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The map label for this gene is purT

Identifier: 124025937

GI number: 124025937

Start: 1071892

End: 1073055

Strand: Reverse

Name: purT

Synonym: NATL1_12301

Alternate gene names: 124025937

Gene position: 1073055-1071892 (Counterclockwise)

Preceding gene: 124025938

Following gene: 124025936

Centisome position: 57.54

GC content: 35.22

Gene sequence:

>1164_bases
ATGAATGTTTTTCCAAAGAAAATAATGCTTTTAGGTAGTGGAGAATTGGGGAAAGAGGTAGCAATAGCAGCAAAGAGATT
AGGTTGTTATGTTATTGCATGTGATAGGTACAATGATGCTCCAGCAATGCAAATAGCTGATCAATTTAATGTATTTAATA
TGAATAATGGTAGTGAATTAAAAGAAGTGATATATAAATGTAATCCTGATATTATTATTCCTGAAATTGAAGCTCTTGCA
GTTGATGTACTAAAGGAAATCGAACAAAAGATAACAGTAATACCCAACTCAAGAGCTACTGCAACAACAATGAATAGAGA
TAAAATTAGAGATTTGGCTTCTAACGAATTAAATATAAGAACTGCAAAATTTAGTTATGCGGTTAATCAATCTGAGCTTG
ATTTGCATGCAGAGACGATTGGGTATCCTTTATTAATTAAGCCAGTCATGAGTTCTTCGGGTAAAGGGCAAAGCTTAGTT
AAAAATAAGAATGAATTAGCACAGGCTTGGAATTTGGCTATTGAAAAATCAAGAGGTAAATCAAATAAAATCATATTAGA
AGAATTTATTGATTTTGATTTGGAAATTACTTTATTAACTATTAGACAATCTAATGGTAAGACATTATTTTGTGCTCCTA
TAGGACATGAGCAAAAAAATGGAGATTATCAATGTAGTTGGCAACCAGCTGAATTAACTGAAAGTGTCTTAGAGAAGGCT
CAACAGATTGCCAAACGTGTTACCGATAATCTTGGTGGAGTAGGTTTGTTTGGTGTTGAATTTTTTATTAAGGGTGAGGA
GGTGATTTTTTCAGAGCTATCACCAAGACCGCATGACACAGGCTTAGTAACTTTGATTAGCCAAAATTTAAATGAGTTTG
AATTACACCTTAGAGCTGTTTTAGGTATCCCAATCCCAGAAATAGTTTGCCATGAAGCTTCTGCAAGTAGAGTGATTTTG
GCTTCCATGGAAACCACAGACGTTGCTTTTACGGGACTTGAGCAAGCCTTAAGCCAATCGAATACAAATGTATTCATGTT
TGGCAAGCCGAGCTCAACGGAAGGTCGTCGAATGGGAGTAGCCGTAGCAAAGGCTGAAACAATCGATGAGGCAAGAATTA
AAGCCGACAATGCAGCGCAATCAATCCAATTCATAAATGAATAA

Upstream 100 bases:

>100_bases
TTCAATCACCAGTGCTTGTTAATTTCTACTTCTTTTTCTAATAATTGATTCTGTTTTAGAGATATTATATTTATATATTT
TTTTTGACCTATACATAATT

Downstream 100 bases:

>100_bases
TATGAATTCAGGTTGTTTATCTGATTTCAATAGGTGCAAGTCGATTTGGTTTCTATTTTTGACTGAGTTGAATTGGAATC
CTAATGCAATCAACCCTCTC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MNVFPKKIMLLGSGELGKEVAIAAKRLGCYVIACDRYNDAPAMQIADQFNVFNMNNGSELKEVIYKCNPDIIIPEIEALA
VDVLKEIEQKITVIPNSRATATTMNRDKIRDLASNELNIRTAKFSYAVNQSELDLHAETIGYPLLIKPVMSSSGKGQSLV
KNKNELAQAWNLAIEKSRGKSNKIILEEFIDFDLEITLLTIRQSNGKTLFCAPIGHEQKNGDYQCSWQPAELTESVLEKA
QQIAKRVTDNLGGVGLFGVEFFIKGEEVIFSELSPRPHDTGLVTLISQNLNEFELHLRAVLGIPIPEIVCHEASASRVIL
ASMETTDVAFTGLEQALSQSNTNVFMFGKPSSTEGRRMGVAVAKAETIDEARIKADNAAQSIQFINE

Sequences:

>Translated_387_residues
MNVFPKKIMLLGSGELGKEVAIAAKRLGCYVIACDRYNDAPAMQIADQFNVFNMNNGSELKEVIYKCNPDIIIPEIEALA
VDVLKEIEQKITVIPNSRATATTMNRDKIRDLASNELNIRTAKFSYAVNQSELDLHAETIGYPLLIKPVMSSSGKGQSLV
KNKNELAQAWNLAIEKSRGKSNKIILEEFIDFDLEITLLTIRQSNGKTLFCAPIGHEQKNGDYQCSWQPAELTESVLEKA
QQIAKRVTDNLGGVGLFGVEFFIKGEEVIFSELSPRPHDTGLVTLISQNLNEFELHLRAVLGIPIPEIVCHEASASRVIL
ASMETTDVAFTGLEQALSQSNTNVFMFGKPSSTEGRRMGVAVAKAETIDEARIKADNAAQSIQFINE
>Mature_387_residues
MNVFPKKIMLLGSGELGKEVAIAAKRLGCYVIACDRYNDAPAMQIADQFNVFNMNNGSELKEVIYKCNPDIIIPEIEALA
VDVLKEIEQKITVIPNSRATATTMNRDKIRDLASNELNIRTAKFSYAVNQSELDLHAETIGYPLLIKPVMSSSGKGQSLV
KNKNELAQAWNLAIEKSRGKSNKIILEEFIDFDLEITLLTIRQSNGKTLFCAPIGHEQKNGDYQCSWQPAELTESVLEKA
QQIAKRVTDNLGGVGLFGVEFFIKGEEVIFSELSPRPHDTGLVTLISQNLNEFELHLRAVLGIPIPEIVCHEASASRVIL
ASMETTDVAFTGLEQALSQSNTNVFMFGKPSSTEGRRMGVAVAKAETIDEARIKADNAAQSIQFINE

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=378, Percent_Identity=48.6772486772487, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1786733, Length=334, Percent_Identity=23.3532934131737, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=369, Percent_Identity=26.0162601626016, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_PROM1 (A2C2S8)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015053.1
- ProteinModelPortal:   A2C2S8
- SMR:   A2C2S8
- STRING:   A2C2S8
- GeneID:   4781016
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_12301
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- BioCyc:   PMAR167555:NATL1_12301-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42664; Mature: 42664

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 75-75 BINDING 106-106 BINDING 147-147 BINDING 195-195 BINDING 260-260 BINDING 272-272 BINDING 279-279 BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFPKKIMLLGSGELGKEVAIAAKRLGCYVIACDRYNDAPAMQIADQFNVFNMNNGSEL
CCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCEEECCCCHHH
KEVIYKCNPDIIIPEIEALAVDVLKEIEQKITVIPNSRATATTMNRDKIRDLASNELNIR
HHHHHHCCCCEECCCHHHHHHHHHHHHHHHEEECCCCCCEEEECCHHHHHHHHCCCCEEE
TAKFSYAVNQSELDLHAETIGYPLLIKPVMSSSGKGQSLVKNKNELAQAWNLAIEKSRGK
EEEEEEEECCCCCEEEHHHCCCCEEEEHHHCCCCCCHHHHHCHHHHHHHHHHHEECCCCC
SNKIILEEFIDFDLEITLLTIRQSNGKTLFCAPIGHEQKNGDYQCSWQPAELTESVLEKA
CCCEEHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHH
QQIAKRVTDNLGGVGLFGVEFFIKGEEVIFSELSPRPHDTGLVTLISQNLNEFELHLRAV
HHHHHHHHHCCCCEEEEEEEEEEECCHHEEECCCCCCCCCCEEEEECCCCCHHHHHHHHH
LGIPIPEIVCHEASASRVILASMETTDVAFTGLEQALSQSNTNVFMFGKPSSTEGRRMGV
HCCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
AVAKAETIDEARIKADNAAQSIQFINE
EEEHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MNVFPKKIMLLGSGELGKEVAIAAKRLGCYVIACDRYNDAPAMQIADQFNVFNMNNGSEL
CCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCEEECCCCHHH
KEVIYKCNPDIIIPEIEALAVDVLKEIEQKITVIPNSRATATTMNRDKIRDLASNELNIR
HHHHHHCCCCEECCCHHHHHHHHHHHHHHHEEECCCCCCEEEECCHHHHHHHHCCCCEEE
TAKFSYAVNQSELDLHAETIGYPLLIKPVMSSSGKGQSLVKNKNELAQAWNLAIEKSRGK
EEEEEEEECCCCCEEEHHHCCCCEEEEHHHCCCCCCHHHHHCHHHHHHHHHHHEECCCCC
SNKIILEEFIDFDLEITLLTIRQSNGKTLFCAPIGHEQKNGDYQCSWQPAELTESVLEKA
CCCEEHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHH
QQIAKRVTDNLGGVGLFGVEFFIKGEEVIFSELSPRPHDTGLVTLISQNLNEFELHLRAV
HHHHHHHHHCCCCEEEEEEEEEEECCHHEEECCCCCCCCCCEEEEECCCCCHHHHHHHHH
LGIPIPEIVCHEASASRVILASMETTDVAFTGLEQALSQSNTNVFMFGKPSSTEGRRMGV
HCCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
AVAKAETIDEARIKADNAAQSIQFINE
EEEHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA