Click here to switch to the map view.

The map label for this gene is dcm [H]

Identifier: 124024750

GI number: 124024750

Start: 39055

End: 39972

Strand: Direct

Name: dcm [H]

Synonym: NATL1_00371

Alternate gene names: 124024750

Gene position: 39055-39972 (Clockwise)

Preceding gene: 124024748

Following gene: 124024754

Centisome position: 2.09

GC content: 31.37

Gene sequence:

>918_bases
ATGAGTAATAATTTTAGATTTATTGATCTTTTTGCTGGTATCGGAGGCATAAGAATACCTTTTGAAGAATTAGGCGGAGA
ATGTGTTTTTTCTTCTGAATGGGATAAATATTCACAAATTACCTATGAAGCAAATTTTGGTGAAATACCTAAAGGAGATA
TAACAAAAATTAATGCTGAAGATATTCCAAAACATGATTTACTTGTAGGTGGTTTTCCTTGCCAAGCATTTTCTCAAGCA
GGATTAAAAAAAGGTTTTCAAGATACAAGAGGAACTTTATTCTTTGATATTGCTCGTATTCTTAAATTCCATAAACCAAA
AGCGTTTTTATTAGAAAATGTTAAACATTTAAAAGGTCATGATAGTGGTAAAACATTTAAAACAATTATGAATGTTTTAA
GGGATATTGGTTATCAAACAATTGAATATAAAGTTCTTAACGCAAAGGATTTTGGTGTTCCCCAGAATCGAGAAAGAATT
TTTATTTTTGGTTATATTGATTTTATATTTTTCGAATTTCCTGAACCGAGTAAAGTTCAAACAAAGGTTGGCGATATCTT
AGATGATAATGTTCCGGATAAATTTACTCTCTCAGATAAGATGTGGACTAACGCAATAAGGAGAAAAGAAAATAATAGAA
AAAGAGGTTATGGATTCGGTTTTTCATTGTTCGATAAAGACTCTGAATATACCAGTACTATTTCAGCAAGATACTACAAG
GACGGTTCAGAGATTTGGATAGATCAAGATTCAAAAAATCCAAGAATGCTTACACCAAATGAAGCACGTAAACTGCAAGG
TTTTCCTGAAGGTTTTAAGATTCCCGTTAGTAATTTGCAGGCATATAAGCAATTTGGTAATTCAGTATCTGTGCCAGTAA
TTCGCGCTATTGCAGAGAGAATAGTTAAATTGCTTTAG

Upstream 100 bases:

>100_bases
TCTTTTTAAAAGTTAAAGCAAATTAGTTTCGCATGAAGAAAAATTGTATTCTCTAGACTTTTTTGGAAACCTGAAAAATT
AGTCTTTATTATAAAAACAT

Downstream 100 bases:

>100_bases
TGTATCTTTCCTCGATTAGTTCTTCCCAAAATGAGTTAATAGATCTCCACTGTGAATAATTTCTATTATTTCCTTGGTAC
ATGCCGCTATCGAAGAAAAT

Product: site-specific DNA methylase

Products: NA

Alternate protein names: M.DsaV; Cytosine-specific methyltransferase DsaV [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSNNFRFIDLFAGIGGIRIPFEELGGECVFSSEWDKYSQITYEANFGEIPKGDITKINAEDIPKHDLLVGGFPCQAFSQA
GLKKGFQDTRGTLFFDIARILKFHKPKAFLLENVKHLKGHDSGKTFKTIMNVLRDIGYQTIEYKVLNAKDFGVPQNRERI
FIFGYIDFIFFEFPEPSKVQTKVGDILDDNVPDKFTLSDKMWTNAIRRKENNRKRGYGFGFSLFDKDSEYTSTISARYYK
DGSEIWIDQDSKNPRMLTPNEARKLQGFPEGFKIPVSNLQAYKQFGNSVSVPVIRAIAERIVKLL

Sequences:

>Translated_305_residues
MSNNFRFIDLFAGIGGIRIPFEELGGECVFSSEWDKYSQITYEANFGEIPKGDITKINAEDIPKHDLLVGGFPCQAFSQA
GLKKGFQDTRGTLFFDIARILKFHKPKAFLLENVKHLKGHDSGKTFKTIMNVLRDIGYQTIEYKVLNAKDFGVPQNRERI
FIFGYIDFIFFEFPEPSKVQTKVGDILDDNVPDKFTLSDKMWTNAIRRKENNRKRGYGFGFSLFDKDSEYTSTISARYYK
DGSEIWIDQDSKNPRMLTPNEARKLQGFPEGFKIPVSNLQAYKQFGNSVSVPVIRAIAERIVKLL
>Mature_304_residues
SNNFRFIDLFAGIGGIRIPFEELGGECVFSSEWDKYSQITYEANFGEIPKGDITKINAEDIPKHDLLVGGFPCQAFSQAG
LKKGFQDTRGTLFFDIARILKFHKPKAFLLENVKHLKGHDSGKTFKTIMNVLRDIGYQTIEYKVLNAKDFGVPQNRERIF
IFGYIDFIFFEFPEPSKVQTKVGDILDDNVPDKFTLSDKMWTNAIRRKENNRKRGYGFGFSLFDKDSEYTSTISARYYKD
GSEIWIDQDSKNPRMLTPNEARKLQGFPEGFKIPVSNLQAYKQFGNSVSVPVIRAIAERIVKLL

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the DsaV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=352, Percent_Identity=40.625, Blast_Score=231, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 34955; Mature: 34824

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNFRFIDLFAGIGGIRIPFEELGGECVFSSEWDKYSQITYEANFGEIPKGDITKINAE
CCCCCEEEEEECCCCCCCCCHHHCCCEEEECCCCCCHHEEEEECCCCCCCCCCCEEECCC
DIPKHDLLVGGFPCQAFSQAGLKKGFQDTRGTLFFDIARILKFHKPKAFLLENVKHLKGH
CCCCCCEEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
DSGKTFKTIMNVLRDIGYQTIEYKVLNAKDFGVPQNRERIFIFGYIDFIFFEFPEPSKVQ
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEEEEEEEEEEECCCCCHHH
TKVGDILDDNVPDKFTLSDKMWTNAIRRKENNRKRGYGFGFSLFDKDSEYTSTISARYYK
HHHHHHHCCCCCCCEEECHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHEEEE
DGSEIWIDQDSKNPRMLTPNEARKLQGFPEGFKIPVSNLQAYKQFGNSVSVPVIRAIAER
CCCEEEEECCCCCCCEECCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
IVKLL
HHHHC
>Mature Secondary Structure 
SNNFRFIDLFAGIGGIRIPFEELGGECVFSSEWDKYSQITYEANFGEIPKGDITKINAE
CCCCEEEEEECCCCCCCCCHHHCCCEEEECCCCCCHHEEEEECCCCCCCCCCCEEECCC
DIPKHDLLVGGFPCQAFSQAGLKKGFQDTRGTLFFDIARILKFHKPKAFLLENVKHLKGH
CCCCCCEEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
DSGKTFKTIMNVLRDIGYQTIEYKVLNAKDFGVPQNRERIFIFGYIDFIFFEFPEPSKVQ
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEEEEEEEEEEECCCCCHHH
TKVGDILDDNVPDKFTLSDKMWTNAIRRKENNRKRGYGFGFSLFDKDSEYTSTISARYYK
HHHHHHHCCCCCCCEEECHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHEEEE
DGSEIWIDQDSKNPRMLTPNEARKLQGFPEGFKIPVSNLQAYKQFGNSVSVPVIRAIAER
CCCEEEEECCCCCCCEECCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
IVKLL
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7971279 [H]