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The map label for this gene is rsuA [H]

Identifier: 124026115

GI number: 124026115

Start: 1146210

End: 1146932

Strand: Direct

Name: rsuA [H]

Synonym: NATL1_14081

Alternate gene names: 124026115

Gene position: 1146210-1146932 (Clockwise)

Preceding gene: 124026114

Following gene: 124026128

Centisome position: 61.47

GC content: 32.37

Gene sequence:

>723_bases
ATGGCTACCAGAATTAATAAATATTTAAGTGAAGTCGGTTACTGTTCTAGAAGAGTCGCAGATAGACTAATTGAAGAAGG
AAAAGTAGTCATTAATGGTAAAATTCCAGAAAAGGGTACGAAGGTAGAGGAAGGTGATCAAGTAAAAGTTGACGGTCAAA
GAATAGAAAAATCAACGAAACAAAAAAATATATACTTAGCCTTTAATAAACCTGTTGGGATTGTTTGCACAACCGATAGA
AGAGTAGAACACGACAACATTATAGATTTCATTAAATATCCTACAAGAATTTTTCCTATTGGAAGATTGGATAAGCCCAG
TGAGGGCCTAATTTTTTTAACTAACGATGGAGATATCGTAAATAAAATACTCAGAGCAAGAAATAATCATGAAAAAGAAT
ACATCGTAAGCGTTAATCGTCCGATTAATAGAGACTTTATTCAAAGTATGAGTAATGGAGTTGAAATACTAGAAACCATA
ACTAAGAATTGTTTCGTAAAACAATTGGGGGCGAAAAAATTTAAAATCATACTCACTCAAGGACTTAACCGTCAGATTAG
GAGAATGTGTGAGTCCTTAGGCTACAGAGTAGAATCATTAAAGAGGGTAAGAATTATGAATATTAAGTTAGACGTACCAA
TTGGAAAATATCGCGAATTTACCAAAGAAGAACTTTTAGAATTAAATGGATTACTCGAAAACTCTTCAAAAACATTTGAC
TAA

Upstream 100 bases:

>100_bases
AATATCGTCAAATTTTGCCGAGTTTGTCATGACAAATAAAGCAATATTTCTCTGCAATTTGTCCGTATATTTAAAATAGA
TAAAAGGTAACTTTGACAAA

Downstream 100 bases:

>100_bases
TCAAATATCCCCTCTTTTTTAGCTAAATAAGATTACGTTTGTTTTTTCTGTCTGCTTTTGTTGCGGACAGAATTTAATCA
ATAAAAAAGACAGGCTGGCA

Product: putative pseudouridylate synthase specific to ribosomal small subunit

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MATRINKYLSEVGYCSRRVADRLIEEGKVVINGKIPEKGTKVEEGDQVKVDGQRIEKSTKQKNIYLAFNKPVGIVCTTDR
RVEHDNIIDFIKYPTRIFPIGRLDKPSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINRDFIQSMSNGVEILETI
TKNCFVKQLGAKKFKIILTQGLNRQIRRMCESLGYRVESLKRVRIMNIKLDVPIGKYREFTKEELLELNGLLENSSKTFD

Sequences:

>Translated_240_residues
MATRINKYLSEVGYCSRRVADRLIEEGKVVINGKIPEKGTKVEEGDQVKVDGQRIEKSTKQKNIYLAFNKPVGIVCTTDR
RVEHDNIIDFIKYPTRIFPIGRLDKPSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINRDFIQSMSNGVEILETI
TKNCFVKQLGAKKFKIILTQGLNRQIRRMCESLGYRVESLKRVRIMNIKLDVPIGKYREFTKEELLELNGLLENSSKTFD
>Mature_239_residues
ATRINKYLSEVGYCSRRVADRLIEEGKVVINGKIPEKGTKVEEGDQVKVDGQRIEKSTKQKNIYLAFNKPVGIVCTTDRR
VEHDNIIDFIKYPTRIFPIGRLDKPSEGLIFLTNDGDIVNKILRARNNHEKEYIVSVNRPINRDFIQSMSNGVEILETIT
KNCFVKQLGAKKFKIILTQGLNRQIRRMCESLGYRVESLKRVRIMNIKLDVPIGKYREFTKEELLELNGLLENSSKTFD

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=57.2033898305085, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=28.82096069869, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787524, Length=250, Percent_Identity=29.6, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87081838, Length=186, Percent_Identity=26.8817204301075, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27693; Mature: 27561

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRINKYLSEVGYCSRRVADRLIEEGKVVINGKIPEKGTKVEEGDQVKVDGQRIEKSTK
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHH
QKNIYLAFNKPVGIVCTTDRRVEHDNIIDFIKYPTRIFPIGRLDKPSEGLIFLTNDGDIV
HCEEEEEECCCEEEEECCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCHHH
NKILRARNNHEKEYIVSVNRPINRDFIQSMSNGVEILETITKNCFVKQLGAKKFKIILTQ
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
GLNRQIRRMCESLGYRVESLKRVRIMNIKLDVPIGKYREFTKEELLELNGLLENSSKTFD
CCCHHHHHHHHHHCCHHHHHCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ATRINKYLSEVGYCSRRVADRLIEEGKVVINGKIPEKGTKVEEGDQVKVDGQRIEKSTK
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHH
QKNIYLAFNKPVGIVCTTDRRVEHDNIIDFIKYPTRIFPIGRLDKPSEGLIFLTNDGDIV
HCEEEEEECCCEEEEECCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCHHH
NKILRARNNHEKEYIVSVNRPINRDFIQSMSNGVEILETITKNCFVKQLGAKKFKIILTQ
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
GLNRQIRRMCESLGYRVESLKRVRIMNIKLDVPIGKYREFTKEELLELNGLLENSSKTFD
CCCHHHHHHHHHHCCHHHHHCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]