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The map label for this gene is cobB2 [H]

Identifier: 124028307

GI number: 124028307

Start: 1465477

End: 1466253

Strand: Reverse

Name: cobB2 [H]

Synonym: Hbut_1458

Alternate gene names: 124028307

Gene position: 1466253-1465477 (Counterclockwise)

Preceding gene: 124028311

Following gene: 124028303

Centisome position: 87.95

GC content: 59.33

Gene sequence:

>777_bases
TTGGAGGCATGGCTTCCTATGGTTGGACTTGTGGAGGATATTCGCCGGGCTGCGGAGCTGCTTGTACAGTATCGGCCCTT
CATAGCGTTCACCGGGGCTGGCATATCGGCTGAGAGTGGTGTGCCGACCTTCAGGGGGCGTGGTGGGCTATGGGAGAGGT
TCAGGCCGGAAGAGCTTGCTACGCCGGAGGCGTTTGAGAGGAACCCCCGGCTTGTCTGGGAATGGTACAGGTGGAGGATG
GAGATAATCTACAAGGCCAGGCCGAATCCCGCGCACTACGCGCTAGTTGAGCTTGAGAGGCTCGGGCTCATAAGATGCGT
GGTGACGCAGAACGTTGACGGTCTGCACCAGCGTGCTGGGCAGCACTGTGTTGTAGAACTCCACGGGAATATTTGGCGGG
CCAGGTGCATGCGCTGCAGTTACAAGCTGGTATTCAGGGAGCCCCCAAGAGAGGTCCCGCCACGCTGTCCGCGCTGTGGC
GGCCTCCTGCGCCCCGACGTGGTCTGGTTTGGAGAGCCTCTACCCGAGGAGGCTTGGAGAACAGCGGTCGAGCTTGCAGA
GTCTAGCCAGGGCATTCTCGTGGTTGGAACCAGCGGTGTCGTTATGCCTGCAGGCTATATCCCGCACATCGTGAAACAGC
ATGGCGGCTACGTGGTCGAAGTCAACATAGAGGAGTCAGCGGTAACCCCCATAGCTGATGTATTCCTAAAAGGTAGGGCG
GGTGAGGTGCTGCCAAGACTCGTTGAGGAAGCTAGAAAGCTGCTGGCCCACGGTTAG

Upstream 100 bases:

>100_bases
CCCGAGCGGGCCGTCTTACGCAGCGGCCCTGCTCCGCGGAGCCAGGGTGTACTTTGGGCTGCGTGGGGTATATGGCTGCT
ACTCGTGGACTGGTGTTCTC

Downstream 100 bases:

>100_bases
CCCTGCGTGGAGGGTTTGCTAGTCCATGCTGGTGACTGGTAGGTACAACGGCTTCTCGCTCCCATCCTCGAATACGAGTA
CCAGTGTTATGTACTCGGAC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2 [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MEAWLPMVGLVEDIRRAAELLVQYRPFIAFTGAGISAESGVPTFRGRGGLWERFRPEELATPEAFERNPRLVWEWYRWRM
EIIYKARPNPAHYALVELERLGLIRCVVTQNVDGLHQRAGQHCVVELHGNIWRARCMRCSYKLVFREPPREVPPRCPRCG
GLLRPDVVWFGEPLPEEAWRTAVELAESSQGILVVGTSGVVMPAGYIPHIVKQHGGYVVEVNIEESAVTPIADVFLKGRA
GEVLPRLVEEARKLLAHG

Sequences:

>Translated_258_residues
MEAWLPMVGLVEDIRRAAELLVQYRPFIAFTGAGISAESGVPTFRGRGGLWERFRPEELATPEAFERNPRLVWEWYRWRM
EIIYKARPNPAHYALVELERLGLIRCVVTQNVDGLHQRAGQHCVVELHGNIWRARCMRCSYKLVFREPPREVPPRCPRCG
GLLRPDVVWFGEPLPEEAWRTAVELAESSQGILVVGTSGVVMPAGYIPHIVKQHGGYVVEVNIEESAVTPIADVFLKGRA
GEVLPRLVEEARKLLAHG
>Mature_258_residues
MEAWLPMVGLVEDIRRAAELLVQYRPFIAFTGAGISAESGVPTFRGRGGLWERFRPEELATPEAFERNPRLVWEWYRWRM
EIIYKARPNPAHYALVELERLGLIRCVVTQNVDGLHQRAGQHCVVELHGNIWRARCMRCSYKLVFREPPREVPPRCPRCG
GLLRPDVVWFGEPLPEEAWRTAVELAESSQGILVVGTSGVVMPAGYIPHIVKQHGGYVVEVNIEESAVTPIADVFLKGRA
GEVLPRLVEEARKLLAHG

Specific function: Modulates the activity of chromatin proteins, thereby having an effect on transcription per subunit [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=255, Percent_Identity=39.6078431372549, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI6912664, Length=268, Percent_Identity=39.1791044776119, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI13787215, Length=251, Percent_Identity=40.2390438247012, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI300797577, Length=247, Percent_Identity=32.3886639676113, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI7657575, Length=220, Percent_Identity=30, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI300797597, Length=234, Percent_Identity=32.0512820512821, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI63054862, Length=214, Percent_Identity=34.1121495327103, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI6912660, Length=218, Percent_Identity=33.4862385321101, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI6912662, Length=270, Percent_Identity=28.5185185185185, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI13775602, Length=198, Percent_Identity=32.8282828282828, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI13775600, Length=198, Percent_Identity=32.8282828282828, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI300797705, Length=193, Percent_Identity=32.6424870466321, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI7706712, Length=207, Percent_Identity=29.4685990338164, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI215982798, Length=160, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI308199517, Length=236, Percent_Identity=40.6779661016949, Blast_Score=151, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17541892, Length=241, Percent_Identity=32.3651452282158, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71990482, Length=276, Percent_Identity=27.536231884058, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17567771, Length=273, Percent_Identity=27.1062271062271, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71990487, Length=278, Percent_Identity=27.3381294964029, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17505510, Length=256, Percent_Identity=25.390625, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325242, Length=214, Percent_Identity=30.3738317757009, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28571445, Length=286, Percent_Identity=27.6223776223776, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648389, Length=195, Percent_Identity=30.7692307692308, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17137536, Length=226, Percent_Identity=28.7610619469027, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28571443, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28571441, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24645650, Length=256, Percent_Identity=25.390625, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24650933, Length=257, Percent_Identity=26.8482490272374, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 29191; Mature: 29191

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAWLPMVGLVEDIRRAAELLVQYRPFIAFTGAGISAESGVPTFRGRGGLWERFRPEELA
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHCC
TPEAFERNPRLVWEWYRWRMEIIYKARPNPAHYALVELERLGLIRCVVTQNVDGLHQRAG
CCHHHCCCCCEEHHHHHHEEHHEEECCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHCC
QHCVVELHGNIWRARCMRCSYKLVFREPPREVPPRCPRCGGLLRPDVVWFGEPLPEEAWR
CEEEEEECCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHH
TAVELAESSQGILVVGTSGVVMPAGYIPHIVKQHGGYVVEVNIEESAVTPIADVFLKGRA
HHHHHHHCCCCEEEEECCCEEECCCHHHHHHHHCCCEEEEEECCCHHCCHHHHHHHCCCC
GEVLPRLVEEARKLLAHG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAWLPMVGLVEDIRRAAELLVQYRPFIAFTGAGISAESGVPTFRGRGGLWERFRPEELA
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHCC
TPEAFERNPRLVWEWYRWRMEIIYKARPNPAHYALVELERLGLIRCVVTQNVDGLHQRAG
CCHHHCCCCCEEHHHHHHEEHHEEECCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHCC
QHCVVELHGNIWRARCMRCSYKLVFREPPREVPPRCPRCGGLLRPDVVWFGEPLPEEAWR
CEEEEEECCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHH
TAVELAESSQGILVVGTSGVVMPAGYIPHIVKQHGGYVVEVNIEESAVTPIADVFLKGRA
HHHHHHHCCCCEEEEECCCEEECCCHHHHHHHHCCCEEEEEECCCHHCCHHHHHHHCCCC
GEVLPRLVEEARKLLAHG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]