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The map label for this gene is hydA [H]

Identifier: 124028221

GI number: 124028221

Start: 1362519

End: 1364039

Strand: Reverse

Name: hydA [H]

Synonym: Hbut_1371

Alternate gene names: 124028221

Gene position: 1364039-1362519 (Counterclockwise)

Preceding gene: 124028225

Following gene: 124028220

Centisome position: 81.82

GC content: 54.44

Gene sequence:

>1521_bases
GTGCTAGAACTAAGGCTGTCTAGGAGACAGTTTCTAAAGCTTGCTGGTACCACAGCGCTACTAGCATCTCTTGACTGGCG
TAAGCTAATACGCAGAGCCTTTGCAGAACTGGAGACGGGAGGTATTAGCCTTGTATGGTTTGAAGCCCAGGACTGCGCTG
GTGATACAACGTCCTTGATACAGGCTACCGAGCCAGACATTGTTGAGGTTCTAGCTGGTCTTACAAATCTAACACCACCG
GGTGCTGTTGTACTACCATTCCATGAGACTGTTATGCCCGAATGGGGCTTCGGCTCTATAGCGCATCTTCTGTTAGCCCT
GGGTGGCGTATACGATCCATACGTGCTCATTCTAGAAGGGGCCATGCCACCGGACGAGGATCTAACCGGCATTAAGGGCA
CAAACATGCTGTGCTACATTGGTGACTACGAGACTGTTATGGAGTCGCTTGAGAAGCTCCGCAGGGTAGTTGAGGGCAAG
ATGAAGCCCAACACACTGCTCCCAGTGGTTGACGCTGTCAAGGCCATATCGCCGGAGAGAGCCAAGATTATCGCTGAGAG
GCTCGTGGAGAAGATTGACGAGGTTGAGAAGGCGACAGAAGAGTGCATGAAGGGGCTTAGTGGCGAGGATGTCGTCACTT
GTGCAAGGTGGTTTGCCTGCCTTATGAAGCGCGCCATAGGAGTTGTTAGCTTTGGCAACTGTGCAAGCTACGGTGGCGTT
GTCGGCAACACCGTAGCTTTCAGCTGGGCTCGCAGCGACCTGCTAGAGGATTACGAGTCAACAACAGGCTACAAACTGTA
TAGGGAATGGGCCAGGTATGGATGGAGCTTTAGTCCAACCGGCGGTGTAGGCTTCTTCCCCGACCCCGTTAGGGGCTACA
AGGGCTTCGCAAAGATACTAGCTGAACAAGCGCCAGAGCCATTTAGAAGCTTTGCGAAGCCCTACGTGGACTTTATCGAT
GGCAAGTGTGTACCCGACAAGCCAAGCCCCGATGCAAGCTGCAAGCCCGCCGTAGCTGTACCAGGATGCCCCTCCAACGG
CAATGCGCAACTGAAGACCCTGACAAACCTCATACTATGGGTTAAGGGAGAGCTACCTCTACCAGAGCTTGACCAGTACT
GGAGGCCAAAGATCATCTTTGGAGGCTCTGTACACGAGCAATGCCCGAGAAATGAAAAGTACCGTGCAGGTATACTACGC
AGCGAGCCAGGCGAGCCTAGCGGCGCATGCCTCTTCAGCGTCGGCTGCAAAGGCCCCATATCAAATTGTCCATACAACAA
GATTGGCTGGGTTAATGGTGTCGGCGGTCCAACTAGGACCGGCGGTGTCTGCATAGGCTGCACGATGCCAGGCTTCATGG
ACGCCTATGAGCCGTACTACAAGCCGTTACCAGCCCCCGTGAAGCCTGAAGCCTCCGCTGCAACCGAGCTCGCAGCCGGC
GCGGCTGTTGTTGGTGTACTCGCAGCTTATGGTATCCGTAAGCTCGCGGGAGGTGAGAAGGAGAAGGAGAAGGGCGCGTG
A

Upstream 100 bases:

>100_bases
ATAGTGTTATACTAATTGAGTGTTTACAGCGTTACCTTTATTTACTCTACACAAACTTAAAATAACTACTTGATCATGCT
GAGACGGGGTGTGCTCAAGA

Downstream 100 bases:

>100_bases
AGGGGGTGGGCTCCGTGGCGTTGGGCGACATTATCTACACGTTCGCTGTGTATAAGTTCTTCTGGATTGCCATAGCTGTA
TTTGCCTTTGGTGTAGCCTA

Product: NiFe hydrogenase

Products: NA

Alternate protein names: NiFe hydrogenlyase small chain [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLELRLSRRQFLKLAGTTALLASLDWRKLIRRAFAELETGGISLVWFEAQDCAGDTTSLIQATEPDIVEVLAGLTNLTPP
GAVVLPFHETVMPEWGFGSIAHLLLALGGVYDPYVLILEGAMPPDEDLTGIKGTNMLCYIGDYETVMESLEKLRRVVEGK
MKPNTLLPVVDAVKAISPERAKIIAERLVEKIDEVEKATEECMKGLSGEDVVTCARWFACLMKRAIGVVSFGNCASYGGV
VGNTVAFSWARSDLLEDYESTTGYKLYREWARYGWSFSPTGGVGFFPDPVRGYKGFAKILAEQAPEPFRSFAKPYVDFID
GKCVPDKPSPDASCKPAVAVPGCPSNGNAQLKTLTNLILWVKGELPLPELDQYWRPKIIFGGSVHEQCPRNEKYRAGILR
SEPGEPSGACLFSVGCKGPISNCPYNKIGWVNGVGGPTRTGGVCIGCTMPGFMDAYEPYYKPLPAPVKPEASAATELAAG
AAVVGVLAAYGIRKLAGGEKEKEKGA

Sequences:

>Translated_506_residues
MLELRLSRRQFLKLAGTTALLASLDWRKLIRRAFAELETGGISLVWFEAQDCAGDTTSLIQATEPDIVEVLAGLTNLTPP
GAVVLPFHETVMPEWGFGSIAHLLLALGGVYDPYVLILEGAMPPDEDLTGIKGTNMLCYIGDYETVMESLEKLRRVVEGK
MKPNTLLPVVDAVKAISPERAKIIAERLVEKIDEVEKATEECMKGLSGEDVVTCARWFACLMKRAIGVVSFGNCASYGGV
VGNTVAFSWARSDLLEDYESTTGYKLYREWARYGWSFSPTGGVGFFPDPVRGYKGFAKILAEQAPEPFRSFAKPYVDFID
GKCVPDKPSPDASCKPAVAVPGCPSNGNAQLKTLTNLILWVKGELPLPELDQYWRPKIIFGGSVHEQCPRNEKYRAGILR
SEPGEPSGACLFSVGCKGPISNCPYNKIGWVNGVGGPTRTGGVCIGCTMPGFMDAYEPYYKPLPAPVKPEASAATELAAG
AAVVGVLAAYGIRKLAGGEKEKEKGA
>Mature_506_residues
MLELRLSRRQFLKLAGTTALLASLDWRKLIRRAFAELETGGISLVWFEAQDCAGDTTSLIQATEPDIVEVLAGLTNLTPP
GAVVLPFHETVMPEWGFGSIAHLLLALGGVYDPYVLILEGAMPPDEDLTGIKGTNMLCYIGDYETVMESLEKLRRVVEGK
MKPNTLLPVVDAVKAISPERAKIIAERLVEKIDEVEKATEECMKGLSGEDVVTCARWFACLMKRAIGVVSFGNCASYGGV
VGNTVAFSWARSDLLEDYESTTGYKLYREWARYGWSFSPTGGVGFFPDPVRGYKGFAKILAEQAPEPFRSFAKPYVDFID
GKCVPDKPSPDASCKPAVAVPGCPSNGNAQLKTLTNLILWVKGELPLPELDQYWRPKIIFGGSVHEQCPRNEKYRAGILR
SEPGEPSGACLFSVGCKGPISNCPYNKIGWVNGVGGPTRTGGVCIGCTMPGFMDAYEPYYKPLPAPVKPEASAATELAAG
AAVVGVLAAYGIRKLAGGEKEKEKGA

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=150, Percent_Identity=29.3333333333333, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789371, Length=123, Percent_Identity=34.9593495934959, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 54695; Mature: 54695

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELRLSRRQFLKLAGTTALLASLDWRKLIRRAFAELETGGISLVWFEAQDCAGDTTSLI
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
QATEPDIVEVLAGLTNLTPPGAVVLPFHETVMPEWGFGSIAHLLLALGGVYDPYVLILEG
HCCCCHHHHHHHHHHCCCCCCEEEECCHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
AMPPDEDLTGIKGTNMLCYIGDYETVMESLEKLRRVVEGKMKPNTLLPVVDAVKAISPER
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHH
AKIIAERLVEKIDEVEKATEECMKGLSGEDVVTCARWFACLMKRAIGVVSFGNCASYGGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEECCCHHHCCCC
VGNTVAFSWARSDLLEDYESTTGYKLYREWARYGWSFSPTGGVGFFPDPVRGYKGFAKIL
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AEQAPEPFRSFAKPYVDFIDGKCVPDKPSPDASCKPAVAVPGCPSNGNAQLKTLTNLILW
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHEE
VKGELPLPELDQYWRPKIIFGGSVHEQCPRNEKYRAGILRSEPGEPSGACLFSVGCKGPI
EECCCCCCCHHHHCCCEEEECCCHHHHCCCCCCHHCCCCCCCCCCCCCCEEEEECCCCCC
SNCPYNKIGWVNGVGGPTRTGGVCIGCTMPGFMDAYEPYYKPLPAPVKPEASAATELAAG
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AAVVGVLAAYGIRKLAGGEKEKEKGA
HHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MLELRLSRRQFLKLAGTTALLASLDWRKLIRRAFAELETGGISLVWFEAQDCAGDTTSLI
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
QATEPDIVEVLAGLTNLTPPGAVVLPFHETVMPEWGFGSIAHLLLALGGVYDPYVLILEG
HCCCCHHHHHHHHHHCCCCCCEEEECCHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
AMPPDEDLTGIKGTNMLCYIGDYETVMESLEKLRRVVEGKMKPNTLLPVVDAVKAISPER
CCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHH
AKIIAERLVEKIDEVEKATEECMKGLSGEDVVTCARWFACLMKRAIGVVSFGNCASYGGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCEEECCCHHHCCCC
VGNTVAFSWARSDLLEDYESTTGYKLYREWARYGWSFSPTGGVGFFPDPVRGYKGFAKIL
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AEQAPEPFRSFAKPYVDFIDGKCVPDKPSPDASCKPAVAVPGCPSNGNAQLKTLTNLILW
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHEE
VKGELPLPELDQYWRPKIIFGGSVHEQCPRNEKYRAGILRSEPGEPSGACLFSVGCKGPI
EECCCCCCCHHHHCCCEEEECCCHHHHCCCCCCHHCCCCCCCCCCCCCCEEEEECCCCCC
SNCPYNKIGWVNGVGGPTRTGGVCIGCTMPGFMDAYEPYYKPLPAPVKPEASAATELAAG
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AAVVGVLAAYGIRKLAGGEKEKEKGA
HHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3322743; 2651421; 3322275; 3134950; 7854413 [H]