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The map label for this gene is queA

Identifier: 124025170

GI number: 124025170

Start: 418643

End: 419740

Strand: Direct

Name: queA

Synonym: NATL1_04571

Alternate gene names: 124025170

Gene position: 418643-419740 (Clockwise)

Preceding gene: 124025169

Following gene: 124025175

Centisome position: 22.45

GC content: 38.34

Gene sequence:

>1098_bases
GTGTTAGATCAAAAAGATAATCTTTTAAGCTCATATAACTATGATTTACCTAAAGATTTAATTGCTCAATCACCTACCAG
GAGTAGGCATGATGCCAAATTGATGATTATAAAAGATGGCCTCGATGATTCTTTAAACCTTGTGCATGCAAAGGTATGGG
ACATAAAGGATATCTTGAAGCCTGCCGATCTTTTAGTTGTTAATAATACGCGAGTTGTTAAAGCAAGATTGAAAATTCGA
TTGTCAGGTGGAGGGGCAGGTGAATTATTGATAATGGAACCTAGGGGTGATGGCCGATGGCTTTGTCTTGGTCGTCCTGC
TAGGCGTATGAGAGGAGGTGATCAATTGTGGTTGGATACGTCACGAGATAATTCTTTAATCTTGAAAGTTATTGATAAGG
ATGAGAAAACAGGCGGAAGAATTATTCAATTTTCAAATGAGTTTACTAGTTGGACAGAAATGGAAAATTTACTTGATTTA
TGTGGAGAAGTCCCATTGCCTCCATATATAGATAAGGACAGATCTAGTGGTCATGAGGAAAACTATCAGACTAGATTTGC
TTCGAAACCAGGAGCCATAGCTGCTCCCACAGCGGGCTTACATCTAAGTGATGAACTTATAGAAAATTTAAAAATCAGAG
GCATTAGAATTGCGCAAATTACTTTGCATGTTGGCTTAGGAACCTTTAGACCACTAGAAAAAGAAGACCTATCTCAGCTG
CATTTACATAGTGAGTGGATAGAGGTTACTGAAGAGACCATAAAAGCGATAACTACTTGTAAGGAAGAGGGGGGGAAAGT
CTTTGCTGTAGGTACTACAAGTGTGAGAGCCCTTGAGGCTGCTTACCTTTCAGGAAGAGGCGCCTTGAAGCCGTATGAAG
GCAAAGTCAATTTGGTAATTAAACCAGGATTTAAATTTTCCGTCATTGATGGATTGCTAACTAACTTCCACCTACCTAAA
AGTTCCCTATTACTTTTAGTTAGCGCTCTAATTGGTCGAAATCGTATGTTGAAACTTTATAAACATGCTATCTCTAACAA
ATATCGATTTTTCTCTTATGGAGATGCGATGTTAATAACACCTGAGTCGGTTATTTAA

Upstream 100 bases:

>100_bases
GATTTGGCATACTTAATTGAAAAGAACCCTTATAGCCTCTCGTCTTGAGATTTAATTTGTTGAGCCACAAGTGATTTTTT
TTACTTTAATCTGATTTGAT

Downstream 100 bases:

>100_bases
ACTCAAAAGTCAAGCTAATGATGGTTCTTTAATAACGCCTGTAGGAACATTCTCAAACATTACGGATGAGATATATCTTT
CTGCAAGGTCAGGAAGAACC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MLDQKDNLLSSYNYDLPKDLIAQSPTRSRHDAKLMIIKDGLDDSLNLVHAKVWDIKDILKPADLLVVNNTRVVKARLKIR
LSGGGAGELLIMEPRGDGRWLCLGRPARRMRGGDQLWLDTSRDNSLILKVIDKDEKTGGRIIQFSNEFTSWTEMENLLDL
CGEVPLPPYIDKDRSSGHEENYQTRFASKPGAIAAPTAGLHLSDELIENLKIRGIRIAQITLHVGLGTFRPLEKEDLSQL
HLHSEWIEVTEETIKAITTCKEEGGKVFAVGTTSVRALEAAYLSGRGALKPYEGKVNLVIKPGFKFSVIDGLLTNFHLPK
SSLLLLVSALIGRNRMLKLYKHAISNKYRFFSYGDAMLITPESVI

Sequences:

>Translated_365_residues
MLDQKDNLLSSYNYDLPKDLIAQSPTRSRHDAKLMIIKDGLDDSLNLVHAKVWDIKDILKPADLLVVNNTRVVKARLKIR
LSGGGAGELLIMEPRGDGRWLCLGRPARRMRGGDQLWLDTSRDNSLILKVIDKDEKTGGRIIQFSNEFTSWTEMENLLDL
CGEVPLPPYIDKDRSSGHEENYQTRFASKPGAIAAPTAGLHLSDELIENLKIRGIRIAQITLHVGLGTFRPLEKEDLSQL
HLHSEWIEVTEETIKAITTCKEEGGKVFAVGTTSVRALEAAYLSGRGALKPYEGKVNLVIKPGFKFSVIDGLLTNFHLPK
SSLLLLVSALIGRNRMLKLYKHAISNKYRFFSYGDAMLITPESVI
>Mature_365_residues
MLDQKDNLLSSYNYDLPKDLIAQSPTRSRHDAKLMIIKDGLDDSLNLVHAKVWDIKDILKPADLLVVNNTRVVKARLKIR
LSGGGAGELLIMEPRGDGRWLCLGRPARRMRGGDQLWLDTSRDNSLILKVIDKDEKTGGRIIQFSNEFTSWTEMENLLDL
CGEVPLPPYIDKDRSSGHEENYQTRFASKPGAIAAPTAGLHLSDELIENLKIRGIRIAQITLHVGLGTFRPLEKEDLSQL
HLHSEWIEVTEETIKAITTCKEEGGKVFAVGTTSVRALEAAYLSGRGALKPYEGKVNLVIKPGFKFSVIDGLLTNFHLPK
SSLLLLVSALIGRNRMLKLYKHAISNKYRFFSYGDAMLITPESVI

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=360, Percent_Identity=40, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PROM1 (A2C0L1)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014286.1
- ProteinModelPortal:   A2C0L1
- SMR:   A2C0L1
- STRING:   A2C0L1
- GeneID:   4779155
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_04571
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   PMAR167555:NATL1_04571-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40785; Mature: 40785

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQKDNLLSSYNYDLPKDLIAQSPTRSRHDAKLMIIKDGLDDSLNLVHAKVWDIKDILK
CCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHCHHHHCC
PADLLVVNNTRVVKARLKIRLSGGGAGELLIMEPRGDGRWLCLGRPARRMRGGDQLWLDT
CCCEEEECCCEEEEEEEEEEECCCCCCEEEEEECCCCCCEEEECCCHHHCCCCCEEEEEC
SRDNSLILKVIDKDEKTGGRIIQFSNEFTSWTEMENLLDLCGEVPLPPYIDKDRSSGHEE
CCCCEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
NYQTRFASKPGAIAAPTAGLHLSDELIENLKIRGIRIAQITLHVGLGTFRPLEKEDLSQL
CHHHHHCCCCCCEECCCCCCEECHHHHHCCEECEEEEEEEEEEECCCCCCCCCHHHHHHH
HLHSEWIEVTEETIKAITTCKEEGGKVFAVGTTSVRALEAAYLSGRGALKPYEGKVNLVI
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEEE
KPGFKFSVIDGLLTNFHLPKSSLLLLVSALIGRNRMLKLYKHAISNKYRFFSYGDAMLIT
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCEEEEC
PESVI
CCCCC
>Mature Secondary Structure
MLDQKDNLLSSYNYDLPKDLIAQSPTRSRHDAKLMIIKDGLDDSLNLVHAKVWDIKDILK
CCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEEHHCHHHHCC
PADLLVVNNTRVVKARLKIRLSGGGAGELLIMEPRGDGRWLCLGRPARRMRGGDQLWLDT
CCCEEEECCCEEEEEEEEEEECCCCCCEEEEEECCCCCCEEEECCCHHHCCCCCEEEEEC
SRDNSLILKVIDKDEKTGGRIIQFSNEFTSWTEMENLLDLCGEVPLPPYIDKDRSSGHEE
CCCCEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
NYQTRFASKPGAIAAPTAGLHLSDELIENLKIRGIRIAQITLHVGLGTFRPLEKEDLSQL
CHHHHHCCCCCCEECCCCCCEECHHHHHCCEECEEEEEEEEEEECCCCCCCCCHHHHHHH
HLHSEWIEVTEETIKAITTCKEEGGKVFAVGTTSVRALEAAYLSGRGALKPYEGKVNLVI
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEEEE
KPGFKFSVIDGLLTNFHLPKSSLLLLVSALIGRNRMLKLYKHAISNKYRFFSYGDAMLIT
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCEEEEC
PESVI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA