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The map label for this gene is thiE [H]

Identifier: 124026399

GI number: 124026399

Start: 1380541

End: 1381593

Strand: Direct

Name: thiE [H]

Synonym: NATL1_16941

Alternate gene names: 124026399

Gene position: 1380541-1381593 (Clockwise)

Preceding gene: 124026397

Following gene: 124026400

Centisome position: 74.03

GC content: 35.52

Gene sequence:

>1053_bases
ATGAAATCAATACCTGTCACCCCTCCTTCTGATAATCGTATTGCTCAATTAATTGACGCGAACCTTGATCGCGCTAGAGA
GGGGCTTAGAGTTATGGAAGATTGGTGCAGATTTGGTTTAAAGAGGAGTGATTTTTCGATTCAAATCAAAGATTGGAGGC
AACAATTAGGAGTACATCACCACAATATTTATCGAAAAGAAAGGCTTACATCCATCGATCCAGCTATGGGCATTTCACAT
CCGTTACAAACAGTCAGATCAACCCCAGAGGATGTATTTATTGCAAACTCATCCAGAGTTCAAGAAGCCCTAAGAGTAAT
AGAGGAATTCACTCGAAAAACAGATCCGAATCTTTGTGAAATAGCCAGCAAAATTAGATACGAAACTTATGAAATCGAGA
TAAAGGTGCTTAAATCGACAGAAGGCGTAAATAAAAGAGAAACCTTAAAATATTGTTCGGTATACTTAATAACCTCAAAC
AGGAGAGATATAGAAGAGGTTGTTCTTCATGCTCTAAAAGCTGGTGTAAAAATAGTTCAATATAGAGAGAAATTTTTAAA
CGATAATGAAAAAATTTCACAAGCTAAATGTTTAGCCTCTCTTTGTAAAAAATTCAATTCACTTTTTATAGTCAATGACC
GTATTGATATCGCACTCGCCGTTGACGCAGATGGAATTCATTTGGGACAAGAAGATATGCCAACAAAAATCGCGAGACAA
CTACTAGGGGCTGAAAAAATCATTGGCAGAAGCACGCACTGTCTTGAAGACATCAAAAATGCCGAAGGAGAAGGCTGTGA
TTATATTGGTATAGGGCCAATATTTCCTTCTGAAACAAAAAAGCAACTAAATCCAATTGGAATTGACAACCTAAAAAAAG
GATTAAGTGAAACTATCCTTCCTGCTTTTGCTATTGGGGGAATTAATAAATCAAATATCACAAAATTAAATCAGATAAAT
AATCTTCGCATTGCTGTCTCAAATGCAATCATTAATTCAAATGATCCCTTTTCAACAACTGAAGAGCTTATCAAATTTCT
AATATGCAATTAA

Upstream 100 bases:

>100_bases
CCTCCTAACAAAAAAACACCCCAAAGAAGCACAGTCATATTGTTCTCAGAATCATTTTCCACAATAAAATTTAAATACAA
ATCATCAAATAAAGGATGAC

Downstream 100 bases:

>100_bases
AAATTAACGGCGAAATTAAAACCATTAACAACTCCAATGAAGAATTACTGGAAGCTTTGCTTGAAAACTTAGGTTACAAA
CCTCAGCTAGTTGTAGTTGA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKSIPVTPPSDNRIAQLIDANLDRAREGLRVMEDWCRFGLKRSDFSIQIKDWRQQLGVHHHNIYRKERLTSIDPAMGISH
PLQTVRSTPEDVFIANSSRVQEALRVIEEFTRKTDPNLCEIASKIRYETYEIEIKVLKSTEGVNKRETLKYCSVYLITSN
RRDIEEVVLHALKAGVKIVQYREKFLNDNEKISQAKCLASLCKKFNSLFIVNDRIDIALAVDADGIHLGQEDMPTKIARQ
LLGAEKIIGRSTHCLEDIKNAEGEGCDYIGIGPIFPSETKKQLNPIGIDNLKKGLSETILPAFAIGGINKSNITKLNQIN
NLRIAVSNAIINSNDPFSTTEELIKFLICN

Sequences:

>Translated_350_residues
MKSIPVTPPSDNRIAQLIDANLDRAREGLRVMEDWCRFGLKRSDFSIQIKDWRQQLGVHHHNIYRKERLTSIDPAMGISH
PLQTVRSTPEDVFIANSSRVQEALRVIEEFTRKTDPNLCEIASKIRYETYEIEIKVLKSTEGVNKRETLKYCSVYLITSN
RRDIEEVVLHALKAGVKIVQYREKFLNDNEKISQAKCLASLCKKFNSLFIVNDRIDIALAVDADGIHLGQEDMPTKIARQ
LLGAEKIIGRSTHCLEDIKNAEGEGCDYIGIGPIFPSETKKQLNPIGIDNLKKGLSETILPAFAIGGINKSNITKLNQIN
NLRIAVSNAIINSNDPFSTTEELIKFLICN
>Mature_350_residues
MKSIPVTPPSDNRIAQLIDANLDRAREGLRVMEDWCRFGLKRSDFSIQIKDWRQQLGVHHHNIYRKERLTSIDPAMGISH
PLQTVRSTPEDVFIANSSRVQEALRVIEEFTRKTDPNLCEIASKIRYETYEIEIKVLKSTEGVNKRETLKYCSVYLITSN
RRDIEEVVLHALKAGVKIVQYREKFLNDNEKISQAKCLASLCKKFNSLFIVNDRIDIALAVDADGIHLGQEDMPTKIARQ
LLGAEKIIGRSTHCLEDIKNAEGEGCDYIGIGPIFPSETKKQLNPIGIDNLKKGLSETILPAFAIGGINKSNITKLNQIN
NLRIAVSNAIINSNDPFSTTEELIKFLICN

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=177, Percent_Identity=29.9435028248588, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=190, Percent_Identity=36.3157894736842, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 39516; Mature: 39516

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIPVTPPSDNRIAQLIDANLDRAREGLRVMEDWCRFGLKRSDFSIQIKDWRQQLGVHH
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCHH
HNIYRKERLTSIDPAMGISHPLQTVRSTPEDVFIANSSRVQEALRVIEEFTRKTDPNLCE
HHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHH
IASKIRYETYEIEIKVLKSTEGVNKRETLKYCSVYLITSNRRDIEEVVLHALKAGVKIVQ
HHHHHCEEEEEEEEEEEECCCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHH
YREKFLNDNEKISQAKCLASLCKKFNSLFIVNDRIDIALAVDADGIHLGQEDMPTKIARQ
HHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCEEEEEEECCCCCCCCCCCCHHHHHHH
LLGAEKIIGRSTHCLEDIKNAEGEGCDYIGIGPIFPSETKKQLNPIGIDNLKKGLSETIL
HHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHH
PAFAIGGINKSNITKLNQINNLRIAVSNAIINSNDPFSTTEELIKFLICN
HHHHHCCCCHHHCHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKSIPVTPPSDNRIAQLIDANLDRAREGLRVMEDWCRFGLKRSDFSIQIKDWRQQLGVHH
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCHH
HNIYRKERLTSIDPAMGISHPLQTVRSTPEDVFIANSSRVQEALRVIEEFTRKTDPNLCE
HHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHH
IASKIRYETYEIEIKVLKSTEGVNKRETLKYCSVYLITSNRRDIEEVVLHALKAGVKIVQ
HHHHHCEEEEEEEEEEEECCCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHH
YREKFLNDNEKISQAKCLASLCKKFNSLFIVNDRIDIALAVDADGIHLGQEDMPTKIARQ
HHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCEEEEEEECCCCCCCCCCCCHHHHHHH
LLGAEKIIGRSTHCLEDIKNAEGEGCDYIGIGPIFPSETKKQLNPIGIDNLKKGLSETIL
HHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHH
PAFAIGGINKSNITKLNQINNLRIAVSNAIINSNDPFSTTEELIKFLICN
HHHHHCCCCHHHCHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA