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The map label for this gene is prfB [H]

Identifier: 124024969

GI number: 124024969

Start: 235672

End: 236613

Strand: Direct

Name: prfB [H]

Synonym: NATL1_02561

Alternate gene names: 124024969

Gene position: 235672-236613 (Clockwise)

Preceding gene: 124024968

Following gene: 124024970

Centisome position: 12.64

GC content: 39.7

Gene sequence:

>942_bases
ATGCGTCAACTTGATGAGGTTAAAGCACAGTTAGAAAAATTAATTACATGGAGGGGGGCTATTGAGGACGCAAATCTTTC
GTTAGAGCTTTATGAGTTAGATCTGGATGAAGAAATCCTGTCTGAGGCTCACAATGGGCTCGAGAAATTAAAACAAGAAC
TTGATCGTTGGGAGACGGAGCGCCTCTTAAGTGGCATCTATGATAAGGAGGGTGCAGTTCTATCCATTAATGCTGGTGCT
GGCGGAACTGATGCCCAAGATTGGGCTCAAATGCTACTAAGGATGTATACCCGTTGGGCTGACAATAATGGTATGAAAGT
GATCATTAATGAACTTTCTGAGGGAGAGGAAGCTGGAATAAAAAGTGTCACTATCGAAATTGAAGGTGTTTACGCATATG
GTTTTTTACAAAATGAAAAAGGAACTCATCGTTTAGTCAGGATTTCCCCTTTCAATGCAAATGGAAAGCGACAAACTAGT
TTCGCTGGAGTTGAAGTTATGCCTAAACTTGATGAAGAGGTTGAACTTGAGATCCCTGAAAAGGATTTAGAAATTACAAC
AAGTCGCTCAGGTGGTGCAGGTGGACAAAATGTAAATAAAGTTGAGACTGCGGTTCGAATTCTTCATGTGCCTACGGGTA
TTGCCGTTAGATGCACACAAGAAAGATCTCAGCTTCAAAATAAAGACAAGGCAATGGCACTATTAAAATCCAAATTGATG
GTCATAGCATTAGAGCAAAGGGCTGCAGAGATTGCTGATATTCGGGGGGATATTGTTGAAGCTGCGTGGGGCAATCAAAT
TAGAAATTATGTTTTTCACCCGTACCAAATGGTAAAAGATTTACGCACTGAACAAGAGACAACTGATGTAGATTCAGTAA
TGAATGGAGAAATAGATATTTTTATTCAGTCTCTTTTAAGGCAAAATATTGAAGAATCATAA

Upstream 100 bases:

>100_bases
TGTCTTTGACGAACCTGCATTACTTGCTAGACAAAGGGATTTAGAGCAAGTTGCATCGCAACCTGAGTTTTGGAATGATC
CTCAAAATGCGCAAAAACAG

Downstream 100 bases:

>100_bases
ATCAACTAGATATGAATAATAAAATTATTTCTGATGACGTCACCAAGAAAGCAACCCCACCTCCAAGTTTTGTCAAACTT
GCGATGAAAAATATGGTTCG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRQLDEVKAQLEKLITWRGAIEDANLSLELYELDLDEEILSEAHNGLEKLKQELDRWETERLLSGIYDKEGAVLSINAGA
GGTDAQDWAQMLLRMYTRWADNNGMKVIINELSEGEEAGIKSVTIEIEGVYAYGFLQNEKGTHRLVRISPFNANGKRQTS
FAGVEVMPKLDEEVELEIPEKDLEITTSRSGGAGGQNVNKVETAVRILHVPTGIAVRCTQERSQLQNKDKAMALLKSKLM
VIALEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTEQETTDVDSVMNGEIDIFIQSLLRQNIEES

Sequences:

>Translated_313_residues
MRQLDEVKAQLEKLITWRGAIEDANLSLELYELDLDEEILSEAHNGLEKLKQELDRWETERLLSGIYDKEGAVLSINAGA
GGTDAQDWAQMLLRMYTRWADNNGMKVIINELSEGEEAGIKSVTIEIEGVYAYGFLQNEKGTHRLVRISPFNANGKRQTS
FAGVEVMPKLDEEVELEIPEKDLEITTSRSGGAGGQNVNKVETAVRILHVPTGIAVRCTQERSQLQNKDKAMALLKSKLM
VIALEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTEQETTDVDSVMNGEIDIFIQSLLRQNIEES
>Mature_313_residues
MRQLDEVKAQLEKLITWRGAIEDANLSLELYELDLDEEILSEAHNGLEKLKQELDRWETERLLSGIYDKEGAVLSINAGA
GGTDAQDWAQMLLRMYTRWADNNGMKVIINELSEGEEAGIKSVTIEIEGVYAYGFLQNEKGTHRLVRISPFNANGKRQTS
FAGVEVMPKLDEEVELEIPEKDLEITTSRSGGAGGQNVNKVETAVRILHVPTGIAVRCTQERSQLQNKDKAMALLKSKLM
VIALEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTEQETTDVDSVMNGEIDIFIQSLLRQNIEES

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=241, Percent_Identity=34.8547717842324, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI34577120, Length=256, Percent_Identity=32.8125, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI166795305, Length=145, Percent_Identity=34.4827586206897, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI2367172, Length=304, Percent_Identity=45.7236842105263, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=37.037037037037, Blast_Score=158, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17542784, Length=242, Percent_Identity=33.0578512396694, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321295, Length=240, Percent_Identity=30.8333333333333, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921226, Length=211, Percent_Identity=37.914691943128, Blast_Score=131, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35220; Mature: 35220

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLDEVKAQLEKLITWRGAIEDANLSLELYELDLDEEILSEAHNGLEKLKQELDRWETE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RLLSGIYDKEGAVLSINAGAGGTDAQDWAQMLLRMYTRWADNNGMKVIINELSEGEEAGI
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCC
KSVTIEIEGVYAYGFLQNEKGTHRLVRISPFNANGKRQTSFAGVEVMPKLDEEVELEIPE
EEEEEEEEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEECCC
KDLEITTSRSGGAGGQNVNKVETAVRILHVPTGIAVRCTQERSQLQNKDKAMALLKSKLM
CCCEEEECCCCCCCCCCHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHH
VIALEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTEQETTDVDSVMNGEIDI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
FIQSLLRQNIEES
HHHHHHHHHCCCC
>Mature Secondary Structure
MRQLDEVKAQLEKLITWRGAIEDANLSLELYELDLDEEILSEAHNGLEKLKQELDRWETE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RLLSGIYDKEGAVLSINAGAGGTDAQDWAQMLLRMYTRWADNNGMKVIINELSEGEEAGI
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCC
KSVTIEIEGVYAYGFLQNEKGTHRLVRISPFNANGKRQTSFAGVEVMPKLDEEVELEIPE
EEEEEEEEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEECCC
KDLEITTSRSGGAGGQNVNKVETAVRILHVPTGIAVRCTQERSQLQNKDKAMALLKSKLM
CCCEEEECCCCCCCCCCHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHH
VIALEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTEQETTDVDSVMNGEIDI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
FIQSLLRQNIEES
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]