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The map label for this gene is trmJ [H]

Identifier: 124028494

GI number: 124028494

Start: 1648190

End: 1648909

Strand: Reverse

Name: trmJ [H]

Synonym: Hbut_1655

Alternate gene names: 124028494

Gene position: 1648909-1648190 (Counterclockwise)

Preceding gene: 124028495

Following gene: 124028493

Centisome position: 98.91

GC content: 53.19

Gene sequence:

>720_bases
TTGCAGAGTCTTGGCCCGCCAAGGGTGAGGCTGGTAGTTGTCGGTGTTGAGGGCGAGATTAACCTAGGCTTTATAGCTAG
GCTTGCTGCCAACTTTGCAATAGACGAGTTCTACCTTGTATCGCCGGTTGCGAGCCCTGAAAGCAGTGAGGCTAGGCGAT
TTGCCGCCAATGGAGTCTACATGCTGGATCGTGTAATAGTGGTTGATAGTCTTGAAGAGGCGTTGAAGGGTGTGGATCTA
GCGGTATGTACGAGTGCAAAGATAGGCCAGGCGTCGGATGTGCTTCGCCACGCTGTCACCCCCCGCGAGTTCGCCGAGGA
GATAGCGCCACGCTATGAGAGTGTTGCAGTCGTGTTTGGGAGGGAAAGTGTAGGACTAACAAGGGATGAACTGGCAAAAT
GCCACCTTCTAGTCCACATACCAGCTAACCCAAACTATCCCGTCCTAAACCTAAGCCACGCAGTAGCAATACTCCTCTAC
GAACTCTACAACACGCGGCGCGGAGAAAAACAACTTCACGAGCCAGCCCGCGGCGAGACACTGGAACGAATATACGAGCA
CCTATCATGGCTCGCCGAGAAGCTCGTCGACAGGGAGAGAGTTAGCCAGGTCAAGGCGGCACTTAAGCACATACTGTGGC
GCTGCAGTATTACGGGTGGAGAGGCATCAATGCTATACTATTTTGCTAAGAAGCTGAGAAAGCGGCTTGAAGCGTGCTAG

Upstream 100 bases:

>100_bases
GTGATGATAGGGAGTTGGTTGGAAAGCTTAGGGATAGGGGGTTTGGTGTTGAGAGATACAAAATACACTTCTTCTTCGAG
GACATAGTGGGTGTTAGGGC

Downstream 100 bases:

>100_bases
GCATGAGGGAGCTGCAGCATTGGTTAAGCTACTACTCACAACCAATCCGGGTATAGAGGATATCGCTGCACACGAAGCAG
AAGCCAAGATCTCAGCTAGG

Product: rRNA methylase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MQSLGPPRVRLVVVGVEGEINLGFIARLAANFAIDEFYLVSPVASPESSEARRFAANGVYMLDRVIVVDSLEEALKGVDL
AVCTSAKIGQASDVLRHAVTPREFAEEIAPRYESVAVVFGRESVGLTRDELAKCHLLVHIPANPNYPVLNLSHAVAILLY
ELYNTRRGEKQLHEPARGETLERIYEHLSWLAEKLVDRERVSQVKAALKHILWRCSITGGEASMLYYFAKKLRKRLEAC

Sequences:

>Translated_239_residues
MQSLGPPRVRLVVVGVEGEINLGFIARLAANFAIDEFYLVSPVASPESSEARRFAANGVYMLDRVIVVDSLEEALKGVDL
AVCTSAKIGQASDVLRHAVTPREFAEEIAPRYESVAVVFGRESVGLTRDELAKCHLLVHIPANPNYPVLNLSHAVAILLY
ELYNTRRGEKQLHEPARGETLERIYEHLSWLAEKLVDRERVSQVKAALKHILWRCSITGGEASMLYYFAKKLRKRLEAC
>Mature_239_residues
MQSLGPPRVRLVVVGVEGEINLGFIARLAANFAIDEFYLVSPVASPESSEARRFAANGVYMLDRVIVVDSLEEALKGVDL
AVCTSAKIGQASDVLRHAVTPREFAEEIAPRYESVAVVFGRESVGLTRDELAKCHLLVHIPANPNYPVLNLSHAVAILLY
ELYNTRRGEKQLHEPARGETLERIYEHLSWLAEKLVDRERVSQVKAALKHILWRCSITGGEASMLYYFAKKLRKRLEAC

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=230, Percent_Identity=34.3478260869565, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790865, Length=176, Percent_Identity=30.1136363636364, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26697; Mature: 26697

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLGPPRVRLVVVGVEGEINLGFIARLAANFAIDEFYLVSPVASPESSEARRFAANGVY
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCHH
MLDRVIVVDSLEEALKGVDLAVCTSAKIGQASDVLRHAVTPREFAEEIAPRYESVAVVFG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHCCCHHHEEEEEC
RESVGLTRDELAKCHLLVHIPANPNYPVLNLSHAVAILLYELYNTRRGEKQLHEPARGET
CCCCCCCHHHHHEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCHH
LERIYEHLSWLAEKLVDRERVSQVKAALKHILWRCSITGGEASMLYYFAKKLRKRLEAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSLGPPRVRLVVVGVEGEINLGFIARLAANFAIDEFYLVSPVASPESSEARRFAANGVY
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCHH
MLDRVIVVDSLEEALKGVDLAVCTSAKIGQASDVLRHAVTPREFAEEIAPRYESVAVVFG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHCCCHHHEEEEEC
RESVGLTRDELAKCHLLVHIPANPNYPVLNLSHAVAILLYELYNTRRGEKQLHEPARGET
CCCCCCCHHHHHEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCHH
LERIYEHLSWLAEKLVDRERVSQVKAALKHILWRCSITGGEASMLYYFAKKLRKRLEAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA