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The map label for this gene is pcm [C]

Identifier: 124028204

GI number: 124028204

Start: 1343733

End: 1344536

Strand: Reverse

Name: pcm [C]

Synonym: Hbut_1352

Alternate gene names: 124028204

Gene position: 1344536-1343733 (Counterclockwise)

Preceding gene: 124028205

Following gene: 124028203

Centisome position: 80.65

GC content: 63.81

Gene sequence:

>804_bases
TTGAGTGGCGAGTGCTGCCGCGACATAGTCTGTGAGGGCTGCCCCGTCCTCCTTGTAATCGATGAGAGGAGGCGCTTCAT
CTTCCGTGTCCGGCGCGGCGCTAGGCTCGGCAGCGACCGTGGAGTACTACGCCACGACGACGTGATAGGCCTCCCCTACG
GGTCGAGGGTGAGGCTCTCCACCGGCGTCGAGGCCGTGGTTCTCCAGCCACGCCTTGTCGACTATATGGAGCGGGGGCTT
GAGAGGCGGAGCCAGGTCATATACCCGAAGGATCACGGCCTCATAGTGATGCTGCTGGATCTCAGGCCCGGCATGAGGGT
CCTGGAGGTCGGCGTGGGGAGCGGCTATACCACGGCTGTCCTGGCATCGATAGTGGGGCCCGAGGGCCACGTCTACAGCT
ACGAGATAAGAGGGGACATGGCTGAGACGGCTCGCCGCAACCTAGAGAGGCTGGGCCTGCTCGACCGCGTAACGATACGG
GTCCGCGACGCGAGGCAGGGGATAGACGAGAGAGACCTGGACGCCGCTGTCGTGGACATGCCCGACCCCTGGAGCATCCT
GGAGCACCTACACAAAGCGCTGCGCCCAGGCGCTGGAGCAGTCTTCTTCATGCCTGCAGCGAACCAGGTGGCGAGACTTC
TACAGGCGCTGGAGCTGAGCGGGGCCTGGACCGAGACCAGGGTCTACGAGGTACTGTTGCGCGAGTACGAGGCCAGGAGC
GACGCGCTACGCCCCAGGACGACGATGATAGCCCACACGGGCTACATACTATACACGCGGAAGCTCCAAGCGCAGCAAAC
CTAA

Upstream 100 bases:

>100_bases
GGGCGTAGTGTACCGCTGCCCGAAGTGCGGCTTCGAGGGTCCATGAGCGTAGCCGTCTAGCCCTATTCTCGCCTCCTCGC
CAGTGTAGGGAGTGCGTGTT

Downstream 100 bases:

>100_bases
AACCACACCCACACATATGATATGTAGACTACATGGCTGCGGGGGTGCCCGAGCCAGGTCGAAGGGGGCGGGCTCAAGAC
CCGCTGGCGTAGGCCGGCGT

Product: tRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSGECCRDIVCEGCPVLLVIDERRRFIFRVRRGARLGSDRGVLRHDDVIGLPYGSRVRLSTGVEAVVLQPRLVDYMERGL
ERRSQVIYPKDHGLIVMLLDLRPGMRVLEVGVGSGYTTAVLASIVGPEGHVYSYEIRGDMAETARRNLERLGLLDRVTIR
VRDARQGIDERDLDAAVVDMPDPWSILEHLHKALRPGAGAVFFMPAANQVARLLQALELSGAWTETRVYEVLLREYEARS
DALRPRTTMIAHTGYILYTRKLQAQQT

Sequences:

>Translated_267_residues
MSGECCRDIVCEGCPVLLVIDERRRFIFRVRRGARLGSDRGVLRHDDVIGLPYGSRVRLSTGVEAVVLQPRLVDYMERGL
ERRSQVIYPKDHGLIVMLLDLRPGMRVLEVGVGSGYTTAVLASIVGPEGHVYSYEIRGDMAETARRNLERLGLLDRVTIR
VRDARQGIDERDLDAAVVDMPDPWSILEHLHKALRPGAGAVFFMPAANQVARLLQALELSGAWTETRVYEVLLREYEARS
DALRPRTTMIAHTGYILYTRKLQAQQT
>Mature_266_residues
SGECCRDIVCEGCPVLLVIDERRRFIFRVRRGARLGSDRGVLRHDDVIGLPYGSRVRLSTGVEAVVLQPRLVDYMERGLE
RRSQVIYPKDHGLIVMLLDLRPGMRVLEVGVGSGYTTAVLASIVGPEGHVYSYEIRGDMAETARRNLERLGLLDRVTIRV
RDARQGIDERDLDAAVVDMPDPWSILEHLHKALRPGAGAVFFMPAANQVARLLQALELSGAWTETRVYEVLLREYEARSD
ALRPRTTMIAHTGYILYTRKLQAQQT

Specific function: Catalyzes The Methyl Esterification Of L-Isoaspartyl Residues In Peptides And Proteins That Result From Spontaneous Decomposition Of Normal L-Aspartyl And L-Asparaginyl Residues. It Plays A Role In The Repair And/Or Degradation Of Damaged Proteins. This

COG id: COG2519

COG function: function code J; tRNA(1-methyladenosine) methyltransferase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TRM61 family [H]

Homologues:

Organism=Homo sapiens, GI123173772, Length=249, Percent_Identity=34.5381526104418, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI222831587, Length=230, Percent_Identity=26.0869565217391, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509459, Length=218, Percent_Identity=30.7339449541284, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6322336, Length=274, Percent_Identity=31.021897810219, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24650257, Length=211, Percent_Identity=33.175355450237, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014816 [H]

Pfam domain/function: PF08704 GCD14 [H]

EC number: =2.1.1.36 [H]

Molecular weight: Translated: 30094; Mature: 29963

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGECCRDIVCEGCPVLLVIDERRRFIFRVRRGARLGSDRGVLRHDDVIGLPYGSRVRLS
CCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEECCCEEECCCCCEEEEC
TGVEAVVLQPRLVDYMERGLERRSQVIYPKDHGLIVMLLDLRPGMRVLEVGVGSGYTTAV
CCCEEEEECHHHHHHHHHHHHHHCCEECCCCCCEEEEEEECCCCCEEEEEECCCCHHHHH
LASIVGPEGHVYSYEIRGDMAETARRNLERLGLLDRVTIRVRDARQGIDERDLDAAVVDM
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCHHHEEEEEEHHHCCCCCCCCCEEEECC
PDPWSILEHLHKALRPGAGAVFFMPAANQVARLLQALELSGAWTETRVYEVLLREYEARS
CCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DALRPRTTMIAHTGYILYTRKLQAQQT
CCCCCCCEEEEECCEEEEEEEECCCCC
>Mature Secondary Structure 
SGECCRDIVCEGCPVLLVIDERRRFIFRVRRGARLGSDRGVLRHDDVIGLPYGSRVRLS
CCHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEECCCEEECCCCCEEEEC
TGVEAVVLQPRLVDYMERGLERRSQVIYPKDHGLIVMLLDLRPGMRVLEVGVGSGYTTAV
CCCEEEEECHHHHHHHHHHHHHHCCEECCCCCCEEEEEEECCCCCEEEEEECCCCHHHHH
LASIVGPEGHVYSYEIRGDMAETARRNLERLGLLDRVTIRVRDARQGIDERDLDAAVVDM
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCHHHEEEEEEHHHCCCCCCCCCEEEECC
PDPWSILEHLHKALRPGAGAVFFMPAANQVARLLQALELSGAWTETRVYEVLLREYEARS
CCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DALRPRTTMIAHTGYILYTRKLQAQQT
CCCCCCCEEEEECCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]