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The map label for this gene is guaA

Identifier: 124024748

GI number: 124024748

Start: 35154

End: 36740

Strand: Direct

Name: guaA

Synonym: NATL1_00351

Alternate gene names: 124024748

Gene position: 35154-36740 (Clockwise)

Preceding gene: 124024747

Following gene: 124024750

Centisome position: 1.89

GC content: 37.11

Gene sequence:

>1587_bases
ATGGCTTCAATGATTTCAGCTGAAAAACGTAATCCAGCAATTGTAATTCTCGATTTTGGTTCTCAATATTCAGAATTAAT
TGCTCGAAGGATTAGAGAGACAGAGGTTTACTCATTAGTTATGAGTTACACAACATCTGCTGACAAATTACGTTCACTTA
AGCCTAAGGGAATTATTTTAAGCGGAGGTCCTGGATCTGTTTATGAAGAAGGTGCTCCATATTGTGACCCAGAGATTTTT
AATTTAGGAATTCCTGTTCTTGGTGTTTGTTATGGAATGCAATTAATGGTTCATGAGCTAGGAGGATCTGTAAAACCTGC
TACTGGGAAAGCCGAATATGGAAAGGCTCCTTTAGAAGTTGATGATCCAACAGCGTTATTAACGAATGTTATTAGTGGTT
CAACAATGTGGATGAGTCATGGTGATTCAGTTCAGAAATTACCCAAAGGATTTGTCAGATTAGCTCATACCTCGAATACT
TTAGAGGCTGCTATTGCTTTGCATGATAAGAGCTTTTATGGAGTGCAATTTCATCCCGAAGTTGTTCATTCCACCCATGG
AATGGTTGTAATAAGAAATTTTGTATATGATATTTGTTCTTGTGAGCCGGATTGGACAACAAATTTATTTATAGATGAAG
CTGTTTCTCAAGTGCAACAACAGGTAGGCGATAAAAAAGTTTTATTGGCTCTATCTGGTGGTGTTGATTCATCAACTCTT
GCATTTTTGTTAAACAAAGCAATAGGACCTCAATTGACGTGCATGTTTATTGATCAAGGTTTTATGAGAAAAGGTGAGCC
AGAATTTTTAATGTCTTTTTTTGATGAAAAGTTCAAGATTAATGTTGAATACATCAATGCCAGAGAAAGATTTATTTCAC
AATTAAAAGGAGTAACTGATCCTGAGCAAAAGCGTAAAATTATAGGTAGAGAATTTATTCGGGTTTTTGAAGAAGAGAGT
CTGCGATTAGGCCCTTTTGATTACTTGGCTCAAGGTACGCTTTATCCAGATGTAATTGAAAGTTCTGGAACAAATATTGA
TCCAAAAACTGGCGAACGAATAGCGGTTAAAATTAAAAGTCATCATAATGTAGGAGGGTTGCCAAAAGATTTGCAATTTA
AATTGGTAGAGCCCTTGAGACGTTTATTTAAAGATGAAGTCAGAAAAGTTGGTAAATCACTGGGATTGCCAGATGAGATT
GTTCGAAGACACCCATTTCCAGGTCCAGGATTGGCTATAAGAATTTTAGGAGAGGTTACTCATGAGAAACTAAATTGTTT
AAGAGACGCAGATTTAATTGTCAGAGAGGAGATTAATAATGCTGGCTTGTATAACAAAATTTGGCAGGCGTTCGCAGTTT
TATTGCCTGTGTATTCAGTGGGAGTAATGGGAGATCAAAGAACTTATGCGTGGCCAATTGTTGTTCGTTGTGTTTCTAGT
GAGGATGGGATGACTGCTGATTGGTCGCGTTTGCCGTATGCAGTTTTAGAGAAAATTTCTAACCGGATAGTTAACGAGGT
CGAGGGAGTTAATAGAGTTGTTTTAGATATAACAAGTAAACCCCCAGGAACTATTGAGTGGGAGTGA

Upstream 100 bases:

>100_bases
GATTAATTCTTTGGGGTTAATTCTTAAGCGATAGGCATATTCCTATTCTCTTAATTAGTCTATTTGATAAGAATGTTTTA
TAAGGATTCATCAGGTTGAT

Downstream 100 bases:

>100_bases
ATATAACTACTAAAACGTAGTTATAGGTTAAAAGTTGTAGTTCCACGTGAGTAGAAACGAAAAAATAGACAACACAATTG
CAATGGTTTTGAGAGACGTG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MASMISAEKRNPAIVILDFGSQYSELIARRIRETEVYSLVMSYTTSADKLRSLKPKGIILSGGPGSVYEEGAPYCDPEIF
NLGIPVLGVCYGMQLMVHELGGSVKPATGKAEYGKAPLEVDDPTALLTNVISGSTMWMSHGDSVQKLPKGFVRLAHTSNT
LEAAIALHDKSFYGVQFHPEVVHSTHGMVVIRNFVYDICSCEPDWTTNLFIDEAVSQVQQQVGDKKVLLALSGGVDSSTL
AFLLNKAIGPQLTCMFIDQGFMRKGEPEFLMSFFDEKFKINVEYINARERFISQLKGVTDPEQKRKIIGREFIRVFEEES
LRLGPFDYLAQGTLYPDVIESSGTNIDPKTGERIAVKIKSHHNVGGLPKDLQFKLVEPLRRLFKDEVRKVGKSLGLPDEI
VRRHPFPGPGLAIRILGEVTHEKLNCLRDADLIVREEINNAGLYNKIWQAFAVLLPVYSVGVMGDQRTYAWPIVVRCVSS
EDGMTADWSRLPYAVLEKISNRIVNEVEGVNRVVLDITSKPPGTIEWE

Sequences:

>Translated_528_residues
MASMISAEKRNPAIVILDFGSQYSELIARRIRETEVYSLVMSYTTSADKLRSLKPKGIILSGGPGSVYEEGAPYCDPEIF
NLGIPVLGVCYGMQLMVHELGGSVKPATGKAEYGKAPLEVDDPTALLTNVISGSTMWMSHGDSVQKLPKGFVRLAHTSNT
LEAAIALHDKSFYGVQFHPEVVHSTHGMVVIRNFVYDICSCEPDWTTNLFIDEAVSQVQQQVGDKKVLLALSGGVDSSTL
AFLLNKAIGPQLTCMFIDQGFMRKGEPEFLMSFFDEKFKINVEYINARERFISQLKGVTDPEQKRKIIGREFIRVFEEES
LRLGPFDYLAQGTLYPDVIESSGTNIDPKTGERIAVKIKSHHNVGGLPKDLQFKLVEPLRRLFKDEVRKVGKSLGLPDEI
VRRHPFPGPGLAIRILGEVTHEKLNCLRDADLIVREEINNAGLYNKIWQAFAVLLPVYSVGVMGDQRTYAWPIVVRCVSS
EDGMTADWSRLPYAVLEKISNRIVNEVEGVNRVVLDITSKPPGTIEWE
>Mature_527_residues
ASMISAEKRNPAIVILDFGSQYSELIARRIRETEVYSLVMSYTTSADKLRSLKPKGIILSGGPGSVYEEGAPYCDPEIFN
LGIPVLGVCYGMQLMVHELGGSVKPATGKAEYGKAPLEVDDPTALLTNVISGSTMWMSHGDSVQKLPKGFVRLAHTSNTL
EAAIALHDKSFYGVQFHPEVVHSTHGMVVIRNFVYDICSCEPDWTTNLFIDEAVSQVQQQVGDKKVLLALSGGVDSSTLA
FLLNKAIGPQLTCMFIDQGFMRKGEPEFLMSFFDEKFKINVEYINARERFISQLKGVTDPEQKRKIIGREFIRVFEEESL
RLGPFDYLAQGTLYPDVIESSGTNIDPKTGERIAVKIKSHHNVGGLPKDLQFKLVEPLRRLFKDEVRKVGKSLGLPDEIV
RRHPFPGPGLAIRILGEVTHEKLNCLRDADLIVREEINNAGLYNKIWQAFAVLLPVYSVGVMGDQRTYAWPIVVRCVSSE
DGMTADWSRLPYAVLEKISNRIVNEVEGVNRVVLDITSKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=553, Percent_Identity=37.7938517179023, Blast_Score=312, Evalue=5e-85,
Organism=Escherichia coli, GI1788854, Length=523, Percent_Identity=52.0076481835564, Blast_Score=549, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71992717, Length=574, Percent_Identity=36.7595818815331, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI71992710, Length=574, Percent_Identity=36.7595818815331, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI133901714, Length=574, Percent_Identity=36.7595818815331, Blast_Score=301, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6323873, Length=522, Percent_Identity=49.6168582375479, Blast_Score=503, Evalue=1e-143,
Organism=Drosophila melanogaster, GI281365319, Length=575, Percent_Identity=36.1739130434783, Blast_Score=306, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_PROM1 (A2BZD9)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001013864.1
- ProteinModelPortal:   A2BZD9
- SMR:   A2BZD9
- STRING:   A2BZD9
- GeneID:   4780186
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_00351
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   YENAICL
- ProtClustDB:   PRK00074
- BioCyc:   PMAR167555:NATL1_00351-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001962
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00733 Asn_synthase; PF00117 GATase; PF00958 GMP_synt_C

EC number: =6.3.5.2

Molecular weight: Translated: 58795; Mature: 58664

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 90-90 ACT_SITE 178-178 ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASMISAEKRNPAIVILDFGSQYSELIARRIRETEVYSLVMSYTTSADKLRSLKPKGIIL
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEE
SGGPGSVYEEGAPYCDPEIFNLGIPVLGVCYGMQLMVHELGGSVKPATGKAEYGKAPLEV
ECCCCCHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCC
DDPTALLTNVISGSTMWMSHGDSVQKLPKGFVRLAHTSNTLEAAIALHDKSFYGVQFHPE
CCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCHHEEEEEEECCCEECEEECHH
VVHSTHGMVVIRNFVYDICSCEPDWTTNLFIDEAVSQVQQQVGDKKVLLALSGGVDSSTL
HHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
AFLLNKAIGPQLTCMFIDQGFMRKGEPEFLMSFFDEKFKINVEYINARERFISQLKGVTD
HHHHHHCCCCCEEEEEECCHHCCCCCHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCCC
PEQKRKIIGREFIRVFEEESLRLGPFDYLAQGTLYPDVIESSGTNIDPKTGERIAVKIKS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCEEEEEEEC
HHNVGGLPKDLQFKLVEPLRRLFKDEVRKVGKSLGLPDEIVRRHPFPGPGLAIRILGEVT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEHHHHHH
HEKLNCLRDADLIVREEINNAGLYNKIWQAFAVLLPVYSVGVMGDQRTYAWPIVVRCVSS
HHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECC
EDGMTADWSRLPYAVLEKISNRIVNEVEGVNRVVLDITSKPPGTIEWE
CCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ASMISAEKRNPAIVILDFGSQYSELIARRIRETEVYSLVMSYTTSADKLRSLKPKGIIL
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEE
SGGPGSVYEEGAPYCDPEIFNLGIPVLGVCYGMQLMVHELGGSVKPATGKAEYGKAPLEV
ECCCCCHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCC
DDPTALLTNVISGSTMWMSHGDSVQKLPKGFVRLAHTSNTLEAAIALHDKSFYGVQFHPE
CCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCHHEEEEEEECCCEECEEECHH
VVHSTHGMVVIRNFVYDICSCEPDWTTNLFIDEAVSQVQQQVGDKKVLLALSGGVDSSTL
HHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
AFLLNKAIGPQLTCMFIDQGFMRKGEPEFLMSFFDEKFKINVEYINARERFISQLKGVTD
HHHHHHCCCCCEEEEEECCHHCCCCCHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCCC
PEQKRKIIGREFIRVFEEESLRLGPFDYLAQGTLYPDVIESSGTNIDPKTGERIAVKIKS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCEEEEEEEC
HHNVGGLPKDLQFKLVEPLRRLFKDEVRKVGKSLGLPDEIVRRHPFPGPGLAIRILGEVT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEHHHHHH
HEKLNCLRDADLIVREEINNAGLYNKIWQAFAVLLPVYSVGVMGDQRTYAWPIVVRCVSS
HHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECC
EDGMTADWSRLPYAVLEKISNRIVNEVEGVNRVVLDITSKPPGTIEWE
CCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA