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The map label for this gene is purF [H]

Identifier: 124024716

GI number: 124024716

Start: 4597

End: 6054

Strand: Direct

Name: purF [H]

Synonym: NATL1_00031

Alternate gene names: 124024716

Gene position: 4597-6054 (Clockwise)

Preceding gene: 124024715

Following gene: 124024720

Centisome position: 0.25

GC content: 33.06

Gene sequence:

>1458_bases
ATGTGTGGAATAGTAGGTGTTGTTTCAACTGATCAATGTAATCAACAAATTTACGATAGTTTGCTATTGCTTCAGCATAG
AGGTCAAGATTCCACTGGTATAGCAACAATGGATGGCAGTGTTTTTCATATTAATAAATCTAAAGGTCAAGTTCGAGAAG
CTTATAGAACTAGAGATATGAGAGCTTTGATCGGAAGATCAGGATTGGGACATGTACGTTATGCAACTAAAGGTGCTGCT
CATAGAGAAGAAGAAGCACAACCTTTTTATGTCAATGCTCCTTACGGAATTATTCTTGTTCATAATGGAAACTTAACAAA
TACAAGAGAGCTAGAAAAAGAACTTTTTAAAGTTGATAAAAGACATACAAATACATCGAGCGATACTGAAATGTTATTAA
ATGTTTTGGCAACAGAATTAAATAGTGAAGTAAAAGGAAAAGATGTAGATCCAGAGGATCTTTTTAATGCTGTTAAAAGA
CTTCACTGTAGAGTAGAGGGATCCTATGCTTCAATCGCTTTAATAGCAGGTCATGGTCTTTTAGCATTTAGAGATCCTTT
TGGAATTCGCCCTTTGGTTATTGGTAAAAGGGTAAAAGAAAATAACAAACCTGAGTGGGTAATCGCTAGTGAATCGCTAG
TCATTGAAAACAATGATTATGTAATTGTTAGAGATGTTGAACCAGGTGAGGCGATATTTATAACTTCAAATGGTGAGTTT
TTTTCTAAACAATGCTCAGATAATCCTCAACTATTCCCCTGTTCATTTGAATATGTTTATTTAGCAAGACCTGACTCAAT
AATGAATGGTATTTCTGTTTATGAGTCAAGATTAAGAATGGGGGATTTATTAGCAGAAACTATAAAAAAACAAATTTCTC
TTGGTGATATAGATGTCGTAATGCCAATTCCTGATTCTTCTAGACCTGCTGCAATGCAGGTAGCAAGACAGTTAGGGATT
GAATATAGGGAGGGTTTTTTCAAGAATCGTTATGTTGGTAGAACATTTATAATGCCTGGTCAATCTCTAAGAAAACGTTC
AGTGCGTCAGAAATTAAATGCAATGAGTACTGAATTTAAAAATAAAAATGTATTGATAGTTGATGATTCAGTGGTTAGAG
GAACGACATCTCAGCAAATAGTTCAAATGGCAAGAAGTGCAGGGGCTAATAAAGTTACTTTTACATCTGCTGCACCACCA
ATAAGATATCCACATGTTTATGGAATTAATATGCCAAGTAGAAATGAATTGATTGCTTATAATAGAGATATAAATCAAAT
AGAAAATAATTTATTTATTGATAAAATGATTTATCAAGAGGTAAATGATCTTACTCAGGCTATTACGCAAAATTCTAAAA
TTAAGGAATTAGATTTATCATGCTTTACAGGAAAATATATTACAGGAACAGTCACTGATGAATATTTAACTTGGGTTGAA
GAAACATCTTTGTCTTAA

Upstream 100 bases:

>100_bases
ACATATAAAGATGCCATCCATAAAAAAATAACTAAATAAAATCATTTATGATTTATAATCCTAAAACTACATTTATTTTA
CTTAACCTAGGAATATAATT

Downstream 100 bases:

>100_bases
TTTTTGTCTGGTACTTTCATTGAAAAAGTAGATTTTAAAAACTCATCATTGTTTAATTCTATATTTAATTTGTTCTCTTT
TCTATATGAACTATTATCAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MCGIVGVVSTDQCNQQIYDSLLLLQHRGQDSTGIATMDGSVFHINKSKGQVREAYRTRDMRALIGRSGLGHVRYATKGAA
HREEEAQPFYVNAPYGIILVHNGNLTNTRELEKELFKVDKRHTNTSSDTEMLLNVLATELNSEVKGKDVDPEDLFNAVKR
LHCRVEGSYASIALIAGHGLLAFRDPFGIRPLVIGKRVKENNKPEWVIASESLVIENNDYVIVRDVEPGEAIFITSNGEF
FSKQCSDNPQLFPCSFEYVYLARPDSIMNGISVYESRLRMGDLLAETIKKQISLGDIDVVMPIPDSSRPAAMQVARQLGI
EYREGFFKNRYVGRTFIMPGQSLRKRSVRQKLNAMSTEFKNKNVLIVDDSVVRGTTSQQIVQMARSAGANKVTFTSAAPP
IRYPHVYGINMPSRNELIAYNRDINQIENNLFIDKMIYQEVNDLTQAITQNSKIKELDLSCFTGKYITGTVTDEYLTWVE
ETSLS

Sequences:

>Translated_485_residues
MCGIVGVVSTDQCNQQIYDSLLLLQHRGQDSTGIATMDGSVFHINKSKGQVREAYRTRDMRALIGRSGLGHVRYATKGAA
HREEEAQPFYVNAPYGIILVHNGNLTNTRELEKELFKVDKRHTNTSSDTEMLLNVLATELNSEVKGKDVDPEDLFNAVKR
LHCRVEGSYASIALIAGHGLLAFRDPFGIRPLVIGKRVKENNKPEWVIASESLVIENNDYVIVRDVEPGEAIFITSNGEF
FSKQCSDNPQLFPCSFEYVYLARPDSIMNGISVYESRLRMGDLLAETIKKQISLGDIDVVMPIPDSSRPAAMQVARQLGI
EYREGFFKNRYVGRTFIMPGQSLRKRSVRQKLNAMSTEFKNKNVLIVDDSVVRGTTSQQIVQMARSAGANKVTFTSAAPP
IRYPHVYGINMPSRNELIAYNRDINQIENNLFIDKMIYQEVNDLTQAITQNSKIKELDLSCFTGKYITGTVTDEYLTWVE
ETSLS
>Mature_485_residues
MCGIVGVVSTDQCNQQIYDSLLLLQHRGQDSTGIATMDGSVFHINKSKGQVREAYRTRDMRALIGRSGLGHVRYATKGAA
HREEEAQPFYVNAPYGIILVHNGNLTNTRELEKELFKVDKRHTNTSSDTEMLLNVLATELNSEVKGKDVDPEDLFNAVKR
LHCRVEGSYASIALIAGHGLLAFRDPFGIRPLVIGKRVKENNKPEWVIASESLVIENNDYVIVRDVEPGEAIFITSNGEF
FSKQCSDNPQLFPCSFEYVYLARPDSIMNGISVYESRLRMGDLLAETIKKQISLGDIDVVMPIPDSSRPAAMQVARQLGI
EYREGFFKNRYVGRTFIMPGQSLRKRSVRQKLNAMSTEFKNKNVLIVDDSVVRGTTSQQIVQMARSAGANKVTFTSAAPP
IRYPHVYGINMPSRNELIAYNRDINQIENNLFIDKMIYQEVNDLTQAITQNSKIKELDLSCFTGKYITGTVTDEYLTWVE
ETSLS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=478, Percent_Identity=36.4016736401674, Blast_Score=258, Evalue=9e-69,
Organism=Escherichia coli, GI1788651, Length=482, Percent_Identity=51.8672199170125, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=31.390134529148, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=486, Percent_Identity=32.9218106995885, Blast_Score=224, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323958, Length=489, Percent_Identity=47.4437627811861, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24659598, Length=487, Percent_Identity=34.7022587268994, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI28573187, Length=509, Percent_Identity=34.3811394891945, Blast_Score=251, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24659604, Length=481, Percent_Identity=34.7193347193347, Blast_Score=251, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54530; Mature: 54530

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGVVSTDQCNQQIYDSLLLLQHRGQDSTGIATMDGSVFHINKSKGQVREAYRTRDM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHH
RALIGRSGLGHVRYATKGAAHREEEAQPFYVNAPYGIILVHNGNLTNTRELEKELFKVDK
HHHHCCCCCCCEEEECCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHTNTSSDTEMLLNVLATELNSEVKGKDVDPEDLFNAVKRLHCRVEGSYASIALIAGHGL
HCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEECCCEEEEEEECCCE
LAFRDPFGIRPLVIGKRVKENNKPEWVIASESLVIENNDYVIVRDVEPGEAIFITSNGEF
EEECCCCCCCCEEECCHHHCCCCCCEEEECCEEEEECCCEEEEEECCCCCEEEEECCCCH
FSKQCSDNPQLFPCSFEYVYLARPDSIMNGISVYESRLRMGDLLAETIKKQISLGDIDVV
HHHHCCCCCCEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MPIPDSSRPAAMQVARQLGIEYREGFFKNRYVGRTFIMPGQSLRKRSVRQKLNAMSTEFK
EECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHC
NKNVLIVDDSVVRGTTSQQIVQMARSAGANKVTFTSAAPPIRYPHVYGINMPSRNELIAY
CCCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCEEEE
NRDINQIENNLFIDKMIYQEVNDLTQAITQNSKIKELDLSCFTGKYITGTVTDEYLTWVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCEEEEEECHHHHHHHH
ETSLS
HHCCC
>Mature Secondary Structure
MCGIVGVVSTDQCNQQIYDSLLLLQHRGQDSTGIATMDGSVFHINKSKGQVREAYRTRDM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHH
RALIGRSGLGHVRYATKGAAHREEEAQPFYVNAPYGIILVHNGNLTNTRELEKELFKVDK
HHHHCCCCCCCEEEECCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHTNTSSDTEMLLNVLATELNSEVKGKDVDPEDLFNAVKRLHCRVEGSYASIALIAGHGL
HCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEECCCEEEEEEECCCE
LAFRDPFGIRPLVIGKRVKENNKPEWVIASESLVIENNDYVIVRDVEPGEAIFITSNGEF
EEECCCCCCCCEEECCHHHCCCCCCEEEECCEEEEECCCEEEEEECCCCCEEEEECCCCH
FSKQCSDNPQLFPCSFEYVYLARPDSIMNGISVYESRLRMGDLLAETIKKQISLGDIDVV
HHHHCCCCCCEEEECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MPIPDSSRPAAMQVARQLGIEYREGFFKNRYVGRTFIMPGQSLRKRSVRQKLNAMSTEFK
EECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHC
NKNVLIVDDSVVRGTTSQQIVQMARSAGANKVTFTSAAPPIRYPHVYGINMPSRNELIAY
CCCEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCEEEE
NRDINQIENNLFIDKMIYQEVNDLTQAITQNSKIKELDLSCFTGKYITGTVTDEYLTWVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCEEEEEECHHHHHHHH
ETSLS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10873488; 11248100 [H]