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The map label for this gene is cysH [H]

Identifier: 124024859

GI number: 124024859

Start: 143685

End: 144455

Strand: Reverse

Name: cysH [H]

Synonym: NATL1_01461

Alternate gene names: 124024859

Gene position: 144455-143685 (Counterclockwise)

Preceding gene: 124024866

Following gene: 124024858

Centisome position: 7.75

GC content: 38.26

Gene sequence:

>771_bases
ATGAAGAAAGAATCTAACGACAATTACATCAATGATTTGAGAAACGCCGCTCAATTACCTCAACCTGCTCTGATGAAATC
AATTCATGAGACAAGCGAAGATCTAGAGAAACTCTCCCCTCAGGATCAACTTCAATGGGCTTTTGAGGAATTTGATGAGA
ACTTTGTTGTTACCACAAGTTTTGGGATCCAATCCGCTGTCTTGCTTCACATGACGAAGGAATTGAAATCCAGTCAAAAG
CCAAAAGTTATTTGGGTTGATACTGGCTATTTGCCAAAAGAAACATACAATTATGCAGAAAAATTAACAACACTTTTCGA
ATTAAATTTAGTAGTTGCCCAAAGTTCCGTATCTCCAGCGAGAATGGAGGCCTTAAATGGTCGTCTATGGGAAACAGGAG
TACAAAAAGATCTTGAAAAATATCATCAGATTCGGAAAGTTGCACCTCTTGAAAAAGCTTTCTCAGACCTAAACGTTCTC
TGCTGGGCAAGTGGCGTGAGAAAAGGCCAAACCAAGAACAGACAATCAATGTCTAATATCGATTGCATTAGAGAACGTTT
AACGCTCCGACCACTTTTAAACTGGTCTAAAAAAGATATTTTCTATTACATGGAGAAAAATAAATTACCTCAACATCCAC
TATTTGAAAAAGGTTATTCCACTGTAGGAGATTGGCATTCAAGCGGAGCAGAAAATACTAATACCAAAGGAAGGTCAACG
AGATTTGGAGGCCTCAGTGAAGAATGTGGGATCCATTTAAATACGCAATAA

Upstream 100 bases:

>100_bases
GGATCAGATTTCAAATTGTTCAAGTCCATAGGGACTGGATCTAAGATGATTTCAAAGACCCTAAACAAACTTTGACTAAT
TCGGCATTCAAAACTCAAAC

Downstream 100 bases:

>100_bases
CAAGGAATAAACTCGTGTACTCAGAAGAAAATTTCCTAATGATTGGCAATACAAGATGGCATTGGGCAAGCAAAGTTAAA
AAAGATTGGAAATATTTTCA

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKKESNDNYINDLRNAAQLPQPALMKSIHETSEDLEKLSPQDQLQWAFEEFDENFVVTTSFGIQSAVLLHMTKELKSSQK
PKVIWVDTGYLPKETYNYAEKLTTLFELNLVVAQSSVSPARMEALNGRLWETGVQKDLEKYHQIRKVAPLEKAFSDLNVL
CWASGVRKGQTKNRQSMSNIDCIRERLTLRPLLNWSKKDIFYYMEKNKLPQHPLFEKGYSTVGDWHSSGAENTNTKGRST
RFGGLSEECGIHLNTQ

Sequences:

>Translated_256_residues
MKKESNDNYINDLRNAAQLPQPALMKSIHETSEDLEKLSPQDQLQWAFEEFDENFVVTTSFGIQSAVLLHMTKELKSSQK
PKVIWVDTGYLPKETYNYAEKLTTLFELNLVVAQSSVSPARMEALNGRLWETGVQKDLEKYHQIRKVAPLEKAFSDLNVL
CWASGVRKGQTKNRQSMSNIDCIRERLTLRPLLNWSKKDIFYYMEKNKLPQHPLFEKGYSTVGDWHSSGAENTNTKGRST
RFGGLSEECGIHLNTQ
>Mature_256_residues
MKKESNDNYINDLRNAAQLPQPALMKSIHETSEDLEKLSPQDQLQWAFEEFDENFVVTTSFGIQSAVLLHMTKELKSSQK
PKVIWVDTGYLPKETYNYAEKLTTLFELNLVVAQSSVSPARMEALNGRLWETGVQKDLEKYHQIRKVAPLEKAFSDLNVL
CWASGVRKGQTKNRQSMSNIDCIRERLTLRPLLNWSKKDIFYYMEKNKLPQHPLFEKGYSTVGDWHSSGAENTNTKGRST
RFGGLSEECGIHLNTQ

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=241, Percent_Identity=36.5145228215768, Blast_Score=172, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325425, Length=233, Percent_Identity=28.3261802575107, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 29379; Mature: 29379

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKESNDNYINDLRNAAQLPQPALMKSIHETSEDLEKLSPQDQLQWAFEEFDENFVVTTS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
FGIQSAVLLHMTKELKSSQKPKVIWVDTGYLPKETYNYAEKLTTLFELNLVVAQSSVSPA
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCHH
RMEALNGRLWETGVQKDLEKYHQIRKVAPLEKAFSDLNVLCWASGVRKGQTKNRQSMSNI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHH
DCIRERLTLRPLLNWSKKDIFYYMEKNKLPQHPLFEKGYSTVGDWHSSGAENTNTKGRST
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHCCCHHCCHHCCCCCCCCCCCCCC
RFGGLSEECGIHLNTQ
CCCCCCHHCCEEECCC
>Mature Secondary Structure
MKKESNDNYINDLRNAAQLPQPALMKSIHETSEDLEKLSPQDQLQWAFEEFDENFVVTTS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEC
FGIQSAVLLHMTKELKSSQKPKVIWVDTGYLPKETYNYAEKLTTLFELNLVVAQSSVSPA
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCHH
RMEALNGRLWETGVQKDLEKYHQIRKVAPLEKAFSDLNVLCWASGVRKGQTKNRQSMSNI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHH
DCIRERLTLRPLLNWSKKDIFYYMEKNKLPQHPLFEKGYSTVGDWHSSGAENTNTKGRST
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHCCCHHCCHHCCCCCCCCCCCCCC
RFGGLSEECGIHLNTQ
CCCCCCHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1463852 [H]