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The map label for this gene is capL [H]

Identifier: 124028269

GI number: 124028269

Start: 1423394

End: 1424755

Strand: Direct

Name: capL [H]

Synonym: Hbut_1420

Alternate gene names: 124028269

Gene position: 1423394-1424755 (Clockwise)

Preceding gene: 124028263

Following gene: 124028270

Centisome position: 85.38

GC content: 57.49

Gene sequence:

>1362_bases
TTGCAGCCCGGCAGCTGGCTAGACGAGCTTAGGGAGAAGCTACGGGAAAACCGTGTAGTGGTAGGCGTTGTAGGCCTCGG
CTACGTGGGGCTCCCGCTCTCCCTCCTCTACGCCTCCAAGGGGTTCCGCGTCATAGGCTACGATATCGACCAGGGAAGGG
TTGAGGCAATACGTAGGGGGCGCTCCTACATAAACTACATTCCCGACCACGAGGTAGCCAGGGTCGTCAAGGAGGGCCTA
CTCGAAGCCTACACGAGCCCGGAGCCGCTCCACGAAGCAGACTTCATACTCGTAACGGTGCCTACACCGCTCCGCTGCAA
CGGCCTACCAGACTTGAGCTATGTGAGGAGCGCTGCTAGGAGTGTTGGCAGGGTGCTCCGCCGCGGCCAGGTAGTGGTAT
TGGAGAGTTCCACGTACCCCGGCACGACGAGGGAGGTGCTGGCGCCGATACTTGAGGAGGAGAGCGGCCTACGCGTCGTG
AGGGACTTTGGTGTAGCCTACTCCCCGGAGAGGATCAATCCCGGCGATACCAGGTATAAGCTAGAGGATATACCCAAGGT
AGTCGGCGGCATCACACCTGAGATAACCGATATAGTGGCAGAGTTCTACTCAAAAGCGTTCAAGTATGTCGTAAAGGTGA
AGGATTGTAAGACGGCTGAGGCCACCAAGATGCTCGAGAACATGTTCCGCTACATAAACATAGCACTTGTGAACGAGCTT
GCAGTAGCCTTCGAGAAGATGGGGATAAATGTCTGGGAGGTCATAGAGGCAGCGAAGACAAAACCATTCGGCTTCATGGC
ATTCTACCCCGGGCCCGGTGTTGGCGGCCACTGCATCCCCGTAGACCCATTCTACCTCCGCTACAGCGCCTGGCGCCACG
GCGCAGTCCTACGCCTCGTAGAGGTGGCAGCCTCTATCAACAACTACATGCCAGTACATGTTGTCAACCTCGTCGAGGAG
GGCCTTGCAAAGCTAGGCAAGAGGATTAGGGGGGCCCGGGTAGCAGTTCTAGGCCTTGCCTACAAGCCCGATGTCAACGA
CGCCAGGGAATCCCCTTCAATAAGAATCATCGAAGAGCTAGTGGAGCGGGGCGCAGTTGTTAGGGTTCACGACCCCCTGG
TAGAGAGTGTTAAGACAGTCTACGGAGTCTTCCAGAGCACTGTGGACCCCCTCGAGCTAGTAGGCTGGGCCGATGCTGTT
GTAATAGCTGTCAACCATACAGCCTACCACAGCAAGGAGCTCGTGACAAAGCTGCTCAACCCAGGTAAGGAGCTAGTAGT
GGTTGACACGAGGAACCTTCTGAGCGGCGCTAGGCCGGGGCCGGGTGTGGTACTGCTAAGGCTTGGAGCCCCCCACAGCT
AG

Upstream 100 bases:

>100_bases
GCTGGCTACGCGGGTCCATGGGCTTCCCGGTGGCTGGCCGCTCCCCAGATAAACCTCGCCTAGGCCTCGTAGCCTTGAGG
CTGGGGGCCGGTAGGGGGTC

Downstream 100 bases:

>100_bases
GCCTAGCAGCCTCAGCAGCATCATCCTAGCCCAGGAGTTAGTGCTAGCACAAGCCCCGTATAGGCCGGCGAGAAGCACAG
ACAAGGCCAGGGAATAGGGA

Product: polysaccharide biosynthesis protein

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MQPGSWLDELREKLRENRVVVGVVGLGYVGLPLSLLYASKGFRVIGYDIDQGRVEAIRRGRSYINYIPDHEVARVVKEGL
LEAYTSPEPLHEADFILVTVPTPLRCNGLPDLSYVRSAARSVGRVLRRGQVVVLESSTYPGTTREVLAPILEEESGLRVV
RDFGVAYSPERINPGDTRYKLEDIPKVVGGITPEITDIVAEFYSKAFKYVVKVKDCKTAEATKMLENMFRYINIALVNEL
AVAFEKMGINVWEVIEAAKTKPFGFMAFYPGPGVGGHCIPVDPFYLRYSAWRHGAVLRLVEVAASINNYMPVHVVNLVEE
GLAKLGKRIRGARVAVLGLAYKPDVNDARESPSIRIIEELVERGAVVRVHDPLVESVKTVYGVFQSTVDPLELVGWADAV
VIAVNHTAYHSKELVTKLLNPGKELVVVDTRNLLSGARPGPGVVLLRLGAPHS

