECCCCCHHH
AFINSVLSLVNIKTGVTTSPHLVDWVERICINKTPISKEEFQSLSLSLSPIAKKYSLTPF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHCCCCHH
ECVIAIALKYFTLREVELLILEVGLGGRLDATTAHQYRPIIAFGAIGLDHCEYLGNSLEK
HHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHCCCEEEECCCCHHHHHHHCCHHHH
VAIEKAAVITTKSTVITATQNNIVKRVLEETAKRKQAVIHWVDPIPLDWELGLSGVIQKE
HHHHHHHEEEECCEEEEEHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHCCCCCEEECC
NAAVAKGVIESLKNIGWNISEGQLREGLSLAKWPGRLQTTKWEGMPIVVDGAHNPHAANQ
CHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHCCCCCCCCCEECCEEEEEECCCCCCCCCC
LSIERDAWTNQESGIIWILGIQKRKDMKGILYKLVREKDLAWIVPVPGQQSWSKDQILSF
EEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHH
CPEYKHQIKEAFSVEEVLSTLKKQHRWPSPPPIISGSLYLIGDLFQRKILTS
CHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDETFLAQSNFISESKSKEYKDMNLGIDRMSLAINAMGDPCKKTPAIHIAGTNGKGSIG
CCCCEEHHHHHHHHHHCCHHHHHHCCHHHHHEEEECCCCCCCCCCEEEEEECCCCCHHH
AFINSVLSLVNIKTGVTTSPHLVDWVERICINKTPISKEEFQSLSLSLSPIAKKYSLTPF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHCCCCHH
ECVIAIALKYFTLREVELLILEVGLGGRLDATTAHQYRPIIAFGAIGLDHCEYLGNSLEK
HHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHHCCCEEEECCCCHHHHHHHCCHHHH
VAIEKAAVITTKSTVITATQNNIVKRVLEETAKRKQAVIHWVDPIPLDWELGLSGVIQKE
HHHHHHHEEEECCEEEEEHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHCCCCCEEECC
NAAVAKGVIESLKNIGWNISEGQLREGLSLAKWPGRLQTTKWEGMPIVVDGAHNPHAANQ
CHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHCCCCCCCCCEECCEEEEEECCCCCCCCCC
LSIERDAWTNQESGIIWILGIQKRKDMKGILYKLVREKDLAWIVPVPGQQSWSKDQILSF
EEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHH
CPEYKHQIKEAFSVEEVLSTLKKQHRWPSPPPIISGSLYLIGDLFQRKILTS
CHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]