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The map label for this gene is ribE [H]

Identifier: 124025207

GI number: 124025207

Start: 452199

End: 452852

Strand: Direct

Name: ribE [H]

Synonym: NATL1_04961

Alternate gene names: 124025207

Gene position: 452199-452852 (Clockwise)

Preceding gene: 124025206

Following gene: 124025212

Centisome position: 24.25

GC content: 35.32

Gene sequence:

>654_bases
ATGTTTACTGGTTTAATTCAGGCTATTGGCACGATCAAGAAAAATAATTTGGGGGTAATTGTTGATGGATGTAACCCTTT
CTCTCCGTTAAAACTTGGAGATAGTGTGTCTGTTGATGGAGTGTGTCTAACAGTTTCTGAGTTGATGAATGATTCTTTTT
TCTCAAATATAAGTGAAGAGACTCTTAAGAGAACAAATCTTGCAGAAAAAGCTCAGAAAAATGGTTATGTAAATCTTGAG
CCAGCATTACGTCTGTCTGACCGATTAGGTGGACATATTGTAAGTGGACACATTGACGGGTTGGGTAAAGTTGTTTCAAT
CGAAAATTTGAAAAATTCTTGGAATTTGAGAGTATCTTGGGATGATTTAAACTTTTGCAGATATATGTGCGATAAGGCTA
GTATTAGCCTAAATGGAATAAGTCTTACTATTGCTGAGATCTATGTTGATGGGTGTGAATTTTCAGTAGCCGTAATACCT
CATACGTGGTCAAATACATGCTTGAAGTTTTTGACAATTGGAGAAAAGGTTAATTTGGAAGTTGACTTGATGGCTAAGTA
CGCAGAAAAACTTCTAAAAGTAAATAATAATGATTCCATTTCGAAACAAAGCCCAGTAATTAATTCTCAATGGCTAAGTG
AGCAAGGTTGGTAA

Upstream 100 bases:

>100_bases
TTGGAATAATTCATAACCGAACTATTTTTGAAATTCAAAAACCTTTTCAGAGACATTTCCAAATACCATAACTAGCATTC
TAAAAGTTCTAAGCTCAATA

Downstream 100 bases:

>100_bases
TAGTAATTTTATGTTTAATTTATATAATTTACTTTGAGAAATTTGTTATTTCTTGAGTGGTAACAAACTTATCGCTCCTG
ACTCTTTTCGTACATCAATA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MFTGLIQAIGTIKKNNLGVIVDGCNPFSPLKLGDSVSVDGVCLTVSELMNDSFFSNISEETLKRTNLAEKAQKNGYVNLE
PALRLSDRLGGHIVSGHIDGLGKVVSIENLKNSWNLRVSWDDLNFCRYMCDKASISLNGISLTIAEIYVDGCEFSVAVIP
HTWSNTCLKFLTIGEKVNLEVDLMAKYAEKLLKVNNNDSISKQSPVINSQWLSEQGW

Sequences:

>Translated_217_residues
MFTGLIQAIGTIKKNNLGVIVDGCNPFSPLKLGDSVSVDGVCLTVSELMNDSFFSNISEETLKRTNLAEKAQKNGYVNLE
PALRLSDRLGGHIVSGHIDGLGKVVSIENLKNSWNLRVSWDDLNFCRYMCDKASISLNGISLTIAEIYVDGCEFSVAVIP
HTWSNTCLKFLTIGEKVNLEVDLMAKYAEKLLKVNNNDSISKQSPVINSQWLSEQGW
>Mature_217_residues
MFTGLIQAIGTIKKNNLGVIVDGCNPFSPLKLGDSVSVDGVCLTVSELMNDSFFSNISEETLKRTNLAEKAQKNGYVNLE
PALRLSDRLGGHIVSGHIDGLGKVVSIENLKNSWNLRVSWDDLNFCRYMCDKASISLNGISLTIAEIYVDGCEFSVAVIP
HTWSNTCLKFLTIGEKVNLEVDLMAKYAEKLLKVNNNDSISKQSPVINSQWLSEQGW

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=188, Percent_Identity=33.5106382978723, Blast_Score=94, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319733, Length=223, Percent_Identity=37.6681614349776, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23918; Mature: 23918

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGLIQAIGTIKKNNLGVIVDGCNPFSPLKLGDSVSVDGVCLTVSELMNDSFFSNISEE
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHCHHHHHHHHHH
TLKRTNLAEKAQKNGYVNLEPALRLSDRLGGHIVSGHIDGLGKVVSIENLKNSWNLRVSW
HHHHHHHHHHHHHCCEEEECHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCEEEEEE
DDLNFCRYMCDKASISLNGISLTIAEIYVDGCEFSVAVIPHTWSNTCLKFLTIGEKVNLE
CCHHHHHHHHCCCCEEECCEEEEEEEEEECCCEEEEEEECCCCHHHHEEHEECCCCCCEE
VDLMAKYAEKLLKVNNNDSISKQSPVINSQWLSEQGW
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MFTGLIQAIGTIKKNNLGVIVDGCNPFSPLKLGDSVSVDGVCLTVSELMNDSFFSNISEE
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHCHHHHHHHHHH
TLKRTNLAEKAQKNGYVNLEPALRLSDRLGGHIVSGHIDGLGKVVSIENLKNSWNLRVSW
HHHHHHHHHHHHHCCEEEECHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCEEEEEE
DDLNFCRYMCDKASISLNGISLTIAEIYVDGCEFSVAVIPHTWSNTCLKFLTIGEKVNLE
CCHHHHHHHHCCCCEEECCEEEEEEEEEECCCEEEEEEECCCCHHHHEEHEECCCCCCEE
VDLMAKYAEKLLKVNNNDSISKQSPVINSQWLSEQGW
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]