Sequences:

>Translated_453_residues
MQPGSWLDELREKLRENRVVVGVVGLGYVGLPLSLLYASKGFRVIGYDIDQGRVEAIRRGRSYINYIPDHEVARVVKEGL
LEAYTSPEPLHEADFILVTVPTPLRCNGLPDLSYVRSAARSVGRVLRRGQVVVLESSTYPGTTREVLAPILEEESGLRVV
RDFGVAYSPERINPGDTRYKLEDIPKVVGGITPEITDIVAEFYSKAFKYVVKVKDCKTAEATKMLENMFRYINIALVNEL
AVAFEKMGINVWEVIEAAKTKPFGFMAFYPGPGVGGHCIPVDPFYLRYSAWRHGAVLRLVEVAASINNYMPVHVVNLVEE
GLAKLGKRIRGARVAVLGLAYKPDVNDARESPSIRIIEELVERGAVVRVHDPLVESVKTVYGVFQSTVDPLELVGWADAV
VIAVNHTAYHSKELVTKLLNPGKELVVVDTRNLLSGARPGPGVVLLRLGAPHS
>Mature_453_residues
MQPGSWLDELREKLRENRVVVGVVGLGYVGLPLSLLYASKGFRVIGYDIDQGRVEAIRRGRSYINYIPDHEVARVVKEGL
LEAYTSPEPLHEADFILVTVPTPLRCNGLPDLSYVRSAARSVGRVLRRGQVVVLESSTYPGTTREVLAPILEEESGLRVV
RDFGVAYSPERINPGDTRYKLEDIPKVVGGITPEITDIVAEFYSKAFKYVVKVKDCKTAEATKMLENMFRYINIALVNEL
AVAFEKMGINVWEVIEAAKTKPFGFMAFYPGPGVGGHCIPVDPFYLRYSAWRHGAVLRLVEVAASINNYMPVHVVNLVEE
GLAKLGKRIRGARVAVLGLAYKPDVNDARESPSIRIIEELVERGAVVRVHDPLVESVKTVYGVFQSTVDPLELVGWADAV
VIAVNHTAYHSKELVTKLLNPGKELVVVDTRNLLSGARPGPGVVLLRLGAPHS

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=451, Percent_Identity=24.8337028824834, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI296040438, Length=352, Percent_Identity=25, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI296040443, Length=239, Percent_Identity=25.9414225941423, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI48994968, Length=406, Percent_Identity=36.6995073891626, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1788340, Length=269, Percent_Identity=26.0223048327138, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17560350, Length=375, Percent_Identity=27.2, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17136908, Length=382, Percent_Identity=28.0104712041885, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 49990; Mature: 49990

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPGSWLDELREKLRENRVVVGVVGLGYVGLPLSLLYASKGFRVIGYDIDQGRVEAIRRG
CCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHC
RSYINYIPDHEVARVVKEGLLEAYTSPEPLHEADFILVTVPTPLRCNGLPDLSYVRSAAR
CHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
SVGRVLRRGQVVVLESSTYPGTTREVLAPILEEESGLRVVRDFGVAYSPERINPGDTRYK
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHCCCEECCCCCCCCCCCEE
LEDIPKVVGGITPEITDIVAEFYSKAFKYVVKVKDCKTAEATKMLENMFRYINIALVNEL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVAFEKMGINVWEVIEAAKTKPFGFMAFYPGPGVGGHCIPVDPFYLRYSAWRHGAVLRLV
HHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHCCHHHHHHH
EVAASINNYMPVHVVNLVEEGLAKLGKRIRGARVAVLGLAYKPDVNDARESPSIRIIEEL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHCCCCHHHHHHH
VERGAVVRVHDPLVESVKTVYGVFQSTVDPLELVGWADAVVIAVNHTAYHSKELVTKLLN
HHCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCHHHHHHHHHHHHC
PGKELVVVDTRNLLSGARPGPGVVLLRLGAPHS
CCCEEEEEECHHHHCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MQPGSWLDELREKLRENRVVVGVVGLGYVGLPLSLLYASKGFRVIGYDIDQGRVEAIRRG
CCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHC
RSYINYIPDHEVARVVKEGLLEAYTSPEPLHEADFILVTVPTPLRCNGLPDLSYVRSAAR
CHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
SVGRVLRRGQVVVLESSTYPGTTREVLAPILEEESGLRVVRDFGVAYSPERINPGDTRYK
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHCCCEECCCCCCCCCCCEE
LEDIPKVVGGITPEITDIVAEFYSKAFKYVVKVKDCKTAEATKMLENMFRYINIALVNEL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVAFEKMGINVWEVIEAAKTKPFGFMAFYPGPGVGGHCIPVDPFYLRYSAWRHGAVLRLV
HHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHCCHHHHHHH
EVAASINNYMPVHVVNLVEEGLAKLGKRIRGARVAVLGLAYKPDVNDARESPSIRIIEEL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHCCCCHHHHHHH
VERGAVVRVHDPLVESVKTVYGVFQSTVDPLELVGWADAVVIAVNHTAYHSKELVTKLLN
HHCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCHHHHHHHHHHHHC
PGKELVVVDTRNLLSGARPGPGVVLLRLGAPHS
CCCEEEEEECHHHHCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]