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The map label for this gene is pyrE

Identifier: 124025068

GI number: 124025068

Start: 327703

End: 328284

Strand: Direct

Name: pyrE

Synonym: NATL1_03551

Alternate gene names: 124025068

Gene position: 327703-328284 (Clockwise)

Preceding gene: 124025064

Following gene: 124025069

Centisome position: 17.57

GC content: 36.94

Gene sequence:

>582_bases
ATGAATCCATTGAATCCTTCATCAGATGGAATAAAAGAAAATCTTTTAACTTTATTAGCTCAAAAAGCTTATAGGTTTGG
CGATTTTTCTTTGGCTTCCGGAAAAAAAAGTTCTCATTACGTCAATTGCAAGCCAGTCTCACTCTCAGGTCCAGGTCTAT
TGTCGATAAGTTCATTATTCCTAAAACAAATTAATGAAAGTGATAGTGGCGTAGCTGGCTTAACTTTAGGTGCTGACCCA
CTTGTTAGTGGCGTTGTGATTTTGGCAGCTCAATCAGGTATTGATTTAAACGGTTTAATAGTTAGGAAGGAAGCGAAAGG
TCATGGGACTGGAGCGTGGTTAGAAGGACCTTTACCTCCAAAGGGTTCTGTGATTACTGTCTTAGAGGATGTTGTTACGA
CTGGTGGTTCTTCTTTAAAAGCAGTTGAACAACTTAGAAATCAAGGGTATTTAGTTAAACAAGTACTAGCAATAGTTGAC
AGAGAAGAAGGAGGCCTAGATGCAATTTCTAAAGCTGATTTAGAGTTAAATAGTCTTTTCTTTTTAAAAGAGATTGTTGA
AAGAGCTAATAGTTTGCAATGA

Upstream 100 bases:

>100_bases
GAACTAAAACTTTTTTTGATAATAGTGAATTAGTTTGCCTAAAGTAAATATATATCTAATCCCACCAAGATGTTTAATAA
TTAAACTTGCATATAAAGAT

Downstream 100 bases:

>100_bases
CAGAATCCCAAGCATTGATTTGGGATGAAACATTCCCTTCATTACTTCTCAAAGGTCAGGGGACTACTTCTTTTTTACAT
GGTCAAACAACCGCTGACGT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MNPLNPSSDGIKENLLTLLAQKAYRFGDFSLASGKKSSHYVNCKPVSLSGPGLLSISSLFLKQINESDSGVAGLTLGADP
LVSGVVILAAQSGIDLNGLIVRKEAKGHGTGAWLEGPLPPKGSVITVLEDVVTTGGSSLKAVEQLRNQGYLVKQVLAIVD
REEGGLDAISKADLELNSLFFLKEIVERANSLQ

Sequences:

>Translated_193_residues
MNPLNPSSDGIKENLLTLLAQKAYRFGDFSLASGKKSSHYVNCKPVSLSGPGLLSISSLFLKQINESDSGVAGLTLGADP
LVSGVVILAAQSGIDLNGLIVRKEAKGHGTGAWLEGPLPPKGSVITVLEDVVTTGGSSLKAVEQLRNQGYLVKQVLAIVD
REEGGLDAISKADLELNSLFFLKEIVERANSLQ
>Mature_193_residues
MNPLNPSSDGIKENLLTLLAQKAYRFGDFSLASGKKSSHYVNCKPVSLSGPGLLSISSLFLKQINESDSGVAGLTLGADP
LVSGVVILAAQSGIDLNGLIVRKEAKGHGTGAWLEGPLPPKGSVITVLEDVVTTGGSSLKAVEQLRNQGYLVKQVLAIVD
REEGGLDAISKADLELNSLFFLKEIVERANSLQ

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=170, Percent_Identity=34.7058823529412, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17554964, Length=182, Percent_Identity=36.8131868131868, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508631, Length=179, Percent_Identity=30.1675977653631, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17933654, Length=180, Percent_Identity=34.4444444444444, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_PROM1 (A2C0A9)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014184.1
- ProteinModelPortal:   A2C0A9
- SMR:   A2C0A9
- STRING:   A2C0A9
- GeneID:   4780396
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_03551
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   PRK00455
- BioCyc:   PMAR167555:NATL1_03551-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 20284; Mature: 20284

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 102-102 BINDING 103-103 BINDING 106-106 BINDING 108-108 BINDING 133-133 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLNPSSDGIKENLLTLLAQKAYRFGDFSLASGKKSSHYVNCKPVSLSGPGLLSISSLF
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCCCCHHHHHHHH
LKQINESDSGVAGLTLGADPLVSGVVILAAQSGIDLNGLIVRKEAKGHGTGAWLEGPLPP
HHHHCCCCCCEEEEEECCCHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCEECCCCCC
KGSVITVLEDVVTTGGSSLKAVEQLRNQGYLVKQVLAIVDREEGGLDAISKADLELNSLF
CCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHH
FLKEIVERANSLQ
HHHHHHHHHHCCC
>Mature Secondary Structure
MNPLNPSSDGIKENLLTLLAQKAYRFGDFSLASGKKSSHYVNCKPVSLSGPGLLSISSLF
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCCCCHHHHHHHH
LKQINESDSGVAGLTLGADPLVSGVVILAAQSGIDLNGLIVRKEAKGHGTGAWLEGPLPP
HHHHCCCCCCEEEEEECCCHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCEECCCCCC
KGSVITVLEDVVTTGGSSLKAVEQLRNQGYLVKQVLAIVDREEGGLDAISKADLELNSLF
CCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHH
FLKEIVERANSLQ
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA