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The map label for this gene is trmB

Identifier: 124025032

GI number: 124025032

Start: 297616

End: 298260

Strand: Direct

Name: trmB

Synonym: NATL1_03191

Alternate gene names: 124025032

Gene position: 297616-298260 (Clockwise)

Preceding gene: 124025030

Following gene: 124025033

Centisome position: 15.96

GC content: 27.6

Gene sequence:

>645_bases
GTGAGACAGCATGTAAATCCTTTAAGTCAATTTTTTCAGTTACCATTATCTCTTCCTAGTAAGAATATTCTTTTTGAGAA
ATCTCACTATCCAATTCATTTAGATATTGGATCTGCAAAAGGTGAATTTCTGATTGAATTAGCAACAAAATACCCTGACT
GGAATTTTGTTGGGCTTGAGATAAGAGAACCTCTTGTTAGTTTATGTGAAAAGAAAAGAAGAAAATTAGAATTGACTAAT
TTAAAGTTTCTATTTTGTAATGTTAATGTCAGTCTAGATGAATGGTTATCTGACTTAGATTTTGGTCAATTAAAAAGAGT
TTCAATTCAATTCCCAGATCCTTGGTTTAAAAGAAAACACTTTAAAAGGAGAGTCCTAAATACTAATATTCTCAATTCAA
TTGCTAAATCTATGAGTAAAGATGGCGAAATATTTATTCAAAGCGATATACTCAAACTTATTGAATATATGACTAATACG
ATTGATGAAAATAGATATTTTACTAGAAAAAATGTAGGAGATCTGAGGTCAATTGATAAAAATCCTTATGATGTAATGAC
AGATAGAGAAATATTTTCTCTTAAGAAAAATTTGCTAATTTATAGAGTAATGTATATTAGAAATAGCCTATTATTCACTA
ATTAA

Upstream 100 bases:

>100_bases
TCAAAAATTTATAAATTGGTTTGTCTTCATATCATCTTCTGAAAAATTTAATTGACTTGCTAATATAATAATCTGATTTA
TTTATTTCAGGTTTCATTTT

Downstream 100 bases:

>100_bases
TCAAATTTATAATTACAATATATGATATGTTTTAATTTAAAGTTTATGTATATAATCATTAGTATGAAGTGTGAACTCGC
ATGATTGATAACTCCAATTC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRQHVNPLSQFFQLPLSLPSKNILFEKSHYPIHLDIGSAKGEFLIELATKYPDWNFVGLEIREPLVSLCEKKRRKLELTN
LKFLFCNVNVSLDEWLSDLDFGQLKRVSIQFPDPWFKRKHFKRRVLNTNILNSIAKSMSKDGEIFIQSDILKLIEYMTNT
IDENRYFTRKNVGDLRSIDKNPYDVMTDREIFSLKKNLLIYRVMYIRNSLLFTN

Sequences:

>Translated_214_residues
MRQHVNPLSQFFQLPLSLPSKNILFEKSHYPIHLDIGSAKGEFLIELATKYPDWNFVGLEIREPLVSLCEKKRRKLELTN
LKFLFCNVNVSLDEWLSDLDFGQLKRVSIQFPDPWFKRKHFKRRVLNTNILNSIAKSMSKDGEIFIQSDILKLIEYMTNT
IDENRYFTRKNVGDLRSIDKNPYDVMTDREIFSLKKNLLIYRVMYIRNSLLFTN
>Mature_214_residues
MRQHVNPLSQFFQLPLSLPSKNILFEKSHYPIHLDIGSAKGEFLIELATKYPDWNFVGLEIREPLVSLCEKKRRKLELTN
LKFLFCNVNVSLDEWLSDLDFGQLKRVSIQFPDPWFKRKHFKRRVLNTNILNSIAKSMSKDGEIFIQSDILKLIEYMTNT
IDENRYFTRKNVGDLRSIDKNPYDVMTDREIFSLKKNLLIYRVMYIRNSLLFTN

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Homo sapiens, GI167004078, Length=147, Percent_Identity=29.9319727891156, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789330, Length=144, Percent_Identity=26.3888888888889, Blast_Score=68, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320000, Length=140, Percent_Identity=29.2857142857143, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_PROM1 (A2C073)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014148.1
- ProteinModelPortal:   A2C073
- SMR:   A2C073
- STRING:   A2C073
- GeneID:   4780488
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_03191
- eggNOG:   COG0220
- HOGENOM:   HBG284653
- OMA:   PIHLDIG
- ProtClustDB:   PRK00121
- BioCyc:   PMAR167555:NATL1_03191-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25428; Mature: 25428

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: BINDING 35-35 BINDING 60-60 BINDING 87-87 BINDING 113-113 BINDING 117-117 BINDING 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHVNPLSQFFQLPLSLPSKNILFEKSHYPIHLDIGSAKGEFLIELATKYPDWNFVGLE
CCCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCEEEHH
IREPLVSLCEKKRRKLELTNLKFLFCNVNVSLDEWLSDLDFGQLKRVSIQFPDPWFKRKH
HHHHHHHHHHHHHHHEEECCEEEEEEEECCCHHHHHHHCCCCCEEEEEEECCCHHHHHHH
FKRRVLNTNILNSIAKSMSKDGEIFIQSDILKLIEYMTNTIDENRYFTRKNVGDLRSIDK
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCC
NPYDVMTDREIFSLKKNLLIYRVMYIRNSLLFTN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRQHVNPLSQFFQLPLSLPSKNILFEKSHYPIHLDIGSAKGEFLIELATKYPDWNFVGLE
CCCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCEEEHH
IREPLVSLCEKKRRKLELTNLKFLFCNVNVSLDEWLSDLDFGQLKRVSIQFPDPWFKRKH
HHHHHHHHHHHHHHHEEECCEEEEEEEECCCHHHHHHHCCCCCEEEEEEECCCHHHHHHH
FKRRVLNTNILNSIAKSMSKDGEIFIQSDILKLIEYMTNTIDENRYFTRKNVGDLRSIDK
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCC
NPYDVMTDREIFSLKKNLLIYRVMYIRNSLLFTN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA