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The map label for this gene is hemK [C]

Identifier: 124025122

GI number: 124025122

Start: 376120

End: 376941

Strand: Direct

Name: hemK [C]

Synonym: NATL1_04091

Alternate gene names: 124025122

Gene position: 376120-376941 (Clockwise)

Preceding gene: 124025121

Following gene: 124025123

Centisome position: 20.17

GC content: 35.64

Gene sequence:

>822_bases
ATGATATTGATAGGCGGTAGGAAAGTTGATTTTGATTGGCTTTTGGATATGGTCGCTGGAGTCTCTTGGGCTAAATTGCA
AAGCATTATTTTGAATCCTGAGAAGTTTATTTCCTTAGATATATCAACTGATGAATTAGAAGTTATTTGGAAATCTCATT
TAAAAGATCAAACCCCACTTCAGTATTTGATCTCTAAGTGCCCTTGGAGAGATGTTGAGTTAGAGGTTTCAGCAGAGGCT
CTTATCCCTAGGCAAGAAACAGAATTTTTGATTGATATTGCTTTGAAAAAAATTACTAATTTTGATTCTGGAAGATGGGC
TGATCTTGGTACAGGCTCTGGAGCTATTGCAGTATCTTTAGCTAAATCTCTTCCTAATTGGAATGGACACGCAGTTGATA
TTAGTAATGAAGCATTAGAACTAGCAAAAAGAAATTTAAAAGCTATTGTGCCAAATGCGAATGTGAGATTTTCTTTGGGT
GATTGGTGGGAACCTTTGAAAAGATGGAGGGGAAGATTTGATTTAGTTTTAAGTAATCCTCCTTATATACCCTCCGATTT
AGTTGAAGAGTTAGAACCAGTTGTTAAAAACCATGAGCCAATTATTGCTTTGGATGGTGGAGAGGATGGAATGAATGCTT
CTAGAAAAATCATCCTTGGAGCATTAAATGGACTTGCCAAAGGAGGTTGGTTGATTCTTGAACATCATTATGATCAAAGT
GAAAAGATAACTAGTTTTATGAGGAATATTGGAATGGAAGAAGTTTCATTTGAAAAAGATATAAGTGGAATCAAGCGCTA
TGCAATTTGTCGCAGAAAATGA

Upstream 100 bases:

>100_bases
ATTATTAATGCTAGAAGAGGGACCAAAATTTTAAGGAATTGAAGAATCTATTATGTTTAAATCCATTCCAGGTGAAGCTT
TTCTTAAATGGAGAAAAAAG

Downstream 100 bases:

>100_bases
AATGATCACCCAGCAATTTGGAATCTTTGACTTGGCTTTAAAGTTGAAAAAGGGATCTTTAGCTTTATTTCCTACTGATA
CTTTGCCAGCACTGTGTTCA

Product: putative protein methyltransferase

Products: Protoporphyrin IX; H2O

Alternate protein names: M.Ssp6803HemKP [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MILIGGRKVDFDWLLDMVAGVSWAKLQSIILNPEKFISLDISTDELEVIWKSHLKDQTPLQYLISKCPWRDVELEVSAEA
LIPRQETEFLIDIALKKITNFDSGRWADLGTGSGAIAVSLAKSLPNWNGHAVDISNEALELAKRNLKAIVPNANVRFSLG
DWWEPLKRWRGRFDLVLSNPPYIPSDLVEELEPVVKNHEPIIALDGGEDGMNASRKIILGALNGLAKGGWLILEHHYDQS
EKITSFMRNIGMEEVSFEKDISGIKRYAICRRK

Sequences:

>Translated_273_residues
MILIGGRKVDFDWLLDMVAGVSWAKLQSIILNPEKFISLDISTDELEVIWKSHLKDQTPLQYLISKCPWRDVELEVSAEA
LIPRQETEFLIDIALKKITNFDSGRWADLGTGSGAIAVSLAKSLPNWNGHAVDISNEALELAKRNLKAIVPNANVRFSLG
DWWEPLKRWRGRFDLVLSNPPYIPSDLVEELEPVVKNHEPIIALDGGEDGMNASRKIILGALNGLAKGGWLILEHHYDQS
EKITSFMRNIGMEEVSFEKDISGIKRYAICRRK
>Mature_273_residues
MILIGGRKVDFDWLLDMVAGVSWAKLQSIILNPEKFISLDISTDELEVIWKSHLKDQTPLQYLISKCPWRDVELEVSAEA
LIPRQETEFLIDIALKKITNFDSGRWADLGTGSGAIAVSLAKSLPNWNGHAVDISNEALELAKRNLKAIVPNANVRFSLG
DWWEPLKRWRGRFDLVLSNPPYIPSDLVEELEPVVKNHEPIIALDGGEDGMNASRKIILGALNGLAKGGWLILEHHYDQS
EKITSFMRNIGMEEVSFEKDISGIKRYAICRRK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=250, Percent_Identity=28, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787463, Length=221, Percent_Identity=32.579185520362, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87082085, Length=181, Percent_Identity=31.4917127071823, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582226, Length=231, Percent_Identity=30.3030303030303, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 1.3.3.4

Molecular weight: Translated: 30786; Mature: 30786

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIGGRKVDFDWLLDMVAGVSWAKLQSIILNPEKFISLDISTDELEVIWKSHLKDQTPL
CEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHEEEEECCCHHHHHHHHHHCCCCCHH
QYLISKCPWRDVELEVSAEALIPRQETEFLIDIALKKITNFDSGRWADLGTGSGAIAVSL
HHHHHHCCCCCEEEEECHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHH
AKSLPNWNGHAVDISNEALELAKRNLKAIVPNANVRFSLGDWWEPLKRWRGRFDLVLSNP
HHHCCCCCCCEEECCHHHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHCCCEEEEECCC
PYIPSDLVEELEPVVKNHEPIIALDGGEDGMNASRKIILGALNGLAKGGWLILEHHYDQS
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHEEHHHHHCCCCEEEEEECCCCH
EKITSFMRNIGMEEVSFEKDISGIKRYAICRRK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILIGGRKVDFDWLLDMVAGVSWAKLQSIILNPEKFISLDISTDELEVIWKSHLKDQTPL
CEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHEEEEECCCHHHHHHHHHHCCCCCHH
QYLISKCPWRDVELEVSAEALIPRQETEFLIDIALKKITNFDSGRWADLGTGSGAIAVSL
HHHHHHCCCCCEEEEECHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHH
AKSLPNWNGHAVDISNEALELAKRNLKAIVPNANVRFSLGDWWEPLKRWRGRFDLVLSNP
HHHCCCCCCCEEECCHHHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHCCCEEEEECCC
PYIPSDLVEELEPVVKNHEPIIALDGGEDGMNASRKIILGALNGLAKGGWLILEHHYDQS
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHEEHHHHHCCCCEEEEEECCCCH
EKITSFMRNIGMEEVSFEKDISGIKRYAICRRK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protoporphyrinogen IX; O2

Specific reaction: protoporphyrinogen-IX + 1.5 O2 = protoporphyrin-IX + 3 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]