Click here to switch to the map view.

The map label for this gene is hisZ

Identifier: 124025687

GI number: 124025687

Start: 898347

End: 899522

Strand: Reverse

Name: hisZ

Synonym: NATL1_09801

Alternate gene names: 124025687

Gene position: 899522-898347 (Counterclockwise)

Preceding gene: 124025688

Following gene: 124025686

Centisome position: 48.24

GC content: 32.82

Gene sequence:

>1176_bases
ATGACATTGCAACCTGCCTCGGGTGCGCGCGACTTAAATCCTCAGCAAGTAAGAAAGAACCATCTCATTGCATCAAAACT
TTCCTCTCTATATCAACTATGGGGATATGAGCGAATATCACCGCCGCACATAGAACGTCTAGACACACTGACGGCAGCTG
GTGGGATTTCGAACAATGAAATACTAAAAATCGTTTCTGATGAACCTCTTGGATTAAGACCTGAAATAACAGCGTCAATT
GTTCGAGCTGCTTCAACTAGATTTAATGAATATGAAAGGCCACTTCGTTTTTGGTCAGCAGGAACCTCATTCAAATGCAA
TCAAAGCATTGATGGTGGTATTGATATTGAAGAGTCATTTCAAAGCGGAGTTGAATTGATAGGAACAAAAGCAATTAATG
CAGAAATAGAACTTCTATCTCTTCTCATCGAATCATTAGAAGTAATTGAAATTGATCAAAAATATAAAATGACATTGCTT
ATTGGAAATACATATCTATTGGAACTTATTTTAAGCTCATTTGATTCAACCAAGATAGACCAAATAAAAAAAATTCTCTG
CGATCTTGATTACATAGCATTGACTACACTTGATGTAAAAGAAGAACAAAGAATGTTTATCAAAACCATCATGAATATGA
GAGGAAAACCTGAAGAAGTGTTAACTAATCTACAAAACATATATGGCTCAAATTCATACATCGATAAACTAAAGGAATTA
TTTACCATTATTGAGCCTTTAGCTAAAGAGAAAGGAATAGAAGTACAACTAGACCCTACATTAGGAACCAAATATAAATT
ATATAGTGGATTAACATTTTCTCTTGTTTCATCATCAACAAGTGCTCCTGTTACTATTGCTAAAGGTGGTAGATATGATG
ATTTAGTTAAAAAATTTAGTTCAAGCGCCCAAAATTGCTATGGAATTGGTTTTAGTATTAGCGTTGATAAAGTAAGAGAA
TTAGTCTCCACAAGCAAAGAAAAACTTGTTAATAATGTAAAAGTTTTAATCGCATATAAGCAAAGTGCAAATTTGTATAA
AGCATTAAAACAACAAAAGGAATTGCATAGAAAGGGGATTATTTCAGTAATTTCACATGAACCTTTAAAGACAAATGATG
AAACAAATCAACTCTTAAAATCTAACAGATGTAATAAAATAGAATGGATAGATTGA

Upstream 100 bases:

>100_bases
CATAAAAGAACTTGGAAAAATTAAACCATTAACCCCAAACTCTTATGGTGGAAAATAGTCTTAATTTATGTGATCCTTAA
GAAATAAAAAGATTCAAATA

Downstream 100 bases:

>100_bases
TATAAATTAGCCTTAAATTTTTAATAATTGTCAGGGATTGAAAACTATTGAGCTGTAATCAATAACACTTTTGCATTTAA
CGCCTAAATAGAAATTTGTA

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTLQPASGARDLNPQQVRKNHLIASKLSSLYQLWGYERISPPHIERLDTLTAAGGISNNEILKIVSDEPLGLRPEITASI
VRAASTRFNEYERPLRFWSAGTSFKCNQSIDGGIDIEESFQSGVELIGTKAINAEIELLSLLIESLEVIEIDQKYKMTLL
IGNTYLLELILSSFDSTKIDQIKKILCDLDYIALTTLDVKEEQRMFIKTIMNMRGKPEEVLTNLQNIYGSNSYIDKLKEL
FTIIEPLAKEKGIEVQLDPTLGTKYKLYSGLTFSLVSSSTSAPVTIAKGGRYDDLVKKFSSSAQNCYGIGFSISVDKVRE
LVSTSKEKLVNNVKVLIAYKQSANLYKALKQQKELHRKGIISVISHEPLKTNDETNQLLKSNRCNKIEWID

Sequences:

>Translated_391_residues
MTLQPASGARDLNPQQVRKNHLIASKLSSLYQLWGYERISPPHIERLDTLTAAGGISNNEILKIVSDEPLGLRPEITASI
VRAASTRFNEYERPLRFWSAGTSFKCNQSIDGGIDIEESFQSGVELIGTKAINAEIELLSLLIESLEVIEIDQKYKMTLL
IGNTYLLELILSSFDSTKIDQIKKILCDLDYIALTTLDVKEEQRMFIKTIMNMRGKPEEVLTNLQNIYGSNSYIDKLKEL
FTIIEPLAKEKGIEVQLDPTLGTKYKLYSGLTFSLVSSSTSAPVTIAKGGRYDDLVKKFSSSAQNCYGIGFSISVDKVRE
LVSTSKEKLVNNVKVLIAYKQSANLYKALKQQKELHRKGIISVISHEPLKTNDETNQLLKSNRCNKIEWID
>Mature_390_residues
TLQPASGARDLNPQQVRKNHLIASKLSSLYQLWGYERISPPHIERLDTLTAAGGISNNEILKIVSDEPLGLRPEITASIV
RAASTRFNEYERPLRFWSAGTSFKCNQSIDGGIDIEESFQSGVELIGTKAINAEIELLSLLIESLEVIEIDQKYKMTLLI
GNTYLLELILSSFDSTKIDQIKKILCDLDYIALTTLDVKEEQRMFIKTIMNMRGKPEEVLTNLQNIYGSNSYIDKLKELF
TIIEPLAKEKGIEVQLDPTLGTKYKLYSGLTFSLVSSSTSAPVTIAKGGRYDDLVKKFSSSAQNCYGIGFSISVDKVREL
VSTSKEKLVNNVKVLIAYKQSANLYKALKQQKELHRKGIISVISHEPLKTNDETNQLLKSNRCNKIEWID

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_PROM1 (A2C228)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014803.1
- ProteinModelPortal:   A2C228
- STRING:   A2C228
- GeneID:   4779298
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_09801
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   PEIEVIN
- ProtClustDB:   PRK12292
- BioCyc:   PMAR167555:NATL1_09801-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 43906; Mature: 43775

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQPASGARDLNPQQVRKNHLIASKLSSLYQLWGYERISPPHIERLDTLTAAGGISNNE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
ILKIVSDEPLGLRPEITASIVRAASTRFNEYERPLRFWSAGTSFKCNQSIDGGIDIEESF
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHH
QSGVELIGTKAINAEIELLSLLIESLEVIEIDQKYKMTLLIGNTYLLELILSSFDSTKID
HCCHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHHHCCCCHHH
QIKKILCDLDYIALTTLDVKEEQRMFIKTIMNMRGKPEEVLTNLQNIYGSNSYIDKLKEL
HHHHHHHCCHHHHEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
FTIIEPLAKEKGIEVQLDPTLGTKYKLYSGLTFSLVSSSTSAPVTIAKGGRYDDLVKKFS
HHHHHHHHHCCCCEEEECCCCCCCEEHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHH
SSAQNCYGIGFSISVDKVRELVSTSKEKLVNNVKVLIAYKQSANLYKALKQQKELHRKGI
HCCCCEEECCEEEEHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
ISVISHEPLKTNDETNQLLKSNRCNKIEWID
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TLQPASGARDLNPQQVRKNHLIASKLSSLYQLWGYERISPPHIERLDTLTAAGGISNNE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
ILKIVSDEPLGLRPEITASIVRAASTRFNEYERPLRFWSAGTSFKCNQSIDGGIDIEESF
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHH
QSGVELIGTKAINAEIELLSLLIESLEVIEIDQKYKMTLLIGNTYLLELILSSFDSTKID
HCCHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHHHCCCCHHH
QIKKILCDLDYIALTTLDVKEEQRMFIKTIMNMRGKPEEVLTNLQNIYGSNSYIDKLKEL
HHHHHHHCCHHHHEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
FTIIEPLAKEKGIEVQLDPTLGTKYKLYSGLTFSLVSSSTSAPVTIAKGGRYDDLVKKFS
HHHHHHHHHCCCCEEEECCCCCCCEEHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHH
SSAQNCYGIGFSISVDKVRELVSTSKEKLVNNVKVLIAYKQSANLYKALKQQKELHRKGI
HCCCCEEECCEEEEHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
ISVISHEPLKTNDETNQLLKSNRCNKIEWID
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA