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The map label for this gene is ydfG [C]

Identifier: 124026273

GI number: 124026273

Start: 1273099

End: 1274001

Strand: Direct

Name: ydfG [C]

Synonym: NATL1_15671

Alternate gene names: 124026273

Gene position: 1273099-1274001 (Clockwise)

Preceding gene: 124026272

Following gene: 124026278

Centisome position: 68.27

GC content: 34.55

Gene sequence:

>903_bases
ATGGTTAGATTAAATGAAATCAAGATGCAAGACGGAAAAGTATTCTTAATTACCGGAGCCAATAGTGGACTTGGCTATGA
AACATCAAAATTCCTTTTAGAAAGGGGAGCAACAGTAATCATGTCTTGCAGAGACTTGATCAAAGGAGAGAAAGCCAAAC
AAGAACTTTTAAAATTTAATTTTTCTGGAAAGATCGAATTAGTTGAATTAGATTTATCCGATTTAATAAACGTTAAAAAA
TTTGCTGAATCTATAAAAAATAAATTTGATTACTTAGATGTTTTAATCAATAATGCTGGGATAATGGCTCCACCAAAAAC
TTTTAGCAAGCAAGGTTTTGAAATACAGTTTGCGGTTAATCATCTTGCACATATGTTTTTAACGTTAGAACTATTACCCA
TGCTTGAAGAAAAAAATAATTCTAGAGTTGTCACAGTAACCTCAGGTGTCCAATATTTTGGAAAGATTCAGTGGGCAGAT
TTACAAGGAAATCTTAAATACGATCGTTGGGCTTCATATGCGCAGAGCAAGCTTGCAAACGTAATGTTTGGCTTAGAACT
TGATTCAAAACTTAAAGAAAGCAATTCAAAAACTTCTTCACTACTAGCTCATCCAGGATTTGCACGTACAAATTTACAGC
CAAAGTCTGTTGAGGCTAATCAGTCATGGCAAGAAGAACTTGCTTATAAATTGATGGATCCCATGTTTCAAAGCGCGAAA
ATGGGTGCATTGCCTCAAATAACTGCCGCCACATTAACTAGTGCTTCGGGAGGAGAACAATATGGACCTAGATTTAGCTT
CAGAGGGTATCCAAAAATATGTAGCAATGCTCCAAAAGCATTAAATCAAACTTCAAGAAAAAAATTGTGGGAAATAAGCG
AAAAACTTATAAAAGGTGTTTGA

Upstream 100 bases:

>100_bases
AAATCTTATTAATTTTATTAAATCAATACAAACAGATCATCCAGGTCTACCACACTTAAATTAAATCATAATTATAGTAA
ATAAACATTTTCAAAGGGTT

Downstream 100 bases:

>100_bases
TATCCTTTCAAAACAAAGGAAGTAATTCTTCTCCTTTGTCACATATTTCTTGTTGTGAAGGGTACTCTGTTTTTCCAGTC
CAATGAATTAAGCCTTTTCT

Product: short-chain dehydrogenase/reductase

Products: protochlorophyllide; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MVRLNEIKMQDGKVFLITGANSGLGYETSKFLLERGATVIMSCRDLIKGEKAKQELLKFNFSGKIELVELDLSDLINVKK
FAESIKNKFDYLDVLINNAGIMAPPKTFSKQGFEIQFAVNHLAHMFLTLELLPMLEEKNNSRVVTVTSGVQYFGKIQWAD
LQGNLKYDRWASYAQSKLANVMFGLELDSKLKESNSKTSSLLAHPGFARTNLQPKSVEANQSWQEELAYKLMDPMFQSAK
MGALPQITAATLTSASGGEQYGPRFSFRGYPKICSNAPKALNQTSRKKLWEISEKLIKGV

Sequences:

>Translated_300_residues
MVRLNEIKMQDGKVFLITGANSGLGYETSKFLLERGATVIMSCRDLIKGEKAKQELLKFNFSGKIELVELDLSDLINVKK
FAESIKNKFDYLDVLINNAGIMAPPKTFSKQGFEIQFAVNHLAHMFLTLELLPMLEEKNNSRVVTVTSGVQYFGKIQWAD
LQGNLKYDRWASYAQSKLANVMFGLELDSKLKESNSKTSSLLAHPGFARTNLQPKSVEANQSWQEELAYKLMDPMFQSAK
MGALPQITAATLTSASGGEQYGPRFSFRGYPKICSNAPKALNQTSRKKLWEISEKLIKGV
>Mature_300_residues
MVRLNEIKMQDGKVFLITGANSGLGYETSKFLLERGATVIMSCRDLIKGEKAKQELLKFNFSGKIELVELDLSDLINVKK
FAESIKNKFDYLDVLINNAGIMAPPKTFSKQGFEIQFAVNHLAHMFLTLELLPMLEEKNNSRVVTVTSGVQYFGKIQWAD
LQGNLKYDRWASYAQSKLANVMFGLELDSKLKESNSKTSSLLAHPGFARTNLQPKSVEANQSWQEELAYKLMDPMFQSAK
MGALPQITAATLTSASGGEQYGPRFSFRGYPKICSNAPKALNQTSRKKLWEISEKLIKGV

Specific function: Unknown

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI186928839, Length=289, Percent_Identity=36.3321799307958, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI10190746, Length=305, Percent_Identity=37.0491803278689, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI166795268, Length=287, Percent_Identity=35.5400696864112, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI7706523, Length=298, Percent_Identity=33.5570469798658, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI225579078, Length=290, Percent_Identity=33.7931034482759, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI193804850, Length=255, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI146231950, Length=287, Percent_Identity=30.6620209059233, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI19923931, Length=254, Percent_Identity=32.2834645669291, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI13375997, Length=160, Percent_Identity=36.875, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI72534732, Length=160, Percent_Identity=36.875, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI20149619, Length=231, Percent_Identity=27.7056277056277, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI210032110, Length=243, Percent_Identity=26.3374485596708, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4502599, Length=139, Percent_Identity=32.3741007194245, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI142976729, Length=249, Percent_Identity=25.3012048192771, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17532805, Length=317, Percent_Identity=33.1230283911672, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17505416, Length=217, Percent_Identity=35.4838709677419, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193207620, Length=212, Percent_Identity=35.8490566037736, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17558006, Length=219, Percent_Identity=32.8767123287671, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17532791, Length=252, Percent_Identity=30.952380952381, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32565532, Length=141, Percent_Identity=39.0070921985816, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71992402, Length=214, Percent_Identity=34.1121495327103, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI72001364, Length=212, Percent_Identity=31.1320754716981, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535059, Length=250, Percent_Identity=28, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115533172, Length=186, Percent_Identity=29.0322580645161, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115533174, Length=186, Percent_Identity=29.0322580645161, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508895, Length=203, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324820, Length=228, Percent_Identity=30.2631578947368, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322227, Length=210, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323882, Length=231, Percent_Identity=27.7056277056277, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19921754, Length=203, Percent_Identity=46.3054187192118, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI22024069, Length=241, Percent_Identity=39.8340248962656, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45554248, Length=289, Percent_Identity=36.3321799307958, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24640117, Length=289, Percent_Identity=36.3321799307958, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24586328, Length=292, Percent_Identity=34.9315068493151, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI28573296, Length=203, Percent_Identity=42.8571428571429, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI161076371, Length=241, Percent_Identity=37.344398340249, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI20129347, Length=296, Percent_Identity=30.4054054054054, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24656154, Length=260, Percent_Identity=34.6153846153846, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922628, Length=260, Percent_Identity=34.6153846153846, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24647946, Length=306, Percent_Identity=32.3529411764706, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45553409, Length=299, Percent_Identity=32.1070234113712, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24647948, Length=299, Percent_Identity=32.1070234113712, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24647931, Length=227, Percent_Identity=29.0748898678414, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21358495, Length=206, Percent_Identity=27.6699029126214, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.33

Molecular weight: Translated: 33596; Mature: 33596

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLNEIKMQDGKVFLITGANSGLGYETSKFLLERGATVIMSCRDLIKGEKAKQELLKFN
CCCCCCEEECCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCC
FSGKIELVELDLSDLINVKKFAESIKNKFDYLDVLINNAGIMAPPKTFSKQGFEIQFAVN
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEHHHH
HLAHMFLTLELLPMLEEKNNSRVVTVTSGVQYFGKIQWADLQGNLKYDRWASYAQSKLAN
HHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCEEEEECCCCCCHHHHHHHHHHHHHH
VMFGLELDSKLKESNSKTSSLLAHPGFARTNLQPKSVEANQSWQEELAYKLMDPMFQSAK
HHHCCCHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
MGALPQITAATLTSASGGEQYGPRFSFRGYPKICSNAPKALNQTSRKKLWEISEKLIKGV
CCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVRLNEIKMQDGKVFLITGANSGLGYETSKFLLERGATVIMSCRDLIKGEKAKQELLKFN
CCCCCCEEECCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCC
FSGKIELVELDLSDLINVKKFAESIKNKFDYLDVLINNAGIMAPPKTFSKQGFEIQFAVN
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEHHHH
HLAHMFLTLELLPMLEEKNNSRVVTVTSGVQYFGKIQWADLQGNLKYDRWASYAQSKLAN
HHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCEEEEECCCCCCHHHHHHHHHHHHHH
VMFGLELDSKLKESNSKTSSLLAHPGFARTNLQPKSVEANQSWQEELAYKLMDPMFQSAK
HHHCCCHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
MGALPQITAATLTSASGGEQYGPRFSFRGYPKICSNAPKALNQTSRKKLWEISEKLIKGV
CCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: chlorophyllide a; NADP+

Specific reaction: chlorophyllide a + NADP+ = protochlorophyllide + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]