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The map label for this gene is dus2 [H]

Identifier: 124024726

GI number: 124024726

Start: 17073

End: 18080

Strand: Reverse

Name: dus2 [H]

Synonym: NATL1_00131

Alternate gene names: 124024726

Gene position: 18080-17073 (Counterclockwise)

Preceding gene: 124024732

Following gene: 124024719

Centisome position: 0.97

GC content: 33.93

Gene sequence:

>1008_bases
ATGATTTCCAATTCTTTAAAAACAAATGAAATAGCTAACTACAGATTAAGTATTGCTCCAATGATGGATTGCACAGATAG
GCATTTTCGTGTCCTTATGCGCCAGATCACCAAAAAATCGCTTCTATACACTGAAATGATTGTGGCCCAAGCGCTCCATT
ACAGCAAAAACAGGAATAAATTATTAGATTTTGATGAGATTGAGCATCCTATTTCCATACAACTAGGAGGAGACAACCCA
AAACTTTTAGCTGAAGCAGCTCAAATGGCTGAAGATTGGGGCTATGACGAAATCAACTTAAACATTGGATGCCCAAGTCC
GAGAGTAAAATCTGGCAACTTTGGAGCTTGTCTTATGGGAAAACCAAAGATAGTGGCCAATTGTATTGAGAAAATGAAGG
AATCATGCAATATACCAATAACCGTCAAACACAGGCTTGGTATTGATAATCTTGACAGTGATGATTATCTTCTGACATTT
GTTGATACTTGTTCACTTGCAGGAGCAGACAGATTCATCATTCATGCAAGAAAAGCTTGGCTTAATGGATTAAATCCAAA
AGAAAATCGTACAATTCCACCTCTTCAATATGAAAGAGTCCAAAAATTAAAAAATCACAGGCCTGAATTAATTATTGAGC
TGAACGGTGGAATAAATACAATTAATGATTCTATTGAAGCTCTAAAAGTATTTGATGGGGCAATGGTAGGAAGAGCTGCA
TACTCTCATCCATTTCTTTGGACAAAAATTGATTCATTAATTTTTGGACAAAAAGAAAAATATTTATCAAGATCAAAAAT
AATTAAAAGGCTTATTCCTTTTGCTGAAAAGCATTTAGAAAATGATGGACGTCTTTGGCAAATTTCTAGACATATTTTAA
ATCTGATAGAAAATATTCCTAATGCAAAAATATTGAGACAAGAATTAAGTGAAAAATGTCAAACTCAGAAAGCTGATATT
TCTATTTTAAAAAAAATTGCCCAACAACTCGAAGATGCTGGGCAATAA

Upstream 100 bases:

>100_bases
AGTCCAAACATAAAAAATCTACGTTTCAAAGAAATTTCTCAATTCAGTGAATCTTAATAAAATTTCCTCCCTAAAAGTTG
AAAAAATTAATTCATAAAAA

Downstream 100 bases:

>100_bases
AAATCAATAAGCGAAATCTTTAAGATTCTGCGCCGCCATAATCTGCCGTAGAGTCGGCTCCCCCTTCAGGAGAAGCACCT
CTTCTTCTTCTACTACGACC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MISNSLKTNEIANYRLSIAPMMDCTDRHFRVLMRQITKKSLLYTEMIVAQALHYSKNRNKLLDFDEIEHPISIQLGGDNP
KLLAEAAQMAEDWGYDEINLNIGCPSPRVKSGNFGACLMGKPKIVANCIEKMKESCNIPITVKHRLGIDNLDSDDYLLTF
VDTCSLAGADRFIIHARKAWLNGLNPKENRTIPPLQYERVQKLKNHRPELIIELNGGINTINDSIEALKVFDGAMVGRAA
YSHPFLWTKIDSLIFGQKEKYLSRSKIIKRLIPFAEKHLENDGRLWQISRHILNLIENIPNAKILRQELSEKCQTQKADI
SILKKIAQQLEDAGQ

Sequences:

>Translated_335_residues
MISNSLKTNEIANYRLSIAPMMDCTDRHFRVLMRQITKKSLLYTEMIVAQALHYSKNRNKLLDFDEIEHPISIQLGGDNP
KLLAEAAQMAEDWGYDEINLNIGCPSPRVKSGNFGACLMGKPKIVANCIEKMKESCNIPITVKHRLGIDNLDSDDYLLTF
VDTCSLAGADRFIIHARKAWLNGLNPKENRTIPPLQYERVQKLKNHRPELIIELNGGINTINDSIEALKVFDGAMVGRAA
YSHPFLWTKIDSLIFGQKEKYLSRSKIIKRLIPFAEKHLENDGRLWQISRHILNLIENIPNAKILRQELSEKCQTQKADI
SILKKIAQQLEDAGQ
>Mature_335_residues
MISNSLKTNEIANYRLSIAPMMDCTDRHFRVLMRQITKKSLLYTEMIVAQALHYSKNRNKLLDFDEIEHPISIQLGGDNP
KLLAEAAQMAEDWGYDEINLNIGCPSPRVKSGNFGACLMGKPKIVANCIEKMKESCNIPITVKHRLGIDNLDSDDYLLTF
VDTCSLAGADRFIIHARKAWLNGLNPKENRTIPPLQYERVQKLKNHRPELIIELNGGINTINDSIEALKVFDGAMVGRAA
YSHPFLWTKIDSLIFGQKEKYLSRSKIIKRLIPFAEKHLENDGRLWQISRHILNLIENIPNAKILRQELSEKCQTQKADI
SILKKIAQQLEDAGQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=238, Percent_Identity=26.4705882352941, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40807366, Length=320, Percent_Identity=24.375, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI145693211, Length=327, Percent_Identity=44.6483180428135, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI1789660, Length=272, Percent_Identity=27.2058823529412, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788462, Length=289, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=179, Percent_Identity=26.8156424581006, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=206, Percent_Identity=27.6699029126214, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24580595, Length=295, Percent_Identity=22.3728813559322, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19920448, Length=295, Percent_Identity=22.3728813559322, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38131; Mature: 38131

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISNSLKTNEIANYRLSIAPMMDCTDRHFRVLMRQITKKSLLYTEMIVAQALHYSKNRNK
CCCCCCCCCHHCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LLDFDEIEHPISIQLGGDNPKLLAEAAQMAEDWGYDEINLNIGCPSPRVKSGNFGACLMG
CCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECC
KPKIVANCIEKMKESCNIPITVKHRLGIDNLDSDDYLLTFVDTCSLAGADRFIIHARKAW
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEHHHCCCCCCHHHHEEHHHH
LNGLNPKENRTIPPLQYERVQKLKNHRPELIIELNGGINTINDSIEALKVFDGAMVGRAA
HCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
YSHPFLWTKIDSLIFGQKEKYLSRSKIIKRLIPFAEKHLENDGRLWQISRHILNLIENIP
HCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
NAKILRQELSEKCQTQKADISILKKIAQQLEDAGQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISNSLKTNEIANYRLSIAPMMDCTDRHFRVLMRQITKKSLLYTEMIVAQALHYSKNRNK
CCCCCCCCCHHCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LLDFDEIEHPISIQLGGDNPKLLAEAAQMAEDWGYDEINLNIGCPSPRVKSGNFGACLMG
CCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECC
KPKIVANCIEKMKESCNIPITVKHRLGIDNLDSDDYLLTFVDTCSLAGADRFIIHARKAW
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEHHHCCCCCCHHHHEEHHHH
LNGLNPKENRTIPPLQYERVQKLKNHRPELIIELNGGINTINDSIEALKVFDGAMVGRAA
HCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
YSHPFLWTKIDSLIFGQKEKYLSRSKIIKRLIPFAEKHLENDGRLWQISRHILNLIENIP
HCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
NAKILRQELSEKCQTQKADISILKKIAQQLEDAGQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]