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The map label for this gene is holB [H]

Identifier: 124024914

GI number: 124024914

Start: 186200

End: 187159

Strand: Reverse

Name: holB [H]

Synonym: NATL1_02011

Alternate gene names: 124024914

Gene position: 187159-186200 (Counterclockwise)

Preceding gene: 124024915

Following gene: 124024909

Centisome position: 10.04

GC content: 28.23

Gene sequence:

>960_bases
ATGGACAATTTTCAAAATATATGTGGGCAAGATTTAGCAATTAAAATTTTAAAATCTGCTATTTCAAAAGAACATATTTC
TCATGCTTATTTATTTTCTGGTCCAGAGGGAGTTGGAAGAAAAAAAACTGCTAAAATTTTCATTAAATCTATTCTTGACC
AAAATCAGGAAAAAAAAAGTACAAAAAGAAAAATAAATAATAATAATCATCCTGACTTATTATGGGTAGAGCCATCTTAT
ATAGTCCAAGGTCAAAGTATTTCTCAGACAAAAGCAATTTTAGATGGTATAAGTATGAAATCGCCTCCGCAAATAAGGCT
AAATCAAATTAAAGAAATAATAGAATTTTTAGGGAAAAAGCCATTTGAATCAGAAAGAAGCATAGTAATAATTGAGGATA
TTGAAAGAATAAATGAATCTGCAGCAAATGCATTATTAAAAACTCTTGAAGAAACATGTACAGGATTATTTATTCTTATC
ACACAAAGACCAGAGAAATTATTATCAACTATTAGATCAAGATGTCAGATAGTCCCATTCATACGCCTGAATGATAATCA
AGTAAACAAAATTATTGAAAAATCAGAAGTTGTTCAAGAAATAGATGATATTCCTAGTGAAAAAATTAAAGAATTAATAG
ATTTCTCAAATGGATCTCCAGGGCGCTGTATAGTGAATCTTCAATTTTGGTTATCTTTTTCAACTTCATTAAGACAAAAG
TTAGAAATCCAATTAAATAATCCAATTGAGTTATTACAATTAGCCAAGGAAATCACAGATGAACTAAACATAGAGCAACA
ACTATGGCTTATTGATTTTCAACAGAATAGGGCATGGATAAAAGAAAAAAATTCAAATATTGTTGTACAACTTGAAGAGC
TTAGAAAACAATTATTGAAATTTGTGCAGCCTAGACTTGCTTGGGAAGTAACTTTATTAGAAATAAACTTTCTTAATTAA

Upstream 100 bases:

>100_bases
GAGGACAGTCACTGGAAAAAAATATCTGCTATTGACTCTAAAGAAAAAATCATATCCGAGATTAAATCTGAAATAAAGAA
ATTAATAAAAAACAAATAAA

Downstream 100 bases:

>100_bases
GCAATCACTCTTTCATCTGTATTATTTTTGAAAGAGTTGGTTTCTCTAGCTTGAATCATTGATGGTTGAATCTCACTTAT
TTTGTCCCCTGTTGGTAATT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MDNFQNICGQDLAIKILKSAISKEHISHAYLFSGPEGVGRKKTAKIFIKSILDQNQEKKSTKRKINNNNHPDLLWVEPSY
IVQGQSISQTKAILDGISMKSPPQIRLNQIKEIIEFLGKKPFESERSIVIIEDIERINESAANALLKTLEETCTGLFILI
TQRPEKLLSTIRSRCQIVPFIRLNDNQVNKIIEKSEVVQEIDDIPSEKIKELIDFSNGSPGRCIVNLQFWLSFSTSLRQK
LEIQLNNPIELLQLAKEITDELNIEQQLWLIDFQQNRAWIKEKNSNIVVQLEELRKQLLKFVQPRLAWEVTLLEINFLN

Sequences:

>Translated_319_residues
MDNFQNICGQDLAIKILKSAISKEHISHAYLFSGPEGVGRKKTAKIFIKSILDQNQEKKSTKRKINNNNHPDLLWVEPSY
IVQGQSISQTKAILDGISMKSPPQIRLNQIKEIIEFLGKKPFESERSIVIIEDIERINESAANALLKTLEETCTGLFILI
TQRPEKLLSTIRSRCQIVPFIRLNDNQVNKIIEKSEVVQEIDDIPSEKIKELIDFSNGSPGRCIVNLQFWLSFSTSLRQK
LEIQLNNPIELLQLAKEITDELNIEQQLWLIDFQQNRAWIKEKNSNIVVQLEELRKQLLKFVQPRLAWEVTLLEINFLN
>Mature_319_residues
MDNFQNICGQDLAIKILKSAISKEHISHAYLFSGPEGVGRKKTAKIFIKSILDQNQEKKSTKRKINNNNHPDLLWVEPSY
IVQGQSISQTKAILDGISMKSPPQIRLNQIKEIIEFLGKKPFESERSIVIIEDIERINESAANALLKTLEETCTGLFILI
TQRPEKLLSTIRSRCQIVPFIRLNDNQVNKIIEKSEVVQEIDDIPSEKIKELIDFSNGSPGRCIVNLQFWLSFSTSLRQK
LEIQLNNPIELLQLAKEITDELNIEQQLWLIDFQQNRAWIKEKNSNIVVQLEELRKQLLKFVQPRLAWEVTLLEINFLN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=174, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36824; Mature: 36824

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFQNICGQDLAIKILKSAISKEHISHAYLFSGPEGVGRKKTAKIFIKSILDQNQEKKS
CCCHHHHHCCHHHHHHHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHHHHCCCHHHHH
TKRKINNNNHPDLLWVEPSYIVQGQSISQTKAILDGISMKSPPQIRLNQIKEIIEFLGKK
HHHHCCCCCCCCEEEECCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
PFESERSIVIIEDIERINESAANALLKTLEETCTGLFILITQRPEKLLSTIRSRCQIVPF
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCEEEEE
IRLNDNQVNKIIEKSEVVQEIDDIPSEKIKELIDFSNGSPGRCIVNLQFWLSFSTSLRQK
EEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHHHH
LEIQLNNPIELLQLAKEITDELNIEQQLWLIDFQQNRAWIKEKNSNIVVQLEELRKQLLK
EEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHCCCCCEEEEHHHHHHHHHH
FVQPRLAWEVTLLEINFLN
HHCHHHEEEEEEEEEEECC
>Mature Secondary Structure
MDNFQNICGQDLAIKILKSAISKEHISHAYLFSGPEGVGRKKTAKIFIKSILDQNQEKKS
CCCHHHHHCCHHHHHHHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHHHHCCCHHHHH
TKRKINNNNHPDLLWVEPSYIVQGQSISQTKAILDGISMKSPPQIRLNQIKEIIEFLGKK
HHHHCCCCCCCCEEEECCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
PFESERSIVIIEDIERINESAANALLKTLEETCTGLFILITQRPEKLLSTIRSRCQIVPF
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCEEEEE
IRLNDNQVNKIIEKSEVVQEIDDIPSEKIKELIDFSNGSPGRCIVNLQFWLSFSTSLRQK
EEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHHHH
LEIQLNNPIELLQLAKEITDELNIEQQLWLIDFQQNRAWIKEKNSNIVVQLEELRKQLLK
EEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHCCCCCEEEEHHHHHHHHHH
FVQPRLAWEVTLLEINFLN
HHCHHHEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]