Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 124026239

GI number: 124026239

Start: 1247979

End: 1248443

Strand: Direct

Name: ruvC

Synonym: NATL1_15331

Alternate gene names: 124026239

Gene position: 1247979-1248443 (Clockwise)

Preceding gene: 124026238

Following gene: 124026240

Centisome position: 66.93

GC content: 36.99

Gene sequence:

>465_bases
GTGCGAATAATAGGAATTGATCCAGGGTTAGCAAGGGTAGGTTACGGAATCATTGATGAAATAGAGGGAAAGAAAATAAT
GATTGATTGCGGAATTATAGAGACAAAATCAACAAAGAAAGAGGAAGAAAGACTTGTAGAAATTTCAAATGATTTAAGCT
CGATAATAAAAAAATGGAATCCCAATTCTGCAGCGGTAGAAAAGTTTTTTTTCTACCGCTCTAGCACAACGATTAGTGTT
GTTCAGGCTCGTGGAGTGATAATGATGACTTTAGGAAAGTACAAGCTTCCGATTCAAGAATTCCCCCCAATGCAAATCAA
ACTTGCTGTTACTGGTTATGGTCATTCCGACAAGAATGAGGTGTTAAATTGTGTTATGCATGAACTCAGTATCACTTCAC
CACCAAAGCCAGATGATGCTGCTGATGCTCTAGCAATAGCATTAACTGGAATTTATCTTAAATAA

Upstream 100 bases:

>100_bases
AGTTGATTCTGGAGATAGAGTAATAAAAGCTTTTTGCAAAGTATTTGAAAGGAATGAAAGTGATGACGTATCTGAATTCG
AACTAGCCACCGCTAACTAA

Downstream 100 bases:

>100_bases
TCTAATCGGTAATTAAAATATACTATTCATGAAAAATATTGATGATGAGGTTAAATCAAAAAAAAATAATTTAAGAAAAG
AATATACTTTGATCCGTAAT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MRIIGIDPGLARVGYGIIDEIEGKKIMIDCGIIETKSTKKEEERLVEISNDLSSIIKKWNPNSAAVEKFFFYRSSTTISV
VQARGVIMMTLGKYKLPIQEFPPMQIKLAVTGYGHSDKNEVLNCVMHELSITSPPKPDDAADALAIALTGIYLK

Sequences:

>Translated_154_residues
MRIIGIDPGLARVGYGIIDEIEGKKIMIDCGIIETKSTKKEEERLVEISNDLSSIIKKWNPNSAAVEKFFFYRSSTTISV
VQARGVIMMTLGKYKLPIQEFPPMQIKLAVTGYGHSDKNEVLNCVMHELSITSPPKPDDAADALAIALTGIYLK
>Mature_154_residues
MRIIGIDPGLARVGYGIIDEIEGKKIMIDCGIIETKSTKKEEERLVEISNDLSSIIKKWNPNSAAVEKFFFYRSSTTISV
VQARGVIMMTLGKYKLPIQEFPPMQIKLAVTGYGHSDKNEVLNCVMHELSITSPPKPDDAADALAIALTGIYLK

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=135, Percent_Identity=30.3703703703704, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_PROM1 (A2C3N0)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015355.1
- ProteinModelPortal:   A2C3N0
- SMR:   A2C3N0
- STRING:   A2C3N0
- GeneID:   4780966
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_15331
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- BioCyc:   PMAR167555:NATL1_15331-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17030; Mature: 17030

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIGIDPGLARVGYGIIDEIEGKKIMIDCGIIETKSTKKEEERLVEISNDLSSIIKKWN
CEEEECCCCHHHHCCCCCCCCCCCEEEEEECEEECCCCCHHHHHHHHHHHHHHHHHHHCC
PNSAAVEKFFFYRSSTTISVVQARGVIMMTLGKYKLPIQEFPPMQIKLAVTGYGHSDKNE
CCHHHHHHHHEEECCCEEEEEEECCEEEEECCCEECCHHHCCCCEEEEEEEECCCCCHHH
VLNCVMHELSITSPPKPDDAADALAIALTGIYLK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEECC
>Mature Secondary Structure
MRIIGIDPGLARVGYGIIDEIEGKKIMIDCGIIETKSTKKEEERLVEISNDLSSIIKKWN
CEEEECCCCHHHHCCCCCCCCCCCEEEEEECEEECCCCCHHHHHHHHHHHHHHHHHHHCC
PNSAAVEKFFFYRSSTTISVVQARGVIMMTLGKYKLPIQEFPPMQIKLAVTGYGHSDKNE
CCHHHHHHHHEEECCCEEEEEEECCEEEEECCCEECCHHHCCCCEEEEEEEECCCCCHHH
VLNCVMHELSITSPPKPDDAADALAIALTGIYLK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA