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The map label for this gene is pdhC [H]

Identifier: 124025169

GI number: 124025169

Start: 417220

End: 418590

Strand: Direct

Name: pdhC [H]

Synonym: NATL1_04561

Alternate gene names: 124025169

Gene position: 417220-418590 (Clockwise)

Preceding gene: 124025168

Following gene: 124025170

Centisome position: 22.37

GC content: 42.67

Gene sequence:

>1371_bases
ATGGCCACTCATGACATTTTTATGCCTGCTTTAAGTTCAACGATGACTGAGGGCAAAATAGTTGAGTGGCTAAAAAAACC
TGGAGATAAAGTTGAAAGAGGTGAGTCAGTTTTAGTTGTTGAGTCAGATAAAGCTGATATGGATGTTGAGTCTTTCCAAG
ATGGCTTTCTTGCTTCGATTGTGATGCCTGCAGGCAGCTCTGCACCTGTTGGAGAGACAATCGGATTGATCGTTGAGACA
GAAGATGAGATCGCTGCGGCTCAAGCAAATTCGCCTTCTCCTTCCCCTCAATCAGGAAGTCAAGAAAAAGATAGTTCATC
GCCTCAAGTTCAAGAAAAACAAGCCTCTGTCGACTCTCCTAAAGCAACAGTAGTTACAAAGGCATCTCCTGCACCTCTTG
TTTCAGAATCCTCTGTAAATCAGGATCAGTTTTTAAATGATGGTCGAATAGTCGCTTCCCCAAGAGCTAAAAAACTTGCT
TCTCAAATGGGAGTGGATTTGGCAACTGTTAGGGGCTCAGGACCTCATGGACGAATACAAGCTGAGGATGTTCAAAGTGC
AAAAGGGCAACCCATAAGCGTTCCTTGGATTGCAGAAAGTAATGCTCCGGCGAAAATAGTTTCTGATGTGCCTCGCGTAG
AAAAAAAATCTGTTGACGCTGGTAAGCCACCTGCTCCAGGGAAAAGTTTTGGATCTAGAGGGGAAACAATTGCATTTAAT
ACTCTTCAACAAGCTGTAAATCGGAACATGGAGGAAAGTTTAAATACTCCTTGTTTCAGAGTCGGATATTCAATTCTTAC
TGATGAATTGGATGATCTTTATAAACAAGTTAAACCTGATGGAGTAACTATGACTGCTTTACTTGCTAAAGCAGTTGGCT
TAACGCTGGCTAGACACCCCCAGGTGAATGCAGCTTTTAGTTCTGAGGGGATTGCCTATCCTTCACAAATAAATGTAGCC
GTTGCAGTTGCGATGGAGGACGGAGGGTTGATAACTCCAGTGCTGCAAAATGCTGATAAGACGAGCCTTACTGATTTATC
CCTACAATGGGCTGATCTTGTTAAGCGAGCTAGGAATAAGCAATTAGAACCGCAAGAATATAGCAGTGGAACGTTTACAC
TCTCGAATCTAGGTATGTTTGGAGTGGATCGTTTTGATGCAATTCTGCCCCCAGGGACTGGAGCAATTTTAGCGGTAGGA
GCTTCATTGTCTAAAGTTGTTGCTTCTAAAGATGGTTCGATTTCAATCAAAAAACAAATGCAAGTAAATCTTACCGCTGA
TCACAGAGTGATATATGGGGCTGATGGAGCACTATTCCTCAAGGATTTGGCATACTTAATTGAAAAGAACCCTTATAGCC
TCTCGTCTTGA

Upstream 100 bases:

>100_bases
GTAGAACTAACATTGGTATGTATTGATACTTGCAAAGCTTCTAGATAGAAAATTTTGAAGCCTTGCCACAAACTCTCCAT
AAAGGAAATAGGAACAAATT

Downstream 100 bases:

>100_bases
GATTTAATTTGTTGAGCCACAAGTGATTTTTTTTACTTTAATCTGATTTGATGTGTTAGATCAAAAAGATAATCTTTTAA
GCTCATATAACTATGATTTA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MATHDIFMPALSSTMTEGKIVEWLKKPGDKVERGESVLVVESDKADMDVESFQDGFLASIVMPAGSSAPVGETIGLIVET
EDEIAAAQANSPSPSPQSGSQEKDSSSPQVQEKQASVDSPKATVVTKASPAPLVSESSVNQDQFLNDGRIVASPRAKKLA
SQMGVDLATVRGSGPHGRIQAEDVQSAKGQPISVPWIAESNAPAKIVSDVPRVEKKSVDAGKPPAPGKSFGSRGETIAFN
TLQQAVNRNMEESLNTPCFRVGYSILTDELDDLYKQVKPDGVTMTALLAKAVGLTLARHPQVNAAFSSEGIAYPSQINVA
VAVAMEDGGLITPVLQNADKTSLTDLSLQWADLVKRARNKQLEPQEYSSGTFTLSNLGMFGVDRFDAILPPGTGAILAVG
ASLSKVVASKDGSISIKKQMQVNLTADHRVIYGADGALFLKDLAYLIEKNPYSLSS

Sequences:

>Translated_456_residues
MATHDIFMPALSSTMTEGKIVEWLKKPGDKVERGESVLVVESDKADMDVESFQDGFLASIVMPAGSSAPVGETIGLIVET
EDEIAAAQANSPSPSPQSGSQEKDSSSPQVQEKQASVDSPKATVVTKASPAPLVSESSVNQDQFLNDGRIVASPRAKKLA
SQMGVDLATVRGSGPHGRIQAEDVQSAKGQPISVPWIAESNAPAKIVSDVPRVEKKSVDAGKPPAPGKSFGSRGETIAFN
TLQQAVNRNMEESLNTPCFRVGYSILTDELDDLYKQVKPDGVTMTALLAKAVGLTLARHPQVNAAFSSEGIAYPSQINVA
VAVAMEDGGLITPVLQNADKTSLTDLSLQWADLVKRARNKQLEPQEYSSGTFTLSNLGMFGVDRFDAILPPGTGAILAVG
ASLSKVVASKDGSISIKKQMQVNLTADHRVIYGADGALFLKDLAYLIEKNPYSLSS
>Mature_455_residues
ATHDIFMPALSSTMTEGKIVEWLKKPGDKVERGESVLVVESDKADMDVESFQDGFLASIVMPAGSSAPVGETIGLIVETE
DEIAAAQANSPSPSPQSGSQEKDSSSPQVQEKQASVDSPKATVVTKASPAPLVSESSVNQDQFLNDGRIVASPRAKKLAS
QMGVDLATVRGSGPHGRIQAEDVQSAKGQPISVPWIAESNAPAKIVSDVPRVEKKSVDAGKPPAPGKSFGSRGETIAFNT
LQQAVNRNMEESLNTPCFRVGYSILTDELDDLYKQVKPDGVTMTALLAKAVGLTLARHPQVNAAFSSEGIAYPSQINVAV
AVAMEDGGLITPVLQNADKTSLTDLSLQWADLVKRARNKQLEPQEYSSGTFTLSNLGMFGVDRFDAILPPGTGAILAVGA
SLSKVVASKDGSISIKKQMQVNLTADHRVIYGADGALFLKDLAYLIEKNPYSLSS

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=457, Percent_Identity=29.7592997811816, Blast_Score=185, Evalue=9e-47,
Organism=Homo sapiens, GI203098816, Length=485, Percent_Identity=29.0721649484536, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI203098753, Length=485, Percent_Identity=29.0721649484536, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI110671329, Length=444, Percent_Identity=26.1261261261261, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI260898739, Length=174, Percent_Identity=33.9080459770115, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI19923748, Length=239, Percent_Identity=28.0334728033473, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786946, Length=456, Percent_Identity=25.219298245614, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786305, Length=310, Percent_Identity=30.6451612903226, Blast_Score=111, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560088, Length=468, Percent_Identity=31.4102564102564, Blast_Score=182, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=458, Percent_Identity=25.1091703056769, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17538894, Length=294, Percent_Identity=31.9727891156463, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25146366, Length=233, Percent_Identity=30.0429184549356, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324258, Length=185, Percent_Identity=40.5405405405405, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=262, Percent_Identity=32.0610687022901, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321632, Length=199, Percent_Identity=31.1557788944724, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI20129315, Length=452, Percent_Identity=29.4247787610619, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24582497, Length=445, Percent_Identity=28.9887640449438, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=27.3972602739726, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24645909, Length=244, Percent_Identity=29.9180327868852, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48224; Mature: 48093

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHDIFMPALSSTMTEGKIVEWLKKPGDKVERGESVLVVESDKADMDVESFQDGFLASI
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCEEEEEECCCCCCCHHHHHCCCEEEE
VMPAGSSAPVGETIGLIVETEDEIAAAQANSPSPSPQSGSQEKDSSSPQVQEKQASVDSP
EECCCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
KATVVTKASPAPLVSESSVNQDQFLNDGRIVASPRAKKLASQMGVDLATVRGSGPHGRIQ
CEEEEECCCCCCCCCCCCCCHHHHCCCCEEEECCHHHHHHHHHCCEEEEEECCCCCCCEE
AEDVQSAKGQPISVPWIAESNAPAKIVSDVPRVEKKSVDAGKPPAPGKSFGSRGETIAFN
HHHHHHCCCCCEECEEEECCCCCHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCEEEHH
TLQQAVNRNMEESLNTPCFRVGYSILTDELDDLYKQVKPDGVTMTALLAKAVGLTLARHP
HHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
QVNAAFSSEGIAYPSQINVAVAVAMEDGGLITPVLQNADKTSLTDLSLQWADLVKRARNK
CCCCEECCCCCCCCCCCCEEEEEEECCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCC
QLEPQEYSSGTFTLSNLGMFGVDRFDAILPPGTGAILAVGASLSKVVASKDGSISIKKQM
CCCCCCCCCCCEEEECCCCCCCCHHHHCCCCCCCEEEEECCHHHHHHHCCCCCEEEEEEE
QVNLTADHRVIYGADGALFLKDLAYLIEKNPYSLSS
EEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ATHDIFMPALSSTMTEGKIVEWLKKPGDKVERGESVLVVESDKADMDVESFQDGFLASI
CCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCEEEEEECCCCCCCHHHHHCCCEEEE
VMPAGSSAPVGETIGLIVETEDEIAAAQANSPSPSPQSGSQEKDSSSPQVQEKQASVDSP
EECCCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
KATVVTKASPAPLVSESSVNQDQFLNDGRIVASPRAKKLASQMGVDLATVRGSGPHGRIQ
CEEEEECCCCCCCCCCCCCCHHHHCCCCEEEECCHHHHHHHHHCCEEEEEECCCCCCCEE
AEDVQSAKGQPISVPWIAESNAPAKIVSDVPRVEKKSVDAGKPPAPGKSFGSRGETIAFN
HHHHHHCCCCCEECEEEECCCCCHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCEEEHH
TLQQAVNRNMEESLNTPCFRVGYSILTDELDDLYKQVKPDGVTMTALLAKAVGLTLARHP
HHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
QVNAAFSSEGIAYPSQINVAVAVAMEDGGLITPVLQNADKTSLTDLSLQWADLVKRARNK
CCCCEECCCCCCCCCCCCEEEEEEECCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCC
QLEPQEYSSGTFTLSNLGMFGVDRFDAILPPGTGAILAVGASLSKVVASKDGSISIKKQM
CCCCCCCCCCCEEEECCCCCCCCHHHHCCCCCCCEEEEECCHHHHHHHCCCCCEEEEEEE
QVNLTADHRVIYGADGALFLKDLAYLIEKNPYSLSS
EEEEEECCEEEEECCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA