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The map label for this gene is lpd [H]

Identifier: 124026422

GI number: 124026422

Start: 1402176

End: 1403618

Strand: Reverse

Name: lpd [H]

Synonym: NATL1_17171

Alternate gene names: 124026422

Gene position: 1403618-1402176 (Counterclockwise)

Preceding gene: 124026423

Following gene: 124026421

Centisome position: 75.27

GC content: 40.12

Gene sequence:

>1443_bases
GTGAGTGAAATTTCTTTTGACTTTGACTTGATTGTTGTCGGAGCTGGATATGGAGGTTTTGATGCGGCTAAACATGCGGC
AGAGGCTGGCTTGAAAGTGGCGATTGTCGAATCAAGAGATATGGGTGGGACCTGTGTTAACAGAGGCTGTGTCCCCTCTA
AAGCATTACTAGCTGCCAGTGGTAAAGTTCGTGAGCTTGCAGATGTTCCTCATCTTGCTGAATTTGGGATACATTCGGCT
CCGGTTAGATTTGAACGTAAAAAAATAGCCGAACATGCAAAAAATTTAGTCGAGACGATTCGAAAAAATCTAACTAAAAC
ATTGGAAAGATCTGGAGTTGAGATACTTAGGGGAGAAGGACGTTTAGAAGGCAATCAAAAGGTCGGTTTAAGAGAAACTA
ATGGAGTCGATAGAATTTTTTCTGCTAGAGATATTATCTTAGCTACAGGCTCAGACCCTTTTGTACCGCCTGGAATTGAA
ATTGATGGACGAACTGTTTTTACAAGTGATGAGGCTATTAATTTAGAATGGTTGCCAAGATGGATTGCAATAATTGGTAG
TGGCTATATAGGTTTGGAATTCGCAGATATTTATACAGCTTTAGGTTGTGAGGTAACCATGATTGAAGCTCTTGATAAAG
TAATGCCTACTTTTGATCCAGATATCACGAAAATTGCTTCAAGAAACCTAATTGATAAAAGAGATATTGAAACTCGAGCT
GGTGTGTTCGCTACTAAAGTTAAGCCAGGTTGTCCTGTCGAGGTCGAGCTTACAGATGCAAAGAGTCGAGAAGTAATTGA
GGAGTTGCAAGTTGACGCTGTTCTCGTCGCAACAGGCCGAGTACCGTCGACTGAAAATCTAAATCTACAATCCGTTGGAG
TTGAAACAACGAGAGGTTTTATTCCAATTGATGATCAGATGAGAGTATTGGTAAATGAAAAACCAGTTTCTAATCTATGG
GCAGTCGGAGATGTTACGGGTAAGTTGATGTTGGCCCATACCGCTGCAGCGCAAGGGAGTATTGCTGTAGAAAATATTTT
AGGAAAAGCAATAGAAATTGACTACAGAAGTATTCCTGCAGCGACTTTTACTCACCCTGAGATAAGTTCCGTTGGACTCT
CGGAAGAAGAAGCAAAAGATCTAGCGAAAAACGAAGGCTTTGAACTAGGAATTGTTAGAAGTTATTTTAAAGCGAACTCT
AAGGCCTTGGCCGAATTAGAAAGTGATGGAATTATGAAGTTGATTTTTAATAAAGAGACGGGAGAGGTCCTTGGGGCTCA
TATTTATGGATTACATGCGGCTGATCTCATACAAGAGGTTTCTAATGCAATTTCTAGAAGACAACGAGTTATTGACTTAG
CAAAAGAAGTTCATACTCATCCAACATTAAGCGAAGTTGTAGAGGTTGCTTATAAGCAGGCATCTTTACAAATAAAGAAG
TAG

Upstream 100 bases:

>100_bases
ATAGCTCATTAGTTGATTCACTTAATGTGGCATCTGCTGCAGTTCCTCTCTTGTTGGAGCGACAAAGAGTAAAAATGGTT
AAGGGTATCCAAAAAAAATC

Downstream 100 bases:

>100_bases
TAAATCAAATTTTATCTTTATAGATCATTAATTATTTAAATTATGGAAATCAGAAGAAGACCTCCTAACCCAAGTATAAA
AGTAGCTAATTTAGAGTATG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSEISFDFDLIVVGAGYGGFDAAKHAAEAGLKVAIVESRDMGGTCVNRGCVPSKALLAASGKVRELADVPHLAEFGIHSA
PVRFERKKIAEHAKNLVETIRKNLTKTLERSGVEILRGEGRLEGNQKVGLRETNGVDRIFSARDIILATGSDPFVPPGIE
IDGRTVFTSDEAINLEWLPRWIAIIGSGYIGLEFADIYTALGCEVTMIEALDKVMPTFDPDITKIASRNLIDKRDIETRA
GVFATKVKPGCPVEVELTDAKSREVIEELQVDAVLVATGRVPSTENLNLQSVGVETTRGFIPIDDQMRVLVNEKPVSNLW
AVGDVTGKLMLAHTAAAQGSIAVENILGKAIEIDYRSIPAATFTHPEISSVGLSEEEAKDLAKNEGFELGIVRSYFKANS
KALAELESDGIMKLIFNKETGEVLGAHIYGLHAADLIQEVSNAISRRQRVIDLAKEVHTHPTLSEVVEVAYKQASLQIKK

Sequences:

>Translated_480_residues
MSEISFDFDLIVVGAGYGGFDAAKHAAEAGLKVAIVESRDMGGTCVNRGCVPSKALLAASGKVRELADVPHLAEFGIHSA
PVRFERKKIAEHAKNLVETIRKNLTKTLERSGVEILRGEGRLEGNQKVGLRETNGVDRIFSARDIILATGSDPFVPPGIE
IDGRTVFTSDEAINLEWLPRWIAIIGSGYIGLEFADIYTALGCEVTMIEALDKVMPTFDPDITKIASRNLIDKRDIETRA
GVFATKVKPGCPVEVELTDAKSREVIEELQVDAVLVATGRVPSTENLNLQSVGVETTRGFIPIDDQMRVLVNEKPVSNLW
AVGDVTGKLMLAHTAAAQGSIAVENILGKAIEIDYRSIPAATFTHPEISSVGLSEEEAKDLAKNEGFELGIVRSYFKANS
KALAELESDGIMKLIFNKETGEVLGAHIYGLHAADLIQEVSNAISRRQRVIDLAKEVHTHPTLSEVVEVAYKQASLQIKK
>Mature_479_residues
SEISFDFDLIVVGAGYGGFDAAKHAAEAGLKVAIVESRDMGGTCVNRGCVPSKALLAASGKVRELADVPHLAEFGIHSAP
VRFERKKIAEHAKNLVETIRKNLTKTLERSGVEILRGEGRLEGNQKVGLRETNGVDRIFSARDIILATGSDPFVPPGIEI
DGRTVFTSDEAINLEWLPRWIAIIGSGYIGLEFADIYTALGCEVTMIEALDKVMPTFDPDITKIASRNLIDKRDIETRAG
VFATKVKPGCPVEVELTDAKSREVIEELQVDAVLVATGRVPSTENLNLQSVGVETTRGFIPIDDQMRVLVNEKPVSNLWA
VGDVTGKLMLAHTAAAQGSIAVENILGKAIEIDYRSIPAATFTHPEISSVGLSEEEAKDLAKNEGFELGIVRSYFKANSK
ALAELESDGIMKLIFNKETGEVLGAHIYGLHAADLIQEVSNAISRRQRVIDLAKEVHTHPTLSEVVEVAYKQASLQIKK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=477, Percent_Identity=34.1719077568134, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI50301238, Length=481, Percent_Identity=30.977130977131, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI22035672, Length=466, Percent_Identity=28.3261802575107, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI291045266, Length=485, Percent_Identity=27.4226804123711, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI33519430, Length=483, Percent_Identity=26.5010351966874, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI33519428, Length=483, Percent_Identity=26.5010351966874, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI33519426, Length=483, Percent_Identity=26.5010351966874, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI148277065, Length=483, Percent_Identity=26.5010351966874, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI148277071, Length=483, Percent_Identity=26.5010351966874, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI291045268, Length=483, Percent_Identity=24.8447204968944, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=34.698275862069, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=29.8947368421053, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1789915, Length=446, Percent_Identity=27.3542600896861, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=27.7185501066098, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=485, Percent_Identity=34.8453608247423, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI71983419, Length=471, Percent_Identity=30.1486199575372, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=471, Percent_Identity=30.1486199575372, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=490, Percent_Identity=27.9591836734694, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=499, Percent_Identity=24.8496993987976, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=486, Percent_Identity=32.0987654320988, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6325166, Length=482, Percent_Identity=28.0082987551867, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325240, Length=490, Percent_Identity=27.7551020408163, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21358499, Length=478, Percent_Identity=34.9372384937238, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24640553, Length=494, Percent_Identity=28.3400809716599, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24640549, Length=494, Percent_Identity=28.3400809716599, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24640551, Length=494, Percent_Identity=28.3400809716599, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17737741, Length=498, Percent_Identity=26.1044176706827, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 52061; Mature: 51930

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEISFDFDLIVVGAGYGGFDAAKHAAEAGLKVAIVESRDMGGTCVNRGCVPSKALLAAS
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCC
GKVRELADVPHLAEFGIHSAPVRFERKKIAEHAKNLVETIRKNLTKTLERSGVEILRGEG
CCHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
RLEGNQKVGLRETNGVDRIFSARDIILATGSDPFVPPGIEIDGRTVFTSDEAINLEWLPR
CCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCEEECCEEEEECCCEEEHHHHHH
WIAIIGSGYIGLEFADIYTALGCEVTMIEALDKVMPTFDPDITKIASRNLIDKRDIETRA
HHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHCCHHHC
GVFATKVKPGCPVEVELTDAKSREVIEELQVDAVLVATGRVPSTENLNLQSVGVETTRGF
CEEEEECCCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCCCCE
IPIDDQMRVLVNEKPVSNLWAVGDVTGKLMLAHTAAAQGSIAVENILGKAIEIDYRSIPA
EECCCCEEEEECCCCCHHEEEECCCCCEEEEEEEHHCCCCCHHHHHHCCCEEECHHCCCC
ATFTHPEISSVGLSEEEAKDLAKNEGFELGIVRSYFKANSKALAELESDGIMKLIFNKET
CEECCCCCHHCCCCHHHHHHHHHCCCCEEEHHHHHHHCCCHHHHHHCCCCEEEEEEECCC
GEVLGAHIYGLHAADLIQEVSNAISRRQRVIDLAKEVHTHPTLSEVVEVAYKQASLQIKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEECC
>Mature Secondary Structure 
SEISFDFDLIVVGAGYGGFDAAKHAAEAGLKVAIVESRDMGGTCVNRGCVPSKALLAAS
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCC
GKVRELADVPHLAEFGIHSAPVRFERKKIAEHAKNLVETIRKNLTKTLERSGVEILRGEG
CCHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
RLEGNQKVGLRETNGVDRIFSARDIILATGSDPFVPPGIEIDGRTVFTSDEAINLEWLPR
CCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCEEECCEEEEECCCEEEHHHHHH
WIAIIGSGYIGLEFADIYTALGCEVTMIEALDKVMPTFDPDITKIASRNLIDKRDIETRA
HHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHCCHHHC
GVFATKVKPGCPVEVELTDAKSREVIEELQVDAVLVATGRVPSTENLNLQSVGVETTRGF
CEEEEECCCCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCCCCE
IPIDDQMRVLVNEKPVSNLWAVGDVTGKLMLAHTAAAQGSIAVENILGKAIEIDYRSIPA
EECCCCEEEEECCCCCHHEEEECCCCCEEEEEEEHHCCCCCHHHHHHCCCEEECHHCCCC
ATFTHPEISSVGLSEEEAKDLAKNEGFELGIVRSYFKANSKALAELESDGIMKLIFNKET
CEECCCCCHHCCCCHHHHHHHHHCCCCEEEHHHHHHHCCCHHHHHHCCCCEEEEEEECCC
GEVLGAHIYGLHAADLIQEVSNAISRRQRVIDLAKEVHTHPTLSEVVEVAYKQASLQIKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]